• Parasites, Hosts and Diseases
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• v.51 no.6
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• pp.633-636
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• 2013

Angiostrongylus cantonensis is a parasitic nematode that needs to develop in different hosts in different larval stages. Freshwater snails, such as Pomacea canaliculata, are the intermediate host, and rats are the definitive host. Periodic shedding of the cuticle (moulting) is an important biological process for the survival and development of the parasite in the intermediate and definitive hosts. However, there are few studies on the cuticle alterations between different stages of this parasite. In this study, we observed the ultrastructural appearance and changes of the cuticle of the 2nd/3rd stage larvae (L2/L3) and the 3rd/4th stage larvae (L3/L4) using a scanning electron microscope. We also first divided L2/L3 into late L2 and early L3. The late L2 lacked alae, but possessed a pull-chain-like fissure. Irregular alignment of spherical particles on the cuticle were noted compared to the L3. Alae appeared in the early L3. The old cuticle turned into a thin filmlike structure which adhered to the new cuticle, and spherical particles were seen regularly arranged on the surface of this structure. Regular rectangular cavities were found on the surface of L3/L4. The caudal structure of L3/L4 was much larger than that of L3, but caudal inflation, such as seen in L4, was not observed. These results are the first to reveal the ultrastructural changes of the cuticle of A. cantonensis before and after moulting of L2/L3 and L3/L4.

• Journal of Sericultural and Entomological Science
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• v.53 no.1
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• pp.1-5
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• 2015

As increasing utilization of Tenebrio molitor for industry, establishing effective conditions of indoor mass-rearing system become more important. For this reason, the aim of this study was to identify the physiological characteristics of Tenebrio molitor in different range of temperatures, photoperiods and rearing densities for mass breeding. As a result, their egg had above 70% hatching rate at $17.5{\sim}27.5^{\circ}C$ and the egg period was shown 5~7 days in $25{\sim}35^{\circ}C$. Consequently, in the consideration of the hatching rates and egg periods, the optimal indoor temperature for rearing was $25{\sim}27.5^{\circ}C$. Furthermore, development period of larvae in mass breeding system was shorter than individual breeding system under all the conditions of temperature. Also, the pupal weight was higher in mass breeding system except for $20^{\circ}C$ and $25^{\circ}C$. Considering elasticity of demand and weight of production, the effective condition was mass-breeding system at $25^{\circ}C$. In photoperiodic condition, the shortest of larval period was 157.35 day in 14L : 10D.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.337-343
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• 2011

In order to know the effects of rearing conditions such as water temperature, salinity, larval stocking density, kinds and amounts of food organism and seedling collection method on seedling production of the brackish water clam, Corbicula japonica, the growth and survival rate of the larvae were investigated at each rearing condition. The shell length of larvae showed faster growth at $24^{\circ}C$ and $27^{\circ}C$, and survival rate showed good results at $18^{\circ}C$ and $21^{\circ}C$. Based on growth and survival rate according to water temperature, the optimal water temperature for larvae rearing was $24^{\circ}C$. At the salinity of more than 3 psu, the growth and survival rate were higher than 0 psu. The optimal salinities for larvae were 6-9 psu. Growth and survival rate of larvae were high at low stocking density and the optimum stocking density of larvae was 10 inds./ml. Daily feeding concentrations of 10,000-20,000 cell/ml of food organisms mixed Pavolva lutheri, Isochrysis galbana and Chaetoceros calcitrans were very effective. Full grown larvae collection from various substratum was significantly higt in sand, especially sand size was less than 0.25 mm.

• Environmental Analysis Health and Toxicology
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• v.24 no.2
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• pp.119-125
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• 2009

