• Journal of Microbiology and Biotechnology
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• v.20 no.9
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• pp.1322-1330
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• 2010

Three types of nuruk were made from rice, wheat, and a rice-glasswort (6:4) mixture. Nuruk, makgeolli, and vinegar were manufactured with rice nuruk (RN), wheat nuruk (WN), and rice-glasswort nuruk (RGN). The variable region of 18S or 16S rDNA amplified with genomic DNA extracted directly from nuruk-, makgeolli-, and vinegar-making cultures was analyzed via temperature gradient gel electrophoresis (TGGE). The sequence of the 18S rDNA variable region extracted from the TGGE gel for nuruk was 99% homologous with Aspergillus sp. and that for the makgeolli-making culture was 99% homologous with Saccharomyces sp. and Saccharomycodes sp. The sequence of the 16S rDNA variable region extracted from TGGE gel for the vinegar-making culture was 98% homologous, primarily with the Acetobacter sp. The eukaryotic and prokaryotic diversities in the nuruk-, makgeolli-, and vinegar-making cultures was not significantly altered by the addition of glasswort. Prokaryotic diversity was higher than eukaryotic diversity in the nuruk, but eukaryotic diversity was higher than prokaryotic diversity in the makgeolli-making culture, on the basis of the TGGE patterns. No 18S rDNA was amplified from the DNA extracted from the vinegar-making culture. The diversity of the microbial community in the process from nuruk to vinegar was slightly affected by the type of raw material utilized for nuruk-making. The saccharifying activity and ethanol productivity of nuruk, polyphenol content in makgeolli, and acetic acid and polyphenol content in the vinegar were increased as a result of the addition of glasswort. In conclusion, the glasswort may be not simply an activator for the growth of microorganisms during the fermentation of nuruk, makgeolli, or vinegar, but also a nutritional supplement that improves the quality of vinegar.

• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.322-330
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• 2016

Understanding the genetic variation among landrace collections is important for crop improvement and utilization of valuable genetic resources. The present study was carried out to analyse the genetic diversity and associated population structure of 621 foxtail millet accessions of Korean landraces using 22 EST-SSR markers. A total of 121 alleles were detected from all accessions with an average of 5.5 alleles per microsatellite locus. The average values of gene diversity, polymorphism information content, and expected heterozygosity were 0.518, 0.594, and 0.034, respectively. Following the unweighted neighbor-joining method with arithmetic mean based clustering using binary data of polymorphic markers, the genotypes were grouped into 3 clusters, and population structure analysis also separated into 3 populations. Principal coordinate analysis (PCoA) explained a variation of 13.88% and 10.99% by first and second coordinates, respectively. However, in PCoA analysis, clear population-level clusters could not be found. This pattern of distribution might be the result of gene flow via germplasm exchanges in nearby regions. The results indicate that these Korean landraces of foxtail millet exhibit a moderate level of diversity. This study demonstrated that molecular marker strategies could contribute to a better understanding of the genetic structure in foxtail millet germplasm, and provides potentially useful information for developing conservation and breeding strategies.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.105-105
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• 2018

Finger millet, Eleusine coracana Gaertn., is more nutritious than other cereals and millets and widely cultivate in tropical regions of the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In recent years, microsatellites have become the most used markers for studying population genetic diversity. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers using a total of 1,927 ESTs of Eleusine coracana available in the NCBI database. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions was classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

• The Korean Journal of Mycology
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• v.41 no.3
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• pp.137-141
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• 2013

Diversity of endophytic fungi of two species associated with host plants, Pinus densiflora and Larix kaempferi in Mt. Oser were investigated. Endophytic fungi were isolated from surface sterilized leaves of the 13 host plants and identified by morphological traits and rDNA ITS sequences analysis. Totally 37 isolates were discovered and identified into 17 taxa. Of them, 59% isolates belonged to Leotiomycetes, 30% isolates Sordariomyetes, 8% isolates Dothideomycetes and 3% isolates Agaricomycetes. Results showed a similar diversity pattern with previous studies. Also, species diversity (H') of endophytic fungi isolated from L. kaempferi was higher than that from P. densiflora. The endophytic species composition showed significant differences between host plants. Particularly, taxon of Lophodermium is a major genus in the host plants. However, critical research of the endophytic species diversities within the genus in Korea is required. The communities of endophytic fungi isolated in this study showed differences in diversities and species composition between host plants.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.17 no.11
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• pp.1320-1331
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• 1992

The error rate equation of FH/MFSK signal of repeated diversity received over m-distribution fading channel with partial-band interference has been derived and the error rate has been evaluated to show in figures as a function of signal to noise ratio, signal to interference ratio, fading figure, repeated number, partial-band interference fraction and power correlation coefficient between signal and interference. In this paper, we assumed that repeated diversity technique is used in transmitter against fading occurred in mobile radio channel. By comparing the error rate performance in diversity against fading and the obtainable degree of improvement of error performance with diversity technique has been found out. From the result, it is known that error rate performance deteriorates more as $\rho$ becomes small i.e., the partial-band interference gives more effect on error performance than full-band interference. Also when the values of $E_b/N_1$ and $\rho$ are small. as depth of fading diversity technique.

