• Title/Summary/Keyword: isolation performance

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ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF AN IMMOBILIZED BACTERIUM PRODUCING N2 FROM NH4+ UNDER AN AEROBIC CONDITION

  • Park, Kyoung-Joo;Cho, Kyoung-Sook;Kim, Jeong-Bo;Lee, Min-Gyu;Lee, Byung-Hun;Hong, Young-Ki;Kim, Joong-Kyun
    • Environmental Engineering Research
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    • v.10 no.5
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    • pp.213-226
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    • 2005
  • To treat wastewater efficiently by a one-step process of nitrogen removal, a new bacterial strain producing $N_2$ gas from ${NH_4}^+$ under an aerobic condition was isolated and identified. The cell was motile and a Gram-negative rod, and usually occurred in pairs. By 16S-rDNA analysis, the isolated strain was identified as Enterobacter asburiae with 96% similarity. The isolate showed that the capacity of $N_2$ production under an oxic condition was approximately three times higher than that under an anoxic condition. Thus, the consumption of ${NH_4}^+$ by the isolate was significantly different in the metabolism of $N_2$ production under the two different environmental conditions. The optimal conditions of the immobilized isolate for $N_2$ production were found to be pH 7.0, $30^{\circ}C$ and C/N ratio 5, respectively. Under all the optimum reaction conditions, $N_2$ production by the immobilized isolate resulted in reduction of ORP with both the consumption of DO and the drop of pH. The removal efficiencies of $COD_{Cr}$, and TN were 56.1 and 60.9%, respectively. The removal rates of $COD_{Cr}$, and TN were the highest for the first 2.5 hrs with the removal $COD_{Cr}/TN$ ratios of 32.1, and afterwards the rates decreased as reaction proceeded. For application of the immobilized isolate to a practical process of ammonium removal, a continuous operation was executed with a synthetic medium of a low C/N ratio. The continuous bioreactor system exhibited a satisfactory performance at 12.1 hrs of HRT, in which the effluent concentrations of ${NH_4}^+$-N was measured to be 15.4 mg/L with its removal efficiency of 56.0%. The maximum removal rate of ${NH_4}^+$-N reached 1.6 mg ${NH_4}^+$-N/L/hr at 12.1 hrs of HRT(with N loading rate of $0.08\;Kg-N/m^3$-carrier/d). As a result, the application of the immobilized isolate appears a viable alternative to the nitrification-denitrification processes.

A Study on Mastitis Infection Rate and Investigation of Milk Contamination Level by Measuring the Bacteria and Somatic Cell Counts in Gyunggi Area (京畿一圓 乳牛의 乳房炎 感染率과 原乳의 細菌 및 體細胞數 測定에 따른 乳質 汚染度에 관한 연구)

  • Eu, Byung Woo
    • Journal of Environmental Health Sciences
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    • v.11 no.2
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    • pp.41-54
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    • 1985
  • This study was conducted for the improvement of milk quality and milk hygiene in public health point of view. Investigation of mastiris infection rate, isolation and identification of causative microorganisms in CMT positive milk, investigation of milk contamination level by measuring the bacteria and soma tic cell counts and investigation of dairy management in farms were performed on 1.605 quarters milk of 434 cows of 20 dairy farms in Gyunggi-area from September 1983 to March 1984. The results were summarized as follows 1. Sixteen (3.7%) of 434 cows were found to be infected with clinical mastiris. 234 (53.9%) of 434 cows and 608(37.9%) of 1, 605 quarters were found to be infected with subclinical mastiris. 2. The causative microorganisms isolated were Staphylococcus aureus (38.3%), Staphylococcus epidermidis(21.0%), Micrococci(13.6%), Streptococcus spp. (12.3%), E. coli (7.4%), Fungus & Yeast (1.6%) and others (5.8%). 3. Total numbers of bacteria were $9.2{\times}10^6$ to $1.21{\times}10^7/ml$(av. $1.805{\times} 10^7/ml$), numbers of coliform bacteria were $4.1{\times} 10^5$ to $9.4{\times} 10^5$/ml(av. $7.05{\times} 10^5$/ml) and somatic cell counts were $4.8{\times} 10^5$ to $1.52{\times} 10^6$ cells/ml (av. $9.5{\times} 10^5$cells/ml) in bulk milk. 4. As comparing with CMT score of +, ++ and +++, somatic cell counts were $3.4{\times} 10^5$ to $1.64{\times} 10^6$ cells/ml (av. $6.41{\times} 10^5$cells/ml), $5.4{\times} 10^5$ to $2.75{\times} 10^6$ cells/ml(av. $1.762{\times} 10^6$cells/ml) and $1.97{\times} 10^6$ to $9.75{\times} 10^6$ cells/ml(av. $7.781{\times} 10^6$cells/ml), respectively. 5. In investigation on dairy management, performance of dry cow therapy, teat dipping after milking, disinfection of milking machine at every milking, replacement of milk liner within 6months and opportunity of acquirement for the mastiris control techniques by dairy education were 65%, 40%, 45%, 55% and 50% in 20 dairy farms, respectively.

