• Journal of Microbiology and Biotechnology
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• v.33 no.8
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• pp.1066-1075
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• 2023

Kimchi is a traditional Korean fermented vegetable that is stored and fermented at low temperatures. However, kimchi lactic acid bacteria (LAB) are typically isolated under mesophilic conditions, which may be inappropriate for isolating the diverse LAB. Therefore, this study investigated the suitable conditions for isolating various LAB from kimchi. Here, LAB were isolated from four kimchi samples using MRS, PES, and LBS media and varying isolation temperatures (30, 20, 10, and 5℃). Then, MRS was selected as the suitable medium for LAB isolation. A comparison of culture-dependent and culture-independent approaches indicated that 5℃ was not a suitable isolation temperature. Thus, the number and diversity of LAB were determined at 30, 20, and 10℃ using 12 additional kimchi samples to elucidate the effect of isolation temperature. With the exception of two samples, most samples did not substantially differ in LAB number. However, Leuconostoc gelidum, Leuconostoc gasicomitatum, Leuconostoc inhae, Dellaglioa algida, Companilactobacillus kimchiensis, Leuconostoc miyukkimchii, Leuconostoc holzapfelii, and Leuconostoc carnosum were isolated only at 10 and 20℃. The growth curves of these isolates, except Leu. holzapfelii and Leu. carnosum, showed poor growth at 30℃. This confirmed their psychrotrophic characteristics. In Weissella koreensis, which was isolated at all isolation temperatures, there was a difference in the fatty acid composition of membranes between strains that could grow well at 30℃ and those that could not. These findings can contribute to the isolation of more diverse psychrotrophic strains that were not well isolated under mesophilic temperatures.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2005.03a
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• pp.497-504
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• 2005

This paper presents the results of experimental studies on the equipment isolation effect considering the floor response. For this purpose, shaking table tests were performed. For the measuring the floor response, numerical analysis was performed. For the isolation for the equipment, Natural Rubber Bearing(NRB), High Damping Rubber Bearing(HDRB) and Friction Pendulum System(FPS) were used. Finally, it is presented that the isolation systems used in this test can be adopted for the small equipment isolation. But the rubber bearing used in this study affected to the temperature change very sensitively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1028-1032
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• 1997

Low-temperature crystallization method silver nitrate-impregnated silicic acid column chromatography were applied for the isolation of pure $\alpha$-linolenic acid(ALA) from perilla seed oil. ALA or 78% in purity(HALA; yield, 83%) was obtained from the fatty acid mixture(ALA, 65.7%) derived from perilla oil by the low-temperature crystallization method, when the mixture was frozen at -8$0^{\circ}C$ for 210min. ALA over 90% in purity(yield, 71%) was also obtained from HALA ethyl esters(ALA, 78%) by the silver nitrate-impregnated silicic acid column(100cm$\times$10cm, i.d.) chromatography. In addition, the silver nitrate-impregnated silicic acid could be semipermanently used for isolation of ALA, because $Ag^{+}$ ion was not dissociated from the stationary phase.

• Structural Engineering and Mechanics
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• v.87 no.5
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• pp.405-418
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• 2023

This study focused on the effectiveness of seismic isolation technique in case of a reinforced concrete structure with soft story defined as the stiffness irregularity between adjacent stories. In this context, a seismically isolated 3-story reinforced concrete structure was analyzed by gradually increasing the first story height (3.0, 4.5, and 6.0 m). The seismic isolation system of the structure is assumed to be composed of lead rubber bearings (LRB). In the analyses, isolators were modeled by both deteriorating (temperature-dependent analyses) and non-deteriorating (bounding analyses) hysteretic representations. The deterioration in strength of isolator is due to temperature rise in the lead core during cyclic motion. The ground motion pairs used in bi-directional nonlinear dynamic analyses were selected and scaled according to codified procedures. In the analyses, different isolation periods (Tiso) and characteristic strength to weight ratios (Q/W) were considered in order to determine the sensitivity of structural response to the isolator properties. Response quantities under consideration are floor accelerations, and interstory drift ratios. Analyses results are compared for both hysteretic representations of LRBs. Results are also used to assess the significance of the ratio between the horizontal stiffnesses of soft story and isolation system. It is revealed that seismic isolation is a viable method to reduce structural damage in structures with soft story.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.1
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• pp.67-77
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• 2008

