• The Journal of Korean Academy of Prosthodontics
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• v.39 no.6
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• pp.659-681
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• 2001

Platelet-rich plasma(PRP) has been known to increase the rate and degree of bone formation by virtue of growth factors in concentrated platelets. Although its great healing effect on bone defect or pre-implantation site preparation in conjunction with bone substitute has been reported, the effect associated with implant is unknown. The purpose of this study was to investigate the effect of PRP on rapid osseointegration of endosseous dental implants in the rabbit tibiae. Twenty two adult female New Zealand white rabbits, weighing approximately 2.7-3.3kg, were used for this study. Twelve of the 22 animals were used for histomorphometric analysis and ten of the 22 were for removal torque test. Each animal received two implants in each tibia (two treated with PRP and two as control) and was given fluorochrome intramuscularly. For histomorphometric analysis, rabbits were divided into four groups according to the healing period. At 1 week, 2 weeks, 4 weeks and 8 weeks postoperatively, each three animals were sacrificed serially and the amount and rate of bone formation around dental implant were examined on the undecalcified sections under fluorescent microscope, polarized microscope and light microscope connected to a personal computer equipped with image analysis system. For removal torque test, rabbits were divided into two groups and removal torque tests were performed at 4 weeks, 10 weeks after implant placement. In total, 88 screw shaped, commercially pure titanium implants (Neoplant, Neobiotech, Seoul, Korea) were used in this study. Labeling pattern reflected differences of two groups in bone formation rate at each period. Histomorphometrically, PRP group showed significantly higher bone volume within threads compared to control group at 2 weeks ($70.30{\pm}4.96%$ vs. $50.68{\pm}6.33%$; P < .01) and 4 weeks ($82.59{\pm}5.94%$ vs. $72.94{\pm}4.57%$; P < .05 ). PRP group at 1, 2 and 4 weeks revealed similar degree of bone volume formation comparable to control group at 2, 4 and 8 weeks, respectively. On the other hand, while PRP group showed higher bone-implant contact ($47.37{\pm}8.09%$) than control group ($33.16{\pm}13.47%$) at 2 weeks, there were no significant differences between PRP group and control group for any experimental period. Removal torque values also showed no significant differences between PRP group and control group at any experimental period (P > .05). These findings imply that PRP could induce rapid, more bone formation around implant during early healing period and get faster secondary stability for reducing healing period, though it has not induced bone maturation enough to resist functional loading.

• The Journal of Korean Physical Therapy
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• v.22 no.3
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• pp.99-105
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• 2010

Purpose: The purpose of this study was to evaluate the development of pain behavior and the expression of CCL2/CCR2 and CX3CL1/CX3CR1 at above and below the level of hemisection of the spinal cord in a rat model. Methods: Spinal cords of adult female Sprague-Dawley rats (n= 16, 200~250 g, 6~8 weeks old) were hemisected at T13 on the right side to develop the spinal hemisection injury model. We compared behavioral responses of the hemisection and of a sham surgery group. Behavioral tests for motor function (by the BBB locomotor scale), and for pain response for mechanical and cold allodynia were assessed postoperatively (PO) for 21 days. Expression of mRNA for chemokines and their receptors (CCL2/CCR2 and CX3CL1/CX3CR1) below and above the level of the spinal cord dissection were examined by RT-PCR. Results: We observed gradual motor improvement and the development of mechanical and cold allodynia on the ipsilateral hindpaw after spinal hemisection injury. We also found upregulation of mRNA expression of CCL2/CCR2 both above and below the level of spinal cord dissection but CX3CL1/CX3CR1 mRNA expression. Conclusion: Upregulation of CCL2/CCR2 is associated with neuropathic pain after spinal hemisection injury. CCL2/CCR2 may play an important role in the development of neuropathic pain after SCI as well as of peripheral neuropathic pain. These findings may improve understanding of the pathophysiological mechanism of neuropathic pain after SCI.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.249-255
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• 1995

These experiments were conducted to investigate the life history and the seasonal fluctuation of population density of tea red spider mite, Tetranychus kanzawai Kishida and to observe its natural enemies in the southern area. Under field-cage conditions, tea red spider mite occurred 11~12 generations. The average periods from egg to adult emergence were 8.7~13.1 days in summer and 12.6~24.9 days in spring and autumn. The average longevities of adults were 15.5~21.9 days in summer and 25.1~31.6 days in spring and autumn. The average number of eggs laid per female were 45.1 in summer and 52.6 in spring and autumn. Tea red spider mite population showed two peaks during April-June, and appeared third or forth peak during October-November. The density of all the stages was the lowest in July through August. Tea red spider mites stayed at lower parts of tea plants in March but they moved to upper parts in April though June, and they again moved to lower parts after autumn. Tea red spider mite overwintered as all stages. Adults were the dominant overwintering stage during this stage. The surveyed natural enemies of the tea red spider mite were 7 species including Amblyseius longispinosus (Evans), A. eharai Amitei et Swirski, Anystis baccarun (L), Agistemus fleschneri Summers, Orius sauteri Poppius, Oligota yasumatsui Kistner and Scolothrips sp.

