• 대한의생명과학회지
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• 제11권3호
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• pp.301-305
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• 2005

MR spectroscopy of intracellularly located $^{133}Cs$ has been used to monitor the uptake of Gd-EOB-DTPA by the isolated rat liver. As shown by ${31}P$ spectroscopy, accumulation of $^{133}Cs$ ions in hepatocytes does not produce detectable effects on the metabolism. The hepatic internalization of Gd-EOB-DTPA was followed by the paramagnetic relaxation enhancement of the intracellular $^{133}Cs$ ions, and confirmed by parallel quantitations of Gd and Cs run by inductively coupled plasma analysis of liver samples and aliquots of perfusate. Two peaks are observed at -22.0 and -23.5 ppm, with respect to the line of the external reference arbitarily set to 0 ppm. Upon rinsing of the extracellular compartment with regular K-H free of CsCl, the high-field resonance disappears within 20min. The intracellular concentration was confirmed by ICP, which gives a $Cs^+$ content of $22.0\pm3.5mM$. The relaxation data significantly underestimate the Gd content, suggesting a potential compartmentation of $Cs^+$ and the contrast agent.

• 대한수의학회지
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• 제39권4호
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• pp.806-810
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• 1999

A multiplex-polymerase chain reaction (PCR) assay was used to detect staphylococcal enterotoxin production by 12 strains of Staphylococcus aureus isolated from clinical specimens. To evaluate the efficacy and/or sensitivity of this method, the results were compared to those obtained with the reversed passive latex agglutination kit (SET-RPLA, Denka Seiken, Japan). Of 10 strains positive by PCR were positive by RPLA but two strains, representing high sensitivity of the former method. Enterotoxin B was the most prevalent by the two methods. The kappa index between the two methods was 0.826, indicating a higher agreement and fully reliable for use. These results would suggest that sensitive, inexpensive, and relatively rapid multiplex-PCR technique may be an effective means for the detection of staphylococcal enterotoxin genes as an alternative to traditional methods such as kits or immunological methods, which depend upon the amount of enterotoxin produced.

• 미생물학회지
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• 제29권3호
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• pp.199-202
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• 1991

The O serotypes of uropathogenic Escherichia coli isolated in Korea were studied using a complete set of rabbit O antisera raised with reference O antigen type strains of E. coli. The distribution of "O" serotypes found in this survey was grossly similar with the prevalence of "O" types observed in other parts of the world, and some differences were also noted. A total of 31 "O" serotypes were identified and the most frequent serotype associated with urinary tract infections was O75(11.5%), which was followed by O6(7.4%), O10 and O40(5.7%, respectively).0 and O40(5.7%, respectively).

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.130-130
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• 2000

This paper presents a robust fault diagnosis and fault tolerant control lot the actuator and sensor faults in the closed-loop systems affected by unknown inputs or disturbances. The fault diagnostic scheme is based on the residual set generation by using robust Parity space approach. Residual set is evaluated through the threshold test and then fault is isolated according to the decision logic table. Once the fault diagnosis module indicates which actuator or sensor is faulty, the fault magnitude is estimated by using the disturbance-decoupled optimal state estimation and a new additive control law is added to the nominal one to override the fault effect on the system. Simulation results show that the method has definite fault diagnosis and fault tolerant control ability against actuator and sensor faults.

• 대한교통학회지
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• 제14권2호
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• pp.45-58
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• 1996

In a large signal intersection, it is the most important to set phase sequences and phase intervals of traffic signal in order to improve the efficiency of the capacity as well as safety. These setting allows to select the best sequence of signal phase among several alternatives, and thus to rearrange the starting and ending points of the individual phase using an effective interphase periods (EIP). The EIP is a gap between previous and current traffic movements at a potential collision point in an intersection. Each of traffic movements has an equality for safety and efficiency at the balanced condition of EIP. This paper presents how to set optimally the phase sequences and intervals of traffic signal in an intersection using phase based approach. And in the second part, we applied the theory developed in the first part. In particular, a numerical example of phase base signal setting is presented using a matrix computation method in order to select the best sequence among several alternatives, and thus to rearrange the starting and ending points of the individual phase using the EIP. This method also allows to apply to optimum signal setting even in five-lag or staggered-type intersection.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 하계종합학술대회 논문집(4)
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• pp.347-350
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• 2002

This paper describes eye detection algorithm using edge information and Support Vector Machine (SVM). We adopt an edge detection and labelling algorithm to detect isolated components. Detected candidate eye pairs finally verified by SVM using Radial Basis Function (RBF) kernel. A detection rate over the test set has been achieved more than 90%, and compared with template matching method. this proposed method significantly reduced FAR.

