• Bulletin of the Korean Chemical Society
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• 제34권6호
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• pp.1668-1672
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• 2013

We developed a standoff Raman detection system for explosive molecules (EMs). Our system was composed of reflective telescope with 310 mm diameter lens, 532 nm pulse laser, and Intensified Charge-Coupled Device (ICCD) camera. In order to remove huge background noise coming from ambient light, laser pulses with nanosecond time width were fired to target sample and ICCD was gated to open only during the time when the scattered Raman signal from the sample arrived at ICCD camera. We performed standoff experiments with military EMs by putting the detector at 10, 20 and 30 m away from the source. The standoff results were compared with the confocal Raman results. Based on our standoff experiments, we were able to observe the peaks in the range of 1200 and $1600cm^{-1}$, where vibrational modes of nitro groups were appeared. The wave numbers and shapes of these peaks may serve as good references in detecting and identifying various EMs.

• 한국기계가공학회지
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• 제12권6호
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• pp.23-29
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• 2013

In this paper, pure $I_{ph}$ and hybrid carbon nanotube reinforced $I_{ph}$ were sintered using the SPS(spark plasma sintering) method for high densification. A nanosecond laser (${\lambda}=1063nm$, ${\tau}P=10ns$) and a femtosecond laser (${\lambda}=1027nm$, ${\tau}P=380fs$) were installed on an optical system for the micromachining test. The ablation characteristics of the pure $I_{ph}$ and CNT/$I_{ph}$ composites, such as thermal effect and ablation depth, were investigated using FE-SEM and a confocal microscope device. Laser machining results for the two mating materials showed improved performances: CNT/$I_{ph}$ composites showed good surface morphology of hole drilling without a melting zone due to the composites' high thermal properties; also, the ablated depth of CNT/$I_{ph}$ was higher than that of pure $I_{ph}$.

• 한국가시화정보학회지
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• 제22권2호
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• pp.74-81
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• 2024

Accurate tissue temperature monitoring during clinical procedures, such as laser therapy or surgery, is crucial for ensuring patient safety and treatment efficacy. Noninvasive techniques are essential to prevent tissue disturbance while providing real-time temperature data. However, current methods often struggle to accurately measure temperature at various depths within the skin, which is essential to avoid damage to surrounding healthy tissues due to excessive heat. In response to this challenge, we developed a confocal imaging system that utilizes the laser speckle imaging (LSI) technique for precise depthwise temperature monitoring. LSI uses laser light scattering to capture subtle changes in speckle patterns on the skin's surface due to temperature fluctuations within the tissue. By analyzing these changes, LSI enables accurate depth-resolved temperature measurements. This technique enhances the precision and safety of medical procedures, offering significant potential for broader clinical applications, improved patient outcomes, and better thermal management during interventions.

• 대한소아치과학회지
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• 제34권2호
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• pp.183-191
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• 2007

본 연구는 Digital Imaging Fiber-Optic Trans-Illumination(DIFOTI)이나 Laser Fluorescence(LF)를 이용한 방법이 평활면 법랑질 초기우식증의 재광화 정도를 정확하게 monitoring 할 수 있는지의 여부를 평가하기 위하여 진행되었다. 우치 법랑질로부터 얻어진 인공 우식 절편을 특별히 제작된 의치에 식립한 다음, 10명의 실험 참가자들이 구강 내에 장착하고 3주 동안 불소 양치용액을 사용하도록 한 다음 Confocal Laser Scanning Microscopy(CLSM)로 측정된 병소 깊이를 gold standard로 사용하여 DIFOTI와 LF로 측정 된 우식 절편의 재광화 정도를 비교 평가한 후 다음과 같은 결론을 얻었다. 1. DIFOTI에 측정된 광도 백분율이 재광화 기간이 지남에 따라 증가하였으며, CLSM에서 측정된 병소 깊이와 유의한 역상관관계를 보였다(r=-0.683, p<0.01). 2. LF 측정치는 재광화 기간이 경과함에 따라 증가하였으며 CLSM에서 측정된 병소 깊이와 유의한 상관관계를 보였다(p<0.05). 3. CLSM 촬영 결과 500 ppm 불소 양치군이 0 ppm 불소 양치군보다 빠른 속도로 병소 깊이가 감소하는 양상을 보여 주었다.

• IMMUNE NETWORK
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• 제1권3호
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• pp.187-195
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• 2001

The expression of recombinant proteins fused to 26 kDa glutathione S-transferase (GST) extracted from Schistosoma japonicum represents an attractive system for purifiying proteins of interest in a single step using GST-affinity chromatography. In addition, the GST-tag is used conveniently for detecting fused proteins since its high solubility as well as its relatively small size rarely interferes with the biological activity of the fused protein. In this regard, the GST system is frequently applied for tracing fusion proteins in both prokaryotic and eukaryotic cells to elucidate the physiological interactions and functional compartments of proteins. To provide a further tool in analyzing GST-fusion proteins, a new monoclonal antibody, with a high specificity to the S. japonicum GST was produced. Methods: BALB/c mice were immunized both with recombinant S. japonicum GST proteins, and by the fusion of splenocytes from these mice with myeloma cells. From this, a new anti -GST monoclonal antibody, termed SARAH, was generated. The specificity and reactivity of this antibody was confirmed by ELISA and by Western blot analysis. Results: SARAH showed a high reactivity to recombinant GST and GST fusion protein but not with native mammalian GST proteins as derived from other species including humans, cows, rabbits and rats. The applicability of SARAH was further demonstrated by confocal laser scanning microscopy, where GST proteins that were expressed transiently in mouse fibroblast cells, were specifically detected without interference of endogenous GST. Conclusion: SARAH is new monoclonal antibody with a high specificity to recombinant GST proteins but not to endogenous GST in mammalian cells.