• Korean journal of applied entomology
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• v.32 no.3
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• pp.354-371
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• 1993

Behavior of adult females of the smoky brown cockroach was observed for 10-15 days continuously by using computer and the automatic sensoring system. Under the light condition of l2L~ 12D, individual variations were generally higher and the periodicity appeared less in terms of the presence time at the micro-habitats and the locomotory activity. The smoky brown cockroach appeared 20.42, 11.50, 6.31 and 2.66 % in a day in averages respectively at the shelter and the places for other individuals, feeding and drinking. It stayed 20.29 % in a day at the shelter when food, water, and other individuals were not supplied. Visiting rates were higher at the feeding and dnnking places than at. the ot.her micro-habitats. The degree of t.he locomotory activity was relatively lower when food, water, and other individuals were not supplied t.han when they were supplied. The Pnncipal Component Analysis (PCA) on the presence at the micro-habitats showed that. t.he presence pattern for each mdividual appeared differently (Q mode) while the differem time zones were grouped to the photophase and scotophase (R mode). When food, water, and other individuals were supplied the degree of grouping was higher at the shelter than at the places for feeding and drinking. When the act.ivityand the presence time at the different micro-habitats were jointly analyzed by PCA, the achvity of the female smoky brown cockroach appeared in relation with the presence time ( %) at the places for feeding and other individuals.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.381-389
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• 2014

We investigated the host fruit, seasonal occurrence, and oviposition habits of the eurytomid wasp, Eurytoma maslovskii that cause Japanese apricot fruit drops. This eurytomid wasp was found to occur in Gyeonggi-do, Chungcheongbuk-do, Chungcheongnam-do, Jeollanam-do, and Jeollabuk-do in Korea, and it attacked seeds of Japanese apricot, apricot, and peach. Overwintered larvae were observed until mid April, inside the stone of Japanese apricots. Pupation took place between late March and late April. More than 90% of the adult wasps emerged between late April and early May. The female wasps laid eggs just under the seed coat before endocarp hardening. The length of the wasp ovipositor was at least 5 mm, and hence, the diameters of fruits for oviposition would not exceed 2 cm. We observed up to five eggs per fruit, and the egg stage did not last for more than two days. Newly hatched larvae moved to the embryo, which is the first part that forms in a seed, and feeds on this. Larval competition occurs during this time; in this study, only one larva survived and matured. Thus, from the larva cycle of the wasp, most of the damaged fruits would have fallen by early June, before the harvest period.

• Journal of Korean society of Dental Hygiene
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• v.12 no.3
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• pp.621-629
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• 2012

Objectives : The purpose of this study was to examine the influential factors for the oral-health practice of adult workers and the relationship of their health-promotion life style to the factors of oral-health practice in an attempt to determine the impact of health-promotion life style. Methods : The subjects in this study were 160 workers who worked in Seoul, North-Jeolla and South-Jeolla Province, and were at the age of 20 and up. The survey was conducted from February 1 to July 20, 2011. Results : As for the relationship of the general characteristics of the workers to the factors of their health-promotion practice, gender made statistically significant differences to toothbrushing female (3.24), and there were statistically significant differences in education/concern according to more than college(2.72) scored higher academic credential(p<0.05). The group of the workers whose health-promotion life style was better in terms of all the toothbrushing(3.35), use of oral hygiene supplies(2.19), regular dental-clinic visit(2.70), dietary control(2.84) and education/concern(3.20) scored higher, and there were statistically significant differences in the factors of education/concern(p<0.05). Their health-promotion life style had an impact on regular dental-clinic visit among the factors of oral-health practice(p<0.05), and there was a positive correlation between health-promotion life style and tooth brushing, dietary control(p<0.05), regular dental-clinic visit, education and concern(p<0.01) the factors of oral-health practice. Conclusions : Workers should be stimulated to get into sound life habits to change their behavior, and they should be urged to improve their preventive health care, to get a dental checkup and ultimately to promote their health as well. Companies should take measures to accelerate the oral health promotion of workers and provide more dental checkup programs for them to be more concerned about their oral health, to promote their oral health and to maintain the best oral health.

