Insects constitute the largest and most diverse group of animals in the world. They also serve as the hosts or nutrient sources for an immense assemblage of pathogens, parasites, and predators. More than 700 fungal species from 100 genera have adopted an entomopathogenic lifestyle. Although entomopathogenic fungi were studied as only biocontrol agents against a variety of pests in various countries, it has been recently focused their additional roles in nature. They are antagonists to/against plant pathogens, endophytes, and possibly even plant growth promoting agents. The potential antimicrobial effect against fungal plant pathogens by an isolate of entomopathogenic fungi including Beauveria bassiana, Lecanicillium spp., and Isaria fumosorosea have been reported since late 1990s, but wasn't reported pathogenicity of the isolate against pests. Later, a Canadian Lecanicillium sp. isolate and L. longisporium isolated from Vertalec$^{(R)}$ showed simultaneous control effect against both aphid and cucumber powder mildew. Therefore, the antimicrobial activities of 342 fungi isolates collected from various regions and conditions in Korea were evaluated against plant pathogenic fungus Botrytis cinerea using dual culture technique on agar plate. As a result, 186 isolates (54.4%) shown the antifungal activity against B. cinerea. The culture filtrates of selected fungi completely suppressed the growth of the microorganisms, indicating that suppression was due to the presence of antimicrobial substances in the culture filtrate. Mode of action of these fungi against insect involves the attachment of conidia to the insect cuticle, followed by germination, cuticle penetration, and internal dissemination throughout the insect. During infection process, secreted enzymes, proteinous toxins, and/or secondary metabolites secreted by entomopathogenic fungi can be used to overcome the host immune system, modify host behavior, and defend host resources. Recently, secondary metabolites isolated from entomopathogenic fungi have been reported as potential bioactive substances. Generally, most of bioactive substances produced by entomopathogenic fungi have reported low molecular weight (lower than 1,000 g/mol) as peptide and, in contrast the high molecular weight fungal bioactive substances are rare. Most substances based on entomopathogenic fungi were shown antimicrobial activity with narrow control ranges. In our study we analyzed the antimicrobial substances having antagonistic effects to B. cinerea. Antimicrobial substances in our fungal culture filtrates showed high thermostability, high stability to proteolytic enzymes, and hydrophilicity and their molecular weights were differed from substance. In conclusion, entomopathogenic fungi showed pathogenicity against insect pests and culture filtrate of the fungi also shown to antimicrobial activity. In the future, we can use the entomopathogenic fungi and its secondary metabolites to control both insect pest control and plant pathogenic fungi simultaneously.
Lee, Kyung Joon;Sohn, Jae Hyung;Redei, K.;Yun, Hye Young
Journal of Korean Society of Forest Science
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v.96
no.2
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pp.170-177
/
2007
The objectives of this study were to select early, late, and abundant flowering trees of black locust from domesticated and introduced cultivars, and prediction of flowering period by calculation of accumulated temperature in spring. Four cultivars (Debreceni-2, Pusztavacs, Jaszkiseri, and Rozsaszin AC) from Hungary and a cultivar from Beijing, China, were introduced, propagated by seed and planted in a seed orchard. For domesticated black locust, 63 cultivars from 10 locations throughout the country were selected and propagated by root cutting. Criteria for selection of domesticated cultivars were abundant flowering, long flowering period, or abundant nectar production with, if possible, straight stems. Accumulated temperature was calculated from data of a nearby weather station by accumulating daily maximum temperature minus 5 degree Celsius from January 1 up to the date reaching 880 degrees. Daily mean temperature was also used to calculate accumulated temperature up to the date reaching 450 degrees. The percentages of two-year and three-year-old flowering trees propagated by root cutting were higher than that of trees propagated by seeds, while four-year-old trees all flowered regardless of propagation methods. Among the domesticated cultivars, all the cultivars from Ganghwa showed abundant flowering with highest nectar production of 6.5 ul per flower, which was 100% more than other domesticated cultivars and 50% more than Debreceni-2 cultivar with highest nectar production among the introduced cultivars from Hungary. At the end of the eight years of observations, two trees of Debreceni-2 cultivars and a tree from Beijing, China were selected for early flowering trees which flowered 2 to 3 days earlier than average trees, while a tree of Debeceni-2 and three trees from Bejing were selected for late flowering trees which flowered 2 to 3 days later than average trees. It is possible to extend the flowering period of black locust by 4 to 6 days by planting early and late flowering cultivars together. Abundant flowering trees were unable to be selected due to severe damages by leaf gall midges which killed many trees and reduced the crown size of the remaining trees in the seed orchard, and which were first found in Korea in 2001 and now damaging most of the black locust forests in Korea. The prediction of flowering period by accumulated temperature indicated that black locust flowered to a peak when accumulated daily maximum temperature reached 880 degrees Celsius, and when daily mean temperature reached 450 degrees.
