• 한국광학회:학술대회논문집
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• 한국광학회 2008년도 동계학술발표회 논문집
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• pp.453-454
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• 2008

femtosecond laser를 광원으로 하는 optical tweezers는 광포획 뿐만 아니라 비선형 현상을 발생시킬 수 있다는 장점을 가지고 있다. 그러나 높은 첨두 출력에 의하여 포획된 세포는 쉽게 손상되어 질 수 있다. 본 논문에서는 LTRS(Laser Tweezers Raman Spectroscopy)를 통하여 femtosecond laser와 CW laser에 의한 optical tweezers 상에서의 optical damage를 비교, 분석하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.1501-1506
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• 2005

This paper presents the design of a multiple beam optical tweezers instrument used for manipulating micro/nano-sized components. The basic equations used in designing the optical tweezers are derived and the stable and time-sharing multiple beam optical tweezers are constructed with scanning mirrors. The laser beam passes through a series of optical components such as lenses, mirrors, and scanning mirrors, and overfills the entrance aperture of microscope objective, which gives a stable trap. By rotating the laser beam with the scanning mirror, the focal positions are translated in the specimen plane and multiple micro/nano-sized objects can be moved. The constructed optical tweezers is used to manipulate cells and liposomes simultaneously and to trap multiple nano-wires. The experiments prove that the developed optical tweezers can be a very versatile manipulation tool for studying gene therapy and nano device fabrication.

• 한국CDE학회논문집
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• 제12권4호
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• pp.298-307
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• 2007

In recent years, researchers are actively investigating new methods that are applicable for manufacturing micrometer to nanometer scale structures. Among them, optical tweezers that can manipulate microscopic objects using a laser is receiving one of the key attentions. Optical tweezers have been used actively in the field of science. For example, for measuring mechanical characteristics in the scale of piconewtons or for manipulating and sorting large numbers of particles, bacteria, cells. etc. However, little works have been reported for "manufacturing" objects. In this paper, we present a new method for manufacturing micrometer scale structures using micrometer scale biotin coated polystyrene particles. Particles will be controlled with a user interface that utilizes a CyberGlove and glued together by the bonding force between biotin and streptavidin.

• 한국광학회지
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• 제19권2호
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• pp.132-139
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• 2008

주사 레이저 광집게에서 대물렌즈에 의해 집속된 레이저 초점을 제어하기 위해 사용되는 고속 구동기는 입력 신호의 주파수가 증가함에 따라 출력이 감소한다. 이러한 입출력의 괴리는 CCD 카메라를 통해 관찰이 어려우며 사용자는 희미하게 보이는 레이저 주사 형상을 보고 물체를 조작하여 물체를 포획할 수 없거나 포획하더라도 안정된 제어를 할 수 없다. 본 연구에서는 이러한 문제를 해결하기 위해 사용된 고속 구동기의 주파수 특성을 분석하고, 이를 바탕으로 입력 주파수에 따른 실제 주사 경로를 측정하여 시각화해주는 방법과 입출력 데이터의 차이를 계산하여 입력 데이터를 보상하는 방법을 제안한다.

• 대한기계학회논문집B
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• 제29권1호
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• pp.110-115
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• 2005

A novel technique for fine particle beam focusing under the atmospheric pressure is introduced using a radiation pressure assisted aerodynamic lens. To introduce the radiation pressure in the aerodynamic focusing system, a 25m plano-convex lens having 2.5mm hole at its center is used as an orifice. The particle beam width is measured for various laser power, particle size, and flow velocity. In addition, the effect of the laser characteristics on the beam focusing is evaluated comparing an optical tweezers type and pure gradient force type. For the pure aerodynamic focusing system, the particle beam width was decreased as increasing particle size and Reynolds number. Using the optical tweezers type, the particle beam width becomes smaller than that of the pure aerodynamic focusing system about $16\%,\;11.4\%\;and\;9.6\%$ for PSL particle size of $2.5{\mu}m,\;1.0{\mu}m,\;and\;0.5{\mu}m$, respectively. Particle beam width was minimized around the laser power of 0.2W. However, as increasing the laser power higher than 0.4W, the particle beam width was increased a little and it approached almost a constant value which is still smaller than that of the pure aerodynamic focusing system. For pure gradient force type, the reduction of the particle beam width was smaller than optical tweezers type but proportional to laser power. The radiation pressure effect on the particle beam width is intensified as Reynolds number decreases or particle size increases relatively.

