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Geographic Variations and DNA Polymorphisms in Gizzard-shad (Konosirus punctatus) (전어 (Konosirus punctatus)의 지리적 변이와 DNA 다형성)

  • Park, Su-Young;Kim, Jong-Yeon;Yoon, Jong-Man
    • Korean Journal of Ichthyology
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    • v.18 no.4
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    • pp.300-310
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    • 2006
  • Genomic DNA isolated from three geographical gizzard-shad (Konosirus punctatus) populations in Seocheon (SC), Busan (BS) and Gochang (GC) collected in the West Sea and the southern sea, respectively, off the Korean Peninsula, were PCR-amplified repeatedly. Eight selected decamer and 20-mer primers generated a total of 713 loci in the SC population, 791 in the BS population, and 732 in the GC population, with a DNA fragment size ranging from 100 bp to 2,800 bp. We identified 50 unique loci for the SC population, 70 unique loci for the BS population and 130 for the GC population: 120 shared loci for the three populations. There were 108 specific loci (15.1%) for the SC population, 74 (9.4%) for the BS population, and 67 (9.2%) for the GC population. Eight primers also generated 48 polymorphic loci (6.7%) for the SC population, 26 (3.3%) for the BS population, and 16 (2.2%) for the GC population. The similarity matrix ranged from 0.756 to 0.936 for the SC population, from 0.800 to 0.938 for the BS population, and from 0.731 to 0.959 for the GC population. The dendrogram obtained by the eight primers indicates three genetic clusters: cluster 1 (SEOCHEON 01~SEOCHEON 10), cluster 2 (BUSAN 11~BUSAN 20 and GOCHANG 23~GOCHANG 24), and cluster 3 (GOCHANG 21, 22, 25, 26, 27, 28, 29 and 30). As stated above, some individuals of the GC population appear to belong in BS population. When seeing this result, it was thought with the fact that some individuals of 2 populations seem to come and go partially. Thus, RAPD-PCR analysis revealed a significant genetic distance between the three geographical gizzard-shad populations. Using various decamer and 20-mer primers, RAPD-PCR may be applied to identify specific/polymorphic markers that are particular to a species and geographic population, and to define genetic diversity, polymorphisms, and similarities among geographical gizzard-shad populations.

Study on Strain Measurement of Agricultural Machine Elements Using Microcomputer (Microcomputer를 이용(利用)한 농업기계요소(農業機械要素)의 Strain 측정(測定)에 관(關)한 연구(硏究))

  • Kim, Kee Dae;Kim, Tae Kyun;Kim, Soung Rai
    • Korean Journal of Agricultural Science
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    • v.8 no.1
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    • pp.90-96
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    • 1981
  • To design more efficient agricultural machinery, the accurately measuring system among many other factors is essential. A light-beam oscillographic recorder is generally used in measuring dynamic strain but it is not compatible with the extremely high speed measuring system such as 1,000 m/s, also is susceptable to damage due to vibration while using the system in field. The recorder used light sensitive paper for strip chart recording. The reading and analysis of data from the strip charts is very cumbersome, errorneous and time consuming. A microcomputer was interfaced with A/D converter, microcomputer program was developed for measuring, system calibration was done and the strain generated from a cantilever beam vibrator was measured. The results are summarized as follows. 1. Microcomputer program was developed to perform strain measuring of agricultural machine elements and could be controled freely the measuring intervals, no. of channels and no. of data. The maximum measuring speed was $62{\mu}s$. 2. Calibration the system was performed with triangle wave generated from a function generator and checked by an oscilloscope. The sampled data were processed using HP 3000 minicomputer of Chungnam National University computer center the graphical results were triangle same as input wave and so the system have been out of phase distorsion and amplitude distorsion. 3. The strain generated from a cantilever beam vibrator which has free vibration period of 0.019 second were measured by the system controlled to have l.0 ms of time interval and its computer output showing vibration curve which is well filted to theoretical value. 4. Using microcomputer on measuring the strain of agricultural machine elements could not only save analyzing time and recording papers but also get excellent adaptation to field experiment, especially in measurement requiring high speed and good precision.

