• 대한치과교정학회지
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• 제29권2호
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• pp.165-181
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• 1999

교정치료시 치아와 주위조직의 부작용을 최소로 하면서 최대의 치료결과를 얻기 위해서는 치료역학을 각 환자의 치아 및 주위 해부학적 환경에 맞도록 개인화 시켜야한다. 특히 성인 교정시 문제되기 쉬운 치근흡수 또는 치주질환으로 인한 치조골 손실로 인하여 치관/치근 비율이 변했을 때 치아의 저항중심위치의 변화와 관련된 생역학적 반응의 차이에 주의하여야 한다. 본 연구에서는 정상 치주조직뿐만 아니라 다양한 비정상적 치주 및 치아상태에서 치관/치근 비율이 변하였을 때 일정한 교정력하에서의 치아의 초기이동 양상을 연구하기 위하여, 성인의 인체 건조 두개골 및 하악골상에서 laser 반사측정법 및 lever and pulley force applicator와 photodetector를 이용하여, 상악 6전치군을 대상으로는 치조골높이를 각각의 치아에 대하여 2mm씩 총 8mm까지 감소시켰고, 하악 6전치군을 대상으로는 치근길이를 각각의 치아에 대하여 2mm씩 총6mm까지 감소시키면서, 이렇게 다양하게 정량화된 변수들 하에서 상하악6전치군의 저항중심의 위치변화를 연구한 결과 다음과 같은 결론을 얻었다. 1. 초기상태에서 상악 6전치군의 초기이동시의 저항중심점의 위치는 6전치 전체 평균치아 치근의 치경부(CEJ)로부터 치근첨 방향으로 약 $42.4\%$되는 위치에 있었으며, 각 치아의 치조골의 높이가 감소할수록 저항중심점은 치근첨방향으로 약 $76.7\%$되는 부위까지 이동하였다. 2. 상악 6전치군에서 저항중심점의 6전치 전체 평균 치조정으로부터의 거리는 치조골의 감소와 함께 지속적으로 감소하였으나, 치조골내의 평균 치근의 길이에 대한 비율은 치조골의 감소에 상관없이 약 $33\%$내외에서 비교적 일정하였다. 3. 초기상태에서 하악 6전치군의 초기이동시의 저항중심점의 위치는 6전치 전체 평균치아 치근의 CEJ로부터 치근첨방향으로 약 $43\%$되는 위치에 있었으며, 각 치아의 치근의 길이가 감소할수록 이 비율은 약 $54\%$까지 증가하였다. 그러나 CEJ로부터 저항중심점까지의 거리는 5.3mm전후로부터 3.3mm내외까지 감소하여 치근의 길이가 감소할수록 저항중심점이 CEJ방향으로 이동하였다. 4.치조골 또는 치근흡수시, 각각의 단위 흡수량에 따른 저항중심위치의 변화에 미치는 영향은 초기에는 치조골이 감소 될 때가 더 컸으나 전체 평균 치근길이의 중간부위에서의 영향은 비슷했다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권6호
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• pp.360-367
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• 2013

Purpose: The purpose of this study was to perform three-dimensional (3D) assessment of facial soft tissue in patients with skeletal Class III and mandibular asymmetry after orthognathic surgery. Methods: Samples consisted of 3D facial images obtained from five patients with A point-nasion-B point angle less than 2 degrees, and more than 5 mm of menton deviation. All patients had been treated at Gangneung-Wonju National University Dental Hospital from 2009 to 2012. They had undergone orthognathic surgery of Lefort I, and sagittal split osteotomy for correction of skeletal deformity, and orthodontic treatment. Facial scanning was performed before treatment (T1) and post-surgical orthodontic treatment (T2). Linear and angle variables of soft tissue landmarks, antero-posterior facial depth, and facial volume were measured. Results: No significant differences in width of the alar base, mouth width, and nasal canting were observed between T1 and T2. However, lip deviation, menton deviation, alar canting, lip canting, and menton deviation angle were significantly reduced at T2. Antero-posterior facial depth on the axial plane parallel to the left cheilion was significantly reduced on the deviated side and significantly increased on the non-deviated side at T2. Volume of the lower lateral and lower medial parts of the face was reduced on the deviated side, and volume of upper lateral and lower lateral parts on the non-deviated side was significantly increased at T2. Conclusion: After orthognathic surgery, facial asymmetry of soft tissue was improved following skeletal changes, especially the mandibular region. Although the length of the alar base and mouth width did not change, lip and soft tissue menton were displaced to the medial side after treatment. Facial depth also became symmetric after treatment. Facial volume showed a decrease on the lower part of the deviated side and that on lateral parts of the non-deviated side showed an increase after treatment.

