• Korean Journal of Clinical Laboratory Science
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• v.47 no.1
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• pp.39-45
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• 2015

We investigated whether multiple infections can be used as predictors of progression to carcinogenesis in accordance with the cytological diagnosis in women receiving abnormal cytologic diagnosis as analysis genotype and compared to single infection. HPV prevalence is highest in the age of under 30 years old woman, HPV prevalence is started to lower after 30 years old and started to increase over 60 years old as like a U-shape. The specific HPV genotypes is an important factor because increased single infection and reduced multiple infections and appeared single infection with AC in progressing carcinogenesis. HPV 16 revealed the statistical significance at the single infection in squamous cell lesions, and HPV 18 revealed the statistical significance at the single infection in adenocarcinoma with showed HPV 16, 58, 18, 52-type distribution.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.91-97
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• 1999

In this work, a 1.9-kb region of enterococcal plasmid p703/5 was isolated and the nucleotide sequence analysis of the region was performed. One major open reading frame (ORF) was identified encoding a polypeptide of 28 kDa. Database comparisons suggested that the protein showed some homology with other bacterial RepA proteins. Upstream of the ORF, a potential dnaA box, AT-rich region and 22-bp tandemly repeated sequences (DNA iterons), a feature typical for many replication ori sites, were recognized. Deletion analysis using Exonuclease III and several restriction enzymes indicated that the three elements and the gene product from the ORF were essential for replication and that the minimum unit of DNA required for replication resided on the 1.2-kb AvaII subfragment. Thus, this gene product was referred to as RepA.

• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.832-848
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• 2006

Systems biology has recently become an important research paradigm that is anticipated to decipher the metabolic, regulatory, and signaling networks of complex living organisms on the whole organism level. Thus, various research outputs are being generated, along with the development of many tools and resources for systems biology research. Accordingly, this review provides a comprehensive summary of the current resources and tools for systems biology research that will hopefully be helpful to researchers involved in this field. The resources are categorized into the following five groups: genome information and analysis, transcriptome and proteome databases, metabolic profiling and metabolic control analysis, metabolic and regulatory information, and software for computational systems biology. A summary table and some future perspectives are also provided.

• The Plant Pathology Journal
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• v.28 no.4
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• pp.428-432
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• 2012

We determined the complete genome sequence of a Vietnamese isolate of Southern rice black-streaked dwarf virus (SRBSDV). Whole genome comparisons and phylogenetic analysis showed that the genome of the Vietnamese isolate shared high nucleotide sequence identities of over 97.5% with those of the reported Chinese isolates, confirming a common origin of them. Moreover, the greatest divergence between different SRBSDV isolates was found in the segments S1, S3, S4 and S6, which differs from the sequence alignment results between SRBSDV and Rice black streaked dwarf virus (RBSDV), implying that SRBSDV evolved in a unique way independent of RBSDV. This is the first report of a complete nucleotide sequence of SRBSDV from Vietnam and our data provides new clues for further understanding of molecular variation and epidemiology of SRBSDV in Southeast Asia.

• Journal of Microbiology
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• v.41 no.2
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• pp.152-156
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• 2003

Through enrichment on two nutrient agars, 57 Thermus isolates were recovered from 15 hot spring samples taken from the Rehai geothermal area, Tengchong, China. Unique growth characteristics were observed when the strains were transferred from YIM14 medium to Thermus medium. Phylogenetic analysis showed that the 16S rDNA sequences of the isolates and clones from the Rehai geothermal area farmed a monophyletic group on the phylogenetic tree. A secondary structure comparison showed that their 16S rRNAs have unique secondary structure characteristics.

• Genes and Genomics
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• v.40 no.11
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• pp.1137-1148
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• 2018

Freshwater gobies Rhinogobius cliffordpopei and R. giurinus are invasive species with particular concern because they have become dominant and were fierce competitors in the invaded areas in Yunnan-Guizhou Plateau (southwest of China). Information about genetic characteristics of R. giurinus have been published, but there were still no relevant reports about R. cliffordpopei. In present study, the complete mitochondrial genome of R. cliffordpopei was determined, which was 16,511 bp in length with A+T content of 51.1%, consisting of 13 protein-coding genes, 22 tRNAs, 2 ribosomal RNAs, and a control region. The gene composition and the structural arrangement of the R. cliffordpopei complete mtDNA were identical to most of other teleosts. Phylogenetic analyses placed R. cliffordpopei in a well-supported monophyletic cluster with other Rhinogobius fish. But the phylogenetic relationship between genus Rhinogobius and Tridentiger remained to be resolved.

