• 생명과학회지
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• 제16권4호
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• pp.632-639
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• 2006

본 연구에서는 lacZ transcriptional fusion plasmid를 이용하여 광합성 세균인 Rhodobacter sphaeroides에서의 7가지 광합성유전자 (puf, puc, puhA, bchC, bchE, bchF, bchI) 발현의 경향과 조절을 조사하였다. R. sphaeroides에서 puhA와 bchI를 제외한 모든 광합성유전자들이 호기적 조건과 비교했을 때 혐기적 조건에서 더욱 강하게 발현되었다. puhA 유전자는 bchFNBHLM-RSP0290과 operon을 형성하며, bchI 유전자는 crtA와 operon을 이루는 것으로 나타났다. 광합성 조건에서 자란 R. sphaeroides의 puf, puc, bchCXYZ operon의 발현은 빛의 세기에 비례하는 반면, bchFNBHLM(RSP0290 puhA) operon의 발현은 빛의 세기에 반비례 하였다. bchEJG의 발현은 $10\;W/m^2$의 빛이 조사된 광합성 조건에서 제일 낮았으며, $100\;W/m^2$의 빛의 광합성 조건에서 가장 높았다. R. sphaeroides의 산소인지와 빛 인지에 관련된 세 가지 주요 조절기작에 의한 광합성유전자 조절은 다음과 같다. puf와 bchC는 PpsR repressor와 PrrBA two-component system에 의해 조절된다. 그리고 puc operon은 PpsR, FnrL, PrrBA system에 의해 조절된다. bchE의 발현은 FnrL과 PrrBA system에 의해 조절되는 반면, bchF는 오로지 PpsR에 의해서만 조절된다. PpsR repressor는 강한 세기의 빛 조건에서 bchf 발현억제의 원인이 되며, FnrL은 그 자체가 산소를 인지하는 기능 이외에도 세포질의 산화/환원 상태의 인지에 관련될 것으로 보인다.

• 한국지구과학회지
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• 제45권2호
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• pp.136-146
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• 2024

본 논문은 서해 군산분지 퇴적층 내에서 발견되는 다양한 화성암들을 탄성파 반사상에 따라 분류하고 그 공간적 분포 양상을 도시하는 과정에 대해 상술한다. 연구지역인 서해 대륙붕 군산분지의 북동소분지, 남서소분지, 남동소분지 내에서 총 6종류의 분출 화성암 반사상과 3종류의 관입 화성암 반사상이 중생대 후기 백악기, 신생대 에오세 및 전기 마이오세, 신생대 제4기 층에서 발견되었다. 분출 화성암은 그 크기, 외형, 내부 층리구조에 따라 (1) 높이 200 m 이하의 고깔형 화산체들로 1회성 단성화산 분화를 통해 형성된 것으로 추정되는 단성화산체(E.mono), (2) 단성화산체들이 모여 높이 500 m 이내의 하나의 화산체를 형성하는 단성화산복합체(E.comp), (3) 높이 1 km 이상 직경 15 km 이상으로 내부에 복수의 층이 인지되는 성층화산체(E.strato), (4) 기반암 저지대의 정단층 주변에서 고반사 특성을 보이는 열극 분출암(E.fissure), (5) 저각 사면부와 내부에 직경 약 2 km 화도 구조를 보여주는 마르-다이아트림(E.maar), (6) 퇴적층을 교란하는 직경 1 km 이내 파이프형 구조를 갖는 열수분출공(E.vent)으로 구성된다. 관입 화성암은 상부 퇴적층의 변형구조를 통해 인지되며, (1) 수평형, 계단형, 종지형 등 다양한 수직/수평 관입 기하를 나타내는 암맥 및 암상(I.dike/sill), (2) 직경 1-3 km의 뿔 또는 기둥 모양 관입암체인 화성암주(I.stock), (3) 상부 퇴적층을 광범위하게 변형시키는 폭 10 km 이상의 관입암체인 저반/병반(I.batho/lac)으로 구성된다. 분출 화성암은 주로 군산분지 남서소분지 내 백악기 및 신생대 고제3기 에오세 층 내에서 인지되며, 관입암체는 주로 남동 및 남서 소분지 내에서 발견된다. 열수분출공은 군산분지 전역에서 발견되며 활동시기는 전반적으로 중기 마이오세 이후로 일부는 현재까지 활동중인 것으로 보인다.

