• Journal of Institute of Control, Robotics and Systems
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• v.8 no.3
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• pp.256-263
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• 2002

Optical Multistage Interconnection Networks(OMINs) comprising photonic switches have been studied extensively as important interconnecting building blocks for communication networks and parallel computing systems. A basic element of photonic switching networks is a 2$\times$2 directional coupler with two inputs and two outputs. This paper is concerned with the diagnosis of cross-talk-faults in OMINs. As the size of today's network becomes very large, the conventional diagnosis methods based on tests and simulation have become inefficient, or even more, impractical. In this paper, we propose a simple and easily implementable algorithm for detection and isolation of the single crosstalk-fault in OMINs. Specifically, we develope an algorithm fur the isolation of the source fault in switching elements whenever the single crosstalk-fault is detected in OMINS. The proposed algorithm is illustrated by an example of 16$\times$16 banyan network.

• Toxicological Research
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• v.8 no.2
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• pp.285-302
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• 1992

Human and rat hepatocytes were isolated by nonperfusion method and cultured for longer than 5 days. Human liver biopsy sample and rat liver were used as hepatocyte source. Several physical and chemical factors which were influencing on hepatocyte isolation procedure were examined and a batch isolation procedure was established for small size sample of rat liver. Isolated hepatocytes showed normal morphlologica characteristics in microscopy and electron microscopical examinations and a morphologica response to phalloidin. Isolated cells were cultured as a monolayer and proven to have intact morphological characteristics for longer than 15 days. Because human liver sample is harder and tighter compared with rat liver, a standard procedure for rat hepatocytes was slightly modified to reduce mechanical damage. Similarly with rat hepatocytes, isolated human hepatocytes showed a normal morphological characteristics and could be cultured for longer than 15days. Human and rat hepatocytes were examined on their functional integrities including cytochrome-P450 related enzyme activity and it's inducibility, hormonal inducibility of AIB uptake and TAT activity, albumin synthesis, DNA synthesis, cellular protein maintenance. In all parameters used in the present study, human and rat hepatocytes showed normal functional characteristics.

• The Journal of the Korean Society for Microbiology
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• v.19 no.1
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• pp.73-77
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• 1984

Vibrio vulnificus is an organism capable of causing septicemia and wound infection in compromised patients. The source of infection is known to be raw oysters and others. The prevalence of the organism in Korean sea water and shellfish is not known. The authors surveyed shellfish and other specimens obtained mainly from a market in Seoul and from ones in Inchon. Five cultures of V. vulnificus were isolated from oyster and clam samples. Two isolates had typical characteristics of the strains isolated from patients, i.e., definite hemolysis and typical biochemical reactions. However, other 2 isolates were sucrose positive, although the identity were confirmed by Center for Disease Control. We do not know wether such strains are pathogenic or not. For the isolation of V. vulnificus from environmental samples, TCBS agar and VV agar were not very selective or differential. We isolated our strains with the use of OF-lactose agar and SPS agar. However OF-lactose agar did not support good growth of V. vulnificus, while SPS agar did not suppress other vibrios.

• Mycobiology
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• v.37 no.2
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• pp.114-120
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• 2009

Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination of parameters such as carbon and nitrogen source, pH and temperature. Nutritional and culture conditions for the production of antibiotics by this organism under shake-flask conditions were also optimized. Maltose (5%) and soytone (5%) were found to be the best carbon and nitrogen sources for the production of antibiotics by S. albidoflavus C247. Additionally, 62.89% mycelial growth inhibition was achieved when the organism was cultured at $30^{\circ}C$ and pH 6.5. Ethyl acetate (EtOAc) was the best extraction solvent for the isolation of the antibiotics, and 100 ${mu}$/ml of EtOAc extract was found to inhibit 60.27% of the mycelial growth of Rhizoctonia solani AG2-2(IV) when the poison plate diffusion method was conducted.

• The Korean journal of helicobacter and upper gastrointestinal research
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• v.18 no.3
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• pp.157-161
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• 2018

Liquid biopsy, the analysis of circulating biomarkers from peripheral blood, such as circulating tumor cells (CTCs) and circulating tumor DNA, and exosomes, offers a less invasive, new source of cancer-derived materials that may reflect the status of the disease better and thereby contribute to personalized treatment. Recent advances in microfluidics and molecular analysis technologies have resulted in greatly improved CTC enumeration and detection. In this article, we review commercially available technologies used to isolate CTCs from peripheral blood, including immunoaffinity and label-free, physical property-based isolation methods. Although enormous technological progress has been made, especially within the last decade, only a few CTC detection methods have been approved for routine clinical use. Here, we provide an overview of the current CTC isolation methods and examples of their potential application for early diagnosis, prognosis, treatment monitoring, and prediction of resistance to cancer therapy. Furthermore, the challenges that remain to be addressed before such tools are implemented for routine use in clinical settings are discussed.

• Biomedical Science Letters
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• v.27 no.4
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• pp.310-316
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• 2021

Mobile phones used by healthcare workers are not only an indicator of the contamination of healthcare associated bacteria, but can also be another source of infection. The number and time of handwashing, mobile phone operation time and disinfection were highly relation with the bacterial contamination on the surface of mobile phone. Healthcare associated bacteria isolated from the mobile phone surface were 28 MRCoNS (48.3%), 14 S. aureus (24.2%), 3 MRSA (5.2%), 5 A. baumannii (8.6%), 3 MRAB (5.2%), 3 Entrococcus spp. (5.1%), 2 Pantoea spp. (3.4%), 2 A. lowffii (3.4%), 1 E. cloacae (1.7%), 1 P. stutzeri (1.7%), and P. mirabillis (1.7%). For isolation according to department, 2 MRAB from the emergency room and 1 MRSA from intensive unit, the radiology team and the rehabilitation medical team, respectively were isolated. As a result of the relation of isolates from the department of patient contact (ER, RT, GW, CP, ICU, RMT), the bacterial isolation rate was 75% and the department of patient non-contact (MRT) was 10%.

