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Mucilage Separation of Korean Yam Using Microparticulation/Air Classification Process (초미세분쇄/공기분급을 이용한 마의 점질물 분리)

  • Lee, Boo-Yong;Park, Dong-June;Ku, Kyung-Hyung;Kim, Hyun-Ku;Mok, Chul-Kyoon
    • Korean Journal of Food Science and Technology
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    • v.26 no.5
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    • pp.596-602
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    • 1994
  • To separate and concentrate mucilage from yam(Dioscorea batatas DECNE), yam was dried, microparticulated using impact mill and air-classified at different air classifying wheel speed(ACWS) in classifier. As ACWS increased from 5,000 rpm to 22,500 rpm, the contents of dietary fiber, protein and lipid of air classified microparticles(ACM) increased remarkably. Especially the ACM with ACWS over 15,000 rpm showed 36.41% dietary fiber and 16.66% protein. The dietary fiber and protein components were concentrated to $2.5{\sim}9.0$ times as compared with whole yam powder. Concomitantly the non-fibrous carbohydrate decreased from 88.31% to 16.84. The damaged starch(%), WSI and WAI of ACM of ACWS over 15,000 rpm were $1.5{\sim}3.0$ times higher than those of ACM under ACWS 15,000 rpm. The apparent viscosity of ACM was 0.0800 Pa s over ACWS 15,000 rpm and 0.0080 Pa s under ACWS 15,000 rpm. Judging from viscosity of ACM, the mucilage component of yam was concentrated to 10 times. In conclusion, the optimum process to separate and concentrate the mucilage from yam consisted of the microparticulation to $5{\sim}30{\mu}m$ and the air-classification at ACWS over 15,000 rpm.

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The Study on the Methylmercury Analysis and the Monitoring of Total Mercury and Methylmercury in Fish (어류 중 메틸수은 분석법 확립 및 모니터링)

  • Kim, Hee-Yun;Chung, So-Young;Sho, You-Sub;Oh, Geum-Soon;Park, Seong-Soo;Suh, Jung-Hyuk;Lee, Eun-Ju;Lee, Yoon-Dong;Choi, Woo-Jeong;Eom, Ji-Yoon;Song, Min-Soo;Lee, Jong-Ok;Woo, Gun-Jo
    • Korean Journal of Food Science and Technology
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    • v.37 no.6
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    • pp.882-888
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    • 2005
  • Procedure for analysis of methylmercury in fish was developed, involving addition of HCl, extraction with toluene, and clean-up using L-cystein solution. Obtained extract is analyzed by gas chromatography with electron capture detector using Ulbon HR-Thermon-Hg column. Detection limit and recovery of the method were 0.005mg/kg (expressed as Hg), 98-107 (103%), respectively. Total mercury and methylmercury concentrations in 175 commercial fish samples ranged from [mean-max (mean), unit: mg/kg]: 0.014-1.200 (0.270) and 0.006-0.901 (0.168) in tuna-fish, 0.020-0.934 (0.323) and 0.012-0.553 (0.149) in martin-fish, 0.082-0.782 (0.391) and 0.040-0.436(0.201) in shark, 0,023-0.031 (0.026) and 0,013-0.018 (0.015) in salmon, 0.098-0.193 (0.133) and 0.031-0.015(0.090) in tilefish, and 0,031-0.214 (0.089) and 0.016-0.093 (0.042) in canned tuna respectively. No sample of analyzed fish exceeded 1.0mg/kg wet wt., limit for methylmercury established by Codex. In all species examined, estimated weekly intake was lower than Provisional Tolerable Weekly Intake recommended by the JECFA (the Joint FAO/WHO Expert Committee on Food Additives).

