• Title/Summary/Keyword: laboratory analysis

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Characteristic Studies of Plasma Treated unidirectional Hildegardia Populifolia Fabric

  • Prasad, C. Venkata;Lee, D.W.;Sudhakara, P.;Jagadeesh, D.;Kim, B.S.;Bae, S.I.;Song, J.I.
    • Composites Research
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    • v.26 no.1
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    • pp.54-59
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    • 2013
  • This study deals with effect of plasma treatment on the properties of unidirectional ligno cellulosic fabric Hildegardia Populofolia (HDP) fabric. Thermal stability of the fabric was determined by differential scanning calorimetry (DSC) and Thermo gravimetric analysis (DSC). Morphological properties was analyzed by SEM analysis and found that the surface was rough upon plasma treatment which provides good interfacial adhesion with matrix during composite fabrication. Thermal stability and mechanical properties of the plasma treated fabric slightly increases compare to alkali and untreated fabric. It was observed that tensile properties of the fabric increases upon plasma treatment due to the formation of rough surface. SEM analysis indicates formation of rough surface on plasma treatment which helps in increasing the interfacial interaction between the matrix (hydrophobic) and fabric (hydrophilic).

Analysis of Cytogenetics Diagnosis: form 30 Years Experience at Kwangju Christian Hospital (광주기독병원 30년간 세포유전학적 검사결과의 고찰)

  • Kim, Yoon Sik;Kee, Won Jin;Back, Hae Kyong;Lee, Sang Ho;Shin, Jin Ho
    • Korean Journal of Clinical Laboratory Science
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    • v.36 no.2
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    • pp.205-209
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    • 2004
  • This study was carried out to review and evaluate a total of 2,463 cases of human chromosomal analysis at Kwangju Christian Hospital from 1974 to 2004. We collected 2.0-3.0ml of human peripheral blood in heparized bottle. Then, we cultured it for 72 hours. We performed GTG-banding and chromosomal kayotyping analysis by Cytovision kayotyping system. Abnormal karyotypes were observed in 30.5% of the total cases (750/2,463). Autosome and sex chromosome anomalies were observed in 25.8% (635/2,463) and 4.7% (115/2,463) respectively. In a total of 2463 cases, there were 522 (22.4%) cases of Down's syndrome karyotype, and 67 (2.7%) cases of Turner syndrome. In conclusion, Down's syndrome has decreased after the end of the 1990s, but other (Turner syndrome et al.) chromosomal abnormal cases haven't decreased after the1970s.

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Comparison of the Cross-Sectional Area of Longus Colli and Muscle Activity of Sternocleidomastoid in Subjects With Forward Head Posture on the Two Craniocervical Flexion Methods

  • Son, Min-chan;Hwang, Ui-jae;Jung, Sung-hoon;Ahn, Sun-hee;Kim, Hyun-a;Kwon, Oh-yun
    • Physical Therapy Korea
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    • v.25 no.2
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    • pp.62-70
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    • 2018
  • Background: The craniocervical flexion (CCF) exercise is one of the effective exercise in correcting forward head posture (FHP). However, some people with FHP achieve CCF with compensatory movements, for example, low cervical flexion using superficial neck flexors such as the sternocleidomastoid (SCM) muscle. No study has yet investigated whether a dual­pres ure biofeedback unit (D-PBU) method to prevent low cervical flexion would be helpful in performing pure CCF movement. Objects: The purpose of this study was to compare the effects of the CCF using D-PBU method and the traditional CCF method on the cross-sectional area (CSA) of the longus colli muscle (LCM) and the activity of SCM muscle in subjects with FHP. Methods: Twenty­four FHP subjects (male: 16, female: 8) were recruited for this study. All subjects performed CCF using two different methods: the traditional CCF method and the CCF using D-PBU method. The CSA of the LCM was measured via ultrasound, and surface electromyography was used to measure SCM muscle activity. Results: The change in CSA of the LCM was significantly larger during the CCF using D-PBU method ($1.28{\pm}.09$) compared with the traditional CCF method ($1.19{\pm}.08$) (p<.05). The SCM muscle activity using the CCF using D-PBU method ($2.01{\pm}1.97$ %MVIC) was significantly lower than when using the traditional CCF method ($2.79{\pm}2.32$ %MVIC) (p<.05). Conclusion: The CCF using D-PBU method can be recommended for increasing LCM activation and decreasing SCM muscle activity during CCF movement in subjects with FHP.

