• The Korean Journal of Food And Nutrition
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• v.36 no.3
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• pp.202-208
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• 2023

FT-IR, GC/MS, and ATR-FT-IR analyses were performed to confirm the physicochemical characteristics of saw palmetto fruit (SPF) extract. FT-IR analysis of the standard product showed that the band corresponding to the carbonyl bond of free fatty acid was stronger than the band of acyl-glyceride. Sample E was identified as having the same trend as the standard sample. Fatty acid composition analysis revealed that the main fatty acids in the standard sample were lauric acid and oleic acid. The content of lauric acid ranged from approximately 30% to 38% in samples B, C, D, and E, while the content of oleic acid ranged from approximately 29% to 34%. The GC/MS analysis confirmed that the standard SPF extract consisted of fatty acids and fatty acid ethyl esters. Sample E demonstrated a similar pattern to the standard samples in terms of oleic acid, lauric acid, and fatty acid esters. ATR-FT-IR analysis indicated that only sample E was predicted to contain 100% saw palmetto extract. Therefore, these study findings can be considered fundamental data for analyzing the physicochemical characteristics of the composition of SPF extract.

• YAKHAK HOEJI
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• v.52 no.6
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• pp.480-487
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• 2008

We described an estimation of measurement uncertainty in quantitative analysis of 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THCCOOH), the major metabolite of ${\Delta}^9$-tetrahydrocannabinol, in urine sample by solid-phase extraction (SPE) and GC/MS detection. The analytical results were compared and the different contributions to the uncertainty were evaluated. Inter-day and inter-person validation were performed using statistical analysis of several indicative factors. Measurement uncertainty associated with target analyte in real forensic samples was estimated using quality control (QC) data. Traceability of measurement was established through traceable standards, calibrated volumetric glassware and volume measuring device. The major factors of contribution to combined standard uncertainty, were calibration linearity, inter-day repeatability and inter-person reproducibility, while those associated with preparation of analytical standards and sampling volume were not so important considering the degree of contribution. Relative combined standard uncertainties associated with the described method was 12.05% for THCCOOH.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.71-77
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• 2011

Drought and salinity are the most important abiotic stresses that affect the normal growth and development of plants. Glycine betaine is one of the most important osmolytes present in higher plants that enable them to cope with environmental stresses through osmotic adjustment. In this study, a betaine aldehyde dehydrogenase (BADH) gene from spinach under the control of the stress-induced promoter rd29A from Arabidopsis thaliana was introduced into potato cultivar Gannongshu 2 by the Agrobacterium tumefaciens system. Putative transgenic plants were confirmed by Southern blot analysis. Northern hybridization analysis demonstrated that expression of BADH gene was induced by drought and NaCl stress in the transgenic potato plants. The BADH activity in the transgenic potato plants was between 10.8 and 11.7 U. There was a negative relationship (y = -2.2083x + 43.329, r = 0.9495) between BADH activity and the relative electrical conductivity of the transgenic potato plant leaves. Plant height increased by 0.4-0.9 cm and fresh weight per plant increased by 17-29% for the transgenic potato plants under NaCl and polyethylene glycol stresses compared with the control potato plants. These results indicated that the ability of transgenic plants to tolerate drought and salt was increased when their BADH activity was increased.

• Nuclear Engineering and Technology
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• v.53 no.3
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• pp.763-775
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• 2021

The thermal hydraulic performances of Steam Generator (SG) under both steady and transient operation conditions are of great importance for the safety and economy in nuclear power plants. In this paper, based on our self-developed SG thermal hydraulic analysis code STAF (Steam-generator Thermalhydraulic Analysis code based on Fluent), an improved new version STAF-CT (fully Coupling and Transient) is developed and introduced. Compared with original STAF, the new version code STAF-CT has two main functional improvements including "Transient" and "Fully Three Dimensional Coupling" features. In STAF-CT, a three dimensional energy transferring module is established which can achieve energy exchange computing function at the corresponding position between two sides of SG. The STAF-CT is validated against the international benchmark experiment data and the results show great agreement. Then the U-shaped SG in AP1000 nuclear power plant is modeled and simulated using STAF-CT. The results show that three dimensional flow fields in the primary side make significant effect on the energy source distribution between two sides. The development of code STAF-CT in this paper can provide an effective method for further SG high fidelity research in the nuclear reactor system.

