• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.327-334
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• 2014

Light intensity is one of the most important requirements for plant growth, affecting growth, development, survival, and crop productivity. Sunlight is the main energy source on Earth which is energy used by photosynthesis to convert light energy to chemical energy. In this study, the light use efficiency and photosynthetic characteristics of high-quality rice cultivars were evaluated after shading on ripening stage. For the study, we treated of three levels of shade (0, 50 and 70%) on rice at ripening stage and two levels of nitrogen (9 and 18 kg/10a) used three high yielding rice cultivars, such as Boramchan, Hopum, and Honong. The shade was given for the respective plots from heading up to harvesting. We were performed to determine growth survey, SPAD and chlorophyll fluorescence every 10 days interval after shading on ripening stage. At harvest stage, grain yield and yield components were determined. Results of analysis of the results representing the maximum photosynthetic efficiency of PSII, Fv/Fm, and SPAD were decreased by depending on the time at full sunlight. But shade treatments were not changed and a significant difference among cultivars did not appear. Compared with the full sunlight, shade treatments significantly delayed ripening rate and decreased rice quality of cultivated rice. Therefore, rice yield, can be reduced in proportion to the shading density is apparent, the rate of decrease was not observed difference between varieties, when protected from light 70%, and decreased to less than 50%. The adverse effects of low light intensity on the yield and yield components were not able to significantly minimize by the nitrogen level.

• Korean Journal of Agricultural Science
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• v.3 no.1
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• pp.11-16
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• 1976

This experiment was carried out to determine the most adequate type and period of nursery for the late seasonal cultivation of the new varieties of rice, Yusin and Josaengtongil. The results obtained are summarized as follows: 1. Semi-hot up-land nursery was much effective for the increase of the number of tillerings and the roots of rise seedlings compared with the semi-hot low-land nursery. The number of tillerings and roots was increased in 60 days seedlings compared with 50 or 60 days seedlings. 2. The abnormal heading has not appeared at the variety Yusin at all. The seedlings of Josaengtongil grown over 50 days at the semi-hot low-and nursery showed the abnormal heading, but the abnormal heading was not checked out at the seedlings of same variety grown for 60 days on the semi-hot upland nursery. 3. On the nursery not to cause the abnormal heading, earlier seeding was much effective for the hastening of the heading time and the hastening effect of heading time by earlier seeding was significantly greater at the variety Josaengtongil than Yusin 4. The number of ears per hill was significantly increased in the seedlings from the semi-hot up-land nursery compared with the semi-hot low-land nursery. 5. When the growing period of rice seedlings was not considered, the semi-hot up-land nursery resulted in higher yield of rice than the semi-hot low-land nursery. Considering the growing periods of rice seedlings, 40 days seedlings showed high yield at the semi-hot low-land nursery, however, at the semi-hot up-land nursery, 60 days seedlings showed higher yield of rice.

• Applied Biological Chemistry
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• v.10
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• pp.15-21
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• 1968

In order to fractionate the rice protein employing paper electrophoresis, 9 subjects of Korean rice and one Indica type, Pin Galw 50 were examined, the results were as follows. 1. Polished rice protein was separated into albumin, globulin, prolamine, and oryzenin. The amount of these fractions was determined by Kjeldahl method showing respectively 0.26%, 0.65%, 0.41%, and 5.01% in average. Albumin was extracted with deionized water, globulin with 10% NaCl, prolamine with 70% ethanol, and oryzenin with 0.05N-NaOH. 2. Albumin was extracted with deionized water and dialyzed by a cellophan tube. The supernatant was submitted to paper electrophoresis using phosphate buffer (pH 7.6, ${\mu}$ 0.18). Albumin was identified as monocomponent in all of 10 varieties under study. Globulin was extracted and dialyzed to remove the albumin. The precipitates were resolved in 10% saline solution and examined by paper electrophoresis. The globulin consists of two components in phosphate buffer(pH 7.6, ${\mu}$ 0.18) Any subject, regardless the origin, appears to contain globulin I and globulin II. Prolamine was extracted with 70% ethanol, dialayzed against deionized water, resolved with ethanol, and analyzed by Paper electrophoresis. It was proved as one component in the 70% alcoholic buffer(pH 9.0, ${\mu}$=0.0095). On the contrary, paper electrophoresis with oryzenin demonstrated two or three components in Sorensen's buffer(pH 13.0, ${\mu}$ 0.11). Yookoo 132, Dungpan 5, Kwansan, and Jaekun contain oryzenin I, oryzenin II, and oryzenin III. On the other hand, Paldal, Jinheung, Sukwang, Eunbangzu, Damakum, and Pin Galw 56 contain only oryzenin II, and oryzenin III. On the basis of these analyses a discussion of the differences between the protein fractions of 10 varieties was presented. 3. Globulin I varied from 0.22% to 0.46% (aver. 0.35%) in the amount, globulin II from 0.21 to 0.44%(aver. 0.32%), oryzenin I from 0.17% to 0.44%(aver. 0.3%), oryzenin II from 1.59% to 2.88%(aver. 2.23%), and oryzenin III from 2.02% to 3.57%(aver. 2.66%).

