• 제목/요약/키워드: isolated set

검색결과 278건 처리시간 0.033초

Sequence Analysis of the Coat Protein Gene of a Korean Isolate of Iris Severe Mosaic Potyvirus from Iris Plant

  • Park, Won-Mok;Lee, Sang-Seon;Park, Sun-Hee;Ju;Ryu, Ki-Hyun
    • The Plant Pathology Journal
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    • 제16권1호
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    • pp.36-42
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    • 2000
  • The coat protein gene of iris severe mosaic potyvirus, which was isolated in Korea, ISMV-K, from iris plant was cloned and its nucleotide sequence was determined. The coat protein of the virus contained 252 amino acid residues, including five potential N-glyxosylation site motifs. The coat protein of ISMV-K has 99.1% and 98.4% sequence identities with those of the Netherlands isolate of ISMV (ISMV-Ne) form crocus for the nucleotide and amino acids, respectively. The coat protein of ISMV-K has 50.4% to 60.3% nucleotide sequence identities and 47.3% to 55.7% amino acid identities with those of other 21 potyviruses, indicating ISMV to be a distinct species of the genus. The coat protein of ISMV-K was closely related with bean yellow mosaic virus and clover yellow vein virus in the phylogenetic tree analysis among the potyviruses analyzed. ISMV was easily and reliably detected from virus-infected iris leaves by RT-PCR with a set of the virus-specific primers.

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Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches

  • Li, Weilan;Lee, Seung-Yeol;Back, Chang-Gi;Ten, Leonid N.;Jung, Hee-Young
    • The Plant Pathology Journal
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    • 제35권6호
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    • pp.635-643
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    • 2019
  • To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA of X. arboricola pv. pruni strain but not from 12 other Xanthomonas species strains and 6 strains of other genera. The LAMP conditions were checked with the healthy leaves of 31 peach varieties, and no reaction was detected using either the peach leaves or the peach DNA as a template. Furthermore, the high diagnostic accuracy of the LAMP method was confirmed with 13 X. arboricola pv. pruni strains isolated from various regions in Korea, with all samples exhibiting a positive reaction in LAMP assays. In particular, the LAMP method successfully detected the pathogen in diseased peach leaves and fruit in the field, and the LAMP conditions were proven to be a reliable diagnostic method for the specific detection and identification of X. arboricola pv. pruni in peach orchards.

Impact of Lyman alpha pressure on metal-poor dwarf galaxies

  • Kimm, Taysun;Haehnelt, Martin;Blaizot, Jeremy;Katz, Harley;Michel-Dansac, Leo;Garel, Thibault;Rosdahl, Joakim;Teyssier, Romain
    • 천문학회보
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    • 제43권1호
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    • pp.36.1-36.1
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    • 2018
  • Understanding the origin of strong galactic outflows and the suppression of star formation in dwarf galaxies is a key problem in galaxy formation. Using a set of radiation-hydrodynamic simulations of an isolated dwarf galaxy, we show that the momentum transferred from resonantly scattered Lyman-alpha(LyA) photons can suppress star formation by a factor of two in metal-poor galaxies by regulating the dynamics of star-forming clouds before the onset of supernova explosions (SNe). This is possible because each LyA photon resonantly scatters and imparts ~10-300 times greater momentum than in the single scattering limit. Consequently, the number of star clusters predicted in the simulations is reduced by a factor of ~5, compared to the model without the early feedback. More importantly, we find that galactic outflows become weaker in the presence of strong LyA radiation feedback, as star formation and associated SNe become less bursty. We also examine a model in which radiation field is arbitrarily enhanced by a factor of up to 10, and reach the same conclusion. The typical mass-loading factors in our metal-poor dwarf system are estimated to be ~5-10 near the mid-plane, while it is reduced to ~1 at larger radii.

