• Title/Summary/Keyword: laboratory

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Assessment of allergenicity of genetically modified foods (GMOs)

  • Lee, Jung-Hyun;Yoon, Won-Ki;Han, Sang-Bae;Yun, Si-On;Park, Sun-Hong;Lee, Hyun-Ju;Yoon, Pyung-Seop;Moon, Jae-Sun;Kim, Hyung-Chin;Kim, Hwan-Mook
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.267.1-267.1
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    • 2002
  • The potential allergenicity of the transgene products in genetically modified organisms (GMOs). has been an important issue. As a part of the risk assessment of GMOs. we investigated the physicochemical stability and the immunogenicity of food allergens to determine their allergenicity. We have systematically evaluated the stability of food allergens in the gastrointestinal tract by using simple models of gastric (Stimulated gastric fluid) and intestinal (Stimulated intestinal fluid) digestion. (omitted)

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Biological Parameters of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Fed on Rabbits, Sheep, and Cattle

  • Ma, Miling;Chen, Ze;Liu, Aihong;Ren, Qiaoyun;Liu, Junlong;Liu, Zhijie;Li, Youquan;Yin, Hong;Guan, Guiquan;Luo, Jianxun
    • Parasites, Hosts and Diseases
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    • v.54 no.3
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    • pp.301-305
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    • 2016
  • In order to determine the effect of various hosts on feeding performance of Rhipicephalus (Boophilus) microplus, we used 3 mammalian species as hosts, cattle (Qinchuan), sheep (Tan), and rabbits (Japanese white rabbit) for infesting ticks. Five hundreds of R. microplus larvae were exposed to each animal (3 animals/host species). Tick recoveries were 11.0%, 0.47%, and 5.5% from cattle, sheep, and rabbits, respectively. The averages of tick feeding periods were not significantly different on cattle, sheep, and rabbits, 28.8, 25.3, and 26.7 days, respectively. The average weights of individual engorged female from cattle, sheep, and rabbits were 312.5, 219.1, and 130.2 mg, respectively and those of egg mass weights each to 85.0, 96.6, and 17.8 mg. The highest egg hatching rate was in the ticks from cattle (96.0%), followed by those from rabbits (83.0%) and sheep (19.2%). These data suggest that rabbits could be as an alternative host to cultivate R. microplus for evaluating vaccines and chemical and biological medicines against the tick in the laboratory, although the biological parameters of ticks were less than those from cattle.

Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1

  • Li, Wen Hui;Jia, Wan Zhong;Qu, Zi Gang;Xie, Zhi Zhou;Luo, Jian Xun;Yin, Hong;Sun, Xiao Lin;Blaga, Radu;Fu, Bao Quan
    • Parasites, Hosts and Diseases
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    • v.51 no.2
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    • pp.197-201
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    • 2013
  • A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.

An Analysis of Productivity according to the Actual Conditions of Dental Laboratory Technician (치과기공사의 업무실태에 따른 생산성 분석 연구)

  • Cha, Sung-Soo
    • Journal of Technologic Dentistry
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    • v.9 no.1
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    • pp.5-19
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    • 1987
  • The factors which impact on the productivity of medical organization are technique, manager, raw materials, management style, manpower structure, job construction, and so on. Human resources is the most important factor among them, but the productivity of serice organization can not be measured easily. The purpose of this study was to verify the effect of laboratory status, the degree of job cognition, and stress on the productivity of dantal prosthetic products, and to fine out the factors which impact on the productivity. The results were as follows: 1. The job characteristice of crown bridge laboratory part and porcelain laboratory part similar to each other. Also partial denture laboratory part and complete denture laboratory part were similar to each other. But that of orthodontic laboratory part was different from other. 2. the degree of job cognition made significant difference statisfically according to job characteristics of each dental laboratorise. 3. The correlation coefficient between job cognition of dental laboratory technician and workshop environment was.23, but the correlation between job cognition job stress appeared reversely, its coefficient was-.17. 4. The productivity of technician engaged in dental laboratory was much heigher than that of technician engaged in dental clinical laboratory room, and each were different in the degree of job cognition. 5. the most important factors which impact one the performance productivity of dental laboratory technician were the fact whether he married or not and his status.