• Journal of Microbiology and Biotechnology
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• 제3권1호
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• pp.6-11
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• 1993

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 춘계학술대회
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• pp.80-80
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• 1997

The object of this study was to develop a gene therapy strategy for ovarian cancer. We have previously shown that AV with a L-plastin (LP) promoter infects breast and ovarian cancer cells and expressed ${\beta}$-galactosidase cDNA in preference to normal fibroblast cells and hematopoietic cells. We now report on the cytotoxicity of Ad.LP.CD, an AV carrying a CD cDNA which converts the pro-drug, 5-Fluorocytosine (5-FC) into the toxic drug 5-Fluorouracil (5-FU). Infection of Ad.LP.CD into either 293 cells or ovarian cancer cells generated the functional CD as measured by HPLC analysis. Using a ratio of AV to OVCAR3 cell of 100 and a 5-FC concentration of 100 ${\mu}$M, we achieve an over 95 % of cell growth inhibition. We are using flow cytometry analysis for ${\beta}$ -galactosidase and ovarian cancer associated folate receptor to screen primary ascites samples for infectivity after infection with an adenoviral vector, i.e., Ad.LP.LacZ. This vector system may be of value in the treatment of microscopic disease of ovarian cancer in the peritoneal cavity.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.1022-1030
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• 2004

The gene encoding Aquifex pyrophilus (Apy) DNA polymerase was cloned and sequenced. The Apy DNA polymerase gene consists of 1,725 bp coding for a protein with 574 amino acid residues. The deduced amino acid sequence of Apy DNA. polymerase showed a high sequence homology to Escherichia coli DNA polymerase I-like DNA polymerases. It was deduced by amino acid sequence alignment that Apy DNA polymerase, like the Klenow fragment, has only the two domains, the $3'{\rightarrow}5'$ exonuclease domain and the $5'{\rightarrow}3'$ polymerase domain, containing the characteristic motifs. The Apy DNA polymerase gene was expressed under the control of T7lac promoter on the expression vector pET-22b(+) in E. coli. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $UNO^{TM}$ Q column chromatographies. The optimum pH of the purified enzyme was 7.5, and the optimal concentrations of KCl and $Mg^{2+}$ were 20 mM and 3 mM, respectively. Apy DNA polymerase contained a double strand-dependent $3'{\rightarrow}5'$ proofreading exonuclease activity, but lacked any detectable $5'{\rightarrow}3'$ exonuclease activity, which is consistent with its amino acid sequence. The somewhat lower thermostability of Apy DNA polymerase than the growth temperature of A. pyrophilus was analyzed by the comparison of amino acid composition and pressure effect.

• 미생물학회지
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• 제30권6호
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• pp.539-545
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• 1992

Lactobacillus casei SM-M1 의 플라스미드로부터 phospho-$\beta$-galactosidase gene 을 갖는 DNA 를 E. coli 에 클로닝한 pPLac15(13kb) 의 재조합 플라스미드를 제조하였다.(15). pPLac15 DNA 를 분리하여 제한효소로 처리하여 제한효소 지도를 작성하였다. Phospho-$\beta$-galactosidase 유전자의 발현을 높이기 위하여 lac promoter 를 가진 pUC18 의 PstI 위치에 클닝하여 pPLac18 을 제조하였으며, 이것을 다시 EcoRI 으로 절단하여 pUC 18 에 클로닝하여 얻은 pPLac23 (7.6 kb) 를 얻었다. Phospho-$\beta$-galactosidase 효소활성은 pPLac23 의 형질전환주인 E. coli SW-23 에서는 pPLac15 를 가진 형질전환주인 E. coli SW-15 보다 약 1.8 배의 효소의 활성을 나타내었으며 pPLac18 을 가진 E. coli SW-18 보다는 약간 높은 활성을 나타내었다.

