• Title/Summary/Keyword: isolation of antibiotic

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Isolation, Physico-chemical Properties and Biological Activity of Aurodox Group Antibiotics

  • Kim, Si-Kwan;Yeo, Woon-Hyung;Kim, Sang-Seock
    • Journal of Microbiology and Biotechnology
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    • v.6 no.4
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    • pp.265-269
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    • 1996
  • An isolate of Streptomyces rochei synonym was found to produce antibiotics with narrow anti-microbial spectrum against Streptococcus and Xanthomonas sp. Among the antibiotic complex produced by the strain, the main active compound was isolated, and its physico-chemical properties and biological activities were investigated. Molecular weight of the compound was determined to be ${[M+H]}^+$ 797 (FAB-MS). UV, $^1H \;and\;^{13}C$ NMR, and IR spectra suggested that the compound is a kirromycin-like aurodox group antibiotic. However, the anti-microbial spectrum of the main compound was slightly different from that of kirromycin. In addition, it was newly found that kirromycin showed a selective anti-microbial activity against Streptococcus pyogenes and phytopathogenic Xanthomonas sp.

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Selection of High Yielding Mutant Strains for the Antifungal Antibiotics KRF-001 (항진균물질 KRF-001의 고생산성 변이주 분리)

  • 이항우;김무경
    • KSBB Journal
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    • v.9 no.4
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    • pp.378-384
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    • 1994
  • An improved method for the selective isolation of high-yielding mutant strains for the production of antifungal antibiotic KRF-001 was investigated. The mutant strain U. V 4, which produces high titer of KRF-001, was selected on the high potency agar plate after ultraviolet light irradiation. The U. V 4 strain produced 2-fold more KRF-001 than the mother strain in production media. Large scale fermentation was performed using the U. V 4 strain in 100$\ell$ fermenter. The antifungal antibiotic KRF-001 secreted into culture broth was detected by HPLC in 24hrs of fermentation.

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Isolation and Biochemical Properties of Pasteurella multocida from the pneumonic lungs of swine in Cheju (제주지방 돼지의 폐병변으로부터 Pasteurella multocida 분리 및 생화학적 특성)

  • 김옥녀;이두식;문호규;김우택;서문현;배종희;임윤규;조길재
    • Korean Journal of Veterinary Service
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    • v.18 no.2
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    • pp.124-132
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    • 1995
  • The present study was conducted to investigate the incidence of Pasteurella multocida infection in cheju swine from March 1994 to December 1994 isolated organisms were identified by the biochemical properties, cellulose serological type and antibiotic susceptibilities. Pasteurella multocida was isolated from the Lungs of 96 pigs with pneumonia(51 %) among 188 slaughtered pigs. The majority of p multocida isolates were identical to those of the standard strains. On the classification of the capsular type of the isolated p multocida it consist of the 88 isolates of type A(91.6%) 2 isolates of type D (2%) and un classified 6 types(6.2%). The majority of the 96 isolates of p multocida highly susceptible to the antibiotics including ampicillin(Am), cephalotin(Ce), erythromycin(Em), gentamycin(Gm), kanamycin(Km), lincomycin(Lm), neomycin(Nm), penicillin(Pc), streptomycin(Sm), solfametoxazol/trimethoprim(Sxt) and tetracycline(Te)

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Screening and Isolation of Antibiotic Resistance Inhibitors from Herb Materials-Resistance Inhibition of Volatile Components of Korean Aromatic Herbs

  • Lee, Chung-Kyu;Kim, Hye-Kyung;Moon, Kyung-Ho;Shin, Kuk-Hyun
    • Archives of Pharmacal Research
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    • v.21 no.1
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    • pp.62-66
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    • 1998
  • The resistance inhibitory activities of 54 odorant mixtures (essential oil) from 41 Korean aromatic herbs were tested against multi-drug resistant Staphylococcus aureus SA2, which has resistances to 10 usual antibiotics including chloramphenicol. As results, combinations of 28 kinds of samples from 21 herbs and chloramphenicol have resistance inhibitory activities in dose dependent manner.

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Selective Medium for the Isolation and Counting of Bifidobacteria in Dairy Products (유제품으로부터 Bifidobacteria의 선발 및 계수를 위한 선택배지)

  • Shin, Myeong-Su;Lee, Jeong-Jun;Suh, In-Yeong;Na, Seog-Hwan;Baek, Young-Jin
    • Microbiology and Biotechnology Letters
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    • v.22 no.2
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    • pp.210-216
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    • 1994
  • Phage utilizing medium and BL agar supplemented with antibiotic Tc(tetracycline) were developed as selective media for the isolation and counting of bifidobacteria in dairy products. The former was based on the host specificity of phage. When bifidobacteria and Lactobacillus casei HY 2782 were mixed together in dairy product, L. casei HY 2782 was laysed by J1 phage which has host specificity to L. casei HY 2782 whereas bifidobacteria grew well on the selective medium added wIth J1 phage. The latter was found to inhibit the growth of S. salivarius subsp. thermophilus, L. acidophilus, and L. casei, but commercial bifidobacteria grew well in Tc-containing BL agar.

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Studies on Antibiotic Producers of Korean Soil Microbes (IV) -Isolation and Antibiotic Activity of Streptomyces Strain DMC-42- (한국(韓國) 토양균(土壤菌)중 항생물질(抗生物質) 생성균(生成菌)에 관한 연구(硏究) 제 4 보(第4報) -스트렙토마이세스속(屬) 균주(菌株) DMC-42의 분리(分離) 및 그 항균작용(抗菌作用)-)

  • Kim, Hwa-Ki;Kim, Jung-Woo;Kim, Ha-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • The Korean Journal of Mycology
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    • v.13 no.2
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    • pp.89-97
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    • 1985
  • To find antibacterial strains of the soil microorganisms in Korea, they were isolated from the soil samples of different locations and screened for antibacterial activity against several standard microorganisms. An isolate among them had antibacterial activities against gram-positive and gram-negative bacteria. The examination of its morphological, biochemical, cultural and physiological characteristics according to the International Streptomyces Project methods showed that it belongs to the genus Streptomyces. This strain appears to be a novel strain when it was compared with those species of the genus which have been so far reported. The antibiotic metabolite was produced in the submerged culture of the strain. This metabolite was extracted from the culture filtrate and purified by ion-exchange column chromatography. Physico-chemical properties of the antibacterial metabolite were characterized.

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Isolation, identification, and culture conditions of the strain producing antibacterial antibiotic (항세균성 항생물질을 생산하는 미생물의 분리, 동정 및 배양조건)

  • Yoo, Jae-Hong;Yoon, Sang-Hong;Koo, Bon-Sung;Yeo, Yun-Soo;Park, In-Cheol;Lee, Byung-Moo;Ryu, Jin-Chang
    • The Korean Journal of Pesticide Science
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    • v.3 no.2
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    • pp.1-7
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    • 1999
  • The strain with antibacterial activity was isolated among soil samples collected in Suwon area. The isolated strain was identified as Bacillus sp. YR-1 with respect to its morphological, cultural, and physiological characteristics. Optimal medium for the highest production of antibiotic was composed of sucrose 2.0%(w/v), peptone 2.0%(w/v) and NaCl 0.1%(w/v). The maximum production of antibiotic was shown at $35^{\circ}C$ for 48 hours with the initial pH 7.0.

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