• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.828-834
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• 2010

An ethanol-producing yeast strain, CHFY0201, was isolated from soil in South Korea using an enrichment technique in a yeast peptone dextrose medium supplemented with 5% (w/v) ethanol at $30^{\circ}C$. The phenotypic and physiological characteristics, as well as molecular phylogenetic analysis based on the D1/D2 domains of the large subunit (26S) rDNA gene and the internally transcribed spacer (ITS) 1+2 regions, suggested that the CHFY0201 was a novel strain of Schizosaccharomyces pombe. During shaking flask cultivation, the highest ethanol productivity and theoretical yield of S. pombe CHFY0201 in YPD media containing 9.5% total sugars were $0.59{\pm}0.01$ g/l/h and $88.4{\pm}0.91%$, respectively. Simultaneous saccharification and fermentation for ethanol production was carried out using liquefied cassava (Manihot esculenta) powder in a 5-l lab-scale jar fermenter at $32^{\circ}C$ for 66 h with an agitation speed of 120 rpm. Under these conditions, S. pombe CHFY0201 yielded a final ethanol concentration of $72.1{\pm}0.27$ g/l and a theoretical yield of $82.7{\pm}1.52%$ at a maximum ethanol productivity of $1.16{\pm}0.07$ g/l/h. These results suggest that S. pombe CHFY0201 is a potential producer for industrial bioethanol production.

• 한국균학회지
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• 제30권2호
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• pp.131-135
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• 2002

표고버섯 균사체가 항당뇨 효과에 미치는 영향을 검토하기 위해 당뇨가 유발된 흰쥐에 표고버섯 균사체을 7일간 급여시킨 후 혈장 glucose, total cholesterol, triglyceride 및 ALT, AST 활성을 관찰하였고, 표고버섯 균사체 생장의 최적 배양 조건을 조사하였다. 균사체 생장을 위한 최적의 pH와 온도는 5와 $30^{\circ}C$로 나타났으며, 발효조배양시에는 최대 균사체 생장(8.13g/l)이 진탕배양일 때보다 6일 단축되었다. Hypoglycemic 효과에서 혈장 glucose는 당뇨 유발군에서 표고버섯 균사체 급여군이 대조군보다 23.0%가 유의하게 감소하였으며, 혈장 total cholesterol과 triglyceride는 20.3%와 27.1%로 낮게 나타났다. 또한 혈장 ALT와 AST 활성은 당뇨 대조군에 비해 표고버섯 균사체 급여군이 29.6%와 34.3% 낮은 활성을 보였다.

• 공업화학
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• 제8권4호
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• pp.660-666
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• 1997

본 연구에서는 Streptomyces peucetius subsp. caesius 돌연변이주에 의한 doxorubicin의 생산에 있어서 배양조건 및 배지의 성분을 확립하여 doxorubicind의 생산을 높이는데 목적이 있다. Doxorubicin 생산을 위한 최적 배지조성은 4% maltose, 0.5% HEPES, 0.02% $K_2HPO_4$, 0.01% $MgSO_4$로 나타났고, 가장 적합한 종균 접종량과 시기는 10% (v/v), 72시간이었다. Doxorubicin생산에 적합한 소포제를 찾기위해 여러 종류의 소포제를 배지에 첨가한 결과 가장 적합한 소포제는 KG(10% K+10% G)이었으며 최적농도는 0.01%이었다. 교반식반응기에서 배양할 경우 적합한 통기량은 1.5v/v min으로 최대 29mg/l의 doxorubicin을 생산하였고, 1.0v/v min의 경우에도 플라스크 배양보다 15% 증가된 23mg/l의 doxorubicin을 생산하였다.

