• Title/Summary/Keyword: isolation, identification

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Reverse-Phase HPLC Method for Identification of Diastereomeric Constituents from Sasa borealis (Sasa borealis의 Diastereomeric 성분들의 역상 고속액체크로마토그래프 분석방법)

  • Jeong Yeon Hee;Lee Jun;Kwon Youngjoo;Seo Eun-Hyoung
    • YAKHAK HOEJI
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    • v.50 no.1
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    • pp.21-25
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    • 2006
  • Reiterated normal-phase column chromatography lead to the isolation and purification of six known compounds but for the first time from the whole plant of Sasa borealis (Hack.) Makino (Gramineae): tricin 4'-O-(erythro-${\beta}$-guaia-cylglyceryl) ether (1), tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether (2), tricin 4'-O-[erythro-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (3), tricin 4'-O-[threo-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (4), (-)-pinoresinol (5), and vanillin (6). The structures of the compounds (1-6) were established based on interpretation of high resolution NMR (COSY, HSQC, HMBC, and NOESY) spectral data. In particular, compounds 1 and 3 were diastereomers of compounds 2 and 4, respectively. These two sets of diastereomers were able to be simultaneously identified and quantified by a gradient reversed-phase HPLC method with UV photodiode array, This sensitive HPLC method is noteworthy as a simultaneous separation and identification method to test the extract of the family Gramineae which contains these compounds.

SAW ID Tag and Receiver System for Passive RFID System Application (수동형 RFID 시스템 적용을 위한 SAW ID 태그 및 수신 시스템 구현)

  • Kim, Jae-Kwon;Park, Joo-Yong;Burm, Jin-Wook
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.4
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    • pp.64-71
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    • 2008
  • SAW (Surface Acoustic Waves) ID (identification) tags have been designed and implemented for RFID (Radio frequency IDentification) systems. With SAW ID tag of pulse position encoding method, the data capacity increased 3 times compared with SAW ID tag of amplitude on/off method. Two different kinds of SAW ID tag receiver systems, heterodyne and homodyne receiver systems, were made. The direct conversion receiver showed better isolation property, 10 dB improvement than the heterodyne receiver to increase wireless interrogation distance.

Isolation and Morphological Identification of Apple Anthracnose Fungus of Colletotrichum sp. KV-21

  • Bajpai, Vivek K.;Choi, Seak-Won;Cho, Moon-Soo;Kang, Sun-Chul
    • Korean Journal of Environmental Agriculture
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    • v.28 no.4
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    • pp.442-446
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    • 2009
  • This study was undertaken to isolate and to identify a fungal pathogen Colletotrichum sp. KV-21 associated with apple anthracnose. Rotted Gala apples were used for the isolation of the fungus. The infected tissues were sterilized with 70% ethanol, washed with sterilized distilled water and were transferred to 50 ml containing potato broth (PDB) flasks. The peripheral hyphae of the fungal colony which developed from the infected tissues were isolated on to potato dextrose agar (PDA). On PDA plates the fungus grew well at $25^{\circ}C$ and occupied more than half of a 9 cm petri dish within 5 days. The fungal cultures on PDA were used for morphological observation and identification of the fungus. Conidiophores were produced on the gray to whitish sporodochial structures scattered on PDA plates which gave rise to conidiogenous cells. The structures of the conidia produced on PDA plates were subcylindrical to obovoid, fusoid, tapered and 4 to $6\;{\mu}m$ in size.

Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea (대전광역시와 충청남도 밭 토양으로부터 야생효모의 분리 및 동정)

  • Han, Sang-Min;Han, Jae-Won;Bae, Sang-Min;Park, Won-Jong;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.44 no.1
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    • pp.1-7
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    • 2016
  • This study focused on isolation and identification of wild yeasts from soils in fields near mountains and elucidation of its yeast distribution. Several kinds of yeasts were isolated from various soils of Daejeon metropolitan city and Chungcheongnam-do in Korea and identified by BLAST search of nucleotide sequences of internal transcribed spacer (ITS) region including 5.8S rRNA and D1/D2 region of 26S rDNA. Ninety-seven strains of 20 species from 61 soil samples were isolated, of which Cryptococcus podzolicus (11 strains), Debaryomyces hansenii (6 strains), and Trichosporon asahii (6 strains) were dominant species.