Seismic isolation has been studied continuously as a solution of the seismic engineering to reduce the sectional forces and the damages of structures caused by earthquakes. To certify reliable design and installation of the seismic isolation systems, seismic isolation bearings should be fabricated under well planned quality control process, and proper evaluation tests for their seismic performance should be followed. In this study, shear property evaluation tests for the lead rubber bearings(LRB) and the rubber bearings(RB) were implemented and the temperature dependency tests were also implemented to evaluate the changes of shear properties according to the changes of temperature. After evaluation tests, the measured shear properties were compared to their design values and their deviation was analyzed comparing with the allowable error ranges specified in Highway Bridge Design Specifications. These results showed that a considerable number of isolation bearings have so large deviations from their design values that their error ranges were over or very close to the allowable ranges. And the test results for temperature dependency showed that the shear properties of isolation bearings would be changed in great degree by the change of temperature during their service period. If these two types of changes in their shear properties are superposed, it would possible that the changes of shear properties from their original design values are over than 50%.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.18 no.8
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• pp.759-765
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• 2005

In this paper, developed isolator for gap filler is analyzed and designed using the simulation tool. Using the designed parameters, isolator is fabricated and tested in gap filler band. Temperature characteristics of isolator depend on magnet, YIG ferrite, and conductor etc. These require temperature stability and possible method of compensation for the temperature dependent effects. The temperature stabilization tries to use Ni-alloy. Developed isolator that compare with room temperature and high temperature characteristics has change fewer than 20 MHz. Implemented isolator shows more than 20 dB isolation characteristic at center frequency(2,650 MHz) and has 0.2 dB insertion loss in overall 100 MHz operating bandwidth. Return losses of input and output port are measured below -20 dB.

• Journal of the Korean Society of Propulsion Engineers
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• v.20 no.4
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• pp.87-93
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• 2016

In a Direct Connect(DC) mode altitude engine test, a labyrinth seal is set up between an air intake duct and an engine. The labyrinth seal plays a key role in mechanically isolating them, which contributes to the accurate measurement of thrust and the other component forces. However, when high vertical temperature gradient is generated in the supplied air in the duct, the isolation breaks down. In this paper, a labyrinth seal control device is designed and installed in an effort to eliminate the issue. Test result shows the device successfully gets rid of the contact problem even when high vertical temperature gradient is produced.

• Nuclear Engineering and Technology
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• v.46 no.5
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• pp.605-618
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• 2014

The effects of variability of the mechanical properties of lead rubber bearings on the response of a seismic isolation system are investigated. Material variability in manufacturing, aging, and operation temperature is assumed, and two variation models of an isolation system are considered. To evaluate the effect of ground motion characteristics on the response, 27 earthquake record sets with different peak A/V ratios were selected, and three components of ground motions were used for a seismic response analysis. The response in an isolation system and a superstructure increases significantly for ground motions with low A/V ratios. The variation in the mechanical properties of isolators results in a significant influence on the shear strains of the isolators and the acceleration response of the superstructure. The variation provisions in the ASCE-4 are reasonable, but more strict variation limits should be given to isolation systems subjected to ground motions having low A/V ratios. For application of seismic isolation systems to safety-related nuclear structures, the variation in the material and mechanical properties of the isolation system should be properly controlled during the manufacturing and aging processes. In addition, special consideration should be given to minimize the accidental torsion caused by the dissimilarity in the stiffness variations of the isolators.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1995.05a
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• pp.227-229
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• 1995

Using thermal oxide SiO$_2$ as a dielectrical isolation layer, SOI Hall sensors without pn junction isolation have been fabricated on Si/SiO$_2$/Si structures. The SOI structure was formed by SDB (Si- wafer direct bonding) technology. The Hall voltage and the sensitivity of Si Hall devices implemented on the SDB SOI structure show good linearity with respect to the appled magnetic flux density and supplied current. The product sensitivity of the SDB SOI Hall device is average 600V/V.T. In the trmperature range of 25 to 300$^{\circ}C$, the shifts of TCO(Temperature Coefficient of the Offset Voltage) and TCS(Temperature Coefficient of the Product Sensitivity) are less than ${\pm}$ 6.7x10$\^$-3/ C and ${\pm}$8.2x10$\^$04/$^{\circ}C$, respectively. These results indicate that the SDB SOI structure has potential for the development of Hall sensors with a high-sensitivity and high-temperature operation.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2000.05b
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• pp.29-33
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• 2000

This paper describes on the temperature characteristics of a SDB(silicon-wafer direct bonding) SOI(silicon-on-insulator) Hall sensor. Using the buried oxide $SiO_2$ as a dielectrical isolation layer, a SDB SOI Hall sensor without pn junction isolation has been fabricated on the Si/$SiO_2$/Si structure. The Hall voltage and the sensitivity of the implemented SOI Hall sensor show good linearity with respect to the applied magnetic flux density and supplied current. In the temperature range of 25 to $300^{\circ}C$, the shifts of TCO(temperature coefficient of the offset voltage) and TCS(temperature coefficient of the product sensitivity) are less than ${\pm}6.7{\times}10^{-3}/^{\circ}C$ and ${\pm}8.2{\times}10^{-4}/^{\circ}C$, respectively. These results indicate that the SDB SOI structure has potential for the development of a silicon Hall sensor with a high-sensitivity and high-temperature operation.