• 한국광학회지
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• 제17권3호
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• pp.273-277
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• 2006

홀버닝 광메모리의 광원으로서 상용 반도체 레이저 다이오드에 Littman형 및 고정방향 Littrow형 외부공진기를 결합하여 파장가변 레이저 다이오드 시스템을 제작하고 성능을 비교 분석하였다. 두 형태의 파장가변 레이저 다이오드 시스템 모두 0차 출력광은 단일종모드로 동작하며 CFP(Confocal Fabry-Perot)의 분해능인 9MHz이내의 선폭을 예상할 수 있었으며 다이오드 구동전류 140mA 및 동작 온도 $25^{\circ}C$의 조건하에서 거울 및 회절격자를 수동나사로 회전시키는 성긴 튜닝시 Littman형은 5.375nm, 고정방향 Littrow형은 13.65 nm이상의 파장가변 범위를 보였고 PZT (Piezoelectric Transducer)에 200Hz의 톱니파 전압을 인가해서 거울을 회전시키는 미세 튜닝 시 두 형태 모두 0.042nm의 범위 내에서 연속적인 파장가변이 가능하였다. 특히 고정방향 Littrow형 외부공진기는 출력광의 방향이 바뀌는 기존의 단점을 보강하였으며, 또한 외부공진기의 길이에 따른 최대파장가변 범위를 측정하였다.

• 한국광학회지
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• 제5권3호
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• pp.423-430
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• 1994

DFB-LD 칩으로부터 단일보드 광섬유 부착 2.5Gbps 광통신용 광원인 DFB-LD 모듈을 설계, 제작하였다. DFB-LD 모듈은 광 isolator가 삽입된 2 렌즈 quasi confocal 광학계로 구성된 원통형 서브 모듈과 14 pin butterfly 패키지가 분리된 구성으로서 이들 사이의 전기적 연결은 bias-T 회로가 형성된 하이브리드 기판으로 이루어지도록 설계하였다. 모듈 제작시 정밀한 부품 고정이 요구되는 서브 모듈 조립에는 레이저웰딩 방법을 사용하였다. 제작된 DFB-LD 모듈은 광결합 효율 20%, -3dB 소신호 변조 대역폭 2.6GHz 이상의 특성을 가졌으며 온도 순환검사에도 10% 이내의 광출력 변동만을 보임으로써 기계적 신뢰성을 확인할 수 있었다. 제작된 DFB-LD 모듈의 광송신 성능을 실제 2.5Gbps 광통신 시스템의 광원으로 적용하여 평가한 결과 47km의 광섬유 전송시 BER $1\times10^{-10}$ 조건에서 최대 -30.2dBm의 수신감도를 얻었으며 이 때 전송페널티는 소광비에 의한 것이 1.5dB, 분산에 의한 것이 1.0dB로 나타났다.

• Restorative Dentistry and Endodontics
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• 제43권4호
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• pp.46.1-46.10
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• 2018

Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of $50{\mu}m$ inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of $50{\mu}m$ in the dentinal tubules.

• 광물과 암석
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• 제35권3호
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• pp.299-311
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• 2022

지표에 노출된 암석은 지속적으로 풍화를 받게 되고 이러한 영향으로 암석의 공학적 안정성이 약해지게 된다. 특히 풍화가 진행되면서 암석의 표면은 풍화에 의해서 변화를 일으키고 이러한 표면 변화는 암석으로 구성된 지반의 공학적 안전성에 영향을 미치게 된다. 또한, 풍화를 받은 암석에서 생성되는 화학종은 주변환경에 직접적인 영향을 미치거나 구조물에 영향을 미치게 된다. 광산지역과 같이 암석이 노출된 지역에서는 풍화에 의해 생성된 화학종이 주변 자연환경에 심각한 영향을 미치기도 한다. 본 연구에서는 이러한 관점에서 동결/융해 실험을 활용한 풍화가속 실험을 이미 풍화를 받은 암석과 신선한 암석을 대상으로 실시하고 각 암석의 표면 변화를 다초점 레이저 현미경으로 관찰하고 IC/ICP-MS를 활용하여 화학종 생성에 대한 분석을 실시하였다. 풍화가 진행됨에 따라 표면의 거칠기는 완화되는 것을 확인하였고 주변환경에 영향을 미칠 수 있는 화학종은 풍화를 받는동안 양이 증가하는 것을 확인하였다. 본 연구 결과는 암석이 노출된 지역에서의 공학적/환경학적 안전성을 평가하는 기초 자료로 활용될 수 있을 것이다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.49-55
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• 2008

Strip bending test has been frequently utilized to measure the mechanical properties of freestanding thin films in substitute for the micro-tensile test. However, in spite of its simplicity and reliability, strip bending test has a few problems, for example, the measurement of strain and the calculation of stress at zero strain. In this study, these problems are precisely reviewed and proved. Upon this review, strip bending tester has been developed, which uses the confocal laser displacement meter to measure the deformed configuration of the specimen and the possibility and limitation of this testing system is carefully investigated including the estimation of uncertainty of the measurement of strain. Finally, to prevent errors and to improve the accuracy of this testing system, the shape of the specimen has been carefully studied and is proposed.