• 제목/요약/키워드: latex agglutination test

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도축 타조에서 닭 및 돼지 질병에 대한 혈청학적 조사 (Serological survey of diseases to poultry and swine in slaughtered ostriches)

  • 김순태;박인화;김영환;조광현;오규실;손재권;정종식
    • 한국동물위생학회지
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    • 제27권3호
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    • pp.281-288
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    • 2004
  • As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.

Seroepidemiological study of Toxoplasma gondii infection in the rural area Okcheon-gun, Korea

  • Lee, Young-Ha;Hoh, Hyung-Jun;Hwang, Ok-Sun;Lee, Sang-Keol;Shin, Dae-Whan
    • Parasites, Hosts and Diseases
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    • 제38권4호
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    • pp.251-256
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    • 2000
  • There have been some reports about the prevalence of anti-Toxoplasma gondii antibody among Koreans, and most of all data were taken from patients visiting hospitals. However, the epidemiological data of the community-based study in Korea are rare. This study was performed to evaluate the seroprevalence of toxoplasmosis among the inhabitants of the rural area Okcheon-gun, Korea. A total of 1,109 serum samples (499 males, 610 females) were examined for the IgG antibodies by ELISA. To set up the cut-off point for ELISA, we used a commercial latex agglutination (LA) kit. The sensitivity and specificity of ELISA against LA test were 89.5%, and 98.6% respectively. Among 1,109 sera, 6.9% showed seropositivity by ELISA. The positive rates of males and females were 6.0% and 7.2%, respectively. However, there were no significant differences between sexes. Comparing the age groups, the highest seropositive rate showed in the seventies or higher, and their rates had a tendency to increase with age (0.05 < p < 0.3). These results revealed that the seroprevalence of toxoplasmosis in rural inhabitants is similar to previous reports in Korea; however we need further investigation to clarify the prevalence of toxoplasmosis in the general population.

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서울지역 길고양이의 Toxoplasma 감염증 실태조사 (Investigation of stray cats Toxoplasmosis in Seoul area)

  • 김능희;채희선;한혜진;손홍락;김창기;김선홍;이정학;김철훈
    • 한국동물위생학회지
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    • 제32권3호
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    • pp.275-279
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    • 2009
  • Toxoplasma gondii is one of the most common protozoa parasites of human and other warm-blooded animals. Cats and wild felidae play crucial roles in the epidemiology of toxoplasmosis. This study was performed to survey the prevalence of T. gondii infection among stray cats in the Seoul. A total of 422 stray cat blood samples were collected from Seoul area. Positive sera for T. gondii were identified in 56 samples (13.3%) exclusively via the latex agglutination test and the detection no antigen particles among seropositive samples by PCR. The overall infection rate of male stray cats (14.4%) presented as higher than that of female cats (10.7%). This study suggested that T. gondii is widespread in stray cats of Seoul area. It is needed to control urban stray cat population and to reduced the risk of zoonotic transmission of toxolasmosis to other animals and humans.

Toxoplasma gondii antibody titers in sera of children admitted to the Seoul National University Children's Hospital

  • Kook, Jina;Lee, Hong-Jin;Kim, Beyong-Il;Yun, Chong-Ku;Guk, Sang-Mee;Seo, Min;Park, Yun-Kyu;Hong, Sung-Tae;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • 제37권1호
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    • pp.27-32
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    • 1999
  • A total of 542 children under 10 years of age, admitted to the Seoul National University Children's Hospital, was examined for antibody titers of Toxoplasma gondii using indirect latex agglutination (ILA) test. Among them, 7.7% showed positive titers higher than 1:32, without significant difference between males (7.3%) and females (8.5%). The seropositive rate increased with age although the statistical significance was negligible (0.05

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동물원 동물에서의 Latex응집반응에 의한 Toxoplasmu항체가 (Toxoplasma Antibodies by Indirect Latex Agglutination Tests in Zoo Animals)

  • 최원영;유재을
    • Parasites, Hosts and Diseases
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    • 제25권1호
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    • pp.13-23
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    • 1987
  • 서울대공원에서 사육중인 각종동물을 대상으로 Toxoplasma의 보유숙주로서의 가능성을 간접 Latex 응집반응을 이용하여 항체가를 검토하였다. 포유류, 조류 및 파충류중 가능한 범위내에서 PKU여과지에 혈액을 흡착시킨 후 건조시켜서 조사하였다. 131개체의 포유류에서 20개체 (15.3%), 조류에서는 75개체에서 2개체 (2.7%)가 Toxoplasma 양성 항체가를 가진 것으로 나타났으며, 종별로는 68종의 포유류에서 15종(22. %), 36종의 조류에서 2종(5.6%)이었으며, 조사한 10개체 (7종)의 파충류는 모두 음성으로 나타났다. 포유류에서는 캉가루목의 6개체에서 2개체 (3종중 1종), 원숭이 목의 15개체중 1개체 (11종증 1종), 박쥐목의 1개체 (1종), 개목의 13개체중 6개체 (10종중 5종) 말목의 12개체중 1개체 (3종중 1종), 소목의 80개체 중 9개체 (38종중 6종)가 양성으로 나타났으며, 쥐목과 고래 목에서는 음성이었다. 조류에서는 닭목에서 1개체, 앵무목에서 1개체만이 양성이었다. 항체가별로는 포유류에서 1 : 64가 9개체, 1 : 32가 6개체, 1 : 128이 3개체, 1 256이 2개체로 나타났으며, 조류에서의 2 양성 개체는 모두 1 : 64의 항체가를 보였다.