Despite of ecological and landscape importances and public popularity of Pinaceae, not much scientific informations are known about Korean Pinaceae. Present work aims to understand the biogeography of Korean native Pinaceae, i.e., taxonomy, phylogeny, origin, life form, distribution, dispersal and migration. Korean native Pinaceae consists of five genera and sixteen species. Pinus is systematically closely related to Picea and Larix, but Abies is related to Tsuga. Boreal conifers which have migrated from NE Asia during the Pleistocene glacial epochs successfully survived, but now confined to the alpine and subalpine belts of the Korean Peninsula mainly due to climate warming since the Holocene. Species, such as Picea pungsanensis and Abies koreana have gradually adapted to local environment, and later became an endemic species of Korea. Disjunctive distribution of Pinus parviflora and Tsuga sieboldii are also indicatives of climate change of the Pleistocene. Major dispersal agent of pine trees with winged seed is wind, but wingless pine tree seeds seem to dispersed by birds and rodents. Pine trees with bigger wings are easily dispersed by wind, and now show broader distribution. Species of Pinaceae with disjunctive distribution on the alpine and subalpine belts of both North and South Korea seems to be more vulnerable to global warming.
Christina Rossetti's novella Maude displays Tractarian influences in terms of Holy Eucharist, Puseyism, and the doctrine of Reserve. Tractarianism is High Church revival movement of nineteenth century. In the story a teenage girl, Maude went through hard time receiving Holy Eucharist due to self-consciousness and internal guilt according to Puseyism. She felt guilty when she enjoyed worldly things and outward beauty. Due to guilt Maude refused to receive Holy Communion, which is complete connection to God. Her cousin, Agnes suggested that in refusing Holy Communion Maude is following her own will not God's will. Later Maude overcame Puseyite thought of self-hatred and reconciled with her identity as a poet and a woman. Maude oscillates between concealing and revealing, secrecy and truth, sincerity and affectation, and modesty and display. Her marvelous poetic talent makes people praise her but she withholds private feelings and attempts to divert attention from herself. Like Maude herself, the meaning of her poems is at times reserved and withheld. This tendency goes with the doctrine of Reserve in Tractarianism. The doctrine of Reserve utilizes indirect methods to reveal divine attributes because finite human being can not accept infinite God. The doctrine of Reserve sees to it that the expression will be veiled, indirect, subdued and self-effacing. Rossetti adapts a poetic method of Reserve when Maude has anxiety over 'display and poetry' and generates the reticence, secrecy, mystery, renunciation, modesty and detachment. According to Mary Arseneau, by veiling and expressing herself through symbols she can rise above the self and employ the phenomenal to suggest a noumenal reality. Thus the poetry becomes an expression of longing for the divine. The poem "Three Nuns" exemplifies Maude's maturity and gradual progress in the relationship with God. Rossetti suggests the vision full of hopes and promises of reuniting with God. In conclusion, in some sense, authoritative and conservative Tractarianism affects Rossetti both ways. On the one hand, it makes Rossetti abnegate herself and leads her to asceticism, on the other hand, it makes Rossetti express her faith in God and write amazing devotional poems such as "Three Nuns". A poem within the poem has three voices that are in perfect harmony. In the poem the first and second nun show hesitation to fully commit to God's will and the desire for the world prevents them from having heavenly joy. Third nun reveals spiritual maturity and sings new life in God where their hopes and joys begin. Rossetti expresses the procedure of spiritual growth through the poem "Three Nuns". For Rossetti, self-abnegation and self-expression both are involved in the doctrine of Reserve, Puseyism and Holy Communion.