This study was carried out to investigate the toxic effect of WSF (water soluble fraction) in crude oil on the hatch, survival rate, abnormality and physiological activity of fertilized eggs and early larvae in Paralicthys olivaceus. The time required in hatching the fertilized eggs exposure to crude oil was 50.8${\sim}$53.2 hours both in control group and experimental group, showing no significant difference(p>0.05). The hatching rate in the control group was more than 80% in 1.9 ${\mu}g/mL$ WSF concentration, but hatching rate was less than 55.7% in below of 3.9 ${\mu}g/mL$ WSF concentration, showing significant difference (p<0.05). The survival rate on the 3rd day of early larval stage was 61.96% with 1.9 ${\mu}g/mL$ WSF, 11.1% with 3.9 ${\mu}g/mL$ WSF, and they all died with other concentration levels. The oxygen consumption rate in experimental groups was lower than control group. The heart rate (no./min) was 47.4${\sim}$52.8 before hatching and there was no significant difference between control group and experimental group, but heart rate reduced with the decrease of WSF concentration after hatching. The abnormality was 1.1% in the control group whereas 36.7% with 3.9${\mu}g/mL$ WSF, and abnormality was mostly comprised of incomplete spinal formation and spinal curvature. The results of this study suggest that even low concentration to WSF affects the early development of the flounder, Paralicthys olivaceus.

• Journal of Aquaculture
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• v.17 no.3
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• pp.221-227
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• 2004

In the present study, we investigated the effects of food and salinity on growth and survival of river puffer, Takifugu obscurus offsprings in indoor land-based tank during the early development. In the food experiment, the river puffer larvae (TL 8.7$\pm$0.1 mm, BW 20.0$\pm$3.2 mg) were fed with tubificid, Limnodrilus gotoi, water flea, Daphnia carinata, mysid, Neomysis awatschensis, Manila clam, Ruditapes philippinarum and artificial food together Artemia nauplii for 30 days, and the growth and the survival of larvae were investigated. In the salinity experiment, the river puffer larvae and juvenile at three stages (Stage I: TL 5.8$\pm$0.4 mm; Stage II: TL 12.0$\pm$0.2 mm, Stage III： TL 44.5$\pm$0.7 mm) were supplied with Artemia nauplii, water flea and/or artificial food and were reared in 0, 10, 20 and 30 psu at respective stages. The results shows that the growth rates of larvae feeding with artificial food and tubificid together Artemia nauplii were higher than those of larvae in other groups, but the survival rates of larvae feeding with water flea and mysid together Artemia nauplii were higher than those of larvae in other groups. In salinity, the results shows that the growth and survival rates of offsprings were significantly higher in 10∼20 psu than those in 0 and 30 psu at Stage I and n. At Stage III, the growth and the survival rates were the highest in 20 psu, but the lowest in 0 psu. However, it should be noted that the rates in 0 and 30 psu were significantly enhanced at Stage III in comparison with those at Stage I and II. Therefore, it is concluded that river puffer, T. obscurus is euryhaline marine species, which can normally grow and live at salinities from 0 to 30 psu, and that a combinative supplement with Artemia nauplii and water flea, D. carinata may confer an advantage on growth and survival of the river puffer offsprings in indoor land-based tank.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.1-7
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• 2003

Morphological characteristics, developmental period, and seasonal occurrence of Adeia leucomelas (L.) were investigated from 1999 to 2000. In addition, consumption of sweetpotato as food was also examined. Adults of A. leucomelas were dark-brown and body lengths of females and males were 20.2 mm and 18.9 mm, respectively, Wing expanse of female and male was 33.7 mm and 29.4mm, respectively. Egg was flat round-shape. Larva was light yellow-green to dark-brown with 3.3-53.5 mm. Pupa was deep-brown and 15.1 mm in length. Developmental periods of A. leucomelas from egg to adult emergence at different temperatures of 15, 20, 25, and 30$^{\circ}$C were 108.5, 70.7, 40.2, and 29.1 days, respectively, Developmental threshold (DT) and effective accumulative temperatures were estimated as 10.7$^{\circ}$C and 67.5 DD in egg stage, 11.0$^{\circ}$C and 275.1 DD in larval stage and 9.3$^{\circ}$C and 244.6 DD in pupal stage, respectively. The longevity of adult female was shortened with increment of temperature, whereas the total numbers of eggs laid by a female were increased. The larvae of A.leucomelas occurred from mid-June to early October, and population reached its peak during early to mid-September in Jeonbuk province. Food consumption of A. leucomelas was highest at 20-25$^{\circ}$C. Food consumption of 1 st, 2nd, 3rd, 4th, and 5th larvae of A. leucomelas per day at 25$^{\circ}$C was 0.4, 3.6, 19.6, 40.7, and 78.9 $\textrm{cm}^2$, respectively.