• Journal of the Korean Society of Safety
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• v.34 no.3
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• pp.42-49
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• 2019

This study statistically analyzed the laboratory accidents by investigating 806 laboratory accident survey reports which were officially submitted to government from 2013 to June 2017. After comparing domestic and foreign accident classification criteria, the laboratory accidents were classified using KCD7(Korean Standard Classification of Diseases) and OIICS(Occupational Injury and Illness Classification System) criteria. For the type and part of injury, KCD7 classification criteria was adopted. And, for the cause and occurrence type of accidents, OIICS was adopted to analyze the laboratory accidents. Most of injuries happened to the wrist and hand caused by sharp materials or chemical materials. The analysis of accident cause showed that accidents resulted in medical practice and accidents from handtools and chemical materials such as acid and alkali frequently occurred. The major occurrence types of laboratory accidents was body exposure to the chemical materials such as hydrochloric acid and sulfuric acid. In addition, the accidents resulted in destroy of grasped object or falling object were frequently reported.

• Journal of the Korean Society of Safety
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• v.33 no.6
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• pp.157-162
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• 2018

There have been 1,039 accidents in laboratory(National Research Safety Headquarters). Accidents in laboratory has increased by 71.5% from 158 cases in 2011 to 271 cases in 2016. Accident analysis results show that there has been no death accident after 2011 when 5 death accidents happened. The results also show that severe injuries have been 23 cases(2%) from 2011 to 2016(7 cases in 2011, 2 cases in 2012, 2013 and 2014, 3 cases in 2015, 7 cases in 2016). Minor injuries shows increasing trend from 151 cases in 2011(92.6%) to 294 cases in 2016(97.6%). Among the causes of accidents in laboratory, piercing injuries by injector were 69 cases(10.4%) for recent 3 years, i.e. 22 cases(12.6%) in 2014, 18 cases(14.2%) in 2015 and 29 cases(16%). Piercing injuries by injector with infection such as viral hepatitis and HIV/AIDS were identified in 10 cases in 2014, 5 cases in 2015, and 10 cases in 2016.Therefore, we would like to contribute to the safety of laboratories by suggesting a guideline for prevention and post management of laboratory accidents.

• Journal of Technologic Dentistry
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• v.44 no.3
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• pp.81-88
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• 2022

Purpose: This study was conducted to verify the effects of self-esteem and critical thinking disposition on dental laboratory technology students' field practice stress. Methods: The subjects of the study were 198 dental laboratory technology students with clinical practice experience located in Gyeongsangbuk-do and Gyeongsangnam-do. The collected data were analyzed by t-test, ANOVA, correlation analysis, and linear regression analysis using IBM SPSS Statistics ver. 22.0 (IBM). Results: The average self-esteem, critical thinking disposition, and field practice stress scores were 3.76, 3.50, and 2.40, respectively. There was a significant correlation between self-esteem, critical thinking disposition, and field practice stress. It was found that the higher the self-esteem, the lower was the field practice stress. On the contrary, among the sub-items of critical thinking disposition, when intellectual fairness increased, the field practice stress decreased; however, when healthy skepticism increased, the field practice stress increased. Conclusion: Self-esteem and critical thinking disposition were both found to have a substantial effect on field practice stress. Therefore, various educational programs need to increase self-esteem and cultivate critical thinking skills for a successful field practice and field practice stress relief of laboratory technology students.

• Journal of Veterinary Clinics
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• v.41 no.2
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• pp.88-94
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• 2024

Pet rabbits are affected by the highly contagious ectoparasite Psoroptes (P.) ovis, which carries significant economic implications for the global rabbit industry. Accurate identification of the mite species remains essential to implement effective treatment and control strategies. Two approximately one-year-old female pet rabbits were admitted to the Veterinary Teaching Hospital of Chungbuk National University due to excessive scratching of the ears and the presence of waxy debris within the ear canals. Mites were isolated from the waxy debris extracted from the ear canals and subsequently identified as Psoroptes spp. through microscopic examination. Species confirmation was achieved through mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis. The analysis revealed the mites to be P. ovis based on cox1 gene sequences. The deposited GenBank accession numbers for these sequences are OR985022 and OR985023. This represents the first report of mitochondrial cox1 gene sequences of P. ovis isolated from pet rabbits in South Korea.