• Title/Summary/Keyword: lactic acid starter

Search Result 291, Processing Time 0.03 seconds

Antihypertensive peptides from whey proteins fermented by lactic acid bacteria

  • Daliri, Eric Banan-Mwine;Lee, Byong H.;Park, Byun-Jae;Kim, Se-Hun;Oh, Deog-Hwan
    • Food Science and Biotechnology
    • /
    • v.27 no.6
    • /
    • pp.1781-1789
    • /
    • 2018
  • In this study, whey proteins were fermented with 34 lactic acid bacteria for 48 h at $37^{\circ}C$ and their ability to inhibit angiotensin 1-converting enzyme (ACE) activity were compared. All the lactic acid bacteria displayed varying proteolytic abilities in whey. Their fermentates also displayed varying abilities to inhibit ACE in vitro. Seven fermentates showed strong ACE inhibitory abilities between $84.70{\pm}0.67$ and $52.40{\pm}2.1%$ with $IC_{50}$ values between $19.78{\pm}1.73$ and $2.13{\pm}0.7mg/ml$. Pediococcus acidilactici SDL1414 showed the strongest ACE inhibitory activity of $84.7{\pm}0.67%$ ($IC_{50}=19.78{\pm}1.73{\mu}g/ml$). Mass spectrometry revealed that more than half (57.7%) of the low molecular weight peptides (< 7 kDa) in the P. acidilactici SDL1414 fermented samples were ACE inhibitory peptides. Our results show that P. acidilactici SDL1414 could be used as a starter culture in the dairy industry to develop antihypertensive functional foods for hypertension management.

Isolation and Characterization of Plant-Derived Lactic Acid Bacteria as Potential Probiotic (잠재적 생균제로서 식물 젖산균의 분리 및 특성)

  • Kim, Jeong-Do;Park, Sung-Bo;Lee, Na-Ri;Jeong, Jin-Ha;Lee, Hee-Seob;Hwang, Dae-Youn;Lee, Jong-Sup;Jeong, Seong-Yun;Son, Hong-Joo
    • Microbiology and Biotechnology Letters
    • /
    • v.39 no.3
    • /
    • pp.308-312
    • /
    • 2011
  • Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.

Changes in Carotene Content of Chinese Cabbage Kimchi Containing Various Submaterials and Lactic Acid Bacteria during Fermentation (배추김치의 숙성중 부재료와 젖산균에 따른 Carotene 의 함량변화)

  • 장경숙;김미정;오영애;강명수;김순동
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.20 no.1
    • /
    • pp.5-12
    • /
    • 1991
  • the Chinese cabbage kimchi was fermented with the various submaterials such as hot pep-per garlic ginger leek green onion fermented anchovy juice and sugar according to the average contents of each submaterial described in the 39 kinds of references. And then the effects of each submaterial and lactic acid bacteria such as L. brevis. Leu. mesenteroides. P cerevisiae and L. plantarum on the content of carotenes were investigated, The major carotene in kimchi was $\beta$-carotene. And also $\delta$-carotene and $\alpha$-carotene were detected. Contents of $\beta$-carotene and total carotene were high in the kimchi containing leek red pepper powder green onion and fermented anchovy juice as a submaterial. But the kimchi containing or omitting the other submaterials were litter affected to the contents of carotene. Contents of $\beta$-carotene and total carotene were high in kimchi fermented with Leu. msenteroides L. brevis and P. cerevi-siae as a starter but was low with L plasntarum.

  • PDF

Studies on the Conditions of Cultivation and Drying of Lactic Acid Bacteria part I. Optimum Cultural Conditions of Lactic Acid Bacteria (유산균의 배양 및 건조조건에 관한 연구 제1보 유산균의 배양조건에 관한 연구)

  • Yoon, Joo-Chun;Kim, Kap-Soo;Shin, Won-Cheol;Lee, Keun-Eok;Choi, Seung-Rack;Yu, Ju-Hyun
    • Journal of Industrial Technology
    • /
    • v.1
    • /
    • pp.61-66
    • /
    • 1981
  • In order to use as a starter after drying, optimum growth conditions of Str. thermophilus and L. bulgaricus were investigated. The results were as follows. The growth of Str. thermophilus was reached at stationary phase after 12 hours incubation and L. bulgaricus was after 6 hours. Str. thermophilus was grown well, in the medium containing 10% of skim milk, 2% of sucrose, 0.1% of KCl and 0.015% (v./v.) of soy-sauce. In the case of L. bulgaricus, 10% of skim milk, 2% of glucose, 0.5% of bacto-peptone, 0.1% of $K_2HPO_4$ and 0.02% of pyridoxine was suitable for the maximum growth.