• Molecules and Cells
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• v.26 no.2
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• pp.146-151
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• 2008

The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

• Korean journal of applied entomology
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• v.16 no.2 s.31
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• pp.79-86
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• 1977

Four experiments were carried out under farmer's field conditions to determine economic threshold levels of major rice pests aad attempt a reduction in the number of insecticide applications. In the experiments were included check treatments, insecticide schedules representing the official recommendations to farmers, and several corrective treatments. A careful record was kept of insect pest densities and disease incidence. i) In the north at Suweon and Icheon where Chilo suppresalis. (Walk.), the striped rice borer, was active in the first generation, no significant yield differences were obtained between plots receiving several insecticide applications and those left totally unprotected. The mean yields were high (5.2-7.6t/ha). ii) First generation borer activity rising to $8.6\%$ injured tillers was below the economic threshold since no yield reduction was recorded in either japonica varieties or the high-yielding Tongil variety. iii) Evidence was obtained thst berer damage was made good by replacement of infested tillers (compensatory growth), C. suppressalis populations were always low in the second generation. iv) The experimental results obtained at Suweon and Icheon do not justify the present official recommendations of 6-7 pesticide applications. v) further south at Iri a substantial yield reduction occurred due to an early outbreak of stripe disease transmitted by Laodelphax striatellus (Fallen), the small brown planthopper; a mean of 1-2 individuals/hill was recorded immediately after transplanting. C. suppressalis probably contributed to this yield reduction. vi) Several applications against the vector failed to prevent the rapid spread of stripe to the susceptible variety in the Iri experiment: in surrounding fields the resistant Tonsil varivety was ralatively unaffected. vii) Pests of lesser importance were Nephotettix cinctieps (Uhler), Nilaparvata lugens (Stil), Sogatella furcifera (Horv..), and leaf miners.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.2
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• pp.122-126
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• 1989

The study was performed to examine varietal differences of wilting injury in terms of the root developing system and root activity. The field survey was conducted at the farmer's, and the experimental fields. The experimental investigation was done at different growth stage of rice plant in the sand culture condition. The results were summarized as follows: 1. The T/R ratio of healthy plants was greater than that of the wilted plants in Yushin. 2. Regardless of plant parts, the root activity of the wilted plants decreased in Yushin. The T/R ratio of Yushin tended to increase at the vegitative stage while the Photosynthate distribution to root decreased at the later stage. 3. The root activity and the plant growth rate were higher at vegetative growth stage, while the root activity at reproductive stage decreased. 4. The root activity of Milyang 23, which has relatively less injury of wilting, did not significantly differ between the wilted and the healthy plants. The root was thick and T/R ratio was low.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.4
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• pp.371-384
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• 1982

