• International Journal of Industrial Entomology and Biomaterials
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• v.19 no.2
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• pp.221-228
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• 2009

Nosema sp. infection in the Indian tasar silkworm, Antheraea mylitta exerts a complex of influences on its host. The instar duration was extended significantly (P<0.001) except in $1^{st}$ instar. The infected larvae took about 48 days to reach the spinning stage against 40 days in the uninfected ones. The final weight attained by the larva at the end of each instar of development declined significantly following infection, as did weight gain and relative growth rate (RGR). The growth recorded/ day declined in infected larvae compared to uninfected ones from 8.2% during $1^{st}$ instar to 43.3% during $5^{th}$ instar. Food ingestion and digestion increased with advancement of the instar significantly irrespective of the status of the larvae but the relative consumption rate (RCR) declined. These parameters significantly declined in infected larvae (except food digested during $2^{nd}$ instar). The decline was more during $3^{rd}$ instar. In contrast, the approximate digestibility (AD %) was significantly higher in infected larvae than uninfected ones leaving the $1^{st}$ instar larvae unaffected. The efficiency of conversion of ingested food (ECI) and efficiency of conversion of digested food (ECD) did not change in a patterned way following the microsporidia (Nosema sp.) infection. The values of ECI significantly changed during $2^{nd}$, $3^{rd}$ and $5^{th}$ instars; while the change in ECD during $2^{nd}$, $4^{th}$ and $5^{th}$ were significant. During the entire larval life all the parameters tends to decline significantly following microsporidia infection but AD registered a significant increase. Nosema sp. spore concentration has increased 270.7 times during larval development in the course of experimentation.

• BMB Reports
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• v.36 no.3
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• pp.294-298
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• 2003

The pathological effect of the Bacillus thuringiensis Cry $\delta$-endotoxins on susceptible insect larvae had extensive damage on the midgut epithelial cells. In this study, an ex vivo assay was devised for assessing the insecticidal potency of the cloned Cry4B mosquito-larvicidal protein that is expressed in Escherichia coli. Determination of toxicity was carried out by using a cell viability assay on the midguts that were dissected from 5-day old Aedes aegypti mosquito larvae. After incubation with the toxin proteins, the number of viable epithelial cells was determined photometrically by monitoring the quantity of the bioreduced formazan product at 490 nm. The results showed that the 65-kDa trypsin-activated Cry4B toxin exhibited toxic potency ca. 3.5 times higher than the 130-kDa Cry4B protoxin. However, the trypsin-treated products of the non-bioactive Cry4B mutant (R158A) and the lepidopteran-specific Cry1Aa toxin displayed relatively no ex vivo activity on the mosquito-larval midguts. The ex vivo cytotoxicity studies presented here confirms data that was obtained in bioassays.

• The Korean Journal of Ecology
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• v.14 no.4
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• pp.449-455
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• 1991

The water striders, gerris paludum insularis larvae which were fed with the drosophila melanogaster cultured on the appli diet and artificial diet, were reared in the growth cabinet controlled as the two constant temperature regimes of 25 c and 30 c under the condition of photoperiod 16l : 8d, light intensity 510 240 lux, relative humidity 65 3%, and in the natural state. The effects of temperature and diet on the devolopment of g. paludum insularis were analyzed and the results were summarized as follows. 1. The total developmental period of the water strider larvae fed with the d. melanogaster reared on the apple diet at the natural stste was the longest 40 days, and the total devopmental periods at 25 c and 30 c were 35 days and 27 days respectively. The tolal developmental period of larvae fed with d. melanogaster reared on the artifical diet at 25 c was the longest 42 dats, and the total developmental periods at natural stste and 30 c were 32 days and 27 days, respectively. 2. In the larval developmental stages, the fifth stage was longer than that of any stage. The duration of egg stage which was not influenced by diet at 25 c was the longest. 3. The water strider larvae fed which the d. melanogaster reared on the artificial diet at 25 c did not complete their devolopment. 4. No significant difference was odserved in the developmentperiod for diet at 30 c. but, there were significant differences in developmental periods for diet at 25 c and natural state. 5. The rate of adult emergence on the apple diet was higher than that of the artificial diet was higher than of the artificial diet. 6. The shapes of the survivorship curve were convex-curve. 7. The development periods of the larval stages of water striders might be affected to temperature and diet dependently.