• 한국광학회지
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• 제19권1호
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• pp.80-83
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• 2008

광집게는 매질보다 큰 굴절률을 가지는 마이크로 크기의 구형 유전체를 강하게 집속되는 레이저를 이용해서 포획하고 움직이는 도구이다. 본 논문에서는 FDTD 방법을 이용해서 포획 힘을 계산하고, 그 방법을 설명하였다. 강하게 집속되는 레이저는 nonparaxial Gaussian beam을 이용해서 표현하였으며, 레이저가 대상물체와 매질에서 진행하는 것은 FDTD 방법을 이용해서 시뮬레이션 하였다. 레이저를 계산공간 전체에서 해석적으로 표현하기 위해서 scattered field formulation을 이용하였다. FDTD 방법을 이용해서 대상물체의 안팎의 전자기장을 시뮬레이션하고, 그 결과를 이용해서 Maxwell's stress tensor에 기반하여 포획 힘을 계산하였다.

• 한국광학회:학술대회논문집
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• 한국광학회 2008년도 동계학술발표회 논문집
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• pp.237-238
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• 2008

• Journal of the Optical Society of Korea
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• 제11권4호
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• pp.162-172
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• 2007

Scanning Laser Optical Tweezers(SLOT) is an optical instrument frequently employed on a microscope with laser being delivered through its various ports. In most SLOT systems, a mechanical tilt stage with a mirror on top is used to dynamically move the laser focal point in two-dimensions. The focal point acts as a tweezing spot, trapping nearby microscopic objects. By adding a mechanical translational stage with a lens, SLOT can be expanded to work in three-dimensions. When two mechanical stages operate together, the focal point can address a closed three-dimensional volume that we call a workspace. It would be advantageous to have a large workspace since it means one can trap and work on multiple objects without interruptions, such as translating the microscope stage. However, previous studies have paid less consideration of the volumetric size of the workspace. In this paper, we propose a new method for designing a SLOT such that its workspace is maximized through optimization. The proposed method utilizes a matrix based ray tracing method and genetic algorithm(GA). To demonstrate the performance of the proposed method, experimental results are shown.

• Journal of the Optical Society of Korea
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• 제20권1호
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• pp.78-87
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• 2016

Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC’s membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.402.1-402.1
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• 2014

Photonic force microscopy (PFM) is an optical tweezers-based scanning probe microscopy, which measures the forces in the range of fN to pN. The low stiffness leads proper to measure single molecular interaction. We introduce a novel photonic force microscopy to stably map various chemical properties as well as topographic information, utilizing weak molecular bond between probe and object's surface. First, we installed stable optical tweezers instrument, where an IR laser with 1064 nm wavelength was used as trapping source to reduce damage to biological sample. To manipulate trapped material, electric driven two-axis mirrors were used for x, y directional probe scanning and a piezo stage for z directional probe scanning. For resolution test, probe scans with vertical direction repeatedly at the same lateral position, where the vertical resolution is ~25 nm. To obtain the topography of surface which is etched glass, trapped bead scans 3-dimensionally and measures the contact position in each cycle. To acquire the chemical mapping, we design the DNA oligonucleotide pairs combining as a zipping structure, where one is attached at the surface of bead and other is arranged on surface. We measured the rupture force of molecular bonding to investigate chemical properties on the surface with various loading rate. We expect this system can realize a high-resolution multi-functional imaging technique able to acquire topographic map of objects and to distinguish difference of chemical properties between these objects simultaneously.