• Journal of Veterinary Clinics
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• v.41 no.2
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• pp.88-94
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• 2024

Pet rabbits are affected by the highly contagious ectoparasite Psoroptes (P.) ovis, which carries significant economic implications for the global rabbit industry. Accurate identification of the mite species remains essential to implement effective treatment and control strategies. Two approximately one-year-old female pet rabbits were admitted to the Veterinary Teaching Hospital of Chungbuk National University due to excessive scratching of the ears and the presence of waxy debris within the ear canals. Mites were isolated from the waxy debris extracted from the ear canals and subsequently identified as Psoroptes spp. through microscopic examination. Species confirmation was achieved through mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis. The analysis revealed the mites to be P. ovis based on cox1 gene sequences. The deposited GenBank accession numbers for these sequences are OR985022 and OR985023. This represents the first report of mitochondrial cox1 gene sequences of P. ovis isolated from pet rabbits in South Korea.

• Parasites, Hosts and Diseases
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• v.51 no.1
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• pp.133-137
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• 2013

This study aimed to measure the levels of interferon-gamma (IFN-${\gamma}$), tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate ($NO_x$) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-${\gamma}$, TNF-${\alpha}$, IL-1, IL-6, and $NO_x$. Cytokines were assessed by ELISA quantitative sandwich technique, and $NO_x$ was measured by the modified Griess method. Cytokine levels (IFN-${\gamma}$, TNF-${\alpha}$, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of $NO_x$ were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

• Journal of Veterinary Science
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• v.23 no.4
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• pp.57.1-57.9
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• 2022

Background: Classical swine fever virus (CSFV), the causative agent of classical swine fever (CFS), is a highly contagious disease that poses a serious threat to Chinese pig populations. Objectives: Many provinces of China, such as Shandong, Henan, Hebei, Heilongjiang, and Liaoning provinces, have reported epidemics of CSFV, while the references to the epidemic of CSFV in Yunnan province are rare. This study examined the epidemic characteristics of the CSFV in Yunnan province. Methods: In this study, 326 tissue samples were collected from different regions in Yunnan province from 2015 to 2021. A reverse transcription-polymerase chain reaction (RT-PCR), sequences analysis, and phylogenetic analysis were performed for the pathogenic detection and analysis of these 326 clinical specimens. Results: Approximately 3.37% (11/326) of specimens tested positive for the CSFV by RT-PCR, which is lower than that of other regions of China. Sequence analysis of the partial E2 sequences of eleven CSFV strains showed that they shared 89.0-100.0% nucleotide (nt) and 95.0-100.0% amino acid (aa) homology, respectively. Phylogenetic analysis showed that these novel isolates belonged to the subgenotypes 2.1c and 2.1d, with subgenotype 2.1c being predominant. Conclusions: The CSFV was sporadic in China's Yunnan province from 2015 to 2021. Both 2.1c and 2.1d subgenotypes were found in this region, but 2.1c was dominant.

• Parasites, Hosts and Diseases
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• v.51 no.2
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• pp.197-201
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• 2013

A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.

• Steel and Composite Structures
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• v.16 no.2
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• pp.117-133
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• 2014

Natural vibration of truss cable structures is analyzed based upon the general structural analysis software ANSYS, energy variational method and Rayleigh method, the calculated results of three methods are compared, from which the characteristics of free-vibration are obtained. Moreover, vertical seismic response analysis of truss cable structures is carried out via time-history method. Introducing three natural earthquake waves calculated the results including time-history curve of vertical maximal displacement, time-history curve of maximal internal force. Variation curve of maximal displacement of node along span, and variation curve of maximal internal force of member along span are presented. The results show the formulas of frequencies for truss cable structures obtained by energy variational method are of high accuracy. Furthermore, the maximal displacement and the maximal internal force occur near the 1/5 span point. These provide convenient and simple design method for practical engineering.

