• Title/Summary/Keyword: lateral flow

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Influence of Intracerebroventricular Nalorphine on the Renal Function of the Rabbit (측뇌실내(側腦室內) Nalprphine의 가토신장기능(家兎腎臟機能)에 미치는 영향(影響))

  • Kook, Young-Johng;Choi, Bong-Kyu;Kim, Heung-Kyu
    • The Korean Journal of Pharmacology
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    • v.16 no.2 s.27
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    • pp.1-7
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    • 1980
  • As it has been reported that morphine induce antidiuresis, and antinatriuresis along with decrease in renal hemodynamics when given intracerebroventricularly[ivt], the renal action of nalorphine, a partial antagonist of morphine action, and its influence upon the morphine action were investigated in this study. $10{\mu}g/kg$ of nalorphine given into the lateral ventricle of the rabbit brain tended to decrease renal plasma flow and glomerular filtration rate and increase the reabsorption of free water in the tubules. $100{\mu}g/kg$ ivt significantly decreased urine flow rate and increased free water reabsorption, and tended to increase electrolyte excretion in spite of decrease in renal plasma flow and glomerular filtration, suggesting that ADH also involved in the antidiuresis. Morphine hydrochloride, $10{\mu}g/kg$, ivt, produced marked decrement in renal hemodynamics along with decreased excretions of sodium, potassium and water, and these morphine actions were alleviated by nalorphine given 20 min later. The natriuretic action of ivt nalorphine manifested itself uninfluenced by the morphine. These observations indicate that nalorphine ivt produces renal actions similar to those of morphine, though less potent, and that it can antagonize the latter action. It is suggested that morphine influences renal hemodynamics through nerve by stimulating the 'morphine receptor' in the brain, whereas nalorphine liberates ADH by the agonistic action on the 'nalorphine receptor'.

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Oscillatory Josephson-Vortex Resistance in Stacks of $Bi_{2}Sr_{2}CaCu_{2}O_{8+x}$ Intrinsic Josephson Junctions

  • Choi Jae-Hyun;Bae Myung-Ho;Lee Hu-Jong;Kim Sang-Jae
    • Progress in Superconductivity
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    • v.7 no.1
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    • pp.17-21
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    • 2005
  • We report the oscillation of the Josephson vortex-flow resistance in the rectangular stacks of $Bi_{2}Sr_{2}CaCu_{2}O_{8+x}$(Bi-2212) intrinsic Josephson junctions (IJJs). Apiece of Bi-2212 single crystal containing a few tens of IJJs was sandwiched between two gold electrodes and fabricated into a rectangular shape with the typical lateral size of about $1.5{\times}10\;{\mu}m^2$, using e-beam lithography and focused ion-beam etching techniques. In a tesla-range magnetic field applied in parallel with the junction planes, the oscillation of the Josephson vortex flow resistance was observed at temperatures near 60 K. The oscillation results from the interplay between the triangular Josephson vortex lattice and the potential barrier at the boundary of a single crystal. The oscillatory magnetoresistance for different bias currents, external magnetic fields, and the tilt-angles provides useful information on the dynamics of the coupled Josephson-vortex lattice system.

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Transcervical Embryo Recovery in Korean Black Goats: A Preliminary Experiment

  • Lee, Doo-Soo;Kim, Dong-Woo;Shin, Sang Tae
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.171-174
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    • 2015
  • Four pluriparous Korean black goat does were superovulated with FSH and mated with fertile bucks. Anesthetized animals were placed in lateral recumbency, then size 8 Foley catheter was inserted into the uterus through the cervix under the vaginal speculum and the balloon was inflated to fix the catheter in the uterine body. The opposite end of the catheter was connected to a 3-way and a flushing medium was infused into the uterus. Modified Dubecco's PBS with 1% FBS was used as the flushing medium. Four goats were allocated in two groups depending on the type of medium infusion into uterus. Injection group; the flushing medium was injected into uterus and the infused medium was collected by to-and-fro method using a syringe. Gravity-flow group; the flushing medium was allowed to enter the uterus by gravity flow by lifting the medium bottle and drained out of the uterus into a collecting tube. All four goats had catheter inserted through the cervix and uteri flushed successfully. The volume (recovery rate) of recovered medium varied considerably from 87 ml/200 ml (43.5%) to 148 ml/160 ml (92.5%). Nine embryos/ova in total were recovered from Gravity-flow group goats. Although the embryo recovery rate was low, the possibility of a transcervical embryo recovery in Korean black goat had been proven in this preliminary experiment.