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Characteristics Measurement of Hyperelastic SMA Gear for Micro-jitter Attenuation of X-band Antenna of Compact Advanced Satellite (차세대중형위성 적용가능성 검토를 위한 X-band 안테나의 미소진동 저감용 초탄성 SMA 기어의 특성 측정)

  • Jeon, Young-Hyeon;Back, Hyeon-Gyu;Song, Da-Il;Kang, Eun-Su;Oh, Hyun-Ung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.45 no.9
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    • pp.784-793
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    • 2017
  • A two-axis gimbal-type X-band antenna mounted on an observation satellite can efficiently transmit high-capacity image data to a ground station regardless of both the satellite position and the orbital motion. However, this X-band antenna induces unnecessary micro-jitter which can degrade the image quality of the high-resolution observation satellite. Therefore, to achieve the high-resolution image quality from the observation satellite, micro-jitters have been required to be isolated. In this study, to resolve aforementioned drawback, we proposed blade gear using a shape memory alloy (SMA) applied to azimuth stage of X-band antenna. To investigate the rotational basic characteristics of the proposed SMA blade gear, we performed rotational static loading test. Futhermore, to evaluate the cycle to failure of the gear, accelerated life test was conducted. The temperature test was conducted to confirm rotational basic characteristics at various temperature conditions. To verify the isolation performance for micro-jitter, we performed micro-jitter measurement test.

Study on the Physical Property of Thermal Curtains for Greenhouse (시설하우스용 보온커튼재의 물리적 특성에 관한 연구)

  • 장유섭;오권영;김승희;전종길;강금춘;정두호
    • Journal of Bio-Environment Control
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    • v.5 no.1
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    • pp.34-42
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    • 1996
  • This study was conducted to investigate the physical and optical properties of polypropylene and polyester thermal curtains, in which tensile strength, heat reservance and light transmission of two different materials were measured. The results from this study are as follows. 1. The tensile weight of different materials were ranged from 3.4kg to 13.4kg, according to the thickness of materials, but that no difference in the tensile strength was appeared between the two materials. The Elongation of polypropylene materials and the tensile weight and strength of polyester materials were greater than any other materials. 2. The light transmittances of two materials were ranged from 50.3% to 81.7 %, light transmittances in polypropylene were higher by 20-30%,than those in polyester. 3. The heat reservances of two materials were ranged from 18.2% to 41.2%, in which polypropylene showed better performance than polyester. 4. From the results of the test, the polypropylene thermal material was better in elongation, heat reservances and light transmittances, but polyester thermal material was better in tensile strength and light isolation than the other material.

