• 제목/요약/키워드: kinetic

검색결과 5,103건 처리시간 0.03초

Kinetic Properties of Extracted Lactate Dehydrogenase and Creatine Kinase from Mouse Embryonic Stem Cell- and Neonatal-derived Cardiomyocytes

  • Zonouzi, Roseata;Ashtiani, Saeid Kazemi;Hosseinkhani, Saman;Baharvand, Hossein
    • BMB Reports
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    • 제39권4호
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    • pp.426-431
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    • 2006
  • Embryonic stem cells (ESCs), representing a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics, are capable of spontaneous differentiation into cardiomyocytes. The present study sought to define the kinetic characterization of lactate dehydrogenase (LDH) and creatine kinase (CK) of ESC- and neonatal-derived cardiomyocytes. Spontaneously differentiated cardiomyocytes from embryoid bodies (EBs) derived from mouse ESC line (Royan B1) and neonatal cardiomyocytes were dispersed in a buffer solution. Enzymes were extracted by sonication and centrifugation for kinetic evaluation of LDH and CK with spectrophotometric methods. While a comparison between the kinetic properties of the LDH and CK of both groups revealed not only different Michaelis constants and optimum temperatures for LDH but also different Michaelis constants and optimum pH for CK, the pH profile of LDH and optimum temperature of CK were similar. In defining some kinetic properties of cardiac metabolic enzymes of ESC-derived cardiomyocytes, our results are expected to further facilitate the use of ESCs as an experimental model.

열린사슬운동과 닫힌사슬운동이 큰가슴근과 위팔세갈래근의 근활성도에 미치는 영향 (The Effect of Open and Close Kinetic Chain Exercise on the Muscle Activity of Pectoralis Major and Triceps)

  • 김주오;장상훈
    • 대한물리치료과학회지
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    • 제25권1호
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    • pp.52-61
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    • 2018
  • Background: The purpose of this study was to investigate the effect of open and close kinetic chain exercise on the muscle activity of pectoralis major and triceps. Method: Twenty healthy male college students were assessed three times over two weeks. The participants were randomly assigned to OKCE(Open Kinetic Chain Exercise) and CKCE(Close Kinetic Chain Exercise). On the first day, the 7th day and the last day, The MP(mean power) and PT(peak torque) of the PM(pectoralis major) and TR(triceps) during the exercise were measured with an electromyography device and the study was compared. Result: In the present study, it was found that Statistical analysis of the measured values at the end of experimental period revealed statistically significant differences in the MP and PT values of TR and PM. Conclusion: These findings suggest that CKCE and OKCE may be an effective physical therapy intervention for strengthening muscular activity in patients with low activity in the upper limb, including normal subjects, although it is not suitable for effective exercise by selecting either CKCE or OKCE.

운동에너지탄용 복합재 이탈피의 제조에 관한 연구 (A Study on the Fabrication of the Composite Sabot for a Kinetic Energy Projectile)

  • 최재호
    • 한국군사과학기술학회지
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    • 제9권3호
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    • pp.88-94
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    • 2006
  • In order to substitute current aluminum sabot and to increase the penetration performance of the kinetic energy projectiles, the research and development program for composites sabot has been conducted. For carbon/epoxy composites sabot, unidirectional carbon fiber reinforced epoxy prepreg was chosen and thick sectioned composites preforms with the different fiber angles along the circumferential direction of sabot were prepared by compression molding under the careful processing conditions at $150^{\circ}C$ for 1hour with $70kgf/cm^2$ curing pressure. The composites sabot demonstrated a weight reduction by approximately 30% than that of current aluminum sabot. The muzzle velocity of a kinetic energy projectile with composites sabot was measured to be about 63m/s higher than that with aluminum sabot. These results imply that the penetration performance is expected to be considerably increased when the composite sabot is applied to the kinetic energy projectiles.