• 대한치과교정학회지
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• 제36권1호
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• pp.45-54
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• 2006

최근 안모의 심미성이 중요시되면서 구순 돌출로 인한 비심미성을 해소하고자 내원하는 양악 치조골 전돌자의 수가 증가하는 추세이다. 그러나 이러한 환자에 대한 연구는 많지 않으며 특히 모형을 이용한 연구는 극히 적다. 모형을 이용하는 연구는 주로 손으로 직접 계측하거나 2차원 평면에서 컴퓨터를 이용하여 측정하였으나 근래에 3차원 레이저 스캐너가 도입되면서 모형을 이용한 연구를 좀 더 빠르고 간편하면서도 정확하게 할 수 있게 되었다. 이 연구는 3차원 디지털 모형을 이용하여 양악 치조골 전돌자의 치열궁 특성을 알아보기 위한 것이다. 양악 치조골 전돌자 20명과 정상교합자 20명을 대상으로 치아 크기, 치열궁 폭경 및 치열궁 길이, 치열궁과 구개의 형태를 비교하여 본 결과 양악 치조골 전돌자는 정상교합자보다 치아 크기가 크고, 하악 견치간 폭경 및 제1소구치간 폭경이 크며 치열궁 길이가 길었다. 그리고 치열궁 형태와 구개 형태에서 정상교합자와 차이를 보였다.

• Journal of Periodontal and Implant Science
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• 제47권2호
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• pp.116-131
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• 2017

Purpose: The entry of bacteria or harmful substances through the epithelial seal of human gingival keratinocytes (HGKs) in the junctional epithelium (JE) is blocked by specialized intercellular junctions such as E-cadherin junctions (ECJs). However, the influence of roughened substrates, which may occur due to apical migration of the JE, root planing, or peri-implantitis, on the development of the ECJs of HGKs remains largely unknown. Methods: HGKs were cultured on substrates with varying levels of roughness, which were prepared by rubbing hydrophobic polystyrene dishes with silicon carbide papers. The activity of c-Jun N-terminal kinase (JNK) was inhibited with SP600125 or by transfection with JNK short hairpin RNA. The development of intercellular junctions was analyzed using scanning electron microscopy or confocal laser scanning microscopy after immunohistochemical staining of the cells for E-cadherin. The expression level of phospho-JNK was assessed by immunoblotting. Results: HGKs developed tight intercellular junctions devoid of wide intercellular gaps on smooth substrates and on rough substrates with low-nanometer dimensions (average roughness $[Ra]=121.3{\pm}13.4nm$), although the ECJs of HGKs on rough substrates with low-nanometer dimensions developed later than those of HGKs on smooth substrates. In contrast, HGKs developed short intercellular junctions with wide intercellular gaps on rough substrates with mid- or high-nanometer dimensions ($Ra=505.3{\pm}115.3nm$, $867.0{\pm}168.6nm$). Notably, the stability of the ECJs was low on the rough substrates, as demonstrated by the rapid destruction of the cell junction following calcium depletion. Inhibition of JNK activity promoted ECJ development in HGKs. JNK was closely associated with cortical actin in the regulation of ECJs in HGKs. Conclusions: These results indicate that on rough substrates with nanometer dimensions, the ECJs of HGKs develop slowly or defectively, and that this effect can be reversed by inhibiting JNK.

• Journal of Periodontal and Implant Science
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• 제44권2호
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• pp.79-84
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• 2014