This study was conducted to improve commercial quality of a cut spray chrysanthemum 'Ilweol' bred in Korea by foliar application of daminozide, suppressing excessive elongation of peduncle caused by high temperature in summer season production. Applications were made at three floral bud developmental stage (I, II, and III), and concentrations used were 0, 500, 1000, and $2000mg{\cdot}L^{-1}$. Cut flower length and peduncle length showed the greatest repression of elongation through daminozide treatments at stage III and stage II, respectively. Daminozide also gradually reduced values of those in a concentration-dependant manner. Flower diameter increased compared to control as daminozide was sprayed at later stage but decreased in daminozide concentration-dependant manner. Angle of flower cluster on apical part of whole plant showed the best results when sprayed with $2,000mg{\cdot}L^{-1}$ at stage III. Among all combinations, $2,000mg{\cdot}L^{-1}$ daminozide at stage II recorded the highest angle of flower cluster and showed 31.3% increment as compared to control. The number of fully expanded flowers showed the highest values using $2,000mg{\cdot}L^{-1}$ daminozide at stage III than the other treatments and total floral buds also showed the highest values using $2,000mg{\cdot}L^{-1}$ daminozide at stage I than the other treatments. In cut spray chrysanthemum cultivar 'Ilweol' bred in Korea, foliar-applying with $2,000mg{\cdot}L^{-1}$ at stage III most effectively suppressed excessive peduncle elongation, angle of flower cluster, and increased the number of flowers in summer. However, this combination affected negatively the other commercial qualities, reducing cut flower length and flower diameter. Therefore, we recommended that foliar application stage and daminozide concentration was stage III and $500mg{\cdot}L^{-1}$, respectively, in a practical culture and cut flower quality for 'Ilweol'.
The purpose of this study was to identify frailty profiles based on physical, psychological, and social domains of functioning and to examine the associated factors showing the differences among frailty profiles. Respondents were 70 years and older(n=403) and latent class analysis was applied to determine the optimal subgroups based on Tilberg Frailty Indicators which comprised of three domains(the physical, psychological, and social domain). Also, we performed multinominal logistic regression analysis to find out factors making differences among frailty profiles. Latent class analysis(LCA) identified three distinct types: multi-frail type(27.0%), psychologically frail type(26.8%), inadequate support type(46.2%). All three types had common difficulties in dealing with daily life problems and did not receive enough help with theses difficulties. Based on the results of the LCA three-class models, people in multi-frail type accumulated problems in physical and psychological domains and had partially social domain. On the other hands, psychologically frail type showed a relatively high anxiety disorder and depression. Lastly, people in inadequate support type reported the lack of helps, but they were relatively healthy. Comparing these groups with inadequate support type, people with multi-frail had lower educational level, poor nutritional management status and were less likely to participate in labor market. People in psychologically frail type were more likely to be male, to live in big cities rather than middle and small cities, and less likely to smoke. Based on these results, our results showed the multifaceted concept of frailty among Korean elderly people and we suggested several implications for preventing frail process.
The Journal of Korean society of community based occupational therapy
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v.9
no.1
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pp.57-67
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2019
Objective : This study aimed to identify types of leisure time spending of the Korean elderly according to the data of Hours of Living 2014 from National Statistical Office, and compare and analyze features of leisure time spending based on general characteristics of the elderly. Method : This study has only adopted the data of 9228 elder people whose ages were above 65, and their general characteristics and time spending features form the data of Hours of Living 2014. The final comparison and analysis has been made through behavior classification (1 major classification, 7 medium classification, 39 minor classification), which reflects how the elderly spend their leisure time according to their household and personal characteristics. Result : Firstly, the Korean elderly were found to spend most of their leisure time with media. Secondly, analyzing the features of leisure time spending based on household characteristics of the elderly, their gender and household income showed significant differences in all types of leisure time activities except for cultural and sightseeing activity. And their marital status showed significant differences in most of the leisure time activities excluding leisure time activity with media and cultural and sightseeing activity. Thirdly, analyzing the features of leisure time spending based on personal characteristics of the elderly, their education level has made significant differences in all types of leisure time activities. As for their economic activity status and average monthly income, they showed meaningful differences in most of the leisure time activities excluding cultural and sightseeing activity and ritual activity. Conclusion : We should help to find meaningful occupation within personal contexts and arrange it properly with balanced leisure time spending. Moreover, it is highly important to have appropriate occupational approach to prevent social isolation of the Korean elderly, and ensure their fruitful and successful later life.
