• Biomedical Science Letters
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• v.11 no.3
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• pp.301-305
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• 2005

MR spectroscopy of intracellularly located $^{133}Cs$ has been used to monitor the uptake of Gd-EOB-DTPA by the isolated rat liver. As shown by ${31}P$ spectroscopy, accumulation of $^{133}Cs$ ions in hepatocytes does not produce detectable effects on the metabolism. The hepatic internalization of Gd-EOB-DTPA was followed by the paramagnetic relaxation enhancement of the intracellular $^{133}Cs$ ions, and confirmed by parallel quantitations of Gd and Cs run by inductively coupled plasma analysis of liver samples and aliquots of perfusate. Two peaks are observed at -22.0 and -23.5 ppm, with respect to the line of the external reference arbitarily set to 0 ppm. Upon rinsing of the extracellular compartment with regular K-H free of CsCl, the high-field resonance disappears within 20min. The intracellular concentration was confirmed by ICP, which gives a $Cs^+$ content of $22.0\pm3.5mM$. The relaxation data significantly underestimate the Gd content, suggesting a potential compartmentation of $Cs^+$ and the contrast agent.

• Korean Journal of Veterinary Research
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• v.39 no.4
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• pp.806-810
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• 1999

A multiplex-polymerase chain reaction (PCR) assay was used to detect staphylococcal enterotoxin production by 12 strains of Staphylococcus aureus isolated from clinical specimens. To evaluate the efficacy and/or sensitivity of this method, the results were compared to those obtained with the reversed passive latex agglutination kit (SET-RPLA, Denka Seiken, Japan). Of 10 strains positive by PCR were positive by RPLA but two strains, representing high sensitivity of the former method. Enterotoxin B was the most prevalent by the two methods. The kappa index between the two methods was 0.826, indicating a higher agreement and fully reliable for use. These results would suggest that sensitive, inexpensive, and relatively rapid multiplex-PCR technique may be an effective means for the detection of staphylococcal enterotoxin genes as an alternative to traditional methods such as kits or immunological methods, which depend upon the amount of enterotoxin produced.

• Korean Journal of Microbiology
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• v.29 no.3
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• pp.199-202
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• 1991

The O serotypes of uropathogenic Escherichia coli isolated in Korea were studied using a complete set of rabbit O antisera raised with reference O antigen type strains of E. coli. The distribution of "O" serotypes found in this survey was grossly similar with the prevalence of "O" types observed in other parts of the world, and some differences were also noted. A total of 31 "O" serotypes were identified and the most frequent serotype associated with urinary tract infections was O75(11.5%), which was followed by O6(7.4%), O10 and O40(5.7%, respectively).0 and O40(5.7%, respectively).

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.130-130
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• 2000

This paper presents a robust fault diagnosis and fault tolerant control lot the actuator and sensor faults in the closed-loop systems affected by unknown inputs or disturbances. The fault diagnostic scheme is based on the residual set generation by using robust Parity space approach. Residual set is evaluated through the threshold test and then fault is isolated according to the decision logic table. Once the fault diagnosis module indicates which actuator or sensor is faulty, the fault magnitude is estimated by using the disturbance-decoupled optimal state estimation and a new additive control law is added to the nominal one to override the fault effect on the system. Simulation results show that the method has definite fault diagnosis and fault tolerant control ability against actuator and sensor faults.

• Journal of Korean Society of Transportation
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• v.14 no.2
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• pp.45-58
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• 1996

In a large signal intersection, it is the most important to set phase sequences and phase intervals of traffic signal in order to improve the efficiency of the capacity as well as safety. These setting allows to select the best sequence of signal phase among several alternatives, and thus to rearrange the starting and ending points of the individual phase using an effective interphase periods (EIP). The EIP is a gap between previous and current traffic movements at a potential collision point in an intersection. Each of traffic movements has an equality for safety and efficiency at the balanced condition of EIP. This paper presents how to set optimally the phase sequences and intervals of traffic signal in an intersection using phase based approach. And in the second part, we applied the theory developed in the first part. In particular, a numerical example of phase base signal setting is presented using a matrix computation method in order to select the best sequence among several alternatives, and thus to rearrange the starting and ending points of the individual phase using the EIP. This method also allows to apply to optimum signal setting even in five-lag or staggered-type intersection.