• Geomechanics and Engineering
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• v.21 no.5
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• pp.423-432
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• 2020

Hydraulic conductivity is one of the engineering properties of soil. This study focusses on the influence of cement content on the hydraulic conductivity of cemented sand, which is investigated based on the results from numerical analysis and laboratory testing. For numerical analysis the cemented samples were scanned using X-ray Computed Tomography (CT) while laboratory testing was carried out using a triaxial setup. Numerical analysis enables us to simulate flow through the sample and provides insight to the microstructure. It quantifies the pore volume, proportion of interconnected voids and pore size distribution in both cemented and uncemented samples, which could be computed only through empirical equations in case of laboratory testing. With reduction in global voids, the interconnecting voids within the samples also reduce with cement content. Gamma cumulative distribution function is used to predict the percentage of voids lesser than a given pore volume. Finally, the results obtained from both numerical analysis and laboratory testing are compared.

• Journal of Technologic Dentistry
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• v.36 no.3
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• pp.159-164
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• 2014

Purpose: The purpose of this study was analysis of quality of fixed prostheses fabricated by dental CAD/CAM system. Methods: The ten same cases of stone models were manufactured by dental scannable model, and stone models were scanned using the dental scanner for changing digital model. Ten digital models were completed. The design of zirconia core for zirconia based all-ceramic crown was conducted by the dental CAD program. The samples were fabricated using the pre-sintered zirconia block by dental milling machine. Marginal gaps were analyzed using by silicone replica technique at the eight parts for quality analysis of samples. One-way ANOVA was used for statistical analysis(${\alpha}=0.05$). Results: The mean for marginal gaps were $93.2{\sim}99.1{\mu}m$ at the eight parts. One-was ANOVA didn't show significant differences(p=0.089). Conclusion: The fixed dental prostheses fabricated by dental CAD/CAM were within clinically acceptable range.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.1-7
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• 1980

Less-volatile organic components in leaves from aromatic tobaccos of different varieties, both Oriental and Korean types were isolated and concentrated using a simple apparatus with fewer manipulations. Each less-volatile concentrate was then subjected to spectrophotometric recording in the visible range, to thin-layer chromatographic group separation, and high-performance liquid chromatographic profile analysis. The methods allow detection of significant quantitative differences in visible absorption spectra, TLC patterns, and high resolution HPLC profiles among varieties.

• Physical Therapy Korea
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• v.26 no.4
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• pp.20-28
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• 2019

Background: The wall squat exercise has been recommended for strengthening of the lower extremity muscles with maintaining lumbar lordosis. Although squat has been studied to be related to lower extremity extensor strength, the relationship between wall squat and lower extremity extensor strength unclear. Because squat and wall squat are biomechanically different, study on the relationship is needed. Objects: The purpose of this study was to determine the lower extremity extensor strength associated with wall squat performance. Methods: 74 healthy volunteers were recruited to participate in this study. The volunteers were measured hip and knee extensors strength and then performed wall squat exercise for maximum count. Results: We found significant relationships between wall squat performance and hip extensor strength normalized by body weight, knee extensor strength normalized by body weight and the composite value. In a regression analysis, hip extensor strength normalized by body weight explained 29% of the variation in wall squat performance in males and 35% in females. Conclusion: These results demonstrate that hip extensor strength normalized by body weight is critical to wall squat performance in both sexes.

• Analytical Science and Technology
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• v.18 no.2
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• pp.168-172
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• 2005

A comparison study of XRF and $^{31}P$-NMR method for the analysis of phosphorous in lubricating oil has been described. Pure oil and water were used for the observation of matrices effect variation by different analytical methods. Quantitative analysis was carried out by XRF and $^{31}P$-NMR using real samples. Significant difference is observed from the slopes on the calibration curves by the XRF due to the matrices (water: 124.0, oil: 276.6). While the result obtained from XRF showed a large matrix effect, the slopes obtained from $^{31}P$-NMR results of two different matrices, however, are in good agreement ranged of ${\pm}9%$ (water: 4.9, oil: 5.3).