Highly cold tolerant varieties are requested not only at high latitute cool area but also tropical high elevated areas, and the required tolerance is different from location to location. IRRI identified 6 different types of cold tolerance required in the world for breeding purpose; a) Hokkaido type, b) Suweon type, c) Taipei 1st season type, d) Taipei 2nd season type, e) Tropical alpine type and, f) Bangladesh type. The cold tolerance requested in Korea is more eargent in Tongil group cultivars and their required tolerance is the one such as the physiological activities at low temperature are as active as in Japonica group cultivars at least during young seedling stage and reproduction stage. With conventional Japonica cultivars, such cold tolerant characters are requested as short growth duration but stable basic vegetative growth, less sensitive to high temperature and less prolonged growth duration at low temperature. The methods screening for cold tolerance were developed rapidly after the Tongil cultivar was reliesed. The facilities of screening for cold tolerance, such as, low temperature incubator, cold water tank, growth cabinet, phytotron, cold water nursery in Chuncheon, breeding nursery located in Jinbu, Unbong and Youngduk, are well established. Foreign facilities such as, cold water tank with the rapid generation advancement facilities, cold nurseries located in Banaue, Kathmandu and Kashimir may be available for the screening of some limitted breeding materials. For the reference, screening methods applied at different growth stages in Japan are introduced. The component characters of cold tolerance are not well identified, but the varietal differences in a) germinability, b) young seedling growth, c) rooting, d) tillering, e) discolation, f) nutrition uptake, g) photosynthesis rate, h) delay in heading, i) pollen sterility, and j) grain fertility at low temperature are reported to be distinguishable. Relationships among those traits are not consistent. Reported studies on the inheritance of cold tolerance are summarized. Four or more genes are controlling low temperature germinability, one or several genes are controlling seedling tolerance, and four or more genes are responsible for the pollen fertility of the rice treated with cold air or grown in the cold water nursery. But most of those data indicate that the results may come out in different way if those were tested at different temperature. Many cold tolerant parents among Japonicas, Indicas and Javanicas were identified as the results of the improvement of cold tolerance screening techniques and IRTP efforts and they are ready to be utilized. Considering a) diversification of germ plasm, b) integration of resistances to diseases and insects, c) identification of adaptability of recommending cultivars and, d) systematic control of recommending cultivars, breeding strategies for short term and long term are suggested. For short term, efforts will be concentrated mainly to the conventional cultivar group. Domestic cultivars will be used as foundation stock and ecologically different foreign introductions such as from Hokkaido, China or from Taiwan, will be used as cross parents for the adjustment of growth durations and synthsize the prototype of tolerances. While at the other side, extreme early waxy Japonicas will be crossed with the Indica parents which are identified for their resistances to the diseases and insects. Through the back corsses to waxy Japonicas, those Indica resistances will be transfered to the Japonicas and these will be utilized to the crosses for the improvement of resistances of prototype. For the long term, efforts will be payed to synthsize all the available tolerances identified any from Japonicas, Indicas and Javanicas to diversify the germ plasm. The tolerant cultivars newly synthsized, should be stable and affected minimum. to the low temperature at all the growing stages. The resistances to the diseases and insects should be integrated also. The rapid generation advancement, pollen culture and international cooperations were emphasized to maximize the breeding efficiency.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.405-412
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• 1993

This experiment was conducted to investigate the effects of water depths on seedling stand and early growth of califonia rice varieties, S201, M202, A301, Italico livorno and Korean variety, Hwaseongbyeo, and barnyardgrass (Echinochloa crus-galli) The coleoptile length of rice was longer with deep water depth while for the radicle length shorten. As water depth was increased, the percentage of seedling stand were decreased slightly in rice, while sharply increased in barnyardgrass. Plant height of rice with increasing water depth were longer, whereas that of barnyardgrass reduced significantly with weaker. Tiller number of rice and barnyardgrass were significantly reduced as water depth increased. Dry matter weight and healthy score of rice seedling at 35DAS were highest in 7.5cm water depth followed saturated moisture, 15, and 22.5cm water depth, while for barnyardgrass those were especially negatively affected by deep water depth. These results showed that the seedling stand and early growth of barnyardgrass was highly suppressed by deeper water levels compared with rice. Rice cultivars which are showes growth characteristics in deeper water levels at early growth stage were Italico livorno and S201 in Japonica / Indica.