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Displacement aging component-based stability analysis for the concrete dam

  • Huang, Xiaofei;Zheng, Dongjian;Yang, Meng;Gu, Hao;Su, Huaizhi;Cui, Xinbo;Cao, Wenhan
    • Geomechanics and Engineering
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    • 제14권3호
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    • pp.241-246
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    • 2018
  • The displacement monitoring data series reconstruction method was developed under equal water level effects based on displacement monitoring data of concrete dams. A dam displacement variation equation was set up under the action of temperature and aging factors by optimized analysis techniques and then the dam displacement hydraulic pressure components can be separated. Through the dynamic adjustment of temperature and aging effect factors, the aging component isolation method of dam displacement was developed. Utilizing the isolated dam displacement aging components, the dam stability model was established. Then, the dam stability criterion was put forward based on convergence and divergence of dam displacement aging components and catastrophe theory. The validity of the proposed method was finally verified combined with the case study.

꼬임식 테이프가 설치된 사각 덕트에서 스월유동이 미치는 열전달과 마찰계수 (Effects of Swirl Flow Generated by Twisted Tape on Heat Transfer and Friction Factor in a Square Duct)

  • 강호근;아리바시아크리시나 부트라;안수환
    • 한국전산유체공학회:학술대회논문집
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    • 한국전산유체공학회 2008년도 춘계학술대회논문집
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    • pp.117-120
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    • 2008
  • Numerical simulations and experiment of a hydrodynamic and thermally developed turbulent flow through square ducts (3.0 ${\times}$ 3.0 cm) with twisted tape inserts and with twisted tape inserts plus interrupted ribs are conducted to investigate regionally averaged heat transfer and friction factors. Turbulent swirl flows having Reynolds numbers ranging from 8,900 to 29,000, a rib height-to-channel hydraulic diameter(e/D$_h$) of 0.067, and a length-to-hydraulic diameter(L/D$_h$) of 30, are considered. The square ribs are arranged to follow the trace of the twisted tape and along the flow direction defined as axial interrupted ribs. The twisted tape has 0.1 mm thick carbon steel sheet with diameter of 2.8 cm, length of 90 cm, and 2.5 turns. Each wall is composed of isolated aluminum sections, and two cases of surface heating are set. The results show that uneven surface heating enhances the heat transfer coefficient over uniform heating conditions, and square ducts with twisted tape inserts plus interrupted ribs produces the best overall transfer performance.

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Direct Single-stage Power Converter with Power Factor Improvement for Switched Mode Power Supply

  • Kalpana, R.;Singh, Bhim;Bhuvaneswari, G.
    • Journal of Electrical Engineering and Technology
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    • 제5권3호
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    • pp.468-476
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    • 2010
  • This paper presents a direct single-stage power converter using single-phase isolated full-bridge converter modules, with inherent power factor correction (PFC) for a 12 kW switched mode power supply (SMPS). The advantages of the proposed converter are its simple control strategy, reduction in number of conversion stage, low input line current harmonics, and improvement in power factor. Analysis of the single-stage converter is carried out in continuous conduction mode of operation. Steady-state analysis of the proposed converter is conducted to obtain converter parameters. A systematic design procedure is also presented for a 12k W converter with a design example. The effect of load variation on SMPS is also studied in order to demonstrate the effectiveness of the proposed converter for the complete range of load conditions. A set of power quality indices on input ac mains for an SMPS fed from a single-stage converter is also presented for easy comparison of their performance.

Analysis of Fungicide Sensitivity and Genetic Diversity among Colletotrichum Species in Sweet Persimmon

  • Gang, Geun-Hye;Cho, Hyun Ji;Kim, Hye Sun;Kwack, Yong-Bum;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • 제31권2호
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    • pp.115-122
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    • 2015
  • Anthracnose, caused by Colletotrichum gloeosporioides (C. gloeosporioides; Teleomorph: Glomerella cingulata), is the most destructive disease that affects sweet persimmon production worldwide. However, the biology, ecology, and genetic variations of C. gloeosporioides remain largely unknown. Therefore, in this study, the development of fungicide resistance and genetic diversity among an anthracnose pathogen population with different geographical origins and the exposure of this population to different cultivation strategies were investigated. A total of 150 pathogen isolates were tested in fungicide sensitivity assays. Five of the tested fungicides suppressed mycelial pathogen growth effectively. However, there were significant differences in the sensitivities exhibited by the pathogen isolates examined. Interestingly, the isolates obtained from practical management orchards versus organic cultivation orchards showed no differences in sensitivity to the same fungicide. PCR-restriction fragment length polymorphism (RFLP) analyses were performed to detect internal transcribed spacer regions and the ${\beta}$-tubulin and glutamine synthetase genes of the pathogens examined. Both the glutamine synthetase and ${\beta}$-tubulin genes contained a complex set of polymorphisms. Based on these results, the pathogens isolated from organic cultivation orchards were found to have more diversity than the isolates obtained from the practical management orchards.

