• 분석과학
• /
• 제6권4호
• /
• pp.395-399
• /
• 1993

혈압 강하제로 쓰이는 생약인 조구등(Uncaria hooks)으로부터 네 가지 알칼로이드(corynoxeine, rhyncophylline, isocorynoxeine, isorhynchophylline)들을 분리하고 이들을 HPLC를 이용하여 정량하였다. 그 결과 조구등내 함량은 isocorynoxeine 0.21%, isorhynchophylline 0.16%, corynoxeine 0.28%, rhynchophylline 0.23%인 것으로 나타났다. 또한 corynoxeine과 isocorynoxeine, rhynchophylline과 isorhynchophylline은 열처리에 의하여 상호 변환하였으며 물로 열처리하여 추출하였을 때 corynoxeine과 rhynchophylline의 상대적인 양이 증가하였다.

• 약학회지
• /
• 제40권1호
• /
• pp.36-40
• /
• 1996

Chemical constituents of Uncaria hooks were studied. Four compounds were isolated from the ether soluble fraction by chromatographic purification process. They were ident ified as isocorynoxeine-N-oxide, anti-isorhynchophylline-N-oxide, physcion and ursolic acid by spectral evidences. Their contents analyzed by HPLC or GC method were physcion 0.04%, ursolic acid 0.26%, isocorynoxeine-N-oxide 0.04% and anti-isorhynchophylline-N-oxide 0.03% respectively. Isocorynoxeine-N-oxide was not reported in nature so far.

• Biomolecules & Therapeutics
• /
• 제12권3호
• /
• pp.138-144
• /
• 2004

Therapeutic effect of a Kampo medicine, Choto-san, in patients with vascular dementia was demonstrated by a double-blind and placebo-controlled clinical trial. To clarify the therapeutic efficacy of Choto-san, anti-ischemic effect in mice, hypotensive effect in spontaneously hypertensive rats (SHR), anti-oxidative effects in vitro, and N-methyl-D-aspartate (NMDA) receptor-blocking activity using Xenopus oocytes were studied. (1) Pretreatment with Choto-san (0.75-6.O g/kg, P.O.) or a component herb Chotoko (Uncaria genus: 75 - 600 mg/kg, P.O.) prevented ischemia-induced impairment of spatial learning behaviour in mice. Indole alkaloids- and phenolic fractions extracted from Chotoko also improved significantly the learning deficit. (2) Subchronic administration of Choto-san (0.5 g/kg, p.o.) caused a significant hypotensive effects in SHR. (3) Choto-san, Chotoko, and the phenolic constituent, (-) epicatechin, significantly protected the NG108-15 cell injury induced by $H_20_2$ exposure in vitro and also inhibited lipid peroxidation in the brain homogenate. (4) Indole alkaloids, rhynchophylline and isorhynchophylline (1-100 uM), reversibly reduced NMDA-induced current in the receptor-expressed Xenopus oocytes. These results suggest that anti-vascular dementia effects of Choto-san are mainly due to the effect of Chotoko. From these results, it is possible to make a novel dietary supplement through several extraction steps from Chotoko.

• Natural Product Sciences
• /
• 제11권3호
• /
• pp.145-149
• /
• 2005

A methanol soluble extract from the dried hooks and stems of Uncaria rhynchophylla showed a strong inhibitory activity against monoamine oxidase in mouse brain. Using a bioassay-guided purification of this extract, a known ${\beta}-carboline$ type alkaloid, harman (1), was obtained as an active constituent. In addition, five known indole alkaloids, isocorynoxeine (2), isorhynchophylline (3), corynoxeine (4), cadambine (5), and $3{\alpha}-dihydrocadambine$ (6), were isolated and found to be weakly active or inactive.