• Korean journal of applied entomology
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• v.57 no.3
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• pp.165-175
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• 2018

Insects are known to live at wide range of temperature, but can not survive when they are exposed to over $40^{\circ}C$ or below supercooling point. The larvae of Helicoverpa assulta have been reared at high ($35^{\circ}C$), low (3 to $10^{\circ}C$), and room temperature ($25^{\circ}C$; control). To identify stress-related genes, the transcriptomes of fat body have been analyzed. Genes such as cuticular proteins, fatty acyl ${\Delta}9$ desaturase and glycerol 3 phosphate dehydrogenase were up-regulated whereas chitin synthase, catalase, and UDP-glycosyltransferase were down-regulated at low temperature. Superoxide dismutase, metallothionein 2, phosphoenolpyruvate carboxykinase and trehalose transporter have been up-regulated at high temperature. In addition, expressions of heat shock protein and glutathione peroxidase were increased at high temperature, but decreased at low temperature. These temperature-specific expressed genes can be available as markers for climate change of insect pests.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.2
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• pp.86-93
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• 1981

For daily supply of rotifers to feed juvenile fish and/or larval crustaceans such as shrimp in their seedling culture procedures, the maximum daily increase of cultured rotifers in relations with the inoculation densities of rotifers and the concentrations of food organism, Chlorella sp. were initially investigated in an experimental culture unit. The best results were obtained from the 10 inds./ml of the rotifer inoculation density with $3\times10^6\;cells/ml$ of Chlorella showing the maximum daily increase of 73.0 inds./ml/day on the 7th day. Based on the best results of the initial experiments a trial was made for continuous daily harvests of rotifers. As in the initial experiments, the maximum daily increase of rotifer was observed on the 7th day, when the daily harvest was initiated and continued once a day for 6days. For the 6 days, the amount of the mean daily harvest was $1.42\times10^5$ individuals per culture flask ($3{\iota}\;filled\;with\;2{\iota}$culture water) shelving a possibility of continuous daily harvests.

• Ocean and Polar Research
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• v.26 no.3
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• pp.425-431
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• 2004

The study focuses on the morphology of larval and juvenile codlets, Bregmaceros atlanticus, collected from the East China Sea in May 2001 and September 2002. A total of 47 individuals were collected by using an IKMT, and the range of their body lengths was $2.41{\sim}17.18$ mm. Specimens can be categorized into two groups: larvae and juvenile. The first group, in which the yolk is completely absorbed, had a body length of 2.41m. They had big heads, and large and thick melanophores were sparsely scattered about the head, bottom of the pectoral fins, rear parts of the trunk and ends of the notochords. The specimens had a body length of 2.54mm and over, and thin and elongated body trunks. Their heads were significantly smaller, including the body depth, and melanophores were coarsely developed all over their bodies. Occipital rays, the first and unique-ray dorsal fin, were observed on individuals of body length greater than 4.01 m, and the rays grew proportionally with body length, but didn't reach the root of the second dorsal fin. The second group, juveniles, had an body length of 9.82mm and over, and their fin rays satisfied the completed numbers of rays and vertebrae for adults. Melanophores were densely scattered all over the body, where thick colors were observed on the rear parts of the dorsal and anal fins and front part of the caudal fin.

• Korean Journal of Ichthyology
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• v.10 no.2
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• pp.207-215
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• 1998

Just after hatching the embryo has a yolk sac and straighted digestive tract. Just after parturition in 5.0mm TL the mouth and anus were opened. In the larval stage of 5.2mm TL, digestive tract could be divided into pharynx, esophagus, stomach, pyloric caeca and intestine of the early differentiated state. The esophagus of larvae in 5.3mm TL has a PAS positive mucous cell. From over 5.7mm TL, PAS positive goblet cell recognized in the intestine. PAS positive absorptive cell began to appear in the intestine from 5.9mm TL. Yolky materials were absorbed completely in 6.0mm TL. In the larvae of B.5mm TL, gastric glands were observed in the mucosa, but the surface epithelium did not have PAS positive granules. From over 9.0mm TL, the histological structure of esophagus showed similar to adult. In the juvenile stage from 13.0mm to 15.0mm TL, histological structure of the stomach, pyloric caeca and intestine showed similar to adult. From the ultrastructural and histochemical study, it is concluded that the functional digestive tract is present in the juvenile stage from 18.0mm to 20.0mm TL.