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Effect of Venture Capitalists on the ChiNext IPO First-Day Return in China (중국 차이넥스트 시장의 벤처캐피탈이 IPO 첫날 수익률에 미치는 영향)

  • Kang, Kai;Ahialey, Joseph Kwaku;Kang, Ho-Jung
    • Management & Information Systems Review
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    • v.36 no.4
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    • pp.117-127
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    • 2017
  • In recent times the size of the world IPO in general has skyrocketed. Specifically, China's financial market development is becoming important as both the size of China's capital market and the number of companies going public are gradually increasing. This has led to a rapid development of venture vapital(VC) institutions in China for the past couple of decades. This study focuses on one of the three markets of China's Shenzhen Stock Exchange-the Growth Enterprise Board((GEB) hereafter, ChiNext). The ChiNext is established in October, 2009 to enable hi-tech or high growth potential technology companies that find it relatively difficult to fulfil the listing requirements of either the Shenzhen Main Board or Small and Medium Size Enterprise Board(SMEB) to go public. This study covers a three-year period(2012/01/-2015/01) and analyze first day initial return of 83 venture capital-backed companies and 53 non-venture capital-backed companies using T-test. Regression analysis is used as to examine the variables affecting IPO's first-day return. The empirical results are four-fold. First, the level of first day return of venture-backed is significantly lower than non venture capital backed support in the Chinese venture capital market. Second, the level of first-day return of listed companies supported by foreign venture capital is significantly higher than that of companies receiving domestic venture capital support. Third, the firms that have a large number of venture capital firms showed a low level of first-day return. Fourth, regression result for the IPO first-day return which is as dependent variable indicates that the venture capital support(VCAP), number of venture capital(VCNum), offering size(Lnsize) and PER all affect have negative effect on the first day initial return. Also, the venture capital type(VCType), turnover ratio and the the firm type(Tech-firms) statistically affect IPO first day return positively. Finally, by shedding more light on the IPO first-day return, this paper provides meaningful information to investors about the Chinese IPO market.

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A Study on the Relationships between the Oral Health Activities and Oral Health Conditions of the Elderly (노인의 구강보건행위와 구강건강상태와의 관련성 조사 연구)

  • Gwon, Mi-Young;Young, Jin-Young
    • Journal of dental hygiene science
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    • v.6 no.4
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    • pp.271-276
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    • 2006
  • This study set out to analyze the relationships between the senior citizens' dental health activities and dental health conditions, to provide basic data to develop a program for their better dental health, and finally to search for the ways to improve their life quality through dental health care. The subjects were 142 senior citizens(58 were male and 84 were female) who were 65 years old or older and visited a dental hospital or clinic in the Seoul metropolitan area from April 17 to April 28, 2006. With the cooperation from the dentists, the investigators examined their dental conditions and conducted one-on-one interviews to collect the information about their demographic characteristics, dental health activities, and subjective dental health conditions. The collected data were analyzed with T-test, ANOVA and Pearson's correlation coefficient using the SPSS WIN 11.5 program. The analysis results were summarized as follows: First, 58 men(40.8%) and 84 women(59.2%) consisted of the subject group, where percentage of the female participants was bigger Those who were aged from 65 to 69 made the biggest age group in the subjects, accounting for 58.5% with 83. Second, those who had three dental caries or less and then didn't treat them or treated them(F) accounted for the biggest percentage with 117(82.4%) and 72(50.7%) respectively. As for the remaining teeth, the biggest number of them(40 subjects, 28.2%) had three or less remaining. Third, it turned out the female subjects had a higher level of dental health activities than their male counterparts(P = 0.00). As for living expenses, those who were paid salary or earned income themselves carried out their dental health activities in a higher level than those who lived on the benefit from the government(p = 0.02). Fourth, the subjects' subjective dental health conditions had negative correlations with their dental caries not treated. And there were positive correlations between their dental caries not treated and dental caries lost and between their dental caries treated and remaining teeth. Fifth, those subjects who earned their living expenses themselves had the most dental caries treated at 5.4(p = 0.02), and there was statistically significant difference with the numbers. Sixth, those subjects who brushed their teeth in their own manner had 11.8 teeth lost(p = 0.05), which was more than the number of those who brushed their teeth in other methods. And there was statistically significant difference among them. The remaining teeth were found most at 17.3(p = 0.00) among those who brushed their upper and lower teeth separately. In addition, those who visited the public health center often had significantly more teeth treated(4.3) than others(p = 0.00).