Purpose: While single-species biofilms have been studied extensively, we know notably little regarding multispecies biofilms and their interactions. The purpose of this study was to develop and evaluate an in vitro multispecies dental biofilm model that aimed to mimic the environment of chronic periodontitis. Methods: Streptococcus gordonii KN1, Fusobacterium nucleatum ATCC23726, Aggregatibacter actinomycetemcomitans ATCC33384, and Porphyromonas gingivalis ATCC33277 were used for this experiment. The biofilms were grown on 12-well plates with a round glass slip (12 mm in diameter) with a supply of fresh medium. Four different single-species biofilms and multispecies biofilms with the four bacterial strains listed above were prepared. The biofilms were examined with a confocal laser scanning microscope (CLSM) and scanning electron microscopy (SEM). The minimum inhibitory concentrations (MIC) for four different planktonic single-species and multispecies bacteria were determined. The MICs of doxycycline and chlorhexidine for four different single-species biofilms and a multispecies biofilm were also determined. Results: The CLSM and SEM examination revealed that the growth pattern of the multispecies biofilm was similar to those of single-species biofilms. However, the multispecies biofilm became thicker than the single-species biofilms, and networks between bacteria were formed. The MICs of doxycycline and chlorhexidine were higher in the biofilm state than in the planktonic bacteria. The MIC of doxycycline for the multispecies biofilm was higher than were those for the single-species biofilms of P. gingivalis, F. nucleatum, or A. actinomycetemcomitans. The MIC of chlorhexidine for the multispecies biofilm was higher than were those for the single-species biofilms of P. gingivalis or F. nucleatum. Conclusions: To mimic the natural dental biofilm, a multispecies biofilm composed of four bacterial species was grown. The 24-hour multispecies biofilm may be useful as a laboratory dental biofilm model system.

• Journal of Periodontal and Implant Science
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• 제30권2호
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• pp.471-482
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• 2000

Recent studies have demonstrated that smoking may be one of the most significant risk factors in the development and progression of periodontal disease. Reports have indicated that smoking causes gingival blood flow to be decreased. However, studies on the effects of smoking on gingival blood flow have yielded contradictory results. The purpose of the present study was to determine the effect of smoking on gingival blood flow. One hundred volunteers(fifty non-smokers and fifty smokers) with good general and periodontal health, aged twenties(non-smoker : 22-29 years, mean=25.36, smoker : 23-29 years, mean=26.64) were selected. Laser Doppler flowmetry (floLAB, Moor Instruments Ltd., England) was applied to measure the gingival blood flow of interdental papilla, marginal gingiva, attached gingiva and alveolar mucosa of left and right upper lateral incisors. In smokers, following an overnight abstinence from smoking, gingival blood flow was measured before smoking, immediately after smoking, 1-, 2-, 3-, 4-, 5- and 6- hour after smoking from 9 a.m. to 3:30 p.m. The difference of blood flow in each tissue of non-smokers and that of each measuring time and each tissue of smokers were statistically analyzed by one way ANOVA and Tukey test. And the difference of blood flow between smokers and nonsmokers in each tissue was statistically analyzed by t-test. The results were as follows : 1. Mean blood flow was highest in alveolar mucosa, followed by interdental papilla, attached gingiva and marginal gingiva in both smokers and nonsmokers. There was a statistically significant difference in each tissue(p<0.05) . 2. There was no consistent result between mean blood flow before smoking in smokers and that of nonsmokers in each tissue. 3. There was a statistically significant difference between gingival blood flow at measuring time point and gingival blood flow of smokers in each tissue(p<0.05). The present study suggested that smoking could alter the gingival blood flow, thus might be partly contributed to periodontal destruction.

• Journal of Periodontal and Implant Science
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• 제43권2호
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• pp.72-78
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• 2013

Purpose: The purpose of this study was to compare the phototoxic effects of blue light exposure on periodontal pathogens in both planktonic and biofilm cultures. Methods: Strains of Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Porphyromonas gingivalis, in planktonic or biofilm states, were exposed to visible light at wavelengths of 400.520 nm. A quartz-tungsten-halogen lamp at a power density of $500mW/cm^2$ was used for the light source. Each sample was exposed to 15, 30, 60, 90, or 120 seconds of each bacterial strain in the planktonic or biofilm state. Confocal scanning laser microscopy (CSLM) was used to observe the distribution of live/dead bacterial cells in biofilms. After light exposure, the bacterial killing rates were calculated from colony forming unit (CFU) counts. Results: CLSM images that were obtained from biofilms showed a mixture of dead and live bacterial cells extending to a depth of $30-45{\mu}m$. Obvious differences in the live-to-dead bacterial cell ratio were found in P. gingivalis biofilm according to light exposure time. In the planktonic state, almost all bacteria were killed with 60 seconds of light exposure to F. nucleatum (99.1%) and with 15 seconds to P. gingivalis (100%). In the biofilm state, however, only the CFU of P. gingivalis demonstrated a decreasing tendency with increasing light exposure time, and there was a lower efficacy of phototoxicity to P. gingivalis as biofilm than in the planktonic state. Conclusions: Blue light exposure using a dental halogen curing unit is effective in reducing periodontal pathogens in the planktonic state. It is recommended that an adjunctive exogenous photosensitizer be used and that pathogens be exposed to visible light for clinical antimicrobial periodontal therapy.