• Korean Circulation Journal
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• v.53 no.5
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• pp.273-293
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• 2023

Pulsed field ablation (PFA) was recently rediscovered as an emerging treatment modality for the ablation of cardiac arrhythmias. Ultra-short high voltage pulses are leading to irreversible electroporation of cardiac cells subsequently resulting in cell death. Current literature of PFA for pulmonary vein isolation (PVI) consistently reported excellent acute and long-term efficacy along with a very low adverse event rate. The undeniable benefit of the novel ablation technique is that cardiac cells are more susceptible to electrical fields whereas surrounding structures such as the pulmonary veins, the phrenic nerve or the esophagus are not, or if at all, minimally affected, which results in a favorable safety profile that is expected to be superior to the current standard of care without compromising efficacy. Nevertheless, the exact mechanisms of electroporation are not yet entirely understood on a cellular basis and pulsed electrical field protocols of different manufactures are not comparable among one another and require their own validation for each indication. Importantly, randomized controlled trials and comparative data to current standard of care modalities, such as radiofrequency- or cryoballoon ablation, are still missing. This review focuses on the "pearls" and "pitfalls" of PFA, a technology that has the potential to become the future leading energy source for PVI and beyond.

• Journal of Home Health Care Nursing
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• v.31 no.1
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• pp.56-65
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• 2024

Purpose: This study aimed to explore the association between demographic characteristics, hospitalization-related characteristics, and the severity of long-term hospitalization in a high-level general hospital, and to analyze the factors influencing decisions of all patients. Methods: General and clinical characteristics of the participants were analyzed using frequency, percentage, mean, and standard deviation. Differences in these characteristics, contingent upon whether a power source was requested, were analyzed using independent t-Test and Chi-squared tests. Logistic regression analysis was used to identify the factors related to the presence or absence of power requests. Results: The factors impacting the decision to refer a dependent variable include medical treatment (neurosurgery) (B=2.118, SE=0.960, p-value=.027, OR=8.314, 95% CI=1.267-54.551), infection isolation (CRE) (B=1.336, SE=0.666, p-value=.045, OR=3.804, 95% CI=1.032-14.021), and the utilization of tertiary antibiotics (B=3.076, SE=1.362, p-value= .024, OR=21.663, 95% CI=1.502-312.530). Conclusion: This study found a significant association between medical treatment (neurosurgery), infection isolation (CRE), and the use of tertiary antibiotics as dependent variables. These findings indicate that continuous monitoring can contribute to a reduction in long-term financial burdens.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• v.11B no.3
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• pp.112-118
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• 2001

In the near future, the voltage of power system for passenger vehicle will be changed to 42V from existing 14V./ Because of increasing power and voltage ratings used in the vehicle the motor drive system has high switching dv/dt and it generates electromagnetic interference (EMI) To solve these problems multi-level inverter system may be used The feature of multi-level inverter is the output voltage to be synthesized from several levels of voltage Because of this feature high switching dv/dt and EMI can be reduced in the multi-level inverter system But as the number of level is increased manufacturing cost is getting expensive and system size is getting large. Because of these disadvantages the application of multi-level inverter has been restricted only to high power drives. The method to reduce manufacturing cost and system size is to integrate circuit of multi-level inverter into a few chips But isolated power supply and signal isolation circuit using transformer or opto-coupler for drive circuit are obstacles to implement the integrated circuit (IC) In this paper a drive circuit of 4-level inverter suitable for integration to hybrid or one chip is proposed In the proposed drive circuit DC link voltage is used directly as the power source of each gate drive circuit NPN transistors and PNP transistors are used to isolate to transfer the control signals. So the proposed drive circuit needs no transformers and opto-couplers for electrical isolation of drive circuit and is constructed only using components to be implemented on a silicon wafer With th e proposed drive circuit 4- level inverter system will be possible to be implemented through integrated circuit technology Using the proposed drive circuit 4- level inverter system is constructed and the validity and characteristics of the proposed drive circuit are proved through the experiments.

• Food Science of Animal Resources
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• v.35 no.4
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• pp.459-465
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• 2015

Bovine milk provides essential nutrients, including immunologically important molecules, as the primary source of nutrition to newborns. Recent studies showed that RNAs from bovine milk contain immune-related microRNAs (miRNA) that regulate various immune systems. To evaluate the biological and immunological activity of miRNAs from milk products, isolation methods need to be established. Six methods for extracting total RNAs from bovine colostrums were adopted to evaluate the isolating efficiency and expression of miRNAs. Total RNA from milk was presented in formulation of small RNAs, rather than ribosomal RNAs. Column-combined phenol isolating methods showed high recovery of total RNAs, especially the commercial columns for biofluid samples, which demonstrated outstanding efficiency for recovering miRNAs. We also evaluated the quantity of five immune-related miRNAs (miR-93, miR-106a, miR-155, miR-181a, miR-451) in milk processed by temperature treatments including low temperature for long time (LTLT, 63℃ for 30 min)-, high temperature for short time (HTST, 75℃ for 15 s)-, and ultra heat treatment (UHT, 120-130℃ for 0.5-4 s). All targeted miRNAs had significantly reduced levels in processed milks compared to colostrum and raw mature milk. Interestingly, the amount of immune-related miRNAs from HTST milk was more resistant than those of LTLT and UHT milks. Our present study examined defined methods of RNA isolation and quantification of immune-specific miRNAs from small volumes of milk for use in further analysis.