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The Help of Experienced Dental Hygienists Turnover Verbal Abuse and Emotional Reaction, and the Resulting Relationship (치과위생사가 경험하는 언어폭력과 그에 따른 정서적 반응 및 이직의도와의 관계)

  • Lee, Jung-Hwa;Choi, Jung-Mi;Lee, Yeong-Ae
    • Journal of dental hygiene science
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    • v.14 no.4
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    • pp.563-570
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    • 2014
  • The purpose of this study was to investigate the degree of verbal violence against dental hygienists, their emotional reaction and the relation between their intention of job transfer and verbal violence so that it could offer the basic data for developing the way how to cope with verbal violence and for improving their performance. Two hundred fifty-seven dental hygienists working for dentists' in Busan were interviewed from May 17 to 31, 2014 to collect data, of which analysis was as follows: 1) As a result of verbal violence done by patients and their guardians, 80.5% said that they experienced crude language with 17.5% forceful and imperative sentence, and 13.2% ignorant statements about their job. They were exposed to verbal violence once or twice every 6 months. As a result of researching verbal violence of co-working senior or junior hygienists, 52.1% answered that their co-working senior or junior hygienists talked crude language and 38.1% said their co-workers happened to say crude language to them. The crude language experience was relatively high as 20.2% and once or twice a week. As a result of verbal violence done by dentists, 47.5% said that they've heard crude language and 34.6% said that they experienced forceful and imperative sentence. 2) The overall average of the intention to transfer their job was $3.06{\pm}1.03$, while the highest intention of job transfer was $3.11{\pm}0.91$ where they said I have once wanted to transfer my job. 3) As a result of seeing the relation among verbal violence, emotional reaction and the intention of job transfer, there was co-relation between verbal violence and the patients' age (p<0.01); there were also co-relation between verbal violence of patients, co-workers and dentists (p<0.01). There was also significant relation between emotional reaction on verbal violence and their intention of job transfer (p<0.001).

An Analysis on Elementary School Students' Oral Health Knowledge and Education Effect in School-Based Oral Health Clinic (학교구강보건실 운영 초등학교 학생의 구강보건지식과 교육효과분석)

  • Yang, Jin-Young
    • Journal of dental hygiene science
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    • v.14 no.2
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    • pp.248-255
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    • 2014
  • The objective of the paper is to analyze the extent to which elementary school students acquired, perceived, and practiced oral health knowledge provided by an elementary school-based oral health clinic (SBOHC). It is respectively investigated by gender and year of school. The paper will be relevant for developing specified oral health programs in elementary SBOHCs. By choosing three elementary schools that operate SBOHC in the school district of Daejeon Metropolitan City from April 11 to April 30, 2013, a self-administered questionnaire was given out to be answered directly by 350 students in the 4th, 5th, and 6th grades and their responses were collected. Of the collected responses, 326 responses were explored by using the SPSS 12.0. Those students' scores of oral health knowledge are found to be statistically meaningful for school year, not for gender. In them are included correct tooth brushing time, dental caries symptoms, gum disease symptoms, and correct tooth brushing method. Change in tooth brushing method after oral health education showed the statistically meaningful difference for gender and school year. In conclusion, every SBOHC should develop a variety of specified educational programs that depend on gender and grade in each elementary school.

