• Journal of Biosystems Engineering
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• v.29 no.5 s.106
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• pp.385-394
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• 2004

The objectives of this study were to investigate the effectiveness of rubber as an engine mount of walking-type cultivators and to determine its optimal spring constant and damping coefficient using a dynamic simulation of the engine mount system. Four different types of rubber mounts were tested to determine their spring constants and damping coefficients, and the best type was selected for the isolation of the engine vibrations transmitted to the handle. The total vibration levels transmitted to the handle when the rubber mounts weren't installed were 17.52 $m/s^2$. The total vibration levels transmitted to the handle when the rubber mounts were installed were 10.69 $m/s^2$ for Stripe 1, 11.33$m/s^2$ for Stripe 2, 10.92$m/s^2$ for Stripe 3 and 14.19$m/s^2$ for Hive, respectively, resulting in an average of $30\%$ reduction when compared with that without the engine mount. A dynamic model of the cultivator's engine-mount system and its simulation program were developed and verified. A method was proposed to determine the optimal spring constant and damping coefficient of the engine-mount system. It was found from the simulation that a spring constant of 4,100 kN/m and the largest damping coefficient were the most effective for the vibration isolation.

• Microbiology and Biotechnology Letters
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• v.31 no.4
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• pp.342-347
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• 2003

Clumping of cells is one of the major obstacles to culture wild myxobacterial strains in liquid media. In an effort to solve this problem, we tried a method isolating spontaneous mutants that grow dispersed in liquid media from a wild myxobacterial strain. Myxococcus stipitatus KYC1001, a newly isolated strain from Gyearyongsan National Park in Korea, clumps and sticks to the surface of culture vessels as other wild myxobacteria behave in liquid media. Taking an advantage of the characteristics that dispersed mutant cells would grow dispersed while most other wild type cells would clump and stick to the surface of culture vessels, spontaneous dispersed mutants were enriched by repeated subculturing of culture supernatant. A resultant mutant, KYC2001, did not form any clumps nor stick to the surface of culture flasks, but grew completely dispersed in liquid. Meanwhile, three other spontaneous mutants, KYC2002, KYC2003, and KYC2004, shelved partially dispersed phenotype. A major portion of the cells grew dispersed in liquid but they still formed some clumps.

• Journal of the Korean Society for Railway
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• v.14 no.3
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• pp.234-239
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• 2011

Installing the temporary bridge after excavating the railway requires installing movable cross beam, but as it doesn't requires isolating the catenary or cutting the rail, it's applicable to double-track with frequent operation. In this study, a displacement meter was placed on temporary bridge to monitor the displacement pattern in curve section (R400) completed using temporary bridge method, and wheel load, lateral pressure and derailment coefficient were measured to evaluate the load imposed on track and the stability in curve section (R400) for quantitative evaluation of training running safety. As a result of the measurement, when trains passing over a temporary bridge, the maximum value of Wheel load and Lateral Force is analyzed as the 51% and 81% of standard level according to foreign country's performance tests, There is no trouble with stability analysis in Wheel load and Lateral Force occurring. Additionally, Wheel load and Lateral Force considered as the safety standard are tested 49% of limiting value regardless of trains, which the norm value quite well, there is no problem with train running.

• Korean Journal of Microbiology
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• v.48 no.4
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• pp.229-239
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• 2012

Cronobacter spp. (formerly Enterobacter sakazakii), a Gram-negative bacillus, is a rare cause of meningitis and central nervous system infections. In England, the first case infected by this organism occurred in 1958. By July 2008, approximately 120 documented cases of Cronobacter spp. infection and at least 27 deaths have been identified from all around the world in the published literature and in reports submitted by public health sectors. In 2007, it was proposed by European organizations that the original taxonomy of E. sakazakii would be revised, to consist of five new species moved to a new genus, and identified as "Cronobacter". E. sakazakii has thus now been reclassified as 6 separate species in the new genus, Cronobacter, gen. nov., within the Enterobacteriaceae family. The new species are presently Cronobacter sakazakii, C. turicensis, C. malonaticus, C. muytjensii, and C. dublinensis; the sixth species is identified simply as genomospecies I, as currently including only two representative strains. The objectives of this review are to provide insight on (1) the classification and taxonomy of Cronobacter spp., (2) its clinical etiology and pathogenicity, (3) prequency of Cronobacter spp. in different categories of ready-to-eat food other than infant formula, (4) methods for detecting, isolating and typing Cronobacter spp., and (5) recent research trends for detecting Cronobacter spp.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2013.04a
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• pp.390-394
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• 2013

Observation satellites carrying high precision optical payload require extremely stringent pointing stability that may be violated in the presence of the disturbances corning from reaction wheels, cryocoolers or other actuating components onboard the satellite. The most common method to protect the sensitive payloads from external disturbances is implementation of vibration isolator. In this paper development of a single axis active vibration isolator using electromagnet and its performance in isolating micro-vibration is presented. The main components of the developed isolator are membrane structure providing the isolator with the required stiffness and an electromagnet for active control. The performance test results show that additional damping can be achieved by active control without degrading isolation performance in high frequency region and that the developed isolator can effectively isolate micro-vibration.