• Korean Journal of Soil Science and Fertilizer
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• v.21 no.3
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• pp.316-338
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• 1988

To identify the physiological phenomena on the sudden wilting of japonica/indica crossed varieties, Pot experiment was carried out under the heavy N application with various levels of potassium in Japan. The results obtained are as follows. 1. Sudden wilting was occurred in both varieties used, Yushin and Milyang 23. The former showed a higher degree than the latter. 2. Sudden wilting was occurred into two types, one at early ripening stage and the other at late ripening stage. The former type was found in the field with low potassium supply and the latter was seemed to be related to varietal wilting tolerence. 3. By the investigation of concerning the effective tillering rate and the change of dry weight of each organ at the heading stage, it was inferred that the growth status from young panicle formation stage to heading stage were related to sudden wilting tolerence. 4. Manganese content at heading stage, ratio of Fe/Mn and Fe. Fe/Mn in stern at late ripening stage and $K_2$ O/N ratio of stem at harvesting stage were recognized as the specific factors in connection with sudden wilting. Mn content in the sudden wilting rice plant was already in creased remarkably at heading stage. In relation to root age and absoption characteristics of Mn, the senility of root before heading stage was inferred as the cause of increase the value of Fe/Mn or Fe. Fe/Mn. 5. The $K_2$ O/N ratio of culm at harvesting stage was lower in upper node than lower node in relation to sudden wilting. And it was well accordance with the fact that the symptoms of sudden wilting proceeded from upper leaf to lower leaf. These phenomenon was different from the usual one that the effect of potassium deficiency was more remarkable in lower node than upper node. 6. All varieties which have a condition of potassium deficiency have a high degree of nitrogen content of leaves at heading stage and the $K_2$ O/N ratio of each organ was low, Especialy, $K_2$ O/N ratio is much lower in sheath and culm than leaves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.1-40
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• 1973