Energy Sustainability of an Integrative Kinetic Light Shelf Unit

  • Han, Seung-Hoon
    • KIEAE Journal
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    • 제15권3호
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    • pp.15-20
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    • 2015
  • Purpose: Suggesting a working prototype of a kinetic light shelf unit and revealing its energy efficiency by a series of building performance simulations were presented. Recently, kinetic building envelope has been an emerging technology as an innovative way to control exterior building environment, but products from many researches about the facade could not been reached to the industrialization so far. That is because its initial installation, operation and maintenance costs are still too high to use for the practical field, although buildings using kinetic envelopes could decrease their energy consumption significantly. This narrow point of view needs to be reconsidered, since buildings require great amount of energies to run their functions through the whole life and using better building components can lead to achieve much more benefits in aspects of the lifecycle cost (LCC). Method: A series of certified simulation tools like Ecotect and Green Building Studio that are normally used for researches and developments in the field of architecture were utilized. Result: Based on simulation analyses, the result of the study has showed that the proposed system definitely has adaptability to the professions and positively shows practicability as advanced integrative building envelopes with renewable energy association.

Adsorption of Pb(II) Ions from Aqueous Solution Using Activated Carbon Prepared from Areca Catechu Shell: Kinetic, Isotherm and Thermodynamic Studies

  • Muslim, A.;Aprilia, S.;Suha, T.A.;Fitri, Z.
    • 대한화학회지
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    • 제61권3호
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    • pp.89-96
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    • 2017
  • This study proposed adsorption of Pb(II) ions from aqueous solution using activated carbon prepared from areca catechu shell (ACS AC) using Timphan Method. The effects of independent variables on adsorption kinetic and isotherm have been investigated by conducting experiments in batch mode at neutral pH. The structural characterization of adsorbent was done by FT-IR and SEM analysis. The Pb(II) adsorption was correlated very well with the pseudo second-order kinetic (PSOKM) and Langmuir isotherm models (LIM). Increasing NaOH mass for activation and adsorption temperature increased weakly all the parameters of adsorption kinetic and isotherm. The Pb(II) ions adsorption capacity of the ACS AC at 27 and $45^{\circ}C$ was 50.51 and 55.25 mg/g, respectively. Thermodynamic parameters were determined, and the results confirmed the Pb(II) ions adsorption should be endothermic and spontaneous process, and both physical and chemical adsorption should be taken place.

다양한 방해석 표면에 대한 Mn과 Co 흡착 기작 (The Removal Kinetics of Mn and Co from the Contaminated Solutions by Various Calcium Carbonate Surfaces)

  • H., Yoon;Ko, K.S.;Kim, S.J.
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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    • pp.219-222
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    • 2004
  • Removal characteristics of Mn and Co was studied from the contaminated solutions via surface reaction with various calcium carbonate (calcite). Synthetic calcium carbonates which has different surface morphology as well as surface areas were prepared by a spontaneous precipitation method and used. Mn and Co removal behavior by the different solid surface demonstrate characteristic sorption behaviors depend on the type of calcite used, such as surface area or surface morphology. Calcium carbonate crystals (mostly calcite) which exhibit complicated surface morphology (c-type) shows strong sorption affinity for Mn and Co removal via sorption than on the a-type or b-type calcite crystals of less complicated surfaces. The applicability of two kinetic models, the pseudo-first-order kinetic equation and the Elovich kinetic model was examined on these sorption behavior. Elovich kinetic model was found more suitable to explain the very early stage adsorption kinetics, while the pseudo-first-order kinetic equation was successfully fitted for the adsorption kinetics after 50 hours.

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Palm Oil Mill Effluent 처리 시 Anaerobic Hybrid Reactor의 기질 제거 Kinetics 비교 (A Comparison of Substrate Removal Kinetics of Anaerobic Reactor systems treating Palm Oil Mill Effluent)

  • 오대양;신창하;김태훈;박주양
    • 상하수도학회지
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    • 제25권6호
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    • pp.971-979
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    • 2011
  • Palm Oil Mill Effluent (POME) is the mixed organic wastewater generated from palm oil industry. In this study, kinetic analysis with treating POME in an anaerobic hybrid reactor (AHR) was performed. Therefore, the AHR was monitored for its performances with respect to the changes of COD concentrations and hydraulic retention time (HRT). Batch tests were performed to find out the substrate removal kinetics by granular sludge from POME. Modified Stover Kincannon, First-order, Monod, Grau second-order kinetic models were used to analyze the performance of reactor. The results from the batch test indicate that the substrate removal kinetics of granular sludge is corresponds to follow Monod's theory. However, Grau second-order model were the most appropriate models for the continuous test in the AHR. The second order kinetic constant, saturation value constant, maximum substrate removal rate, and first-order kinetic constant were 2.60/day, 41.905 g/L-day, 39.683 g/L-day, and 1.25/day respectively. And the most appropriate model was Grau second-order kinetic model comparing the model prediction values and measured COD concentrations of effluent, whereas modified Stover-Kincannon model showed the lowest correlation.