• KSBB Journal
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• 제25권1호
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• pp.47-54
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• 2010

The mycelial dispersed growth of Cordyceps sinensis was optimized in submerged batch culture at initial pH of 5.0, 150 rpm, and $25^{\circ}C$. The morphological data showed much more dispersed growth of C. sinenesis at initial pH of 5.0. Also, projected area, main hyphal length and number of tips for the mycelial growth of initial pH 5.0 were higher than those of other initial pHs. The industrial medium for mycelial production of C. sinensis was determined to be molasses of 100 g and crushed brewery yeast of 10 g per liter as carbon and nitrogen sources, respectively. With these culture conditions, the maximum production of mycelia was approximately 30.0 g per liter by batch culture in 5-liter jar fermenter with no controlled pH. This result suggests that large-scale mycelia production of C. sinensis may be possible in submerged batch culture. The hot water extract of mycelia from C. sinensis was mainly composed of 83.0% carbohydrate, 11.8% protein, 1.9% lipid, and 2.4% ash and there were present glucose, mannose, galactose, and arabinose as molar ratio of 8.79 : 2.59 : 1.34 : 1.0 in the carbohydrate, respectively. In the experiment using spleen cell and macrophage, the extract showed potent mitogenic and immuno-stimulating activities and among various components, an important factor that contribute to the immunological activities was turned out to be carbohydrate moiety.

• 한국미생물·생명공학회지
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• 제31권1호
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• pp.46-50
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• 2003

젓갈에서 분리된 Lactococcus lactis SA72균주로부터 유용 박테리오신인 lacticin SA72의 생산을 최적화하기 위해 배양용 배지 및 배양조건을 검토하였다. 유산균의 대표적인 배양 배지인 BHI, MRS, Ml7배지에 대해서 검토한 결과, 이중 MRS배지가 최적 배지로 결정되었다. 이 MRS배지에 0.5% glucose를 첨가한 경우, 더 높은 활성을 얻을 수 있었다. pH의 영향을 검토하였을 때는, 초기 pH에서는 pH 7이었고, 발효조에서의 배양기간 동안 pH 6으로 조절할 때가 높은 활성을 보였다. 배양온도는 $32^{\circ}C$로 유지할 때가 활성이 우수하였다. 회분식 배양에서는 최대 3,200AU/m1을 얻을 수 있었으며, 유가식 배양으로 10g/1 glucose를 주기적으로 공급하였을 때 , 약 6,400 AU/ml로 가장 높은 활성을 얻을 수 있었다.

• 한국미생물·생명공학회지
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• 제19권3호
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• pp.265-271
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• 1991

돼지감자 분말로부터 대체에너지용 알코올을 생산하기 위하여 우수 inulin 자화 균주로 알려진 Kluyveromyces marxianus UCD(FST)55-82로 알코올 발효 최적 배양조건을 조사한 바 배양 최적 온도 30'C, 최적pH 5.44(돼직감자 자체의 pH), 교반속도 100rpm, 배지의 양 1,000ml(2.5l jar fermenter), 통기하지 않은 상태에서 알코올 생성률이 가장 높았으며 효모의 접종량은 배지량의 2.5로 충분하였다. 또한, 0.01 antifoam A concentrate(silicon polymer)와 0.1 urea의 첨가는 알코올 농도를 효과적으로 증가시키고 발효기간을 단축시켰다. 이와 같은 최적 조건에서 총당질은 배양 하루만에 89.62가 분해되어 알코올 생성 수율이 91.9, 알코올 productivity가 2.71g ethanol/l/h이었고 배양 4일 후에슨 0.7g ethanol/l/h으로 발효액의 알코올 농도는 6.8가 되었다.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.189-194
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• 2013

In a study of hydrogen-producing bacteria, strain T4384 was isolated from rice field samples in the Republic of Korea. The isolate was identified as Enterobacter sp. T4384 by phylogenetic analysis of 16S rRNA and rpoB gene sequences. Enterobacter sp. T4384 grew at a temperature range of $10-45^{\circ}C$ and at an initial pH range of 4.5-9.5. Strain T4384 produced hydrogen at 0-6% NaCl by using glucose, fructose, and mannose. In serum bottle cultures using a complete medium, Enterobacter sp. T4384 produced 1,098 ml/l $H_2$, 4.0 g/l ethanol, and 1.0 g/l acetic acid. In a pH-regulated jar fermenter culture with the biogas removed, 2,202 ml/l $H_2$, 6.2 g/l ethanol, and 1.0 g/l acetic acid were produced, and the lag-phase time was 4.8 h. Strain T4384 metabolized the hydrolysate of organic waste for the production of hydrogen and volatile fatty acid. The strain T4384 produced 947 ml/l $H_2$, 3.2 g/l ethanol, and 0.2 g/l acetic acid from 6% (w/v) food waste hydrolysate; 738 ml/l $H_2$, 4.2 g/l ethanol, and 0.8 g/l acetic acid from Miscanthus sinensis hydrolysate; and 805 ml/l $H_2$, 5.0 g/l ethanol, and 0.7 g/l acetic acid from Sorghum bicolor hydrolysate.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1233-1241
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• 2017