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An Experimental Study on Flow Distributor Performance with Single-Train Passive Safety System of SMART-ITL (SMART-ITL 1 계열 피동안전계통을 이용한 유동분사기 성능에 대한 실험연구)

  • Ryu, Sung Uk;Bae, Hwang;Yang, Jin Hwa;Jeon, Byong Guk;Yun, Eun Koo;Kim, Jaemin;Bang, Yoon Gon;Kim, Myung Joon;Yi, Sung-Jae;Park, Hyun-Sik
    • Journal of Energy Engineering
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    • v.25 no.4
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    • pp.124-132
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    • 2016
  • In order to estimate the effect of flow distributors connected to an upper nozzle of CMT(Core Makeup Tank) on the thermal-hydraulic characteristics in the tank, a simplified 2 inch Small Break Loss of Coolant Accident(SBLOCA) was simulated by skipping the decay power and Passive Residual Heat Removal System(PRHRS) actuation. The CMT is a part of safety injection systems in the SMART (System Integrated Modular Advanced Reactor). Each test was performed with reliable boundary conditions. It means that the pressure distribution is provided with repeatable and reproducible behavior during SBLOCA simulations. The maximum flow rates were achieved at around 350 seconds after the initial opening of the isolation valve installed in CMT. After a short period of decreased flow rate, it attained a steady injection flow rate after about 1,250 seconds. This unstable injection period of the CMT coolant is due to the condensation of steam injected into the upper part of CMT. The steady injection flow rate was about 8.4% higher with B-type distributor than that with A-type distributor. The gravity injection during hot condition tests were in good agreement with that during cold condition tests except for the early stages.

Matrix solid phase dispersion isolation and high performance liquid chromatographic determination of five benzimidazole anthelmintics in bovine muscle, liver and omasum (시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석)

  • Kim, Chung-Hui;Kim, Gon-Sup;Park, Jung-Hee;Hah, Dae-Sik;Ryu, Jae-Doo;Son, Sung-Gi;Heo, Jung-Ho;Jung, Myung-Ho;Kim, Jong-Shu
    • Korean Journal of Veterinary Research
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    • v.42 no.2
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    • pp.171-181
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    • 2002
  • Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{\mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{\ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{\ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{\pm}0.007$ to $0.996{\pm}0.005$) with average relative percentage recoveries from $62.1{\pm}3.8(%)$ to $92.3{\pm}7.5(%)$ for the concentration range($0.2{\sim}6.4{\mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.

A Reconfigurable Active Array Antenna System with Reconfigurable Power Amplifiers Based on MEMS Switches (MEMS 스위치 기반 재구성 고출력 증폭기를 갖는 재구성 능동 배열 안테나 시스템)

  • Myoung, Seong-Sik;Eom, Soon-Young;Jeon, Soon-Ik;Yook, Jong-Gwan;Wu, Terence;Lim, Kyu-Tae;Laskar, Joy
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.4
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    • pp.381-391
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    • 2010
  • In this paper, a novel frequency reconfigurable active array antenna(RAA) system, which can be reconfigurable for three reconfigurable frequency bands, is proposed by using commercial RF MEMS switches. The MEMS switch shows excellent insertion loss, linearity, as well as isolation. So, the system performance degradation of the reconfigurable system by using MEMS switches can be minimized. The proposed frequency reconfigurable active antenna system is consisted with the noble frequency reconfigurable front-end amplifiers(RFA) with the simple reconfigurable impedance matching circuits(RMC), reconfigurable antenna elements(RAE), as well as a reconfiguration control board(RCB) for MEMS switch control. The proposed RAA system can be reconfigurable for three frequency bands, 850 MHz, 1.9 GHz, and 3.4 GHz, with $2{\times}2$ array of the RAE having broadband printed dipole antenna topology. The validity of the proposed RFA as well as RAA is also presented with the experimental results of the fabricated systems.