DNA Polymorphism in SLC11A1 Gene and its Association with Brucellosis Resistance in Indian Zebu (Bos indicus) and Crossbred (Bos indicus×Bos taurus) Cattle

  • Kumar, Nishant;Ganguly, Indrajit;Singh, Rajendra;Deb, Sitangsu M.;Kumar, Subodh;Sharma, Arjava;Mitra, Abhijit
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.7
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    • pp.898-904
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    • 2011
  • The PCR- restriction fragment length polymorphism (RFLP) in and around TM4 of SLC11A1 gene and its association with the incidences of brucellosis in Hariana breed (Bos indicus) and Holstein Friesian crossbred (Bos indicus${\times}$Bos taurus) cattle was examined. A fragment of 954 bp encoding the TM4 was amplified, and RFLP was identified by digestion of the amplicon independently with AluI and TaqI. The amplicon (GenBank Acc. No. AY338470 and AY338471) comprised of a part of exon V (<59 bp) and VII (62>), and entire intron 5 (423 bp), exon VI (71 bp) and intron 6 (339 bp). Digestion with AluI revealed the presence of two alleles viz, A (281, 255, 79 and 51 bp) and B (541, 255, 79 and 51 bp). The frequency of A allele was estimated as 0.80 and 0.73 in Hariana and crossbred cattle, respectively. Due to presence of a polymorphic TaqI site at intron 5, two alleles: T (552 and 402 bp) and Q (231, 321 and 402 bp) were identified. The frequency of T allele was estimated as 0.96 and 0.97, respectively. For association study, on the basis of serological tests and history of abortion, the animals were grouped into "affected" and "non-affected". However, no association could be established with the observed RFLPs.

Assessment of the crest cracks of the Pubugou rockfill dam based on parameters back analysis

  • Zhou, Wei;Li, Shao-Lin;Ma, Gang;Chang, Xiao-Lin;Cheng, Yong-Gang;Ma, Xing
    • Geomechanics and Engineering
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    • v.11 no.4
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    • pp.571-585
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    • 2016
  • The crest of the Pubugou central core rockfill dam (CCRD) cracked in the first and second impounding periods. To evaluate the safety of the Pubugou CCRD, an inversion analysis of the constitutive model parameters for rockfill materials is performed based on the in situ deformation monitoring data. The aim of this work is to truly reflect the deformation state of the Pubugou CCRD and determine the causes of the dam crest cracks. A novel real-coded genetic algorithm based upon the differences in gene fragments (DGFX) is proposed. It is used in combination with the radial based function neural network (RBFNN) to perform the parameters back analysis. The simulated settlements show good agreements with the monitoring data, illustrating that the back analysis is reasonable and accurate. Furthermore, the deformation gradient of the dam crest has been analysed. The dam crest has a great possibility of cracking due to the uncoordinated deformation, which agrees well with the field investigation. The deformation gradient decreases to the value lower than the critical one and reaches a stable state after the second full reservoir.

Matrix Metalloproteinase-9 as a Prognostic Factor in Gastric Cancer: A Meta-Analysis

  • Zhang, Qiong-Wen;Liu, Lei;Chen, Ru;Wei, Yu-Quan;Li, Ping;Shi, Hua-Shan;Zhao, Yu-Wei
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.6
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    • pp.2903-2908
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    • 2012
  • Background: Matrix metalloproteinase-9 (MMP-9) is associated with disruption of basement membranes of blood vessels and promotion of metastasis through the lymphatics. However, its prognostic value for survival in patients with gastric cancer remains controversial. Method: We therefore conducted a meta-analysis of the published literature in order to clarify the impact of MMP-9. Clinical studies were selected for further analysis if they provided an independent assessment of MMP-9 in gastric cancer and reported analysis of survival data according to MMP-9 expression. Results: A total of 11 studies, covering 1700 patients, were included for meta-analysis. A summary hazard ratio (HR) of all studies and sub-group hazard ratios were calculated. The combined HR suggested that a positive MMP-9 expression had an impact on overall survival: 1.25 (95% confidence interval 1.11-1.40) in all eligible studies; 1.13 (1.06-1.20) in 8 studies detecting MMP-9 by immunohistochemistry; 1.36 (1.12-1.65) in 7 studies from Asia. Only one study for DFS showed a significant impact on disease free survival (HR 1.73, 95%CI 1.27-2.34). Conclusions: Our findings suggested that MMP-9 protein expression might be a factor for a poor prognosis in patients with gastric cancer. However, the association was rather weak, so that more prospective studies should further explore the prognostic impact of MMP-9 mRNA and correlations between MMP-9 and clinicopathological characteristics.