• 미생물학회지
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• 제26권3호
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• pp.188-196
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• 1988

유당함유 울질로부터 알코올을 생산하기 위해 Saccharomyces cerevisiae X 2180-1 A, Candida pseudotropicalis ATCC 8619와 CBS 607 사이에 속간 및 종내 원형질체 융합을 시도하였다. S. cerevisiae X2180-1A(ade rho)와 C. pseudotropicalis CBS 607 (his met)간의 속간 융합율은 $1.0\times 10^{-5}$ C. pseudotropicalis ATCC 8619와 CBS 607 사이의 종내 융합율은 $3.9\times 10^{-6}$ - $1.0\times 10^{-4}$ 이었다. 이는 bovme serum albumin, myoinositol 그리고 ergosterol을 처리하지 않고 얻어진 원형질체 융합율과 비교해서 약 2-3.5배의 증가를 보여 이들 화합물이 속간 및 종내 융합을 증진시킨 것으로 사료된다. DNA 함량, 핵염색, UV 조사에 따른 생존력 비교 그리고 형질분리 후 교잡형 출현 등을 통해서 속간 (S. cerevisiae와 C. pseudotropicalis)과 종내 ( C psemdotropica/is strains) 융합체에서 핵융합이 일어난 것을 알 수 있었다. 또한 양친형과 비교해서 알코올 생성능이 증가된 종내 융합체를 얻었다.

• Journal of Microbiology
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• 제45권6호
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• pp.505-509
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• 2007

An obligately aerobic bacterium, strain KOPRI $20902^T$, was isolated from a marine sediment in Ny-${\AA}$lesund, Spitsbergen Islands, Norway. Cells were irregular rods and motile with polar monotrichous flagellum. The optimum growth temperature was $17-22^{\circ}C$. Cells grew best in pH 7.0-10.0 and 3-4% sea salts (corresponding to 2.3-3.1% NaCl). The novel strain required $Ca^{2+}$ or $Mg^{2+}$ in addition to NaCl for growth. Sequence analysis of 16S rRNA gene revealed that the Arctic isolate is distantly related with established species (<92.4% sequence similarity) and formed a monophyletic group with Cellvibrio, which formed a distinct phylogenetic lineage in the order Pseudomonadales. Predominant cellular fatty acids [$C_{16:1}\;{\omega}7c/15:0$ iso 2OH (45.3%), $C_{16:0}$ (18.4%), ECL 11.799 (11.2%), $C_{10:0}$ 3OH (10.4%)]; DNA G+C content (37.0 mol%); nitrate reduction to nitrogen; absence of aesculin hydrolysis, N-acetyl-${\beta}$-glucosaminidase and esterase; no assimilation of arabinose, galactose, glucose, lactose, maltose, and trehalose differentiated the strain from the genus Cellvibrio. Based on the phylogenetic and phenotypic characteristics, Dasania marina gen. nov., sp. nov. is proposed in the order Pseudomonadales. Strain KOPRI $20902^T$ (=KCTC $12566^T$=JCM $13441^T$) is the type strain of Dasania marina.