There is a general tendency to increase nitrogen level in rice production to insure an increased yield. On the other hand, percentage of ripened grains is getting decreased with such an increased fertilizer level. Decreasing of the percentage is one of the important yield limiting factors. Especially the newly developed rice variety, 'Tongil' is characterized by a relatively low percentage of ripened grains as compared with the other leading varieties. Therefore, these studies were aimed to finding out of some measures for the improvement of ripening in rice. The studies had been carried out in the field and in the phytotron during the period of three years from 1970 to 1972 at the Crop Experiment Station in Suwon. The results obtained from the experiments could be summarized as follows: 1. The spikelet of Tongil was longer in length, more narrow in width, thinner in thickness, smaller in the volume of grains and lighter in grain weight than those of Jinheung. The specific gravity of grain was closely correlated with grain weight and the relationship with thickness, width and length was getting smaller in Jinheung. On the other hand, Tongil showed a different pattern from Jinheung. The relationship of the specific gravity with grain weight was the greatest and followed by that with the width, thickness and length, in order. 2. The distribution of grain weight selected by specific gravity was different from one variety to another. Most of grains of Jinheung were distributed over the specific gravity of 1.12 with its peak at 1.18, but many of grains of Tongil were distributed below 1.12 with its peak at 1.16. The brown/rough rice ratio was sharply declined below the specific gravity of 1.06 in Jinheung, but that of Tongil was not declined from the 1.20 to the 0.96. Accordingly, it seemed to be unfair to make the specific gravity criterion for ripened grains at 1.06 in the Tongil variety. 3. The increasing tendency of grain weight after flowering was different depending on varieties. Generally speaking, rice varieties originated from cold area showed a slow grain weight increase while Tongil was rapid except at lower temperature in late ripening stage. 4. In the late-tillered culms or weak culms, the number of spikelets was small and the percentage of ripened grains was low. Tongil produced more late-tillered culms and had a longer flowering duration especially at lower temperature, resulting in a lower percentage of ripened grains. 5. The leaf blade of Tongil was short, broad and errect, having light receiving status for photosynthesis was better. The photosynthetic activity of Tongil per unit leaf area was higher than that of Jinheung at higher temperature, but lower at lower temperature. 6. Tongil was highly resistant to lodging because of short culm length, and thick lower-internodes. Before flowering, Tongil had a relatively higher amount of sugars, phosphate, silicate, calcium, manganese and magnesium. 7. The number of spikelets of Tongil was much more than that of Jinheung. The negative correlation was observed between the number of spikelets and percentage of ripened grains in Jinheung, but no correlation was found in Tongil grown at higher temperature. Therefore, grain yield was increased with increased number of spikelets in Tongil. Anthesis was not occurred below 21$^{\circ}C$ in Tongil, so sterile spikelets were increased at lower temperature during flowering stage. 8. The root distribution of Jinheung was deeper than that of Tongil. The root activity of Tongil evaluated by $\alpha$-naphthylamine oxidation method, was higher than that of Jinheung at higher temperature, but lower at lower temperature. It is seemed to be related with discoloration of leaf blades. 9. Tongil had a better light receiving status for photosynthesis and a better productive structure with balance between photosynthesis and respiration, so it is seemed that tongil has more ideal plant type for getting of a higher grain yield as compared with Jinheung. 10. Solar radiation during the 10 days before to 30 days after flowering seemed enough for ripening in suwon, but the air temperature dropped down below 22$^{\circ}C$ beyond August 25. Therefore, it was believed that air temperature is one of ripening limiting factors in this case. 11. The optimum temperature for ripening in Jinheung was relatively lower than that of Tongil requriing more than $25^{\circ}C$. Air temperature below 21$^{\circ}C$ was one of limiting factors for ripening in Tongil. 12. It seemed that Jinheung has relatively high photosensitivity and moderate thermosensitivity, while Tongil has a low photosensitivity, high thermosensitivity and longer basic vegetative phase. 13. Under a condition of higher nitrogen application at late growing stage, the grain yield of Jinheung was increased with improvement of percentage of ripened grains, while grain yield of Tongil decreased due to decreasing the number of spikelets although photosynthetic activity after flowering was. increased. 14. The grain yield of Jinheung was decreased slightly in the late transplanting culture since its photosynthetic activity was relatively high at lower temperature, but that of Tonil was decreased due to its inactive photosynthetic activity at lower temperature. The highest yield of Tongil was obtained in the early transplanting culture. 15. Tongil was adapted to a higher fertilizer and dense transplanting, and the percentage of ripened grains was improved by shortening of the flowering duration with increased number of seedlings per hill. 16. The percentage of vigorous tillers was increased with a denser transplanting and increasing in number of seedlings per hill. 17. The possibility to improve percentage of ripened grains was shown with phosphate application at lower temperature. The above mentioned results are again summarized below. The Japonica type leading varieties should be flowered before August 20 to insure a satisfactory ripening of grains. Nitrogen applied should not be more than 7.5kg/10a as the basal-dressing and the remained nitrogen should be applied at the later growing stage to increase their photosynthetic activity. The morphological and physiological characteristics of Tongil, a semi-dwarf, Indica $\times$ Japonica hybrid variety, are very different from those of other leading rice varieties, requring changes in seed selection by specific gravity method, in milling and in the cultural practices. Considering the peculiar distribution of grains selected by the method and the brown/rough rice ratio, the specific gravity criterion for seed selection should be changed from the currently employed 1.06 to about 0.96 for Tongil. In milling process, it would be advisable to bear in mind the specific traits of Tongil grain appearance. Tongil is a variety with many weak tillers and under lower temperature condition flowering is delayed. Such characteristics result in inactivation of roots and leaf blades which affects substantially lowering of the percentage of ripened grains due to increased unfertilized spikelets. In addition, Tongil is adapted well to higher nitrogen application. Therefore, it would be recommended to transplant Tongil variety earlier in season under the condition of higer nitrogen, phosphate and silicate. A dense planting-space with three vigorous seedlings per hill should be practiced in this case. In order to manifest fully the capability of Tongil, several aspects such as the varietal improvement, culural practices and milling process should be more intensively considered in the future.he future.