• The Journal of the Korean Society for Microbiology
• /
• v.22 no.2
• /
• pp.103-108
• /
• 1987

Vibrio vulnificus septicemia is nor-rare diease in Korea. Carriage rate of the orgaism by shellfish is not well known. In this study performance of SPS agar and SGP broth and effect of storage of specimen in the isolaton was determined using the shellfish specimens collected from the coast and market of Koonsan city. Isolation rate was similar with TCBS and with SPS, but the rate became much highher after enrichment in SGP broth. 80% of oyster speimes were positve when inoculated immediately, but the rate dropped rapidly after storage of specimens at freezing temperature for sometime. All of the isolates fermented lactose in 2 days. A few isolates were not identfiable with API 20E system, because of acid prduction from melibiose. Serover 04 was the frequent isolates.

• Journal of Environmental Health Sciences
• /
• v.23 no.1
• /
• pp.18-27
• /
• 1997

The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

• Korean journal of food and cookery science
• /
• v.15 no.3
• /
• pp.238-243
• /
• 1999

For the development of technique for isolation of naked barley starch from Youngsan variety, optimum conditions of the isolation process were investigated. The effect of blending was examined and the results showed that 29.7% starch yield was obtained by 6 times of blending. After the blending, the barley starch contained 3.2% protein, 0.7% fat, 0.4% fiber, 0.4% ash and 2.8% ${eta}$-glucan. The opitmum conditions of ${eta}$-glucanase treatment were studied and the results showed that the amount of ${eta}$-glucanase and barley flour-water ratio were 60,000 unit and 1/2, the optimum steeping temperature, pH were $45^{\circ}C$ and 6.5, respectively. The effect of alkali treatment which would be supposed to increase the yield and purity of the barley starch was also examined. 76.7% starch content was obtained by 2 hr of alkali treatment. After all the treatment of isolation process, the barley starch finally contained 0.2% protein and 0.1% ${eta}$-glucan.

• Earthquakes and Structures
• /
• v.13 no.3
• /
• pp.267-277
• /
• 2017

Base-isolation is now being adopted as a retrofitting strategy to improve seismic behaviour of reinforced concrete (r.c.) framed structures subjected to far-fault earthquakes. However, the increase in deformability of a base-isolated framed building may lead to amplification in the structural response under the long-duration horizontal pulses of high-magnitude near-fault earthquakes, which can become critical once the strength level of a fire-weakened r.c. superstructure is reduced. The aim of the present work is to investigate the nonlinear seismic response of fire-damaged r.c. framed structures retrofitted by base-isolation. For this purpose, a five-storey r.c. framed building primarily designed (as fixed-base) in compliance with a former Italian seismic code for a medium-risk zone, is to be retrofitted by the insertion of elastomeric bearings to meet the requirements of the current Italian code in a high-risk seismic zone. The nonlinear seismic response of the original (fixed-base) and retrofitted (base-isolated) test structures in a no fire situation are compared with those in the event of fire in the superstructure, where parametric temperature-time curves are defined at the first level, the first two and the upper levels. A lumped plasticity model describes the inelastic behaviour of the fire-damaged r.c. frame members, while a nonlinear force-displacement law is adopted for the elastomeric bearings. The average root-mean-square deviation of the observed spectrum from the target design spectrum together with a suitable intensity measure are chosen to select and scale near- and far-fault earthquakes on the basis of the design hypotheses adopted.

• Proceedings of the KSME Conference
• /
• 2001.06c
• /
• pp.275-280
• /
• 2001

Seismic isolator system is one of the most widely used base isolation system in order to control the vibration of structure against earthquake excitation. The evaluation of vulcanization time in molding the rubber bearing is very important for both proper ability of isolator and efficiency of manufacture. This paper deals with experimental measurement of temperature of isolator with senor inside in it, and compared with the result of FEA in order to evaluate the vulcanization time. Properties of rubber bearing which is used in the FEA are obtained by controlling the specific heat of rubber. With the obtained properties of rubber, the isolator is analysed by FEA. As a result, an appropriate analytical vulcanization time is obtained. This time is regarded as an appropriate temperature, which is used to effective manufacture.