• 천문학논총
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• 제15권spc1호
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• pp.93-102
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• 2000

We have reviewed the magnetic activity in close binaries. Solar like magnetic activity indicators such as photometric spots, chromo spheric emission, coronal X-ray and radio emission, and flare activity are commonplace in many cool stars with convective envelopes. Using the UV spectra we confirmed the strength of stellar activity increases with more rapid rotation and later spectral types which corresponds to the increasing depth of the star's convective envelope. Apart from very young stellar objects such as T Tauri stars, the stars with the highest levels of activity are close binary systems composed of cool stars, i.e., the chromospherically active binaries such as RS CVn, BY Dra, W UMa and related systems. The IUE low and high dispersion spectra of V711 Tau, VW Cep and SW Lac are used for ultraviolet photometry and for a variation study of chromospheric activity. Evidence of chromospherically activity is indicated by the intensity variation of the Mg II emission line with orbital phase.

• The Journal of Korean Physical Therapy
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• 제10권2호
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• pp.113-125
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• 1998

The neurotropic psudorabies virus(PRV) to replicate within neurons is very useful pathogen for neuronal tracing. I carried out this study to investigate the parametric analysis on the viral infection in the rat circadian control center with two genetically engineered strains out of PRV. The two strains are isogenic with the attenuated Bartha strain of PRV ; in one strain a lacZ reporter gene was inserted into the gC locus (PRV-BaBlu ; $4.75\times10^8pfu/ml$) and the other strain contained a PRV envelope glycoprotein gene(PRV-D ; $2.5\times10^8pfu/ml$) theat is absent in PRV-BaBlu. simultaneous or temporally separated sequential injection of$2{\mu}l$ of each strain into the vetreous body of eye produced a course of transsynaptic infection of retinohypothalamic circuitry. The results were as follows; 1. PRV-BaBlu and PRV-D infected the suprachiasmatic nucleus in hypothalamus and intergeniculate leaflet in lateral geniculate nucleus of thalamus. 2. The rate of PRV infection was dependent upon PRV strain. 3. Pre-infected neurons by PRV-D were interfered with the replication of PRV-BaBlu. 4. Dual injection of PRV-D and PRV-BaBlu showed more virulent than the parental strain.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.139-146
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• 2000

7- Bromomethylbenz[a]anthracene is a known mutagen and carcinogen. The two major DNA adducts produced by this carcinogen, i.e., $N^2$-(benz[a]anthracen-7-yl methyl)-2'-deoxyguanosine (2, b[a]$a^2$G) and $N^6$-(benz[a]anthracen-7-ylmethyl)-2'-deoxyadenosine (4, b[a]$a^6$/A), as wel 1 as the simpler benzylated analogs,$N^2$-benzyl-2'deoxyguanosine (1, $bn^2$G) and $N^6$-benzyl-2'-deoxyadenosine (3, $bn^6$/A), were prepared by direct aralkylation of 2'-deoxyguanosine and 2'-deoxyadenosine. To determine the site-specific mutagenicity of these bulky exocyclic amino-substituted adducts, the suitably protected nucleosides were incorporated into 16-base oligodeoxyribonucleotides in place of a normal guanine or adenine residues which respectively are part of the ATG initiation codon for the lac Z' \alpha-complementation gene by using an in situ activation approach and automated phosphite triester synthetic methods. The base composition and the incorporation of the bulky adducts into synthetic oligonucleotides were characterized after purification of the modified oligonucleotides by enzymatic digestion and HPLC analysis.

• 천문학논총
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• 제30권2호
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• pp.453-455
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• 2015

In this paper we introduce the Plasma Physics of Active Galactic Nuclei project, which is an ongoing experiment with Korean VLBI Network (KVN) and KVN and VERA Array (KaVA) to study multi-frequency polarimetric properties on parsec scales of active galaxies. The goal of the project is to improve our understanding of fundamental jet physics, especially evolution of the relativistic outflow coupled with the large-scale magnetic field. We selected six radio-loud AGN as our targets. So far we (i) detected resolved emissions regions at 86 and 129 GHz on VLBI scales, (ii) constructed 2D spectral index maps of the outflows, and (iii) found polarizations at 22 and 43 GHz for a few targets. Here we present spectral index distributions of 3C 120 between 22 and 43 GHz and a linear polarization map of BL Lac at 43 GHz obtained with KVN.