  • PDF

Probiotic Property and Anti-Obesity Effect of Lactiplantibacillus plantarum KC3

  • Kim, Seulki;Huang, Eunchong;Ji, Yosep;Holzapfel, Wilhelm Helnrich;Lim, Sang-Dong
    • Food Science of Animal Resources
    • /
    • v.42 no.6
    • /
    • pp.996-1008
    • /
    • 2022
  • Lactic acid bacteria are representative probiotics that have beneficial effects on humans. Nineteen strains among the 167 single strains from kimchi was selected and their physiological features were investigated. The selection of a strain was based on strong enzyme (lipase, α-amylase, and α-glucosidase) inhibitory activities and anti-obesity effects in the adipocytes. For the final selection, the strain Lactiplantibacillus plantarum KC3 was tested for its potential as a starter. To assess its functionality, a freeze-dried culture of L. plantarum KC3 was administered to a diet-induced obese mouse model receiving a high-fat diet. The animal group administered with L. plantarum KC3 showed significant body weight loss during the 12-week feeding period compared to the high-fat control group. This study investigated the physiological characteristics of selected strain and evaluated its potential as an anti-obesity probiotic in mice.

Production of Conjugated Linoleic Acid by Lactobacillus acidophilus and Their Industrial Application (Lactobacillus acidophilus의 산업적 이용과 CLA 생성)

  • Baick, Seung-Cheon;Chung, Gwan-Seop;Kim, Cheol-Hyeon
    • Journal of Dairy Science and Biotechnology
    • /
    • v.22 no.2
    • /
    • pp.119-130
    • /
    • 2004
  • Application of lactic acid bacteria in the markets are divided into four categories: dairy industry, health food industry, animal feed industry and pharmaceutical industries. Recently, Lactobacillus acidophilus have been used in the food industry and have obtained great attention as key cultures for health benefit. Since commercial application of L. acidophilus has become a common practice, characterization of these cultures were made. Futhermore, the strains selected should produce a final dairy product possessing good taste and acceptable body and texture, a selection step that cannot be achieved unless the product is actually manufactured. Conjugated linoleic acid (CLA) have been recognized as antioxidants, cancer inhibitors, cholesterol depressing agents, and growth promoting factors. Food products from ruminants, particularly dairy products, are the major dietary source of CLA f3r humans. The CLA content in yogurt or cheese can be increased by action of the starter cultures. The finding of the production of CLA by food starter culture opens interesting perspectives far the future in producing fermented products enriched in CLA.

  • PDF

Effect of Prepared by Enterococcus sp. and Lactobacillus sp. on the Quality of Barley Bread - I. Identification of Bacterial Strain from Barley Powder and Rheological Properties of Sourdough - (Enterococcus sp.와 Lactobacillus sp. 첨가 sourdough로 제조된 보리식빵의 품질특성 - I. 보리가루에서 분리한 균주의 동정 및 반죽의 물성적 특성 -)

  • Hong, Jeong-Hoon;Kim, Kyoung-Ja
    • Journal of the Korean Society of Food Culture
    • /
    • v.16 no.4
    • /
    • pp.354-360
    • /
    • 2001
  • A Lactic acid bacterium for barley bread was isolated from barley powder and was identified as Enterococcus sp. It was used as a starter for barley bread and quality of bread was compared with the bread prepared by conventional starter 1. The pH of bread making process using Lactobacillus sanfrancisco starter was the lowest among tested starters. while the titratable acidity(TTA) of the strain was the highest, followed by Enterococcus sp.. 2. In valorimeter value(v/v), control was 70, but dough using all starter was 60. The peak time and stability of dough using Lactobacillus sanfrancisco were the highest among tested starters. 3. Extensibility of dough using Enterococcus sp. was the highest among tested starters, followed by Lactobacillus sanfrancisco. Proper extensibility and maximum resistance were observed in Lactobacillus sanfrancisco.

