• Title/Summary/Keyword: kynuramine

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Inhibitory Effects of Coptisine on Monoamine Oxidase Activity

  • Lee, Myung-Koo;Lee, Kyong-Soon;Kim, Hack-Seang;Hong, Seung-Soo;Ro, Jai-Seup
    • Natural Product Sciences
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    • v.6 no.2
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    • pp.70-72
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    • 2000
  • The effects of coptisine on monoamine oxidase (EC 1.4.3.4; MAO) activity in mouse brain were investigated. Coptisine showed an inhibitory effect on MAO activity with a concentration-dependent manner. Coptisine exhibited 51.0% inhibition of MAO activity at $9\;{\mu}M$. The $IC_{50}$ value of coptisine was $8.7\;{\mu}M$. Coptisine inhibited MAO activity competitively with kynuramine as a substrate. The $K_i$ value of coptisine was $4.1\;{\mu}M$. These results indicate that coptisine functions to regulate the catecholamine content at biologically active sites.

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Effects of Herbal Medicines on Monoamine Oxidase Activity (수종의 천연물이 Monoamine Oxidase 활성에 미치는 영향 (제3보) : 황련, 계피, 지실의 활성 저해작용)

  • Lee, Sang Seon;Kim, Young Ho;Lee, Myung Koo
    • Korean Journal of Clinical Pharmacy
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    • v.8 no.2
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    • pp.139-142
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    • 1998
  • The effects of MeOH extracts from 28 herbal medicines on monoamine oxidase (MAO) activity were investigated. MAO was purified from mouse brain and its activity was determined by fluoro-photometry using kynuramine as a substrate. Three MeOH extracts, Coptis japonica, Cinnamomum cassia and Poncirus trifoliate from the herbal medicines showed a strong inhibitory effect with less than $100\;{\mu}g/ml$ in their inhibitory amounts of $50\%$ ($IC_{50}$ values) on MAO activity. Four MeOH extracts including Evodia officinalis exhibited a mild inhibition of MAO activity with $100-200\;{\mu}g/ml$ in their $IC_{50}$ values.

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Inhibition of Monoamine Oxidase by Evodiamine (Evodiamine의 Monoamine Oxidase 활성 저해작용)

  • Lee, Sang-Seon;Hwang, Bang-Yeon;Ro, Jai-Seup;Lee, Myung-Koo
    • Korean Journal of Pharmacognosy
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    • v.37 no.4 s.147
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    • pp.320-323
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    • 2006
  • The effects of evodiamine on monoamine oxidase (MAO) activity were investigated. MAO was purified from mouse brain and the $K_m\;and\;V_{max}$ values of MAO were $78.5{\pm}5.28{\mu}M$ and $0.68{\pm}0.07$ nmol/min/mg protein, respectively (n=4). Evodiamine at $30-120{\mu}M$ showed an inhibitory effect on MAO activity using a substrate kynuramine with an $IC_{50}$ value of $104.2{\mu}M$ (n=4). Evodiamine also exhibited a non-competitive inhibition on MAO. The $K_i$ value for evodiamine was $72.5{\pm}10.8{\mu}M$ (n=4). These results suggest that evodiamine partially contributes to the regulation of monoamine content.

Effects of Herbal Medicines on Monoamine Oxiclase Activity (II) (수종의 천연물이 모노아민 옥시다제 활성에 미치는 영향 (제2보))

  • Kim, Young-Ho;Lee, Sang-Seon;Bae, Ki-Hwan;Kim, Hack-Seang;Lee, Myung-Koo
    • YAKHAK HOEJI
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    • v.42 no.6
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    • pp.634-638
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    • 1998
  • The effects of MeOH extracts from 88 herbal medicines on monoamine oxidase (MAO) acitivity were investigated. MAO was purified from mouse brain and its activity was determined by fluorospectrophotometer using kynuramine as a substrate. The $K_m\;and\;V_{max}$ values (n=4) of MAO were $78.2{\pm}4.0\;{\mu}M$ and $0.65{\pm}0.05$ nmol/min/mg protein, respectively. Four MeOH extracts from Melilotus sauvelolens, Eupatorium lindleyanum Bupleurum longiradiatum and Sorbaria sirbiforia showed a strong inhibitory effect with less than $100{\mu}g/ml$ in their $IC_{50}$ values on MAO activity. Six MeOH extracts including Agastache rugosa showed a mild inhibitory effect with 100~200${\mu}$g/ml in their $IC_{50}$ values. Twenty-two MeOH extracts including Melandryum seoulense exhibited a week inhibition of MAO activity with 200~300${\mu}$g/ml in their $IC_{50}$ values.

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Effects of Herbal Medicines on Monoamine Oxidase Activity (I) (수종의 생약추출물이 Monoamine Oxidase 활성에 미치는 영향 (제1보))

  • Lee, Sang-Seon;Kim, Young-Ho;Bae, Ki-Hwan;Kim, Hack-Seang;Lee, Myung-Koo
    • Korean Journal of Pharmacognosy
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    • v.29 no.4
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    • pp.271-276
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    • 1998
  • The inhibitory effects of MeOH extracts of 100 medicinal herbs on monoamine oxidase (MAO) activity were investigated. MAO was purified from mouse brain and its activity was determined by fluorospectrophotomer using kynuramine as a substrate. Nine kinds of MeOH extracts of herbs including Artemisia iwayomogi showed a mild inhibitory effect with ${100}-{200}\;{\mu}/ml$ in their $IC_{50}$ values on MAO activity. Seventeen MeOH extracts including Juglans mandshurica exhibited a weak inhibition of MAO activity with ${200}-{300}\;{\mu}/ml$ in their $IC_{50}$ values.

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