Performance and Hydraulic Characteristics of Drip Emitters (점적 emitter 의 성능과 수리적 특성)

  • 이남호
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.41 no.3
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    • pp.33-40
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    • 1999
  • Variations in the discharge rates of drip emittes were examined to find the effects of operation pressure and the tube length and to evaluate performance of the emitters. Several point-source emitters were selected such as pressure compensated, anti-leak pressure compensated, turbulent flow regulated, flow regulated, ready-made dripper, and spaghetti. Combination of operation pressure and tube length were compared. The microirrigatioon system was operated at pressures of 0.5 , 1.0 , 1.5 and 2.0 bar. The discharge from emitters wer collected at every ten meters along the lateral tube and weighted. In order to evaluate the drip emitters performance coeffcient of discharge variation , statistical uniformity, and emission uniformity were calculated. No significant variation in discharge along drip tube resulted with all emitters. There is no trend of variatiiono of discharge rate from pressure compensated emitters with increase in operation pressures. But discharge rate from other types of emitters increased with increase in operation pressures. The nominal discharge of each emitter was secured at pressure of 1.0 bar, Evaluation using statiscal and emission uniformity coefficients indicated that most of the emitters excepts tubulent flow regulated emitter and ready-made dripper performed at excellent level.

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A Streamfiow Network Model for Daily Water Supply and Demands on Small Watershed (II) - Model Development - (중소유역의 일별 용수수급해석을 위한 하천망모형의 개발(II) -모형의 구성-)

  • 허유만;박창언;박승우
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.35 no.2
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    • pp.23-32
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    • 1993
  • This paper describes the background and the development of a hydrologic network flow model. The model was development to simulate daily water demand and supply for selected stream reaches within a watershed, and used as a tool for evaluating, simulating, and planning a water resources system. The proposed network flow model considers daily runoff from subareas, various water demands, and diversion structures within each subarea. Daily streamflow at a reach is simulated after balancing the water demands from subareas. The lateral inflow from subareas is simulated using a modified tank model. Total water demands consist of the daily demands for agricultural, domestic, industrial, livestock, fishery, and environmental uses within a rural district. The return flow, diversions from sources and storage components such as reservoirs were also incorporated into the mode l . The developed model is a generalized version that may be applied to different combinations of river reaches for a given system. This may help potential users identify areas where water supply does not suffice the demands for different time horizons.

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Performance Analysis of Multiple Wave Energy Converters due to Rotor Spacing

  • Poguluri, Sunny Kumar;Kim, Dongeun;Ko, Haeng Sik;Bae, Yoon Hyeok
    • Journal of Ocean Engineering and Technology
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    • v.35 no.3
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    • pp.229-237
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    • 2021
  • A numerical hydrodynamic performance analysis of the pitch-type multibody wave energy converter (WEC) is carried out based on both linear potential flow theory and computational fluid dynamics (CFD) in the unidirectional wave condition. In the present study, Salter's duck (rotor) is chosen for the analysis. The basic concept of the WEC rotor, which nods when the pressure-induced motions are in phase, is that it converts the kinetic and potential energies of the wave into rotational mechanical energy with the proper power-take-off system. This energy is converted to useful electric energy. The analysis is carried out using three WEC rotors. A multibody analysis using linear potential flow theory is performed using WAMIT (three-dimensional diffraction/radiation potential analysis program), and a CFD analysis is performed by placing three WEC rotors in a numerical wave tank. In particular, the spacing between the three rotors is set to 0.8, 1, and 1.2 times the rotor width, and the hydrodynamic interaction between adjacent rotors is checked. Finally, it is confirmed that the dynamic performance of the rotors slightly changes, but the difference due to the spacing is not noticeable. In addition, the CFD analysis shows a lateral flow phenomenon that cannot be confirmed by linear potential theory, and it is confirmed that the CFD analysis is necessary for the motion analysis of the rotor.

The Evaluation of Lateral Scatter Ray of Gamma Camera (Gamma Camera에 있어 측면 선란선의 영향에 대한 평가)

  • Kim, Jae-Il;Lee, Eun-Byeol;Cho, Seong-Wook;Noh, Kyeong-Woon;Kang, Keon-Wook
    • The Korean Journal of Nuclear Medicine Technology
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    • v.22 no.1
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    • pp.46-50
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    • 2018
  • Purpose Generally, a collimator that installed in front of detector set a direction of gamma ray and remove a scatter ray. By the way, a lateral or oblique scatter ray is detected into crystal through collimator. At this study, we will evaluate a mount of count and spectrums of lateral scatter ray. Materials and Methods We used the SKY LITE (philips, netherlands) as a gamma camera, and $^{99m}Tc$, 1.11 GBq point source as a phantom. we put this point source at backside 50 cm of detector. After acquiring this for 1 min, we turned a detector next 10 degrees. Likely this, we acquired images at every 10 degrees from $0^{\circ}$ to $360^{\circ}$, analyzed images and spectrums. In case of patient study, we choose a 3 phase bone scan patient who had a hand disease, because scatter rays from body would detect on crystal. After acquiring blood flow and blood pool images, we analyzed images and spectrums. Additional, we put a lead gown on patient's hand, body. And then we compared and evaluated 3 type blood pool images (non lead gown, lead gown on a hand and on body). Results In case of phantom study, scatter ray counts at backside ($270^{\circ}-90^{\circ}$) are same with a background count. By the way, counts of scatter ray of oblique side ($0^{\circ}-50^{\circ}$, $220^{\circ}-270^{\circ}$) are 100-600 cps, furthermore, counts at frontside are over 4 Mcps. In case of patient study, a counts of hand blood pool scan are 1510 cps. But counts of hand with lead gown on hands and on body are each 1554 cps, 1299 cps. Conclusion Therefore, even though there is a collimator in front of detector, lateral scatter rays detect on crystal and affect to images and spectrums. Especially, if there is a high activity source at outside of detector when we examine low activity organs like hands or foot, we have to shield and remove the source at outside for a good image.