Relationship between iron deficiency anemia and febrile convulsion in infants (영아에서 철결핍 빈혈과 열성경련과의 상관관계)

  • Jun, Youn Soo;Bang, Ho Il;Yu, Seung Taek;Shin, Sae Ron;Choi, Du Young
    • Clinical and Experimental Pediatrics
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    • v.53 no.3
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    • pp.392-396
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    • 2010
  • Purpose : The association between iron deficiency anemia and febrile convulsion in infants has been examined in several studies with conflicting results. Therefore, the authors aimed to evaluate the precise relationship involved. Methods : In this case-control study, the authors assessed 100 children with a diagnosis of febrile convulsion, aged between 9 months and 2 years, during January 2007 to July 2009. The control group consisted of 100 febrile children without convulsion; controls were closely matched to the cases by age, gender, and underlying disease. Results : The mean ages of the febrile convulsion and control group were $16.3{\pm}7.4$ and $15.8{\pm}6.1$ months, respectively, and the two groups had no differences in clinical features. Iron deficiency anemia (Hb <10.5 gm/dL) was more frequent in the febrile convulsion group than in the control group, although there was no statistical significance. Unexpectably, the RDW (red blood cell distribution width) was significantly lower and the MCNC (mean corpuscular hemoglobin concentration) was significantly higher among seizure cases than among the controls (P <0.05). There is no statistical difference between simple and complex febrile groups in the clinical and laboratory profiles. On multiple logistic regression analysis, iron deficiency anemia was more frequent, but the RDW was lower, among the cases with febrile convulsion, compared with the controls. Conclusions : Our study suggests that the iron deficiency anemia is associated with febrile convulsion, and screening for iron deficiency anemia should be considered in children with febrile convulsions.

Clinical Study of Acute and Chronic Pain by the Application of Magnetic Resonance Analyser $I_{TM}$ (자기공명분석기를 이용한 통증관리)

  • Park, Wook;Jin, Hee-Cheol;Cho, Myun-Hyun;Yoon, Suk-Jun;Lee, Jin-Seung;Lee, Jeong-Seok;Choi, Surk-Hwan;Kim, Sung-Yell
    • The Korean Journal of Pain
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    • v.6 no.2
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    • pp.192-198
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    • 1993
  • In 1984, a magnetic resonance spectrometer(magnetic resonance analyser, MRA $I_{TM}$) was developed by Sigrid Lipsett and Ronald J. Weinstock in the USA, Biomedical applications of the spectrometer have been examined by Dr. Hoang Van Duc(pathologist, USC), and Nakamura, et al(Japan). From their theoretical views, the biophysical functions of this machine are to analyse and synthesize a healthy tissue and organ resonance pattern, and to detect and correct an abnormal tissue and organ resonance pattern. All of the above functions are based on Quantum physics. The healthy tissue and organ resonance patterns are predetermined as standard magnetic resonance patterns by digitizing values based on peak resonance emissions(response levels or high pitched echo-sounds amplified via human body). In clinical practice, a counter or neutralizing resonance pattern calculated by the spectrometer can correct a phase-shifted resonance pattern(response levels or low pitched echo-sounds) of a diseased tissue and organ. By administering the counter resonance pattern into the site of pain and trigger point, it is possible to readjust the phase-shifted resonance pattern and then to alleviate pain through regulation of the neurotransmitter function of the nervous system. For assessing clinical effectiveness of pain relief with MRA $I_{TM}$ this study was designed to estimate pain intensity by the patient's subjective verbal rating scale(VRS such as graded to no pain, mild, moderate and severe) before application of it, to evaluate an amount of pain relief as applied the spectrometer by the patients subjective pain relief scale(visual analogue scale, VAS, 0~100%), and then to observe a continuation of pain relief following its application for managing acute and chronic pain in the 102 patients during an 8 months period beginning March, 1993. An application time of the spectrometer ranged from 15 to 30 minutes daily in each patient at or near the site of pain and trigger point when the patient wanted to be treated. The subjects consisted of 54 males and 48 females, with the age distribution between 23~40 years in 29 cases, 41~60 years in 48 cases and 61~76 years in 25 cases respectively(Table 1). The kinds of diagnosis and the main site of pain, the duration of pain before the application, and the frequency of it's application were recorded on the Table 2, 3 and 4. A distinction between acute and chronic pain was defined according to both of the pain intervals lasting within and over 3 months. The results of application of the spectrometer were noted as follows; In 51 cases of acute pain before the application, the pain intensities were rated mild in 10 cases, moderate in 15 cases and severe in 26 cases. The amounts of pain relief were noted as between 30~50% in 9 cases, 51~70% in 13 cases and 71~95% in 29 cases. The continuation of pain relief appeared between 6~24 hours in two cases, 2~5 days in 10 cases, 6~14 days in 4 cases, 15 days in one case, and completely relived of pain in 34 cases(Table 5~7). In 51 cases of chronic pain before the application, the pain intensities were rated mild in 12 cases, moderate in l8 cases and severe in 21 cases. The amounts of pain relief were noted as between 0~50% in 10 cases, 51~70% in 27 cases and 71~90% in 14 cases. The continuation of pain relief appeared to have no effect in two cases. The level of effective duration was between 6~12 hours in two cases, 2~5 days in 11 cases, 6~14 days in 14 cases, 15~60 days in 9 cases and in 13 cases the patient was completely relieved of pain(Table 5~7). There were no complications in the patients except a mild reddening and tingling sensation of skin while applying the spectrometer. Total amounts of pain relief in all of the subjects were accounted as poor and fair in 19(18.6%) cases, good in 40(39.2%) cases and excellent in 43(42.2%) cases. The clinical effectiveness of MRA $I_{TM}$ showed variable distributions from no improvements to complete relief of pain by the patient's assessment. In conclusion, we suggest that MRA $I_{TM}$ may be successful in immediate and continued pain relief but still requires several treatments for continued relief and may be gradually effective in pain relief while being applied repeatedly.

