• Journal of Ginseng Research
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• 제21권3호
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• pp.147-152
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• 1997

Ginsenosides $Rh_1$ and $Rh_2$ Induced the differentiation of F9 teratocarcinoma stem cells. These agents are structurally similar to the steroid hormones, therefore, we speculated that the steroid receptor (s) or novel nuclear receptor (s) could be involved in the differentiation process induces by them. Based on this speculation, we tried to alone new nuclear receptors with reverse transcription-polymerase chain reaction (RT-PCR) method by isolating RNA from F9 teratocarcinoma cells induced by ginsenosides. By using RT-PCR with degenerated primers from highly conserved DNA binding domain of nuclear receptors, we identified several nuclear receptors. In northern blot analysis we found that these clones are transcriptionally regulated by ginsenoside Rhl or Rh2 treatment. Further characterizations of these clones are needed to identify the mechanism of gene expression, which has an important role in the differentiation of F9 cells induced by ginsenosides.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.422-425
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• 2001

For isolating and identifying Bifidobacterium spp. originating from Korea, feces were sampled from healthy Korean infants nursery school and postpartum care center. Through the use of gram staining and microscopic examination for cell morphology, 87 bacterial strains presumed to be the Bifidobacterium strains were isolated from 59 Koreans. To identify the Bifidobacterium strains at the genus level, these bacteria were then analyzed using the TLC method. As a result, 29 of the isolated strains were confirmed as members of the genus Bifidobacterium. 29 Bifidobacterium strains were tested acid, bile salts and oxygen tolerance and investigated antioxidative effect specially. And determined the superiority of 5 strains out of 29 Bifidobacterium strains. Finally, the selected bifidobacterium was identified with using designed 16S-ITS rDNA primer.

• Structural Engineering and Mechanics
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• 제46권3호
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• pp.371-385
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• 2013

Vibration isolators and anti-vibration mounts are ideal, for example, in creating floating floors for gymnasiums, or performance spaces. However, it is well-known that there are great difficulties on isolating vibration transmission in structural steel components, especially steel floors. Besides, the selection of inertia blocks, which are usually used by engineers as an effective vibration control measure, is usually based on crude methods or the experience of the engineers. Thus, no simple method or indices have been available for assessing the effect of inertia blocks on vibration isolation or stability of vibratory systems. Thus, the aims of this research are to provide further background description using a FE model and present and implement a modal approach, that was validated experimentally, the latter assisting in providing improved understanding of the vibration transmission phenomenon in steel buildings excited by a velocity-source type of excitation. A better visualization of the mean-square velocity distribution in the frequency domain is presented using the concept of modal expansion. Finally, the variation of the mean-square velocity with frequency, whilst varying mass and/or stiffness of the coupled system, is presented.

• 한국응용과학기술학회지
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• 제26권4호
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• pp.457-466
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• 2009

This study is manufacturing method and analysis of feasibility about collagen peptide from fish scale. This is processed by enzyme hydrolysis, isolating and refining etc. The results of analysis of nutritional composition showed protein content of collagen peptide. In the analysis of constitutive amino acids, the ratio of contents of hydroxyproline and glycine, the characteristics of collagen peptides appeared similar and the contents of glutamic acid and aspartic acid which are involved in protein metabolism. As a result of measurement of total polyphenol content and total flavonoid, it showed that collagen peptide had more contents generally, and the effect of bioactivity of pig-skin collagen peptide appeared higher although different kinds of scale collagen peptide showed a little DPPH radical scavenging ability, total antioxidant capacity by ABTS, ACE inhibitory.

• 한국생산제조학회지
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• 제22권2호
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• pp.287-291
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• 2013

Fault Diagnosis is a process of detecting and isolating faults in a system. On demanding for safety and high reliability systems make it important for some reasons such as economical and environmental incentives. Especially embedded technology and IT technology combined with precise sensing techniques has been doing well developed and applied to fault diagnosis and prognosis in industrial systems like as automotive, ship, heavy industry and aerospace as well. This paper, as an empirical application of diesel engine, presents a method how to get raw data from physical systems, what to consider for successful implementation and which theoretic mathematical models should be applied. In a sense of system level Adaptive Filtering (we call Modified Kalman Filter) and a unit of part level Hidden Markov Process was developed and applied.