• 대한치과기공학회지
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• 제24권1호
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• pp.73-81
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• 2002

Accurate acquisition of surface geometries such as machined surfaces, biological surfaces, and deformed parts have been very important technique in scientific study and engineering, expecially for system design, manufacturing and inspection. Two camera method is relatively simple with an acceptable accuracy. In this paper, a new method is studied to acquire 3D geometric data of the small object such as a die in stone model. When the devices, cameras, laser beam and object are in a perfect plane, the calculation becomes very simple with less error. But this paper shows that arbitrarily positioned system can also be used to obtain 3D data.

• 대한치과기공학회지
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• 제35권4호
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• pp.281-286
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• 2013

Purpose: Surface modification is important techniques in modern dental and orthopedic implants. This study was performed to try embedding of bioactive materials in porous Ti implants. Methods: Porous Ti implant samples were fabricated by sintering of spherical Ti powders in a high vacuum furnace. It's diameter and height were 4mm and 20mm. Embedding process was used to suction and vacuum chamber. Loading properties of porous Ti implants were evaluated by scanning electron microscope(SEM), confocal laser scanning microscope(CLSM), and UV-Vis-NIR spectrophotometer. Results: Internal pore structure was formed fully open pore. Average pore size and porosity were $10.253{\mu}m$ and 17.506%. Conclusion: Porous Ti implant was fabricated successfully by sintering method. Particles are necking strongly each other and others portions were vacancy. This porous structure can be embedded to bioactive materials. Therefore bioactive materials will be able to embedding to porous Ti implants. Bioactive materials embedding in the porous Ti implant will induced new bone faster.

• 대한소아치과학회지
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• 제32권1호
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• pp.89-100
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• 2005

형태학적으로 아주 유사한 상, 하악 제2유구치와 제1대구치의 교합면 형태를 분석하고자 정상교합의 유치열기 아동(Hellman dental age II A) 100명(남자 50명, 여자 50명)과 영구치열기의 성인(Hellman dental age IV A) 86명 (남자 43명, 여자 43명)을 대상으로 하였다. 상, 하악 제2유구치와 제 1대구치의 상, 하악 석고모형의 3차원 형상 data로부터 각 교두정간 거리, 교두정을 최소한의 오차로 지나는 평면과 교합면사이의 체적, 평면에서 교두정간 까지의 방향별 Section curve를 구하여 다음과 같은 결론을 얻었다. 1. 형성된 표준평면과 각 교두정과의 거리에 관한 오차는 하악 제2유구치에서 남자 0.05-0.09mm, 여자 0.04-0.09mm로서 제일 작았다. 2. 각 교두정간의 거리는 하악 제2유구치와 제 1대구치 에서 남자가 크게 나타났다. 특히 제2 유구치에서는 유의성이 존재하였다(p<0.05). 3. 남녀 모두에서 사주 교두거리를 제외하고, 상악 제2유구치는 원심 협측교두와 설측교두 사이가, 하악 제2유구치는 원심교두와 원심 설측교두 사이가, 상악 제1대구치는 근심 설측교두와 협측교두 사이가, 하악 제1대구치는 원심 설측교두와 근심 설측교두 사이의 거리가 가장 크게 측정되었다. 4. 제2유구치와 제1대구치에서 교합면 체적은 하악에서 크게 나타났고, 영구치가 1.40-1.75배 값을 보였으며(p<0.05), 남녀간에는 남자가 큰 값을 보이긴 하였지만 통계적인 유의성이 없었다. 5. 대부분의 경우 유치열에서 보다 영구치열에서 section curve가 넓고 깊었으며 교두사이의 사선거리를 제외하고 상악의 경우 근심 협측과 설측교두 사이에서 유치열과 영구치열 모두에서 가장 깊은 section curve를 이루었으며 하악에서는 영구치열은 원심 협측과 원심교두사이 유치열은 원심 설측과 원심교두 사이에서 가장 깊은 section curve를 이루었다.