• Proceedings of the IEEK Conference
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• 2002.06d
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• pp.347-350
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• 2002

This paper describes eye detection algorithm using edge information and Support Vector Machine (SVM). We adopt an edge detection and labelling algorithm to detect isolated components. Detected candidate eye pairs finally verified by SVM using Radial Basis Function (RBF) kernel. A detection rate over the test set has been achieved more than 90%, and compared with template matching method. this proposed method significantly reduced FAR.

• Journal of the Korea Society of Computer and Information
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• v.22 no.12
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• pp.117-123
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• 2017

The personalized search algorithm is a search system that analyzes the user's IP, cookies, log data, and search history to recommend the desired information. As a result, users are isolated in the information frame recommended by the algorithm. This is called 'Filter bubble' phenomenon. Most of the personalized data can be deleted or changed by the user, but data stored in the service provider's server is difficult to access. This study suggests a way to neutralize personalization by keeping on sending random query words. This is to confuse the data accumulated in the server while performing search activities with words that are not related to the user. We have analyzed the rank change of the URL while conducting the search activity with 500 random query words once using the personalized account as the experimental group. To prove the effect, we set up a new account and set it as a control. We then searched the same set of queries with these two accounts, stored the URL data, and scored the rank variation. The URLs ranked on the upper page are weighted more than the lower-ranked URLs. At the beginning of the experiment, the difference between the scores of the two accounts was insignificant. As experiments continue, the number of random query words accumulated in the server increases and results show meaningful difference.

• The Transactions of the Korean Institute of Power Electronics
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• v.13 no.2
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• pp.128-134
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• 2008

The variable speed engine generator set is interested for an isolated power system due to reduced fuel consumption and less emission. However, because of sluggish dynamic behavior of the internal combustion engine the power quality would be degraded during the sudden load power surge, where the power required by the load is not available by the engine due to the reduced engine speed. An isolated power system based on variable speed engine with a super-capacitor bank can improve dynamic characteristics under such a sudden load change, and power quality, fuel consumption, and pollutants can be improved remarkably. Also, it is verified by the computer simulation that the inverter of 3phase-4leg is compatible to the isolated power system with unbalanced load. In this paper, the feasibility of the system has been verified based on a 26kw commercial diesel engine system.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.3
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• pp.163-169
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• 2006

In the survey of the samples infected by iridovirus, we found the natural outbreak of iridovirus disease in the ornamental fish, pearl gourami (Trichogaster leeri) in Korea. It was characterized by the appearance of enlarged cells and necrosis in the observation of the imprinted spleen cells under iridovirus. To determine the infection of iridovirus more accurately, PCR using 2 different primer sets for MCP and ATPase gene used for the diagnosis of iridovirus infection was done and found the produced fragments matched with the size of expectation. Partially determined nucleotide sequences of the MCP gene of the iridovirus isolated in this study showed very high homology (99.6%) with that of ISKNV as a reference strain. In challenge experiment with the iridovirus isolated from the moribund of pearl gourami, the pathogenicity of the isolated iridovirus was confirmed and suggested the potential of the risk associated the transfer of iridovirus from the ornamental fishes to the marine culturing fishes.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1141-1147
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• 2012

Polyhydroxyalkanoate (PHA) is a class of biodegradable plastics that have great potential applications in the near future. In this study, the micro-biodiversity and productivity of PHA-accumulating bacteria in activated sludge from a domestic wastewater treatment plant were investigated. A previously reported primer set and a self-designed primer set (phaCF1BO/phaCR2BO) were both used to amplify the PHA synthase (phaC) gene of isolated colonies. The new primers demonstrated higher sensitivity for phaC, and combining the PCR results of the two primer sets was able to widen the range of detected genera and raise the sensitivity to nearly 90%. Results showed that 85.3% of the identified bacteria were Gram-negative, with Ralstonia as the dominant genus, and 14.7% were Gram-positive. In addition, Zoogloea and Rhizobium contained the highest amounts of intracellular PHA. It is apparent that glucose was a better carbon source than pentone or tryptone for promoting PHA production in Micrococcus. Two different classes, class I and class II, of phaC were detected from alphaproteobacteria, betaproteobacteria, and gammaproteobacteria, indicating the wide diversity of PHA-accumulating bacteria in this particular sampling site. Simultaneous wastewater treatment and PHA production is promising by adopting the high PHA-accumulating bacteria isolated from activated sludge.