• Steel and Composite Structures
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• v.25 no.3
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• pp.257-270
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• 2017

In this article, an efficient and simple refined theory is proposed for buckling analysis of functionally graded plates by using a new displacement field which includes undetermined integral variables. This theory contains only four unknowns, with is even less than the first shear deformation theory (FSDT). Governing equations are obtained from the principle of virtual works. The closed-form solutions of rectangular plates are determined. Comparison studies are carried out to check the validity of obtained results. The influences of loading conditions and variations of power of functionally graded material, modulus ratio, aspect ratio, and thickness ratio on the critical buckling load of functionally graded plates are examined and discussed.

• Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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• 2001.02a
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• pp.53-56
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• 2001

The stability of PF 6-7 has been studied according to the transient analysis code TOKSCPF and quench analysis code QSAIT. We compare the stability and temperature rise with various Cu/SC ratios of 2.8 and 3.5 under the KSTAR normal operating conditions. It shows that the Cu/SC ratio has an influence on the quench propagation and stability margin. In transient operating condition, the Cu/SC ratio weakly influences on the temperature rise in PF magnet.

• Korean Journal of Clinical Laboratory Science
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• v.40 no.1
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• pp.18-25
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• 2008

In classical titrimetric analyses, the major concern is the concentration of titrant, usually the aqueous solution of hydrochloric acid or sodium hydroxide, that could be changed as time goes by and it is accompanied with the inaccuracy of the resulting data. And the statistical approach, the nonlinear regression analysis, which is a well-known statistical method, was introduced to determine the accurate concentration of the titrant and the exact value of parameters, $K_a$, r, $C_a$, $C_b$, for 0.01 M aqueous solutions of analytes, sodium pyruvate, sodium acetate, sodium bicarbonate, ammonium hydroxide, ammonium chloride and acetic acid at $25^{\circ}C$. We used Gauss-Newton method for the linearlization of the nonlinear titration system and the two-parameter fitting showed appreciable convergent data for the parameters of the analytes set with the various range of $K_a$ value.

• Smart Structures and Systems
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• v.1 no.3
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• pp.257-266
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• 2005

In Lamb wave detection of damages in smart structures, the excitation pulse is usually designed as a narrow band burst wave for the convenience of analysis and recognition. However, the wideband excitation can excite more modes in plate/shell structure and thus provides extra information for changes of the structure. This paper presents a method that can extract information in wideband Lamb wave signals. By transforming the detected signals into various sub-frequency band, the measured signal can be converted to its equivalences of narrow band excitations, therefore, the information in different frequency bands can be acquired from a single test and in the same time the complicity of wideband signal can be simplified. Some test results are provided to verify this method.

• Molecules and Cells
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• v.26 no.1
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• pp.87-92
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• 2008

We employed dual color Fluorescence Cross Correlation Spectroscopy (FCCS) to measure the interaction between PKA regulatory (RII) and catalytic subunits (CAT) in living cells. Elevation of intracellular cAMP with forskolin decreased the cross-correlation amplitude between RFP-fused RII (RII -mRFP) and GFP-fused CAT (CAT-EGFP) by 50%, indicating that cAMP elevation leads to dissociation of RII-CAT complexes. Moreover, diffusion coefficient analysis showed that the diffusion rate of CAT-EGFP was significantly increased, suggesting that the decreased RII-CAT association caused by cAMP generated free CAT subunits. Our study demonstrates that in vivo FCCS measurements and their quantitative analysis permit one not only to directly quantify protein-protein interactions but also to estimate changes in the intracellular cAMP concentration.

• Korean Journal of Clinical Laboratory Science
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• v.42 no.3
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• pp.111-115
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• 2010

A 15-year-old female with primary amenorrhea and Tuner's syndrome feature was referred for a chromosome analysis. The karyotype of the patient was 45,X/46,X,der(Y) mosaicism under initial GTG-banding analysis. Fluorescence in situ hybridization (FISH) analysis with probe for CEP X probes and SRY probe (Vysis, Inc. Downers Grove, IL 60515, USA) was carried out. This probe is direct labeled with SpectrumOrange (SRY, Yp11.3) and is available as a single probe or mixed with the CEP X SpectrumGreen probe. SRY SpectrumOrange/CEP X SpectrumGreen hybridized to a specimen obtained from an two isodicentric Y chromosomes. The karyotype of the patient was ish Xcen(DXZ1x1)/Xcen(DXZ1x1), Yp11.3(SRYx2) by using FISH. This karyotype was considered a variant of Tuner syndrome with mixed gonadal dysgenesis (MGD), male pseudohermaphroitism (MPH) and apparently normal male.