유압식 에너지 회생시스템의 성능평가 (Performance Evaluation for Hydraulic Type Energy Regenerative System)

  • 정동수;김형의;강이석
    • 한국자동차공학회논문집
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    • 제14권2호
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    • pp.136-144
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    • 2006
  • Vehicles usually have 3 types of speed pattern like acceleration, travel, and deceleration. It requires much driving energy from engine while accelerating, preserves much kinetic energy by inertia moment at travel speed, and releases the kinetic energy to the air while decelerating by the break system. If we accumulate the kinetic energy while decelerating and reuse the energy at the accelerating stage, then it can elevate the fuel efficiency, reduce the emission and improve the motive power. This paper proposes a hydraulic type energy regenerative system which converts the kinetic energy into hydraulic energy at the stage of deceleration and reuses it at the starting and accelerating stage of vehicles. The test equipment which has the field condition of city bus was prepared to evaluate the performance for energy regeneration. The test results show that both energy regeneration efficiency and fuel efficiency are improved significantly and the emission is reduced notably.

Protein Adsorption on Ion Exchange Resin: Estimation of Equilibrium Isotherm Parameters from Batch Kinetic Data

  • Chu K.H.;Hashim M.A.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제11권1호
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    • pp.61-66
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    • 2006
  • The simple Langmuir isotherm is frequently employed to describe the equilibrium behavior of protein adsorption on a wide variety of adsorbents. The two adjustable parameters of the Langmuir isotherm - the saturation capacity, or $q_m$, and the dissociation constant, $K_d$ - are usually estimated by fitting the isotherm equation to the equilibrium data acquired from batch equilibration experiments. In this study, we have evaluated the possibility of estimating $q_m$ and $K_d$ for the adsorption of bovine serum albumin to a cation exchanger using batch kinetic data. A rate model predicated on the kinetic form of the Langmuir isotherm, with three adjustable parameters ($q_m,\;K_d$, and a rate constant), was fitted to a single kinetic profile. The value of $q_m$ determined as the result of this approach was quantitatively consistent with the $q_m$ value derived from the traditional batch equilibrium data. However, the $K_d$ value could not be retrieved from the kinetic profile, as the model fit proved insensitive to this parameter. Sensitivity analysis provided significant insight into the identifiability of the three model parameters.

가상현실을 이용한 닫힌 사슬 운동이 뇌졸중 환자의 하지 근활성도에 미치는 영향 (Effects of Virtual Reality-Based Closed Kinetic Chain Exercise on Lower Extremity Muscle Activity in Chronic Stroke Patients)

  • 양대중;박삼헌;이민기;박승규
    • 대한임상전기생리학회지
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    • 제11권1호
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    • pp.7-12
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    • 2013
  • Purpose : The purpose of this study was to identify effects of virtual reality-based closed kinetic chain (CKC) exercise on lower extremity muscle activity in chronic stroke patients Methods : Subjects were assigned randomly either to VR+CKC exercise group (n=15) or the CKC exercise group (n=15). When the study began, both groups received conventional physical therapy five times per week. The VR+CKC exercise group received virtual reality programs and the CKC exercise group received close kinetic chain exercises. Each exercise program was performed for six weeks (5 times per weeks; 20 minutes per time). Participants were measured on lower extremity muscle activity using EMG. Results : There was a significant increase of muscle activity (%RVC) in vastus lateralis and vastus medialis. Conclusion : In this study, the virtual reality-based closed kinetic chain exercise program was an effective exercise for improving lower extremity muscle activity in chronic stroke patients.