• 한국컴퓨터정보학회논문지
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• 제22권12호
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• pp.117-123
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• 2017

The personalized search algorithm is a search system that analyzes the user's IP, cookies, log data, and search history to recommend the desired information. As a result, users are isolated in the information frame recommended by the algorithm. This is called 'Filter bubble' phenomenon. Most of the personalized data can be deleted or changed by the user, but data stored in the service provider's server is difficult to access. This study suggests a way to neutralize personalization by keeping on sending random query words. This is to confuse the data accumulated in the server while performing search activities with words that are not related to the user. We have analyzed the rank change of the URL while conducting the search activity with 500 random query words once using the personalized account as the experimental group. To prove the effect, we set up a new account and set it as a control. We then searched the same set of queries with these two accounts, stored the URL data, and scored the rank variation. The URLs ranked on the upper page are weighted more than the lower-ranked URLs. At the beginning of the experiment, the difference between the scores of the two accounts was insignificant. As experiments continue, the number of random query words accumulated in the server increases and results show meaningful difference.

• 전력전자학회논문지
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• 제13권2호
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• pp.128-134
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• 2008

독립형 전원시스템에서 엔진을 이용한 발전시스템은 지금까지 에너지의 효율적인 측면보다는 설치 단가 및 신뢰성이 중요한 이슈가 되어왔으나, 최근 들어 신뢰성과 함께 에너지의 효율적 사용, 대기 환경 개선 및 발전 전력 품질 등이 점차 중요시 되어가고 있다. 가변속 엔진 발전 시스템의 경우 출력 전력에 대응하여 엔진 속도를 가변함으로써, 경부하시 획기적인 효율 개선이 가능하다. 또한 부하 전압, 주파수는 출력 인버터에 의해 제어되므로, 그 응답 속도가 빨라 고 품질의 전력을 전원에 공급할 수 있다. 특히 급격한 부하 증가 시 엔진 출력이 늘어나는 시간동안 부족한 전력을 슈퍼캐패시터로부터 공급받음으로써, 부하 급증 시에도 안정된 전력 공급이 가능하다. 본 논문에서는 소형 디젤 엔진과 슈퍼캐패시터를 이용한 가변속 엔진 발전 시스템의 개념을 설명하고, 가변속 운전의 필요성, 고정 운전속 대비 연비 절감 및 3상 4선식 인버터를 사용한 고품질의 전력 공급 등의 타당성을 모의 실험과 실험을 통하여 검증하도록 한다.

• 한국해양바이오학회지
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• 제1권3호
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• pp.163-169
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• 2006

본 연구에서는 비장 내의 비대해진 세포 존재와 조직 괴사라는 병리조직학적 관찰에 의하여 우리 나라의 pearl gourami (Trichogaster leeri)에서 iridovirus에 의한 자연 감염이 나타남을 확인하였다. 이러한 iridovirus 감염을 더욱 정확하게 진단하기 위해서 iridovirus 감염 진단에 주로 사용되는 MCP와 ATPase gene 부위에서 2 primer sets를 제작하여 PCR을 실시한 결과, PCR 생성물은 기대한 size와 부합하게 나타났고, MCP gene 부위의 염기서열은 reference strain인 ISKNV와 매우 높은 유사성 (99.6%)을 보였다. 공격 실험을 통하여 pearl gourami에서 분리된 iridovirus의 병원성을 확인하였고, 무분별한 관상어 관리에 의하여 관상어로부터 양식어류에로의 질병 전이가 나타남으로서 일어날 수 있는 국내 양식 산업에 대한 위험성을 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제22권8호
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• pp.1141-1147
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• 2012

Polyhydroxyalkanoate (PHA) is a class of biodegradable plastics that have great potential applications in the near future. In this study, the micro-biodiversity and productivity of PHA-accumulating bacteria in activated sludge from a domestic wastewater treatment plant were investigated. A previously reported primer set and a self-designed primer set (phaCF1BO/phaCR2BO) were both used to amplify the PHA synthase (phaC) gene of isolated colonies. The new primers demonstrated higher sensitivity for phaC, and combining the PCR results of the two primer sets was able to widen the range of detected genera and raise the sensitivity to nearly 90%. Results showed that 85.3% of the identified bacteria were Gram-negative, with Ralstonia as the dominant genus, and 14.7% were Gram-positive. In addition, Zoogloea and Rhizobium contained the highest amounts of intracellular PHA. It is apparent that glucose was a better carbon source than pentone or tryptone for promoting PHA production in Micrococcus. Two different classes, class I and class II, of phaC were detected from alphaproteobacteria, betaproteobacteria, and gammaproteobacteria, indicating the wide diversity of PHA-accumulating bacteria in this particular sampling site. Simultaneous wastewater treatment and PHA production is promising by adopting the high PHA-accumulating bacteria isolated from activated sludge.