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The Occurrence and Molecular Characterization of Feline Cryptococcosis in Korea

  • Kim, Yoon Hee;Song, Ae-ra;Hwang, Soo Myung;Hong, Yeon-Jung;Choi, Ul Soo
    • 한국임상수의학회지
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    • 제35권4호
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    • pp.170-173
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    • 2018
  • A spayed female domestic short-hair cat of unknown age was admitted with a large proliferative mass in the face. Cytology and biopsy results suggested infection with Cryptococcus spp. A latex cryptococcal antigen agglutination test and an ALPHA cryptococcal antigen enzyme immunoassay yielded positive results. Results of canavanine-glycine-bromothymol blue agar test, serotyping and molecular typing by URA5 - RFLP and MLST analysis identified the isolates as C. neoformans var. grubii VNI/ST31. Two other cats were also diagnosed with the same methodology showing Crytococcosis with VNI/ST31. Cats presenting with facial or respiratory signs should be assessed for cryptococcosis in Korea.

포유자돈 소장에서 분리된 대장균의 섬모항원과 장내독소 분포양상 (Prevalence of pili and enterotoxins of Escherichia coli associated with diarrhea in preweaning piglets)

  • 함희진;천두성;채찬희
    • 대한수의학회지
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    • 제37권4호
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    • pp.779-784
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    • 1997
  • A comprehensive study of 132 Escherichia coli isolates from 150 piglets with colibacillosis included detection of heat-labile enterotoxin, heat-stable enterotoxin, and identification of K88 (F4), K99 (F5), 987P (F6), and F41. Four pili were examined by haemagglutination and slide agglutination test. Heat-labile(LT) and heat-stable(ST) enterotoxin was determined by reverse passive latex agglutination and precipitation test, respectively. Among 132 E coli isolates, 26 had K88 (19.7%), 16 had K99 (12.1%), 3 had 987P (2.3%), and 2 had F41 (1.5%). Three had K88 and K99 (2.3%), 3 had K88 and 987P (2.3%), 2 had K99 and 987P (1.5%), 5 had K99 and F41 (3.8%), and 8 E coli strains had K88, K99 and F41 (6.1%) simultaneously. Among 132 E coli isolates, 5 produced LT only (3.8%), 55 produced heat-stable toxin ST only (41.7%), and 4 produced both LT and ST (3.0%). Three major pathotypes accounted for 27.9% of E coli isolates: $K99^+$ (8.3%), $K88^+ST^+$ (9%) and $K88^+$ (10.6%). Results of this study indicated that piliated enterotoxin-producing E coli was prevalent and was associated with diarrhea in preweaning piglets.

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도축돈에서의 Haemophilus Pleuropneumoniae 감염에 관한 연구 (Studios on Infection of Haemophilus Pleuropneumoniae in Slaughtered Pigs)

  • 이학철;유병삼
    • 한국임상수의학회지
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    • 제3권1호
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    • pp.243-253
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    • 1986
  • In recent years much attention has been paid to swine respiratory infection caused by Haemophilus(H.) Pleuropneumoniae with rapid expansion of pork industry in Korea. The organism may cause an acute respiratory infection with high morbidity and mortality, or it may also cause chronic persistent infection, resulting in loss of body weight. The present study was performed to observe the etiologic situation of H. Pleuropneumoniae infection by evaluating bacteriological, serological examinations with the lungs and sera collected from slaughtered pigs in Daegu city, respectively. In addition, antibiotic susceptibility test was carried out against the isolated strains of H. Pleuropneumoniae. The results obtained are summarized as follows : 1. Only 10 strains of H. Pleuropneumoniae were isolated from the 120 cases of swine lungs examined, and biological and biochemical characteristics of the isolates were identical with that of standard strains(serovar 1 to 6). 2. Out of 336 swine sera examined, 108(32.1%) were shown to be positive against H. Pleuropneumoniae by latex agglutination test. 3. Antibiotic susceptibility test revealed that 10 isolates of H. Pleuropneumoniae were sensitive to 16 kinds of antibiotic such as ampicillin, benzylpenicillin, piperacillin, cephalothin, cefazolin, cefamandol, cefoperagon, lactomoxycefa, tetracycline, minocycline, chloramphenicol, gentamicin, kanamycin, amikacin, colistin and nalidixic acid, whereas cloxacillin, erythromycin, kitasamycin, lincomycin, oleand-amycin, and bacitracin were resistent.

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