• Journal of Nutrition and Health
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• v.30 no.7
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• pp.854-869
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• 1997

This study was conducted to evaluation the quality of meals served in elementary school based on nutrient contents and food diversity . A questionnaire was mailed to school foodservice dietitians requesting one week menus for April and October . Menus served in 388 schools from each province and from major cities in Korea were analyzed. Mean nutrient contents per meal of persons served, and food expenses per meal. Mean percentages of energy from fat was 20.2% , and was significantly l different by foodservice system. When nutrient contents were converted to amount per 1, 000kcal, all were higher than that calculated from RDA except calcium, iron, and Vitamin A. Most of these were not significantly different according to area, foodservice system, the number of persons served, and food expense per meal. Compared to 1/3 of RDA for children , many meals provided lower amounts of energy, Vit A, VitB2, and Ca. More than half of the meals provided less iron than 1/3 RDA for 10-12 years girls. Using the highest values of RDA for the elementary school age group as evaluation, criteria nutrient adequacy ratios(NAR) were lowest for Ca(0.61), Vit A(0.57), and iron(0.77). The mean adequacy ratio(MAR) was 0.86. Mean numbers of food items and dishes per meal were 15.3 and 5.1 , respectively. 66.3% of the meals provided three food groups of grain, meat, and vegetable. Except for the number of different food items served per meal, the aspect of food diversity was similar to that of nutrient contents. There was significant positive correlation between mean number of different food items served per meal and nutrient content . In summary , schools in small cities and rural areas than those in large cities, schools adapting commissary or joint management than those or density. Also the more food groups served per meal, the higher the nutrient content per meal . In summary , schools in small cities and rural areas than those in large cities, schools adapting commissary or joint management than those adapting conventional foodservice system, schools serving smaller number of persons, and schools with higher food expenses provided higher energy contents and food diversity.

• Journal of Veterinary Science
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• v.22 no.4
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• pp.50.1-50.10
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• 2021

Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions: Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.

• Animal Systematics, Evolution and Diversity
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• v.2 no.2
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• pp.1-12
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• 1986

Genetic and morphological variation among seven populations of two species of the genus Liobagrus that had been collected from six separate river systems in Korea were analyzed by means of starch electrophoreiss and discriminant function analysis . The degree of genic variation among three population of L.andersoni was P=21.3%, H=0.068 , and that of four population of L.mediadiposalis was P=16.3%, H=0.560. Rogers' gentic similarity coefficient (S) of L.andersoni and L.mediadiposalis were S=0.592 , S=0.675 respectively. In morphometric characters of L. andersoni, the population from Kum River was distinctly different from other conspecific populations.

• Korean Journal of Environment and Ecology
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• v.12 no.3
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• pp.290-296
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• 1998

Altitudinal changes in the vegetation of Sunginbong(984m) in UIlungdo (Island) were investigated by sample plots(forty l00$\m^2$) along elevation and analyzed by TWINSPAN and DCA techniques. In the results from the analysis of both techniques, altitudinal zonations were divided into 3 groups such as lower 300m area, 400~700m area, and above 800m area. DCA and similarity index analyses of elevational ranges showed discontinuities between lower and middle elevation areas. But there was a vegetation continuum between middle and high elevation areas. In the analysis of species diversity, there was no significant difference due to altitude except for maximum species diversity which was decreased with altitude. The mean tree density of canopy and understory layers in the middle elevation area showed the highest value, and the highest basal area was recorded at the lower elevation area.

• ALGAE
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• v.32 no.3
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• pp.171-179
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• 2017

The strictly freshwater red algal order Batrachospermales has undergone numerous taxonomic rearrangements in the recent past to rectify the paraphyly of its largest genus Batrachospermum. These systematic investigations have led to the description of new genera and species as well as re-circumscription of some taxa. Specimens collected from two locations in the southeastern USA were initially identified as being allied to Batrachospermum sensu lato, but could not be assigned to any recognized species. Representative rbcL (plastid) and COI-5P (mitochondrion) sequences showed these specimens to be similar to each other and not closely matching the previously published sequence data for other Batrachospermum taxa. Comparison of sequence variation and morphology with a broader range of batrachospermalean taxa resulted in the proposal of a new monotypic genus Lympha mucosa gen. et sp. nov. to accommodate these specimens. Lympha mucosa is sister to members of a newly described genus Volatus, but the two genera are easily distinguished based on straight versus curved, twisted or spirally coiled carpogonial branch, respectively. This new taxon has morphological similarities to Batrachospermum sections Turfosa and Virescentia, but can be differentiated based on genetic divergence in rbcL and COI-5P as well as a combination of morphological characters: dense, compressed whorls, axial carposporophytes with a single type of gonimoblast filament; cortication of the main axis closely appressed; and short, straight carpogonial branch arising from the pericentral cell and carpogonia with unstalked, lanceolate trichogynes. This new taxon adds to the freshwater red algal diversity of the southeastern USA, a region already known for biodiversity and high endemism of the aquatic flora and fauna. It is also a relevant new addition to the taxonomic knowledge of the freshwater red algal Batrachospermales.