• Journal of Korean Biological Nursing Science
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• v.1 no.1
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• pp.71-85
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• 1999

The purpose of this study was to determine the effect of periodic low-intensity exercise during hindlimb suspension on the mass, relative weight, myofibrillar protein content in soleus, plantaris and gastrocnemius muscles. To examine the effectiveness of periodic low-intensity exercise on mass, and myofibrillar protein content of hindlimb muscles, adult female Wistar rats were suspended(HS) and half of these rats walked on a treadmill for 45min/day(15 min every 4h) at 5m/min and a $15^{\circ}$ grade(HS-EX). Soleus wet weight was 33.51% significantly smaller(p<0.005) and relative soleus weight of hindlimb suspended rats was 31.96% smaller(p<0.005) compared with those of control rats following seven days of hindlimb suspension. Plantaris wet weight was 7.5% smaller(p<0.01) and relative plantaris weight was 11.83% smaller(p<0.05) compared with those of control rats following seven days of hindlimb suspension. Gastrocnemius wet weight was 11.31% significantly smaller(p<0.005) and relative gastrocnemius weight was 17.13% significantly smaller(p<0.005) compared with those of control rats following seven days of hindlimb suspension. Soleus wet weight while increased by relative soleus weight increased by 25.13%, 27.59% each through periodic low intensity exercise during hindlimb suspension(p<0.05, p<0.05). Plantaris wet weight and relative plantaris weight increased by 1.04%, 10.98%(p<0.05) each, and gastrocnemius wet weight and relative gastrocnemius weight increased by 1.98%, 12.02%(p<0.05) each through periodic low intensity exercise during hindlimb suspension. Wet weight of soleus, plantaris and gastrocnemius in HS-EX rats did not recover to control level. Myofibrillar protein content of soleus, plantaris and gastrocnemius was 48.24%, 40.85% and 37.33% significantly smaller(p<0.005) respectively compared with those of control rats following seven days of hindlimb suspension. Myofibrillar protein content of soleus, plantaris and gastrocnemius increased by 40.68%, 25.07% and 17.93%(p<0.005) each through periodic low intensity exercise during hindlimb suspension. Myofibrillar protein content of soleus, plantaris, and gastrocnemius in HS-EX rats did not recover to control level. The results suggest that periodic low intensity exercise can attenuate hindlimb muscle atrophy induced by hindlimb suspension.

• Korean Journal of Clinical Pharmacy
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• v.27 no.2
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• pp.92-98
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• 2017

Background: Nebulized colistimethate is increasingly used, because there are problems such as renal dysfunction and low distribution within the lungs when colistimethate is administered intravenously. This study was designed to compare and analyze the changes in renal function by of nebulized colistimethate treatment for its safe administration. Methods: This study retrospectively reviewed the electronic medical records of adult patients above 19 years old, receiving only the nebulized colistimethate at least 4 days in Yonsei university health system from Nov 2014 to Aug 2015. Acute kidney injury (AKI) was determined by using the RIFLE criteria (Risk, Injury, Failure, Loss and End-stage renal disease) according to serum creatinine (SCr) levels before and after use of nebulized colistimethate. Results: 48 patients were included our study and their SCr increased significantly after nebulized colistimethate treatment ($SCr_0$ vs. $SCr_1$; $0.85{\pm}0.80$ vs. $1.00{\pm}0.82mg/dL$, n=48, p<0.001), but the changes were in normal range according to the standards at Yonsei university health $system^a$. Among 48 patients, 38 patients were in the non-AKI group (79.2%), and 10 patients developed AKI (20.8%). Within the AKI group, 2 patients were in the Injury group (20%) and the other 8 in the Risk group (80%). Conclusion: There was no significant difference in age, dosage and duration of treatment between AKI group and non-AKI group (p>0.05). The study has a significance in that it reviewed the safety of nebulized colistimethate only treatment to national patients, analyzing its nephrotoxicity. It has confirmed that nebulized colistimethate is a safer method than intravenous injection, and requires to establish a guideline for the use of nebulized colistimethate in further studies with broader patient groups. $^a$ : SCr Male 0.68-1.19 mg/dL, Female 0.49-0.91 mg/dL.