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Potent Antimutagenic Activity of Caryophyllene Oxide for Aflatoxin $B_1\;(AFB_1)$ and 2-Amino-3-Methyl-imidazo[4,5-f]Quinoline (IQ) (Aflatoxin $B_1\;(AFB_1)$과 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)에 대한 caryophyllene oxide의 항돌연변이성)

  • Lee, Jung-M.;Lee, Eun-J.;Bahn, Kyeong-N.;Kim, Jeong-O.;Ha, Yeong-L.
    • Applied Biological Chemistry
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    • v.38 no.5
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    • pp.468-472
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    • 1995
  • Substantial amount of caryophyllene oxide (CPO) is present in the essential oils of traditionally-used folk medicinal plants and herbal spices. The CPO, produced via chemical and/or enzymatic reaction of caryophyllene (CP), has largely being used as a flavoring component and exhibited a variety of biological activities. Now, we report the antimutagenic activity of CPO determined by Ames's preincubation test. S-9 fraction was prepared from the liver of rats treated with Arochor 1254. Anatoxin $B_1\;(AFB_1)$ and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) were used as mutagens. Reduction of mutagenicity of $AFB_1$ or IQ for S. typhimurium TA98 and TA100 by CPO was found to be a dose-dependant manner. CPO (500 ${\mu}g/plate$) reduced mutagenicity of AEB1 for S. typhimurium TA98 and TA100 to 89% and 71%, respectively. For IQ, similar results were observed against S. typhimurium TA98 and TA100, resulting in the inhibition percentage of 77% and 51%, respectively. CP also reduced mutagenicity of AEB1 and IQ for S. typhimurium TA98 and TA100, but the reduction rate was somewhat lowered relative to that of CPO. These results indicate that CPO could be developed as a potent antimutagenic flavoring agent.