  • PDF

Fermentation Characteristics of Starter Cultures in Lactose-Hydrolyzed Milk for the Elderly (유당분해 우유를 이용한 고령자용 요구르트 배양)

  • Oh, Sejong;Kim, Bum Keun;Chun, Yong-Gi;Park, Dong June
    • Journal of Dairy Science and Biotechnology
    • /
    • v.39 no.1
    • /
    • pp.20-26
    • /
    • 2021
  • Lactase (β-galactosidase) is abundant in the small intestine during early childhood and gradually decreases with age. Lactic acid bacteria (LAB) present in yogurt could survive in the stomach, and lactase produced by these LAB can aid in lactose breakdown in the small intestine, thereby reducing lactose intolerance. This study aims to provide preliminary data for development of lactose-free yogurts for the elderly, and investigate the effect of lactose-hydrolyzed milk on the growth of starter cultures. The pH during yogurt fermentation using lactose-free milk was slightly higher at 2 and 4 h of incubation, but reached 4.5 at the end of incubation, similar to that of the yogurt prepared from regular milk. The number of viable cells of Streptococcus thermophilus reached 108 CFU/mL after 2 h of incubation and increased to 109 CFU/mL after 4 h of incubation. During yogurt fermentation, the viable cells of Lactobacillus species and Bifidobacterium longum did not affect lactose hydrolysis. Although lactose-hydrolyzed milk did not promote the growth of starter cultures, manufacturing yogurt with lactose-free milk could be beneficial for the intestinal health of lactose-sensitive elderly.

Preparation of various soy cheese and their physicochemical and sensory characteristics (대두 Cheese의 제조와 그의 일반 성분 및 수응도에 관한 연구)

  • Yang, Kyung-Soon;Yoon, Sun
    • Korean journal of food and cookery science
    • /
    • v.3 no.2
    • /
    • pp.75-80
    • /
    • 1987
  • For the manufacture of soybean cheese, curds were made from soy milk using either lactic starter or CaCl$_2$and ripened for 3 weeks. When papain was added during ripening, the amout of soluble protein increased. The yields of 3 soybean cheese were 76~88%, indicating that considerable loss of soy protein did not occur during the processing. During the period of ripening the change of moisture content was about 1 n. Total acidity of 3 soy cheese increased, demonstrating the production of lactic acid through lactic fermentation. Texture determined by Instron showed that 3 soy cheese had their own characteristic texture profile curve and that the cheese without papain treatment received the highest textural parameters. 3 manufactured soy cheese received 3-4 (good-very good) sensory scores in appearance, flavor and texture. General acceptibility of 3 cheese were evaluated as desirable or very desirable.

  • PDF

Development of the Formula for Natural Bread-making Starter (천연제빵 발효 Starter의 개발)

  • 이종열;이시경;조남지;박원종
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.32 no.8
    • /
    • pp.1245-1252
    • /
    • 2003
  • A starter formulation was developed to substitute a baker's yeast with natural starter when manufacturing bread products. To develop an active starlet, starter was formulated varying with types of wheat flours, level of water contents and various nutrients. Activities of starter were investigated in terms of viable counts of microbes and change of pH and total titratible acidity Domestic wheat flours contain 100 times more number of lactic acid bacteria than yeast regardless of types of wheat flours. The more protein contents in wheat flours, the more stable microbes in starter. This was considered to be the result of buffering effect of wheat proteins. The optimum level of protein content to ensure the activity of starter was more than 12.0%. Optimum level of water content in active starter was 110% based on strong flour. The more water or the less water had the tendency of decreasing viable counts of microbes. Addition of salt and sucrose had increased the activity of starters. However oligosaccharides did not affect the activity of starter. The optimum concentrations of salt and sucrose were 1.0% and 5.0% respectively. Bread with the starter was higher scored than breads with yeast in terms of all the quality and sensory characteristics except their volumes. In conclusion, a starter formulated with strong flour 100%, water 110%, salt 1% and sucrose 5% was considered to have high potential as a substitute of yeast in making natural bread.