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System Design for the Implementation of Virtual Laboratory (가상 실험실 구현을 위한 시스템 설계)

  • Song Min-Gyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.6 no.6
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    • pp.547-554
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    • 2005
  • Virtual Laboratory is the new concept in the field of Control & Measurement as network technology development, it is used in many university and institute nowadays. It is possible to control and measure the instrument in anywhere, anytime by the use of Virtual Laboratory and we can raise the efficiency of experiment. In this paper, we review the concept and necessity of Virtual Laboratory at first and discuss about how we can implement Virtual Laboratory. Then we design framework of Client-Server based Virtual Laboratory fur operating of remote instrument through network, and finally we suggest system model of Virtual Laboratory capable of controlling & monitoring of instrument.

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Some Universal Characteristics of Intertidal Bacterial Diversity as Revealed by 16S rRNA Gene-Based PCR Clone Analysis

  • Shuang, J.L.;Liu, C.H.;An, S.Q.;Xing, Y.;Zheng, G.Q.;Shen, Y.F.
    • Journal of Microbiology and Biotechnology
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    • v.16 no.12
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    • pp.1882-1889
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    • 2006
  • A 16S rDNA clone library was generated to investigate the bacterial diversity in intertidal sediment from the coast of the Yellow Sea, P. R. China. A total of 102 clones were sequenced and grouped into 73 OTUs using a phylogenetic approach. The sequenced clones fell into 11 bacterial lineages: Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Spirochaetes, and candidate divisions of BRCl, OP3, and OP1l. Based on a phylogenetic analysis of these bacteria, together with the ten most closely related sequences deposited in the GenBank, it was concluded that intertidal bacteria are most likely derived from marine bacteria with a remarkable diversity, and some are particularly abundant in intertidal sediment.

Review on the Aims of Laboratory Activities in School Science (학교 과학수업에서 실험의 목적에 대한 고찰)

  • Yang Il-Ho;Cho Hyun-Jun
    • Journal of Korean Elementary Science Education
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    • v.24 no.3
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    • pp.268-280
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    • 2005
  • Teaching with laboratory activities in school science, which are distinctive characteristic, is placed from other disciplines fur teaming almost 200 hundred years ago. A number of science educators have suggested that there are rich benefits in teaming from using laboratory activities. At these time, however, some educators have begun to seriously question the effectiveness and the role of laboratory activities. There are some causes related to obscure and vague aims of laboratory activities. The purposes of this paper is to review aims of laboratory activities presented in the literatures through historical overview, and to obtain implication for school science. There are various aims of laboratory activities by a number of researchers. Overall synthesizing, there are ffur domains of aims of science teaching through laboratory activities, (a) science knowledge has two sub-domains; scientific content knowledge and procedural knowledge, (b) nature of science, (c) science attitude has two sub-domain; scientific attitude and attitude toward science, and (d) ability of scientific inquiry has two sub-domain; manipulative skills and scientific thinking. But, it is necessary to continue the following study in order to obtain the aims of laboratory activities agreed by expert community, and setting up of lists of aims of laboratory activities for students to achieve hierarchies of school science curriculums.

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Impedance Calculation for Vacuum Components in Pohang Light Source Storage Ring

  • Ju, Yeong-Do;Lee, Byeong-Jun;Son, Yeong-Uk;Yu, In-Ha;Jeon, Myeong-Hwan;Park, In-Su;Kim, Seung-Hwan;Ha, Tae-Gyun;Gong, Hyeong-Seop;Son, Yun-Gyu;Park, Yong-Jeong;Park, Jong-Do;Nam, Sang-Hun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.151.2-151.2
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    • 2014
  • Broadband impedances for the 3-GeV pohang light source-II (PLS-II) storage ring have been numerically estimated using a full three dimensional finite-difference time-domain code, CST particle studio. The total broadband impedance of all the vacuum components was estimated as 0.256 ohm, which is a small fraction of the total machine impedance budget.

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