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Studies on the Lipid Metabolism of Soybean during its Germination-(Part 2) Changes on lipoxygenase activity and fatty acid composition in soybean during germination- (대두발아(大豆發芽)중 지질대사(脂質代謝)에 관한 연구-제2보(第2報) Lipoxygenase activity 및 지방산(脂肪酸)의 변화에 관하여-)

  • Shin, Hyo-Sun
    • Applied Biological Chemistry
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    • v.17 no.4
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    • pp.247-256
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    • 1974
  • The Merit variety of soybean (Glycine max L.), harvested in 1971 was germinated in the dark at $21{\sim}25^{\circ}C$ for 10 days. The soybean sprout were divided into cotyledons and seedling axis and subjected to the determination of lipoxygenase activity and fatty acid composition of triglycerides, free fatty acids, phosholipids and crude fat fractions at two-day intervals during the germination periods. The results are summarized as follows 1) The lipoxygenase activity in cotyledons declined sharply after second day, but the activity in seedling axis inclined slightly after second day. However, the decrease of lipoxygenase activity in cotyledons coincided with decrease of linoleic and linolenic acids in cotyledons and increase of lipoxygenase activity in seedling axis coincided with increase of those acids in seedling axis. 2) The iodine value of neutral fat in cotyledons decreased continuously, but the iodine value of the neutral fat in seedling axis remained almost constant. iodine value in cotyeldons was greater than in seedling axis. 3) In the fatty acid composition of triglycerides in cotyledons, palmitic acid did not changes significantly, stearic acid increased continuously, oleic acid changed irregularly, linoleic and linolenic acids continuously decreased significantly. But in the fatty acid composition of triglycerides in seedling axis, palmitic acid remained unchanged, linoleic and linolenic acids slightly increased continuously, stearic and oleic acids changed irregularly. 4) Composition of free fatty acids in cotyledons and seedling axis changed irregularly, suggesting that all fatty acids produced by hydrolysis of triglycerides by lipase are used(or either biosynthetic Purpose or energy Production at random. 5) Fatty acids with odd-numbered carbon chain were not detected in the triglycerides and free fatty acid fractions during the germination periods, suggesting that all fatty acids are utilized as $C_2$-unit in degradation and biosynthesis. 6) The changes of fatty acids composition of Phospholipid in cotyledons and seedling axis during the germination were similar to these of triglyceride fraction.

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Preservation of Washed Fresh Ginsengs by Gamma Irradiation (방사선(放射線) 조사(照射)에 의한 수세된 수삼(水蔘)의 저장(貯藏))

  • Cho, Han-Ok;Byun, Myung-Woo;Kwon, Joong-Ho;Lee, Jae-Won
    • Applied Biological Chemistry
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    • v.29 no.3
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    • pp.288-293
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    • 1986
  • The washed fresh ginsengs packed with air, vaccum and nitrogen gas were irradiated at the levels of 1,2 and 3kGy gamma radiation and then stored at $4{\sim}5^{\circ}C$ for 90days to investigate the effects of gamma radiation on microbial inactivation, eelworm disinfestation and physicochemical changes. After a 90 day storage, $2{\sim}3kGy$ irradiated groups showed 20% of weight loss and 10% of rot while non-irradiated group 100% and 20% or more, respectively. Also the irradiated groups showed somewhat lower values of specific gravity, color density and hardness immediately after irradiation, thereafter higher value of them with storage time than those of non-irradiated group. The irradiation increased the yields of ginseng extract and crude saponins but no effects on the proximate composition and TLC and HPLC patterns of saponin. The food-borne microorganisms decreased in viable cell counts by $2{\sim}3$ log cycles with $2{\sim}3kGy$ radiation and the eelworms were completely disinfested with 1 kGy radiation.