Direct-fed Microbials for Ruminant Animals

  • Seo, Ja-Kyeom;Kim, Seon-Woo;Kim, Myung-Hoo;Upadhaya, Santi D.;Kam, Dong-Keun;Ha, Jong-K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.12
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    • pp.1657-1667
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    • 2010
  • Direct-fed microbials (DFM) are dietary supplements that inhibit gastrointestinal infection and provide optimally regulated microbial environments in the digestive tract. As the use of antibiotics in ruminant feeds has been banned, DFM have been emphasized as antimicrobial replacements. Microorganisms that are used in DFM for ruminants may be classified as lactic acid producing bacteria (LAB), lactic acid utilizing bacteria (LUB), or other microorganisms including species of Lactobacillus, Bifidobacterium, Enterococcus, Streptococcus, Bacillus and Propionibacterium, strains of Megasphaera elsdenii and Prevotella bryantii and yeast products containing Saccharomyces and Aspergillus. LAB may have beneficial effects in the intestinal tract and rumen. Both LAB and LUB potentially moderate rumen conditions and improve feed efficiency. Yeast DFM may reduce harmful oxygen, prevent excess lactate production, increase feed digestibility, and improve fermentation in the rumen. DFM may also compete with and inhibit the growth of pathogens, stimulate immune function, and modulate microbial balance in the gastrointestinal tract. LAB may regulate the incidence of diarrhea, and improve weight gain and feed efficiency. LUB improved weight gain in calves. DFM has been reported to improve dry matter intake, milk yield, fat corrected milk yield and milk fat content in mature animals. However, contradictory reports about the effects of DFM, dosages, feeding times and frequencies, strains of DFM, and effects on different animal conditions are available. Cultivation and preparation of ready-to-use strict anaerobes as DFM may be cost-prohibitive, and dosing methods, such as drenching, that are required for anaerobic DFM are unlikely to be acceptable as general on-farm practice. Aero-tolerant rumen microorganisms are limited to only few species, although the potential isolation and utilization of aero-tolerant ruminal strains as DFM has been reported. Spore forming bacteria are characterized by convenience of preparation and effectiveness of DFM delivery to target organs and therefore have been proposed as DFM strains. Recent studies have supported the positive effects of DFM on ruminant performance.

The Design of an Auto Tuning PI Controller using a Parameter Estimation Method for the Linear BLDC Motor (선형 추진 BLDC 모터에 대한 파라미터 추정 기법을 이용하는 오토 튜닝(Auto Tuning) PI 제어기 설계)

  • Cha Young-Bum;Song Do-Ho;Koo Bon-Min;Park Moo-Yurl;Kim Jin-Ae;Choi Jung-Keyng
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.4
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    • pp.659-666
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    • 2006
  • Servo-motors are used as key components of automated system by performing precise motion control as accurate positioning and accurate speed regulation in response to the commands from computers and sensors. Especially, the linear brushless servo-motors have numerous advantages over the rotary servo motors which have connection with the friction induced transfer mechanism such as ball screws, timing belts, rack/pinion. This paper proposes an estimation method of unknown motor system parameters using the informations from the sinusoidal driving type linear brushless DC motor dynamics and outputs. The estimated parameters can be used to tune the controller gain and a disturbance observer. In order to meet this purpose high performance Digital Signal Processor, TMS320F240, designed originally for implementation of a Field Oriented Control(FOC) technology is adopted as a controller of the liner BLDC servo motor. Having A/D converters, PWM generators, rich I/O port internally, this servo motor application specific DSP play an important role in servo motor controller. This linear BLDC servo motor system also contains IPM(Intelligent Power Module) driver and hail sensor type current sensor module, photocoupler module for isolation of gate signals and fault signals.

Isolation and Purification of Berberine in Cortex Phellodendri by Centrifugal Partition Chromatography (Centrifugal Partition Chromatography에 의한 황백으로부터 Berberine의 분리 및 정제)

  • Kim, Jung-Bae;Bang, Byung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.27 no.3
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    • pp.532-537
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    • 2014
  • Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections, and infections of the body surface. Many analytical methods have been used to study oriental herbal medicines, such as thin-layer chromatography, column liquid chromatography, and high performance liquid chromatography (HPLC). In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out in order to separate pure compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 mL/min in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. The $^1H$ NMR spectrum revealed that the resonances at ${\delta}$ 4.10 and 4.20 ppm corresponded to three protons ($-OCH_3$), whereas those at ${\delta}$ 6.10 ppm corresponded to two protons ($-OCH_2O-$). Further, two aromatic protons (H-11 and H-12) conveys a doublet-doublet pattern. The H-11 doublet and H-12 doublet appear at ${\delta}$ 7.98 and 8.11, respectively. The $^{13}C$ NMR. spectrum showed a tetrasubstituted with a methylenedioxy group at C2 and C3, and two methoxy groups at C9 and C10. The chemical structure of the berberine was identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.