Development of Lateral Flow Immunofluorescence Assay Applicable to Lung Cancer (폐암 진단에 적용 가능한 측면 유동 면역 형광 분석법 개발)

  • Supianto, Mulya;Lim, Jungmin;Lee, Hye Jin
    • Applied Chemistry for Engineering
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    • v.33 no.2
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    • pp.173-178
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    • 2022
  • A lateral flow immunoassay (LFIA) method using carbon nanodot@silica as a signaling material was developed for analyzing the concentration of retinol-binding protein 4 (RBP4), one of the lung cancer biomarkers. Instead of antibodies mainly used as bioreceptors in nitrocellulose membranes in LFIA for protein detection, aptamers that are more economical, easy to store for a long time, and have strong affinities toward specific target proteins were used. A 5' terminal of biotin-modified aptamer specific to RBP4 was first reacted with neutravidin followed by spraying the mixture on the membrane in order to immobilize the aptamer in a porous membrane by the strong binding affinity between biotin and neutravidin. Carbon nanodot@silica nanoparticles with blue fluorescent signal covalently conjugated to the RBP4 antibody, and RBP4 were injected in a lateral flow manner on to the surface bound aptamer to form a sandwich complex. Surfactant concentrations, ionic strength, and additional blocking reagents were added to the running buffer solution to optimize the fluorescent signal off from the sandwich complex which was correlated to the concentration of RBP4. A 10 mM Tris (pH 7.4) running buffer containing 150 mM NaCl and 0.05% Tween-20 with 0.6 M ethanolamine as a blocking agent showed the optimum assay condition for carbon nanodot@silica-based LFIA. The results indicate that an aptamer, more economical and easier to store for a long time can be used as an alternative immobilizing probe for antibody in a LFIA device which can be used as a point-of-care diagnosis kit for lung cancer diseases.

Microdevice for Separation of Circulating Tumor Cells Using Embedded Magnetophoresis with V-shaped Ni-Co Nanowires and Immuno-nanomagnetic Beads

  • Park, Jeong Won;Lee, Nae-Rym;Cho, Sung Mok;Jung, Moon Youn;Ihm, Chunhwa;Lee, Dae-Sik
    • ETRI Journal
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    • v.37 no.2
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    • pp.233-240
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    • 2015
  • The novelty of this study resides in a 6"-wafer-level microfabrication protocol for a microdevice with a fluidic control system for the separation of circulating tumor cells (CTCs) from human whole blood cells. The microdevice utilizes a lateral magnetophoresis method based on immunomagnetic nanobeads with anti-epithelial cell adhesive molecule antibodies that selectively bind to epithelial cancer cells. The device consists of a top polydimethylsiloxane substrate for microfluidic control and a bottom substrate for lateral magnetophoretic force generation with embedded v-shaped soft magnetic microwires. The microdevice can isolate about 93% of the spiked cancer cells (MCF-7, a breast cancer cell line) at a flow rate of 40/100 mL/min with respect to a whole human blood/buffer solution. For all isolation, it takes only 10 min to process 400 mL of whole human blood. The fabrication method is sufficiently simple and easy, allowing the microdevice to be a mass-producible clinical tool for cancer diagnosis, prognosis, and personalized medicine.

The Settlement Behavior Analysis of SCP of Multi-Layered Ground in Incheon (인천지역 다층지반에 시공된 SCP의 침하거동 분석)

  • Yoon, Won-Sub;Kim, Jong-Kook;Park, Sang-Jun;Cho, Chul-Hyun;Chae, Young-Su
    • Proceedings of the Korean Geotechical Society Conference
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    • 2008.03a
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    • pp.1042-1050
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    • 2008
  • In this study, SCP method was used by purpose to improve loose sand and soft clay that is drilled Sand Compaction Pile in underground. Settlement behavior of field analyzed through SCP method. When sand Compaction Pile drilled in clay, forming composite ground that foundation and Sand Compaction Pile behavior. According to SCP method can expect bearing capacity improvement, Settlement reduction, lateral flow protection. SCP increase the consolidation settlement of ground and it reduce settlement for that purpose increase liquefaction resistance, lateral Resistance. Because SCP had been widely used for sand. Area of Inchon-A by sand compose clay and silt to upper Ground and compose soft clay to under ground. After pre-loading, it measured settlement by extensometer and settlement extensometer that purpose of ground improvement with 13% in replacement ratio. The result analyzed settlement behavior is similar to Multi-layered Ground that it happened to elastic settlement at upper ground and to consolidation settlement at under ground.

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