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Studies on Wet Paddy Field Underdrainage Improvement in the Gum-Ho Area (I) (금호지구 저습답의 암거배수효과에 관한 연구(I))

  • 김조웅;김시원
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.22 no.4
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    • pp.82-95
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    • 1980
  • This paper complies the results of the studies so far made on the subsoil improvement of subsurface drainage systems for wet paddy fields (those were located in the Gum-Ho area in Kyung Buk province) which had poor permeability and a high water table. In general, a drainage problem is an excess of water on the ground surface which can effect the productivity and bearing capacity of the soil. With drain pipe systems, (According to their depths and spacing) it may be possible to correct that problem. The experimentation consisted of three test plots, two of which included drain pipe systems with varing depths and width spacing of the pipes. The third plot (C) was an ordinary plot being exempt of a drain pipe system. In detail, the depth of plot A was 80 cm, and the width spacings began at 2. Om and increased by 2. Om up to 10. 0m. The depth of plot B was 60cm and the width spacing was the same as plot A. These tests were performed to research specific details; such as crop yeild, bearing capacity of the soil, the amount of underdrainage, surface cracks, root distribution, the water table level, the consumptive water depth and the soil moisture content. The test period lasted three years, from 1977 thru 1979. The results obtained were as follows: 1. During the test period, the weather conditions for the area tested were in accordance with the annual average for that area. Furthermore the precipitation factor during the spring cultivation season, the intermediate drainage period and the harvest drainage period was of optimum conditions for controling surface cracks, because of less precipitation than evaporation. 2. The difference in the level of the ground water table in plots A and B was hardly noticable, but the difference in the test plots and the ord. plot was greatly noticable. The test plots (A, B) were 30 to 40cm lower than the ordinary plot. On the whole, the ground water table of the ord. plot always stayed at a level of 15-20cm beneath the surface of the soil, the ground water table of the test plot A showed The difference in the depth of the pipe lower than the test plot B, while the test plots showed a remarkable descending effect. 3. The soil temperature in plot A was slightly core than in plot B with a difference of 0. 47$^{\circ}$C, but plot A was 1. 6$^{\circ}$C higher than the ord. plot during the flooding period, but after drainage the temperature difference climed to 2. 0$^{\circ}$C. 4. During the 3rd test year, the values of the cracks were recorded with the values of 59cm in plot A, 42cm in plot B and 15cm in the ordinary plot. Plots A and B had increased 2.5 times the value of the first year while the ordinary plot had remained the same. 5. The root weight of the rice was measured at a value of 77.2 gr. for plot A, 73.5 gr. for plot B and 65.3 gr. for the ord. plot. Therefore, the root growths in plots A and B were much more energetic than in the ord. plot. 6. The consumptive water depth measured during the 3rd year resulted in the values of 26. 0mm per day for plot A, and 24.9 mm per day for plot B, respectively. Therefore, both plot A and plot B maintained the optimum consumptive water depths, but the ordinary plot only obtained the value of 12.3 mm per day, which clearly showed less than the optimum consumptive water depth which is 20 to 30 mm/day. 7. The soil moisture content is in direct relationship to the ground water level. During drainage, test plot A decreased in its ground water level much more rapidly than the other two plots. Therefore, plot A had a much less soil moisture content. But this decreased water level could be directly effected by the weather conditions. 8. The relationship between the bearing capacity and the soil moisture content were directly inversely proportional. It can be assumed that the occurence of soil creaks is limited by the soil moisture content. Therefore, the greater the progress of the surface creaks resulted in a greater bearing capacity. So, tast plot A with a greater amount of surface cracks than the other test plots resulted in a greater bearing capacity. But, the bearing capacity at the harvest season could be effected by the drainage during the intermediate drainage period and by the weather conditions. 9. Comparing the production of the test plots to the ord. plot; there was an increased value of 840kg for plot A, 755kg for plot B and 695kg for the ord. plot in the rough rice. Therefore, plot A had an increase of 20% over the ordinary plot. The possibility of producing double crops was investigated. The effects on barley production in the test plots showed a value of 367kg per 10 acres, which substantiated the possibility of double crops because that value showed an increased value over the average yearly yield for those uplands. 10. So as a result, it can be recommended that by including a drain pipe system with the optimum conditions of an (80cm centimeter) depth and a (l0m) spacing will have a definite positive effect on the over all production capacity and quality of wetpaddy fields.