• Journal of Pharmaceutical Investigation
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• v.8 no.3
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• pp.16-23
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• 1978

This study was carried out for the purpose of observing the effect of Korean Acanthopanax Radicis Cortex on renal hypertension and to clarify the mechanism of this effect, making use of its ethanol extract. Adult male or female rats, weighing 180-250g, were divided into 3 groups; the first for normotensive control, the second for hypertensive control and the third for hypertensive Acantopanax-treatment. Rats in the normotensive and hypertensive control group were administered 0.9% saline subcutaneously only, whereas those in the Acanthopanax-treated hypertensive group were administered 50mg/kg Acanthopanax ethanol extract subcutanously once a day. Changes of original blood pressure, and responses of blood pressure to various 4gents(norepinephrine, angiotensin, acetylcholine, serotonin and histamine) were recorded for each group on the initial, 18th, 32nd and 46th days of the experiment. The results obtained in this experiment are as follows: 1) The initial blood pressure was $102.6{\pm}7.6mmHg$ on the average. The blood pressures of the normotensive control group were not observed to alter significantly at any period in the course of the experiment. 2) The mean blood pressures in the hypertensive control group were recorded at $120.3{\pm}10.4mmHg$ on the 18th day, at $134.5{\pm}9.2$ on the 32nd day and at $138.8{\pm}8.3$ on the 46th day, thus revealing significant elevation in comparison with the corresponding normotensive control group blood pressures. On the other hand, the mean blood pressures in Acanthopanax-treated hypertensive group on the 18th, 32nd and 46th days were $118.3{\pm}9.7,\;129.9{\pm}8.3\;and\;120.2{\pm}8.3mmHg$ respectively. The blood pressures of the hypertensive-Acanthopanax group recorded. on the 46th day revealed a significant difference as compared with those of the corresponding hypertensive control group. 3) On the 46th day of this experiment, the responses of blood pressure to acetylcholine in the hypertensive-Acanthopanax group were suppressed significantly as compared with those of the hypertensive control group, and in the latter group, angiotensin was decreased markedly as compared with the corresponding normotensive control group. In contrast, pressor action of norepinephrine and depressor action of serotonin and histamine did not differ significantly among the three groups. These results suggest that Acanthopanax ethanol extract suppresses the induction of renal hypertension by means of a cholinergic action such as that caused by acetylcholine.

• The Korean Journal of Community Living Science
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• v.17 no.1
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• pp.141-154
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• 2006

The purpose of this study is to provide the information of the aged olds for which basic data are almost no available. The subjects were divided on the base of age 75. The elderly under 75 were named 'the young olds' and over 75 were 'the aged olds'. The aim of this research is to promote health and to improve nutrition, and the survey was made for health promotion behaviors, habits against health risk, dietary management status and diet intake. And it was conducted by 24 hr-recall method and analyzed by nutrients intake. 242 subjects were collected in 12 cities or counties in Gyeonggi Province and 20 elderly people (10 male and 10 female) were selected out of 1 village in each district. Questionnaire about health behavior and dietary management was carried out by interview method through regional home extension workers. Statistical analyses were made by SAS (version 8.1) and Chi-square tests and General Linear Models were used. Out of the subjects the aged olds over 75 was 31.9%, elementary school educated (93.5%), with spouse (40.3%), with adult children(28.6%), monthly living cost of 500-1,000 thousand won(40.3%). Mean age was 78.82 years compared with 69.75 years of the young olds. 46.8% of the aged olds used monthly pocket money over 1000 won and it was lower than 63.3% of the young olds. Only half of the aged olds had regular exercise of walking (77.8%) or with athletic equipment (17.8%). However, the young olds did more frequent walking (82.1 %) and less exercise with athletic equipment (4.8 %), which was significantly different. Kinds of disease were different with the young or the aged olds, as more proportion of cardiovascular disease(37.9%) for the young olds and joint lumbago neuralgia(41.6%) for the aged olds. Dietary management was good (3 meals per day: 93.4%, fixed mealtime: 72.4%, and regular amount: 79.9%). But there was significant difference in side dish varieties and kinds of snacks; for the aged olds only 8% had over 5 sorts (compared with 18.8% of the young olds) and the kinds of snacks were cookie, candy, juice, carbonated beverage for the aged olds (compared with noodle, milk, soybean-milk for young olds). The ratio of nutrients intake (energy, riboflavin and niacin) with RDA was significantly higher for the aged olds than that of the young olds. The surveyed subjects had no difficulties in Activities of Daily Living (ADL), but some of the aged olds had difficulties in Instrumental Activities of Daily Living (IADL) like housekeeping, using transportation, going shopping and making phone calls. These results suggest that low quality of life is linked with low economic status of the rural elderly and congregate meal at village hall would be required because of the lack of side dishes variety for the aged olds. And nutrition education program about good snacks and exercise practice would be needed for the aged olds. By operating nutrition education program the aged olds would enjoy better quality life maintaining or ameliorating IADL abilities.