The ginsenoside Rh2 has strong anti-cancer, anti-inflammatory, and anti-diabetic effects. However, the application of ginsenoside Rh2 is restricted because of the small amounts found in Korean white and red ginsengs. To enhance the production of ginsenoside Rh2-MIX (comprising 20(S)-Rh2, 20(R)-Rh2, Rk2, and Rh3 as a 10-g unit) with high specificity, yield, and purity, a new combination of enzymatic conversion using the commercial enzyme Viscozyme L followed by acid treatment was developed. Viscozyme L treatment at pH 5.0 and $50^{\circ}C$ was used initially to transform the major ginsenosides Rb1, Rb2, Rc, and Rd into ginsenoside F2, followed by acid-heat treatment using citric acid 2% (w/v) at pH 2.0 and $121^{\circ}C$ for 15 min. Scale-up production in a 10-L jar fermenter, using 60 g of the protopanaxadiol-type ginsenoside mixture from ginseng roots, produced 24 g of ginsenoside Rh2-MIX. Using 2 g of Rh2-MIX, 131 mg of 20(S)-Rh2, 58 mg of 20(R)-Rh2, 47 mg of Rk2, and 26 mg of Rh3 were obtained at over 98% chromatographic purity. Then, the anti-cancer effect of the four purified ginsenosides was investigated on B16F10, MDA-MB-231, and HuH-7 cell lines. As a result, these four rare ginsenosides markedly inhibited the growth of the cancer cell lines. These results suggested that rare ginsenoside Rh2-MIX could be exploited to prepare an anti-cancer supplement in the functional food and pharmaceutical industries.

• KSBB Journal
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• 제15권5호
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• pp.507-513
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• 2000

고지혈증 치료제인 lovastatin을 Aspergillus terreus로부터 생산하기 위해 발효조 배양설험에서 교반속도와 pH에 대한 영향올 조사하였다. 최적 교반속도는 400 rpm이었고 pH는 5.8로 유지하였을 때 lovastatin 생산이 최대였으며, 교반속도 보다 pH가 lovastatin 생산에 더 많은 영향을 미치는 것으로 나타났다 L-tryptop뼈n과 L-histi빼le의 첨가시기에 따른 lovastatin 생산량을 실험한 결과, 둘다 발효초기부터 첨가하여 배양하 는 것이 효과적이었다 L-tryptophan을 발효초기에 첨가한 최 적배지와 최적 환경조건인 교반속도 400 rpm, pH 5.8에서 회 분식 배양을 수행한 결과 기본배지를 이용하여 실험한 배양 결과보다 약 10배 정도 많은 836 mg/L이었고, 생산성은 3.5 mg/L.hr였다

• KSBB Journal
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• 제31권1호
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• pp.73-78
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• 2016

The aim of this study attempted to verify the possibility of bioethanol production using wasted medium density fiberboard (wMDF). In order to produce bioethanol from wood cellulosic materials must be carried out the process of pretreatment, saccharification, fermentation and distillation. First, the wMDF was pretreated using sodium chlorite and pretreated wMDF was prepared to 8% slurry and then slurry was saccharified with the commercial enzyme (Cellic CTec3). The fermentable sugar and pH of saccharified substrate were about 5.5% glucose and 4.4, respectively. Herein we compared the results of ethanol yield according to the nutrients added or without addition to increase ethanol yield. Ethanol fermentation was finished in about 24 hours, but it was delayed in experimental group without nutrients. Ethanol content and fermentation ratio of the final fermented mash prepared by utilizing jar fermenter was 25.40 g/L and 86.64%, respectively. At this time, the maximum ethanol productivity was confirmed as 1.78 g/Lh (ethanol content 21.38 g/L, 12 h), and the overall ethanol productivity was 1.05 g/Lh (ethanol content 25.27 g/L, 24 h). Using fermented liquid we could produced bioethanol 95.37% by continuous distillator packed with copper element in laboratory scale. These results show that wMDF has a potential valuable for bioethanol production.