Occurrence of Leaf Blight on Cosmos Caused by Alternaria cosmosa in Korea

  • Deng, Jian Xin;Lee, Ji Hye;Paul, Narayan Chandra;Cho, Hye Sun;Lee, Hyang Burm;Yu, Seung Hun
    • The Plant Pathology Journal
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    • 제31권1호
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    • pp.78-82
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    • 2015
  • In 2011, a leaf blight disease was observed on cosmos (Cosmos bipinnatus) leaves in Nonsan, Korea. The causal pathogen was isolated and identified based on morphological and molecular approaches. Morphological characteristics of the pathogen matched well with the Alternaria cosmosa and also easily distinguishable from Alternaria zinniae reported from cosmos seeds by producing branched beak. Phylogenetically, the pathogen could not be distinguished from A. passiflorae based on the sequence analysis of a combined data set of Alt a1 and gpd genes. However, A. passiflorae was distinguished from the present species by having conidiophores with 4 to 5 conidiogenous loci. The results indicate that the present Alternaria species is A. cosmosa. Pathogenicity tests revealed that the isolate was pathogenic to the leaves of Cosmos bipinnatus. This is the first report of Alternaria blight disease caused by A. cosmosa on cosmos in Korea.

신호교차로의 차로 배정과 신호시간 최적화 모형에 관한 연구 (A Study on Optimization of Lane-Use and Traffic Signal Timing at a Signalized Intersection)

  • 김주현;신언교
    • 한국도로학회논문집
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    • 제17권5호
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    • pp.93-103
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    • 2015
  • PURPOSES : The purpose of this study is to present a linear programing optimization model for the design of lane-based lane-uses and signal timings for an isolated intersection. METHODS: For the optimization model, a set of constraints for lane-uses and signal settings are identified to ensure feasibility and safety of traffic flow. Three types of objective functions are introduced for optimizing lane-uses and signal operation, including 1) flow ratio minimization of a dual-ring signal control system, 2) cycle length minimization, and 3) capacity maximization. RESULTS : The three types of model were evaluated in terms of minimizing delay time. From the experimental results, the flow ratio minimization model proved to be more effective in reducing delay time than cycle length minimization and capacity maximization models and provided reasonable cycle lengths located between those of other two models. CONCLUSIONS : It was concluded that the flow ratio minimization objective function is the proper one to implement for lane-uses and signal settings optimization to reduce delay time for signalized intersections.

Modelling the shapes of the largest gravitationally bound objects

  • Rossi, Graziano;Sheth, Ravi K.;Tormen, Giuseppe
    • 천문학회보
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    • 제36권2호
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    • pp.53.2-53.2
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    • 2011
  • We combine the physics of the ellipsoidal collapse model with the excursion set theory to study the shapes of dark matter halos. In particular, we develop an analytic approximation to the nonlinear evolution that is more accurate than the Zeldovich approximation; we introduce a planar representation of halo axis ratios, which allows a concise and intuitive description of the dynamics of collapsing regions and allows one to relate the final shape of a halo to its initial shape; we provide simple physical explanations for some empirical fitting formulae obtained from numerical studies. Comparison with simulations is challenging, as there is no agreement about how to define a non-spherical gravitationally bound object. Nevertheless, we find that our model matches the conditional minor-to-intermediate axis ratio distribution rather well, although it disagrees with the numerical results in reproducing the minor-to-major axis ratio distribution. In particular, the mass dependence of the minor-to-major axis distribution appears to be the opposite to what is found in many previous numerical studies, where low-mass halos are preferentially more spherical than high-mass halos. In our model, the high-mass halos are predicted to be more spherical, consistent with results based on a more recent and elaborate halo finding algorithm, and with observations of the mass dependence of the shapes of early-type galaxies. We suggest that some of the disagreement with some previous numerical studies may be alleviated if we consider only isolated halos.

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