• Journal of Magnetics
• /
• v.17 no.4
• /
• pp.255-260
• /
• 2012

In this study, we determine that the electron paramagnetic resonance line-width (EPRLW) is axially symmetric about the c-axis and analyze the spin Hamiltonian with an isotopic g-factor of 1.9920 at a frequency of 9.5 GHz. It should be noted that the electron paramagnetic resonance signals are Lorentzian. Our findings show that the EPRLW decreases exponentially with an increase in the temperature; i.e., its temperature dependence in the range 300-400 K obeys Arrhenius behavior, this kind of temperature dependence indicates an off-center a motional narrowing of the spectrum when $Mn^{2+}$ impurity ions substitute for $Nb^{5+}$ ions. The specific heats follow a linear dependence suggesting a simple Debye $T^3$ behavior.

• Journal of Sensor Science and Technology
• /
• v.24 no.3
• /
• pp.159-164
• /
• 2015

Solid state electrochemical and chemo-resistive gas sensors have been used widely but can operate only under high temperature. For reducing the power consumption and optimizing the structure of the substrate of these sensors, we conducted device and circuit simulations using the COMSOL Multiphysics simulator. For assessing the effective types of substrate and heat isolation, we conducted three-dimensional thermal simulations in two separate parts; (a) by changing the shape of the contacting holes and (b) punching additional holes on the substrate. Thus, it was possible to achieve high temperature in the sensor end of the substrate while maintaining low power consumption, and temperature in the circuit.

• Journal of the Korean Chemical Society
• /
• v.67 no.1
• /
• pp.28-32
• /
• 2023

The reaction conditions optimization, including the temperature of the reaction, amount of catalyst required, and reaction time for the linoleic acids (LAs) and conjugated linoleic acids (CLAs) production by catalytic dehydration of castor oil via saponification was investigated by response surface methodology (RSM). It was confirmed that all three parameters (temperature, time, and amount of catalyst) were influential factors in isolating LAs and CLAs. When the temperature was increased, the iodine value increased, and the reaction time and catalyst amount increased. The optimal reaction conditions were: 240 ℃, 2.2 h reaction time, and 7 wt% catalyst amount. The maximum iodine value reached 156.25 with 91.69% conversion to the essential fatty acids.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.13 no.6
• /
• pp.514-519
• /
• 2000

This paper describes on the temperature characteristics of a SDB(silicon-wafer direct bonding) SOI(silicon-on-insulator) Hall sensor. Using the buried oxide $SiO_2$as a dielectrical isolation layer a SDB SOI Hall sensor without pn junction has been fabricated on the Si/ $SiO_2$/Si structure. The Hall voltage and the sensitivity of the implemented SOI Hall sensor show good linearity with respect to the applied magnetic flux density and supplied current. In the temperature range of 25 to 30$0^{\circ}C$ the shifts of TCO(temperature coefficient of the offset voltage) and TCS(temperature coefficient of the product sensitivity) are less than $\pm$6.7$\times$10$_{-3}$ and $\pm$8.2$\times$10$_{-4}$$^{\circ}C$ respectively. These results indicate that the SDB SOI structure has potential for the development of a silicon Hall sensor with a high-sensitivity and high-temperature operation.

• Korean Journal of Microbiology
• /
• v.16 no.3
• /
• pp.111-121
• /
• 1978

About 40 temperature-sensitive mutants have been isolated as a preliminary step to study the spore germination, the cell cycle, and the control of macromolecular synthesis in Aspergillus nidulans. To obtain temperature-sensitive mutants rapidly and effectively, the selective enrichment method using antifungal antibiotic nystatin was developed. Based on the data which had applied to the concentration of auxotrophic mutants by the earlier investigators, the optimal concentration and the time of treatment at the nonpermissive temeprature were determined as 50 to 100 units per ml and 4.5 hr., respectively. Out of 41 ts mutants assigned to the strain symbol PK, thirteen that seemed to be arrested at the earlystage of spore germination were subjected to the further cytological and genetic analysis. Elght of these mutants are able to form germ tube and five not. Staining with acid fuchsin for the 5PK strains shows that one irreversible mutant, PK6 strain able to form germ tube, accumulate mitotic spindle, being arrested in mitosis. Another PK15 and PK23 strain have more than one intact nucleolus without germ tube formation at the restrictive temperature. the temperature-senstive mutation in PK12 strain, the onlystrain which is able occurred in certain gene specific for the germination of spore. All of the ts markers are recessive and complement each other in heterokaryon between two different ts markers at the restrictive temperature.