• Molecules and Cells
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• 제21권3호
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• pp.376-380
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• 2006

Gene expression is regulated in large part at the level of transcription under the control of sequence-specific transcriptional regulatory proteins. Therefore, the ability to affect gene expression at will using sequencespecific artificial transcription factors would provide researchers with a powerful tool for biotechnology research and drug discovery. Previously, we isolated 56 novel sequence-specific DNA-binding domains from the human genome by in vivo selection. We hypothesized that these domains might be more useful for regulating gene expression in higher eukaryotic cells than those selected in vitro using phage display. However, an unpredictable factor, termed the "context effect", is associated with the construction of novel zinc finger transcription factors--- DNA-binding proteins that bind specifically to 9-base pair target sequences. In this study, we directly selected active artificial zinc finger proteins from a zinc finger protein library. Direct in vivo selection of constituents of a zinc finger protein library may be an efficient method for isolating multi-finger DNA binding proteins while avoiding the context effect.

• Mycobiology
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• 제34권4호
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• pp.180-184
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• 2006

The mycobiota of 160 hair and nail samples collected from 4 different governorates in upper Egypt were estimated using soil plate method for isolating keratinophilic and dermatophytic fungi. Twenty-three fungi were recorded on both hair and nail samples collected from the four governorates. Highest fungal diversity (20) was collected from Red Sea samples followed by Qena (18) and Aswan (17) while lowest fungal diversity was recorded from Sohage samples. The common genera were Aphanoascus, Aspergillus, Penicillium, Paecilomyces and Chrysosporium. The most prevalent species belonging to these genera were: A. fulvescens, Aphanoascus sp. A. flavus link, A. flavus var. columnaris, P. chrysogenium. P. lilacinus and C. sulfureum. True dermatophytes such as Nannizzia fulva appeared in $20{\sim}30%$ of the male samples.

• 약학회지
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• 제45권2호
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• pp.169-179
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• 2001

In previous study, Gyrophora esculenta showed significant inhibitory effect on $\alpha$-glucosidases in vitro and blood glucose elevation in vivo. In the isolating process of active substance, orcinol was separated from Gyrophora esculenta. Orcinol is known to be toxic, therefore, in this study, it was analysed by the TLC densitometry method for quantitative determination from Gyrophora esculenta. The average amount of orcinol of Gyrophora esculenta was 0.2%. For the purpose of removing orcinol, the water extract of Gyrophora esculenta was sequentially fractionated by organic solvents, and the acute toxicity of each fraction was assessed in mice. Among them, the LD50 of butanol fraction was 1.19 g/kg(p.o.) and the weight increase of mice in that group was somewhat retarded.

• 한국군사과학기술학회지
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• 제3권2호
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• pp.140-148
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• 2000

This paper presents a fault detection and isolation(FDI) method based on Generalized Likelihood Ratio(GLR) test for the tightly coupled SDINS/CPS system. The GLR test is known to have the capability of detecting an assumed change while estimating its occurrence time and magnitude, and isolating the changing part. Once a fault is detected even if we don't know if the fault occurrs at either INS or GPS, multi-hypothesized GLR scheme performs the fault isolation between INS and GPS, and find which satellite malfunctions. However, in the INS faulty case, it turned out to fail to accomodate the fault isolation between accelerometer and gyroscope due to the coupling effects and a poor observability of the system. Hence, to isolate the INS fault, it needs to change the attitude of the vehicle resulting in enhancing the degree of observability.

• Journal of Positioning, Navigation, and Timing
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• 제7권4호
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• pp.227-233
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• 2018

The purpose of satellite navigation meaconing jamming is to make the target GPS receiver calculate false navigation by meaconing the received satellite signals. At this time, since the received and transmitted signals have the same frequency, the back-lobe reduction level of antenna should be -160 dB when the Effective Radiated Power (ERP) is 1 Watt (30 dBm). Therefore, meaconing jamming is impossible by merely reducing the back-lobe level of antenna when the transmitter and receiver are in proximity to each other. In general, the transmitter and receiver are isolated by the time division method to eliminate such transmission/reception interference. This paper studied the optimal switching timing between transmitting and receiving when isolating the time division transmission and reception for GPS meaconing jamming.