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Determination of Safe Cropping Season in Direct- Seeding of Rice on Flooded Paddy by Using Effective Temperatures in Agroclimatic Zones (농업기후지대별 작물생육 유효기온 출현특성에 따른 벼 담수직파 안전작기 설정)

  • Shim Kyo-Moon;Lee Jeong-Taek;Yun Seong-Ho;Choi Don-Hyang
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.1 no.1
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    • pp.72-80
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    • 1999
  • The study was conducted to establish the safe cropping season for direct- seeding on flooded paddy by the analysis of meteorological data(l973~1992, 20 years) from Korea Meteorological Administration. The critical date for early seeding(CDES) at direct- seeding culture on flooded paddy was decided by the appearance date of daily mean air temperature(DMAT) of 15$^{\circ}C$. The optimum heading date(OHD) was the first day when 22$^{\circ}C$ of daily mean air temperature could be kept for 40 days of ripening period after heading, and the critical date of late heading for safe ripening(CDHR) was the last day when 19$^{\circ}C$ of daily mean air temperature could be kept for 40 days after heading. The optimum seeding date(OSD) and the critical date for late seeding(CDLS) could be decided by the accumulated temperature from OHD and CDHR to the appearance dates of necessary temperatures for early, intermediate, and intermediately late maturing varieties. This results can be used for the determination of the safe cropping season of direct-seeding on flooded paddy in each agroclimatic zone. For instance, the OSD appearance date for early maturing variety in Suwon region appeared to be May 11~20 and the CDLS appearance date was May 31~June 7.

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Effects of Elevated Atmospheric CO2 and Nitrogen Fertilization on Growth and Carbon Uptake of Yellow Poplar Seedlings (대기 이산화탄소 증가와 질소 시비가 백합나무 유묘의 생장과 탄소 흡수에 미치는 영향)

  • Chung, Mi-Sook;Han, Sim-Hee;Kim, Du-Hyun;Lee, Jae-Cheon;Kim, Pan-Gi
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.3
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    • pp.108-118
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    • 2012
  • To investigate the responses of yellow poplar (Liriodendron tulipifera L.) seedlings to the interactive effects of the elevated atmospheric $CO_2$ level and nitrogen addition, we measured biomass, photosynthetic pigments, photosynthesis, and the contents of nitrogen (N) and carbon (C) from the seedlings after 16 weeks of the treatments. Yellow poplar seedlings were grown under the ambient ($400{\mu}mol\;mol^{-1}$) and the elevated (560 and $720{\mu}mol\;mol^{-1}$) CO2 concentratoins with three different N addition levels (1.2, 2.4, and $3.6g\;kg^{-1}$) in the Open Top Chambers (OTC). The dry weight of the seedlings enhanced with the increased N levels under the elevated $CO_2$ concentrations and the increment of the dry weight differed among the different N levels. Photosynthetic pigment content of the yellow poplar leaves also increased with the increase of the $CO_2$ concentration levels. The effects of the N levels on the photosynthetic pigment content, however, were significantly different among the $CO_2$ levels. Photosynthetic rates were affected by the levels of $CO_2$ and N concentrations. Stomatal conductance and transpiration rates increased with increasing $CO_2$ concentration. The carboxylation efficiency of the seedlings without N addition increased under the higher $CO_2$ concentrations whereas that with N addition decreased under the elevated $CO_2$ concentrations. Nitrogen and carbon uptake in leaf, stem, and root increased with the elevated $CO_2$ concentration level and N addition. In conclusion, under the elevated $CO_2$ concentrations, physiological characteristics and carbon uptake of the yellow poplar seedling were improved and increased with N addition.