Synthesis and Characterization of Magnetic Core-shell ZnFe2O4@ZnO@SiO2 Nanoparticles (Magnetic Core-shell ZnFe2O4@ZnO@SiO2 Nanoparticle의 합성과 성질에 관한 연구)

  • Yoo, Jeong-Yeol;Lee, Young-Ki;Kim, Jong-Gyu
    • Journal of the Korean Chemical Society
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    • v.59 no.5
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    • pp.397-406
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    • 2015
  • ZnO, II-VI group inorganic compound semi-conductor, has been receiving much attention due to its wide applications in various fields. Since the ZnO has 3.37 eV of a wide band gap and 60 meV of big excitation binding energy, it is well-known material for various uses such the optical property, a semi-conductor, magnetism, antibiosis, photocatalyst, etc. When applied in the field of photocatalyst, many research studies have been actively conducted regarding magnetic materials and the core-shell structure to take on the need of recycling used materials. In this paper, magnetic core-shell ZnFe2O4@SiO2 nanoparticles (NPs) have been successfully synthesized through three steps. In order to analyze the structural characteristics of the synthesized substances, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR) were used. The spinel structure of ZnFe2O4 and the wurtzite structure of ZnO were confirmed by XRD, and ZnO production rate was confirmed through the analysis of different concentrations of the precursors. The surface change of the synthesized materials was confirmed by SEM. The formation of SiO2 layer and the synthesis of ZnFe2O4@ZnO@SiO2 NPs were finally verified through the bond of Fe-O, Zn-O and Si-O-Si by FT-IR. The magnetic property of the synthesized materials was analyzed through the vibrating sample magnetometer (VSM). The increase and decrease in the magnetism were respectively confirmed by the results of the formed ZnO and SiO2 layer. The photocatalysis effect of the synthesized ZnFe2O4 @ZnO@SiO2 NPs was experimented in a black box (dark room) using methylene blue (MB) under UV irradiation.

Underlying Etiologic Factor of Recurrent Pneumothorax after Bullectomy (원발성 기흉환자에서 재수술의 원인)

  • 윤용한;이두연;김해균;홍윤주
    • Journal of Chest Surgery
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    • v.32 no.6
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    • pp.556-560
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    • 1999
  • Background: The cause of spontaneous pneumothorax is not yet but it is certain that intrathoracic air comes from ruptured bulla. Video-assisted thoracoscopic surgery(VATS) or open thoracotomy is recommended for thoracic incision in recurrent pneumothorax. However, recurrent rate after bullectomy with the VATS is very high compared to mini-thoracotomy, 3% to 20% and below 2%, respectively. Material and Method: This retrospective analysis was performed on 16 re-operated cases among 446 surgically treated pneumothorax of the 737 cases of spontaneous pneumothorax diagnosed at Yongdong Severance Hospital from Nov. 1992 to June 1997. Result: Among the 446 surgically-treated patients in 737 case of spontaneous pneumothorax, 16 patients underwent re-operation, showing a 3.5% re-operation rate. Male-to-female ratio was 15 to 1 and mean age at initial attack was 20.2 years(ranging from 15 to 50). Mean hospital stay was 6.34 days(ranging from 2 to 20 days) and mean chest tube indwelling period was 4.2 days(ranging from 1-10 days). Median follow-up was 46 months(range 10-66 months). Three different surgical methods were applied : video-assisted thoracoscopic surgery(VAST) in 281 cases, of whom 2 underwent local anesthesia; subaxillary mini-thoracotomy in 159 cases and limited lateral thoracotomy in the remaining 6 cases. Three different re-operative surgical methods were applied ; video-assisted thoracoscopic surgery (VAST) in 6 cases, subaxillary mini-thoracotomy in 9 cases, and limited lateral thoracotomy in the remaining 1 case. The underlying etiological factors of the recurrent pneumothorax after bullectomy were o erlooking type(9) and new growing type(7). Mean recurrent period from previous operation was 1 month for overlooking type and 18 months for new growing type. Conclusion: The underlying etiological factors of recurrent pneumothorax lead to re-operation were new-growing and over-looking type. We need additional treatments besides resecting blebs of prevent the recurrence rate and more gentle handling with forceps due to less damage to the pleura.

