• Korean Journal of Clinical Laboratory Science
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• v.44 no.3
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• pp.155-165
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• 2012

The purpose of this study was to develop a multiplex PCR for the detection of aac(6')-Ib, aph(3')-Ia, and ant(2")-Ia; the genes that encode the most clinically relevant aminoglycoside modifying enzymes (AMEs) in Gram-negative bacteria. Clinical isolates of 80 E. coli and 23 K. pneumoniae from tertiary university hospital were tested by multiplex PCR. The most prevalent AME gene was aac(6')-Ib which was found in 22.3% of the isolates. Of the total 80 E. coli isolates, 1 isolate was found to contain both aph(3')-Ia and ant(2")-Ia simultaneouly. Of the total 23 K. pneumoniae isolates, 2 isolates were found to contain both aac(6')-Ib and aph(3')-Ia, and 1 isolate was found to contain both aac(6')-Ib and ant(2")-Ia simultaneously. Annual (2005~2009) analysis of isolates that contain the AME genes were of no correlation. The sensitivity and specificity of multiplex PCR in detecting AME genes was 94.4% (34 of 36 cases) and 100%, respectively. We suggest the multiplex PCR method we developed could be highly sensitive and specific in detecting the AME genes of E. coli and K. pneumoniae. This study could be the first published investigation in which the multiplex PCR method detects aac(6')-Ib, aph(3')-Ia, and ant(2")-Ia genes.

• Textile Coloration and Finishing
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• v.15 no.6
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• pp.27-32
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• 2003

As a natural antimicrobial agent, gallnut extract was examined. The extract was separated and analyzed by LC/MS to assign the components, gallic acid, digallic acid, trigallic acid, mono-galloyl glucose, penta-galloyl glucose, etc. Minimum inhibitory concentration of gallnut extract was about 10ppm. Cotton fabrics treated with 10% owf of gallnut extracts had antimicrobial activity showing the reduction of bacteria 99.9% for both Staphylococcus aureus and Klebsiella pneumoniae.

• Korean Journal of Pharmacognosy
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• v.17 no.4
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• pp.302-307
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• 1986

Sixteen kinds of Chinese herb drugs using in digestive system have screened for antibacterial activity. It was revealed that all of the herb drug extracts showed antibacterial activity in one or more strains of microbials selected in this experiments. It is noteworthy that Aconitum trilobum showed inhibition of Klebsiella pneumoniae.

• YAKHAK HOEJI
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• v.31 no.2
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• pp.98-104
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• 1987

The compounds synthesized by authors were evaluated for their antimicrobial activities against. Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeraginosa New compounds[1, 10] possess striking potency against a wide range of microorganisms, especially the Gram negative aerobes and has spectacular activity against Enterobacteriaces, S. aureus and Ps. aeruginosa. Most of new compounds are very active in vitro against most strains of Enter- bacteriaceae and Gram positive aerobes, but moderately active against Ps. aeruginosa.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2007.10a
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• pp.592-592
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• 2007

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.251.1-251
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• 1997

No Abstract, See Full Text

• YAKHAK HOEJI
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• v.18 no.4
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• pp.249-254
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• 1974

Eighteen 2-aryl-3-(5-nitro-2-furyl) acrylamides were synthesized as antimicrobial agent, by the condensation of 2-(p-sulbstituted phenyl)-3-(5-nitro-2-furyl) acryloyl chloride with amino component, such as sulfisozxazole, sulfamethoxypyridazine and sulfadimethoxine. All compounds showed fair bacteriostatic activity against staphylococcus aureus 6538p, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae. 2-(p-Hydroxyphenyl)-and 2-(p-acetoxyphenyl) compounds also showed antimicrobial activity against Saccharomyces cerevisiae 396 and Aspergillus oryzae.

• YAKHAK HOEJI
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• v.44 no.4
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• pp.315-317
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• 2000

The in vitro activities of DA-074, a new cephalosporin against 34 various standard strains and its in vivo efficacies against 6 important strains were obtained. DA-074 showed two fold enhanced in vitro antibacterial activity against some Pseudomonas aeruginosa compared to Ceftazidime and more than 2 fold in vivo efficacy against Staphylococcus aureus Smith, Klebsiella pneumoniae 1 and Escherichia coli KC-14, compared to Cefpirome. DA-074 might be a good candidate for further evaluations.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.743-748
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• 2005

Histamine can be produced at early spoilage stage through decarboxylation of histidine in red-flesh fish by Proteus morganii, Hafnia alvei or Klebsiella pneumoniae. Allergic food poisoning is resulted from the histamine produced when the freshness of Mackerel degrades. Conversely it has been reported that there are bacteria which decompose histamine at the later stage. We isolated histamine decomposers from salted mackerel and studied the characteristics to help establish hygienic measure to prevent outbreak of salted mackerel food poisoning. All the samples were purchased through local supermarket. Histamine decomposers were isolated using restriction medium using histamine 10 species were selected. Identification of these isolates were carried out by the comparison of 16S rDNA partial sequence; as a result, we identified Pseudomonas putida strain RA2 and Halomonas marina, Uncultured Arctic sea ice bacterium clone ARKXV1/2-136, Halomonas venusta, Psychrobacter sp. HS5323, Pseudomonas putida KT2440, Rhodococcus erythropolis, Klebsiella terrigena (Raoultella terrigena), Alteromonadaceae bacterium T1, Shewanella massilia with homology of $100\%,{\;}100\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}100\%,{\;}95\%,{\;}99\%,{\;}and{\;}100\%$respectively. Turbidometry determination method and enzymic method were employed to determine the ability of histamine decomposition. Among those species Shewanella massilia showed the highest in ability of histamine decomposition. From these results we confirmed various histamine decomposer were present in salted mackerel product in the market.

• KSBB Journal
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• v.17 no.3
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• pp.255-260
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• 2002

1,3-Propanediol as a bifunctional organic compound could be used in polymerization reactions producing polyesters and polyurethanes. Byproduct containing high concentration of glycerol from fat industry was used to produce 1,3-propanediol in lower production cost as well as waste treatment. In this study, various attempts were made to increase 1,3-propanediol production under different conditions using Klebsiella pneumoniae ATCC 15380. The conversion yield and byproduct formation were influenced significantly by the fermentation pH and temperature. The optimal glycerol and nitrogen concentration for 1,3-propanediol production were found to be 25 a/L and 1%(w/v), respectively. The formation of 1,3-propanediol was optimal at pH 6.0 and temperature $35^{\circ}C$. 1,3-Propanediol production from byproduct from 2.5% glycerol was lower than that of 2.5% commercial glycerol and amounted only to 9.84 a/L from byproduct, while to 12.13 a/L from commercial glycerol.