Cho, Hyoung Sun;Kwon, Yonghwan;Kim, Young-Ung;Kang, Jin-Su;Lee, Kichang;Kim, Namsoo;Kim, Min Su
Journal of Veterinary Clinics
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v.36
no.1
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pp.53-61
/
2019
Total hip arthroplasty (THA) is a successful surgical treatment for both patients with chronical lameness and dogs who are nonresponsive to medical treatments, providing excellent joint function for returning dogs to the normal gait in 80% to 98% of hip dysplasia (HD) patients. The THA surgical implant system manufactured by BioMedtrix and Kyon are today widely accepted. When comparing the BioMedtrix biological fixation (BFX) system to the BioMedtrix cemented fixation (CFX) system, the many advantages of BFX, which include longer potential implant life, decreased risk of postoperative or later infection, and better implant stability, become evident. However, BFX implies a greater risk of femoral fracture during reaming and requires a more precise surgical technique to achieve good implant fit, given the press-fit nature of cementless THA. The purposes of this study are to both describe the mistakes and complications during stem implantation for beginner surgeons with both the BFX and the CFX systems and to document the initial result of 12 implantations in canine cadavers. Given the detailed evaluations of 3 specialists, who are Diplomate American College of Veterinary Surgeons (DACVS), only 3 of 11 stems were appropriately sized. Specifically, 6 stems were anteverted rather than being retroverted; further, although 7 stems were coaxial with the femoral long axis in the frontal plane, the other stems were in the varus at the frontal plane, with the proximal medial stem adjacent to the medial femoral cortex. Moderate angulation from the cranial to the caudal directions was found in 4 cases in the sagittal plane. Additionally, 1 case of femoral fissure and 1 case of perforated femoral cortex were reported. It is not easy for surgeons performing cementless THA for the first time to achieve a good result, even though they completed an educational course about it and given that catastrophic complications often occurred during early surgical clinical cases. Therefore, ex-vivo studies are sincerely required to get an expertise by rehearsing the preparation of the femoral envelop in isolated bones. Further studies should be conducted to achieve both highly accurate implant size and correct orientation during the preoperative planning. Additionally, surgeons' learning curve should be examined in future investigations.
Hyowon6, a mid-late maturing, good eating quality rice variety with multi-resistance, was developed by the rice breeding team of Pusan National University in 2013. This variety was derived from a cross between Hwayeong (as a multi-resistant parent) and Koshihikari (as a good eating quality parent). Selection by the pedigree breeding method was carried out until the $F_8$ generation. A promising line, JS14-12-36-8-5-3-1-1-1, was developed and designated as HY103 in 2013. This variety headed on August 22, which was 11 days later than that of Hwayeong. The culm length and panicle length of Hyowon6 was 85.7 cm and 21.1 cm, respectively. The number of panicles per hill was 14.5 and the number of grains per panicle was 101.7. The ratio of ripened grain was approximately 92% and the 1,000-grain weight was approximately 21.36 g for brown rice, which were similar to those of Hwayeong. Hyowon6 was moderately resistant to lodging and also to neck blast, leaf blight, and stripe virus. The glossiness value of Hyowon6 was 83, which was considerably higher than that of Hwayeong.
Long-term observation of the life cycle of plants allows the identification of critical signals of the effects of climate change on plants. Indeed, plant phenology is the simplest approach to detect climate change. Observation of seasonal changes in plants using digital repeat imaging helps in overcoming the limitations of both traditional methods and satellite remote sensing. In this study, we demonstrate the utility of camera-based repeat digital imaging in this context. We observed the biological events of plants and quantified their phenophases in the northern temperate type deciduous broadleaf forest of Jeombong Mountain. This study aimed to identify trends in seasonal characteristics of Quercus mongolica (deciduous broadleaf forest) and Pinus densiflora (evergreen coniferous forest). The vegetation index, green chromatic coordinate (GCC), was calculated from the RGB channel image data. The magnitude of the GCC amplitude was smaller in the evergreen coniferous forest than in the deciduous forest. The slope of the GCC (increased in spring and decreased in autumn) was moderate in the evergreen coniferous forest compared with that in the deciduous forest. In the pine forest, the beginning of growth occurred earlier than that in the red oak forest, whereas the end of growth was later. Verification of the accuracy of the phenophases showed high accuracy with root-mean-square error (RMSE) values of 0.008 (region of interest [ROI]1) and 0.006 (ROI3). These results reflect the tendency of the GCC trajectory in a northern temperate type deciduous broadleaf forest. Based on the results, we propose that repeat imaging using digital cameras will be useful for the observation of phenophases.
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