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A Study on Development of Achievement Standards and Assessment Standards of Vocational Inquiry Section for 2005 College Scholastic Ability Test - Focus on Food and Nutrition Subject in the Field of Home Economics Order - (2005 수능 직업탐구영역의 과목별 성취기준과 평가기준 개발 - 식품과 영양 과목을 중심으로 -)

  • Na Hyeon-Ju;Min Kyung-Hee;Lee Hwa-young;Pyo Jum-sun;Ha Mi-ok;Jang Myung-Hee
    • Journal of Korean Home Economics Education Association
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    • v.17 no.2
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    • pp.197-219
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    • 2005
  • This study attempted, in accordance with the National Educational Curriculum, to develop achievement assessment standards for a course within the field of home economics which has been widely adopted by Korean vocational high schools, namely, the food and nutrition subject. Focus was also placed on strengthening the management of the curriculum for this food and nutrition course, as well as on establishing proper assessment standards by developing model assessment tools which can be used to assess the subject. The results of this study can be summarized as follows : First, based on an analysis of the related literature and materials. the desired notion of the achievement and assessment standards was established, and their significance ascertained the achievement and assessment standards for the food and nutrition course were set and the type of model assessment tool which should be developed, as well as the method in which it should be applied. was established Second. by analyzing the curriculums and the contents of the textbooks used in the food and nutrition subject, the researcher was able to compile the 70 factors which could to be used to develop the achievement and assessments standards, and then classify these into 6 main categories and 32 sub-categories. Based on the characteristics of these factors and learners' academic performance levels the number of factors was expanded to 89 in order to establish the achievement standards. In turn, these achievement standards were used, in accordance with the learners' achievement and teaming activity levels, to develop three different levels of assessment standards. namely, upper, middle, and lower ones. Third. a model assessment tool was developed which could be used by individual school units as a reference in terms of achievement and assessment standards, and that could be modified to meet each school's circumstances. In order to create the model assessment tool a 100-question questionnaire was formulated that contained various types of questions, such as essay, report, theoretical and practical, portfolio, as well as multiple choice-type questions. Lastly, the researcher introduced measures to effectively use the achievement and assessment standards developed for the food and nutrition course, as well as the model assessment tool in school units.

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A Study on the Chemical Index of Alteration of Igneous Rocks (화성암의 화학적 변질지수에 관한 연구)

  • Choi, Eun-Kyeong;Kim, Sung-Wook;Kim, In-Soo;Lee, Kyu-Hwan
    • Journal of the Korean Geotechnical Society
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    • v.28 no.5
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    • pp.41-54
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    • 2012
  • The weathering process of rocks leads to the reduction of geotechnical bearing capacity. The weathering of granite is frequently used to refer to the degradation of geotechnical property in the design and construction of infra-structure. In this study, the range of values of CIA (chemical index of alteration) and the change of mineral compositions by weathering have been analysed with igneous rock, which covers 45.5% in South Korean territory. Several weathering indices were studied for various rocks found in Korea and significant relationships between different indices were delineated via statistical analysis. The applicability of CIA was found to be the most significant among all weathering indicies. The composition of illite, the secondary weathering residual, generally increases for the felsic rock, and swelling clay material is not included. The weathering of felsic rock will follow a sequential process, starting from bed rock, illite, and chlorite to kaoline. The mafic rock will show weathering process, from bed rock, smectite, and chlorite to kaoline. The intermediate rocks such as andesite and tuff will show similar weathering procedure and the composition of kaoline, chlorite, and smectite tends to increase more than that of illite when the mafic rock is dominated. This means the increase of rock material which has high CEC (cation exchange capacity) during secondary weathering process. However, the characteristics of a specific rock cannot be completely analyzed using merely CIA, since it is exclusively based on chemical composition and corresponding alteration. The CIA can be used to quantify the weathering process in a limited range, and further considerations such as rock composition, strength characteristics will be required to configure the comprehensive weathering impact on any specific region.