• Parasites, Hosts and Diseases
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• v.45 no.1 s.141
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• pp.19-26
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• 2007

This study describes the isolation of a Toxocara canis species-specific excretory-secretory(ES) antigen and the development of an enzyme-linked immunosorbent assay(ELISA) based on this antigen. Analysis of the ES antigens of T. canis, Toxocara vitulorum, Ascaris lumbricoides and Necator americanus larval antigen was performed by SDS-PAGE followed by western blotting. A 57 kDa T. canis-specific antibody fraction(TcES-57) was identified by western blotting and labelling with anti-Toxocara antibodies(from experimental rabbits and human patients) and tracing with anti-human or anti-rabbit peroxidase conjugate. No protein fraction of 57 kDa was detected in ES or larval antigens collected from T. canis, T. vitulorum, A. lumbricoides and N. americanus. Using TcES-57, a specific anti-serum was produced in rabbits and a double sandwich ELISA was developed. This test was validated using known seropositive sera from toxocariasis patients, sera from A. lumbricoides or N. americanus patients, and 50 serum samples from cats. These tests revealed that TcES-57 antigen is specific to T. canis infection and does not cross react with sera of other related infections. Thus, ELISA based on TcES-57 antigen was proven to be an effective tool in the diagnosis of toxocariasis and studies on the role of T. canis in the epidemiology of human toxocariasis.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.99-103
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• 2000

The green lacewing, Chrysopa pallens (Rambur), was reared on artificial diets supplementedwith natural products such as lyophilized beef liver, silk worm pupae powder and drone honey beepowder. The performance of the green lacewing was improved siginificantly on only the diet to whichadded 1 % of silk worm pupae powder (wt/v). Larval developement was completed in 15.6 f 0.99 days,cocoon weight was 16.4f2.94 mg, and suvival to adult was 89.3% at 27$^{\circ}$C. And another diet made bymixing 4 part of lyophilized beef liver powder, 4 part of lyophilized beef powder, 3 part of lyophilizedchicken egg, 4 part of sucrose, and 50 mg each of vitamin C and Vanderzant's vitamin B mixture waspromising. Larval development of the green lacewing on this diet was completed in 12.7 f 1.45 days at27"C, pupal weight was 18.1 f 2.24mg, suvival to adult was loo%, and 633f70.4 eggs were laid in 20days after beginning of oviposition.ginning of oviposition.

• Molecules and Cells
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• v.40 no.2
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• pp.151-161
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• 2017

Proper synaptic function in neural circuits requires precise pairings between correct pre- and post-synaptic partners. Errors in this process may underlie development of neuropsychiatric disorders, such as autism spectrum disorder (ASD). Development of ASD can be influenced by genetic factors, including copy number variations (CNVs). In this study, we focused on a CNV occurring at the 16p11.2 locus in the human genome and investigated potential defects in synaptic connectivity caused by reduced activities of genes located in this region at Drosophila larval neuromuscular junctions, a well-established model synapse with stereotypic synaptic structures. A mutation of rolled, a Drosophila homolog of human mitogen-activated protein kinase 3 (MAPK3) at the 16p11.2 locus, caused ectopic innervation of axonal branches and their abnormal defasciculation. The specificity of these phenotypes was confirmed by expression of wild-type rolled in the mutant background. Albeit to a lesser extent, we also observed ectopic innervation patterns in mutants defective in Cdk2, Gq, and Gp93, all of which were expected to interact with Rolled MAPK3. A further genetic analysis in double heterozygous combinations revealed a synergistic interaction between rolled and Gp93. In addition, results from RT-qPCR analyses indicated consistently reduced rolled mRNA levels in Cdk2, Gq, and Gp93 mutants. Taken together, these data suggest a central role of MAPK3 in regulating the precise targeting of presynaptic axons to proper postsynaptic targets, a critical step that may be altered significantly in ASD.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.123-128
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• 2017