• Molecules and Cells
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• v.39 no.10
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• pp.768-775
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• 2016

The Arabidopsis female gametophyte contains seven cells with eight haploid nuclei buried within layers of sporophytic tissue. Following double fertilization, the egg and central cells of the gametophyte develop into the embryo and endosperm of the seed, respectively. The epigenetic status of the central cell has long presented an enigma due both to its inaccessibility, and the fascinating epigenome of the endosperm, thought to have been inherited from the central cell following activity of the DEMETER demethylase enzyme, prior to fertilization. Here, we present for the first time, a method to isolate pure populations of Arabidopsis central cell nuclei. Utilizing a protocol designed to isolate leaf mesophyll protoplasts, we systematically optimized each step in order to efficiently separate central cells from the female gametophyte. We use initial manual pistil dissection followed by the derivation of central cell protoplasts, during which process the central cell emerges from the micropylar pole of the embryo sac. Then, we use a modified version of the Isolation of Nuclei TAgged in specific Cell Types (INTACT) protocol to purify central cell nuclei, resulting in a purity of 75-90% and a yield sufficient to undertake downstream molecular analyses. We find that the process is highly dependent on the health of the original plant tissue used, and the efficiency of protoplasting solution infiltration into the gametophyte. By isolating pure central cell populations, we have enabled elucidation of the physiology of this rare cell type, which in the future will provide novel insights into Arabidopsis reproduction.

• Journal of the Mineralogical Society of Korea
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• v.18 no.2
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• pp.83-92
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• 2005

Dissolution rate of minerals may differ from climates configuration, but weathering rate of feldspars is generally proved to be relatively higher The result of geochemical reaction modeling indicates the acid water of pH 4.5 excluding any other variables, was 2.3 times higher than that in ordinary rain of pH 5.7. This result proved that pH is very important factor in preservation of granite cultural properties. To prevent the weathering of stone cultural properties, weathering characteristics of stones should be studied first and constitution of dry environments, using water repellent or oil coating, isolating water which cause chemical weathering reaction like hydration and oxidization should be considered. Considering the long-term reactions between granite and rain, selection of materials, which can bring neutralization and non-oxidization conditions, are very important in using cleaning agents and biological controls.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.162-163
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• 1999

The goal of this research was (1) to describe the conditions and parameters required for the cell cycle synchronization and the accumulation of large number of metaphase cells in maize and other cereal root tips, (2) to isolate intact metaphase chromosomes from root tips suitable for characterization by flow cytometry, and (3) to construct chromosome-specific libraries from maize. Plant metaphase chromosomes have been successfully synchronized and isolated from many cereal root-tips. DNA synthesis inhibitor (hydroxyurea) was used to synchronize cell cycle, follwed by treatement with trifluralin to accumulate metaphase chromosomes. Maize flow karyotypes show substantial variation among inbred lines. thish variation should be sueful in isolating individual chromosome types. In addition, flow cytometry is a useful method to measure DNA content of individual chromosomes in a genotyps, and to detect chromosomal variations. Individual chromosome peaks have been sorted from the maize hybrid B73/Mol7. Libraries were generated form the DOP-PCR amplification product from each peak. To date, we have analyzed clones from a library constructed from the maize chromosome 1 peak. Hybridization of labeled genomic DNA to clone inserts indicated that $24\%,\;18\%,\;and\;58\%$ of the clones were highly repetitive, medium repetitive, and low copy, respectively. Fifty percent of putative low cpoy clones showed single bands on inbred screening, blots, and the remaining $50\%$ were low copy repeats. Single copy clones showing polymorphism will be mapped using recombinant inbred mapping populations. Repetitive clones are being characterized by Southern blot analysis, and will be screened by in situ hybridization for their potential utility as chromosome specific markers.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.4
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• pp.121-126
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• 2010

The automotive weather strip has functions of isolating of water, dust, noise and vibration from outside. To achieve good sealing performance, weather strip should be designed to have the high contact force and wide contact area. However, these design causes excessive permanent deformation of weather strip. The causes of permanent deformation is generally explained to be the chemical material detrioration and physical variation and cyclic loading, etc. This paper introduces a numerical method to predict the permanent deformation using the time dependent viscoelastic model which is represented by Prony series in ABAQUS. Uniaxial tension and creep tests were conducted to obtain the material data. And the lab. test for the permanent deformation was accelerated during shorter time, 300 hours. The permanent deformation of weather strip was successfully predicted under the different loading conditions and different section shapes using the suggested numerical process.

• Journal of Korean Academy of Nursing
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• v.28 no.1
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• pp.104-116
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• 1998

The purpose of this study was to analyze the trends of family nursing research in Korea. The subjects were 62 articles, 132 master theses and 20 doctoral theses published during the period between 1970-1997.7. The results are as follows. 1. The types of research that were analyzed were factor isolating. 34.1%, factor relating, 54.2%, situation relating. 6.1% and situation producing, 3.7%. 2. Research designs were experimental studies, 6. 1%, non-experimental studies, 89.7% and qualitative research, 4.2%. Survey research was the method used in 82.7% of the papers. 3. On the research subjects, 94 papers dealt with the family member of a patient or a single person, 59 papers dealt with a patient and 7 papers dealt with whole family. 4. On the places of studies, 64.0% of the studies were done in hospital rooms, 12.9% in the communities and community facilities. 12.0% in schools, 10.7% in the home, and 0.4% an occupational setting. 5. The most frequently used family concept in the title of the articles was "family support"(59 papers) followed by family function and family burden. 6. The most frequently used family assessment tool was Family Environment Scale(FES) developed by Moos & Insel. 7, According to family nursing domain described by Murphy' and Meister'study, the subdomain, relationship of the family and disease was found 83.2%. 8. Four papers built conceptual frameworks based on various theories of researchers. Eleven papers applied family theories and five papers applied nursing theories. Based on the above findings the following suggestions are made. 1. The various domains of family nursing research should be used including in the relationship of family and health, health policy and family. transcultural family research, and theoretical approach to family. 2. Qualitative research should be used for family nursing research. 3. Family assessment tools should be developed for the Korean family.