Hangover due to alcohol consumption causes social and physical problems. There is a growing interest in edible insects worldwide. We have previously published a new technology to make hard mature silkworm, Bombyx mori, into edible form, steamed and freeze-dried mature silkworm larval powder (SMSP). In this study, AIN-76 or SMSP (0.1 and 1 g/kg rat body weight) containing diets in SD rats were pretreated for 2 weeks, and ethanol (3 g/kg rat body weight) was administered as an oral gavage and sacrificed after 3 hours. As a result, blood alcohol and aldehyde levels were significantly decreased in SMSP fed rats. In addition, liver injury markers, alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly decreased in SMSP group compared to ethanol group. $TNF-{\alpha}$, an inflammatory cytokine, and malondialdehyde (MDA), an oxidative stress marker, also showed a dose-dependent decrease in the group receiving SMSP. Conclusively, consumption of SMSP not only reduced hangover induced by ethanol, but also decreased liver damage, oxidative stress, and inflammatory response.

• Journal of Sericultural and Entomological Science
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• v.48 no.2
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• pp.37-40
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• 2006

A new silkworm variety "Daepoongjam" for spring rearing season is $F_1$ hybrid between Jam151, a Japanese race bred from introduction breeding and Jam152, a Chinese race from introduction breeding. In the local adaptatability test performed at 8local areas in spring of 2005, the heavy cocoon yield of "Daepoongjam" was recorded 6% higher than "Kumokjam". The concentration of Deokynojirimycin (DNJ) was measured 4.22 mg in "Daepoongjam", similar to "Kumokjam" (4.26 mg). In the Peacilomyces tenuipes production ability test, the pupal weight of "Daepoongjam" was 1.61 g/individual 13% heavier than "Kumokjam" (1.41 g/individual)

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.1-4
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• 2012

A new silkworm variety "DaeBakJam" for spring rearing season is F1 hybrid between Jam155, a japanese race bred from introduction breeding and Jam156, a chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2010, the hatchability rate of DaeBakJam was recorded 96% similar to DaePoongJam. The larval period was 3 hour shorter than DaePoongJam. The pupation percentage was recorded 0.2% higher than DaePoongJam. Single cocoon weight (2.72 g) and Cocoon yield (25.4 kg) was higher than DaePoongJam. The concentration of DNJ was measured 3.28 mg in DaePoongJam, lower than DaePoongJam (3.48 mg). Test of the artificial diet of DaeBakJam showed bad adaptability of the artificial diet for larval period from 1st to 3rd, but KumOkJam showed excellent adaptability of the artificial diet. In the Paecilomyces tenuipes production ability test, the pupal weight of DaeBakJam was 1.69 g/individual, higher than DaePoongJam (1.59 g/individual).

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.5-9
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• 2012

A new silkworm variety SooOkJam for spring rearing season is F1 hybrid between Jam157, a japanese race bred from introduction breeding and Jam158, a chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2011, the hatchability rate of SooOkJam was recorded 2% higher than the authorized silkworm variety, DaePoongJam. The larval period was recorded 12 hour shorter than DaePoongJam. The pupation percentage was recorded 0.8% higher than DaePoongJam. Single cocoon weight (2.40 g), Cocoon yield (22.6 kg) and best cocoon rate (95.5%) was similar to DaePoongJam. The concentration of DNJ was measured 4.12 mg in SooOkJam, lower than DaePoongJam (4.45 mg). Test of the artificial diet of SooOkJam showed excellent adaptability of the artificial diet for larval period from 1st to 3rd, similar to KumOkJam. In the Paecilomyces tenuipes production ability test, the pupal weight of SooOkJam was 1.61 g/individual, similar to DaePoongJam (1.65 g/individual).