• 한국미생물·생명공학회지
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• 제18권4호
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• pp.390-393
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• 1990

Trichoderma viride의 cellobiohydrolase을 immunoaffinity chromatography를 제조하여 조효소로부터 일단계로 용이하게 분리할 수 있었다. 분리된 cellobiohydrolase는 전기영동과 isoelectrofocusing 상에서 단일단백질로 확인되었고, 분자량이 70,000,pI치는 3.8.인 산성단백질이었다. 면역친화 정제된 cellobiohydrolase는 무정형 섬유소보다는 고결정성 섬유소에 특성이 높았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1986년도 춘계 학술대회지
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• pp.44-45
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• 1986

• 한국잠사곤충학회지
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• 제43권2호
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• pp.67-76
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• 2001

The "crescent-egg" a new spontaneous mutant was detected in a white egg strain k37. Studies were carried out the linkage analysis, to investigate phenotypic characteristics and biochemical analysis of haemolymph and ovarian protein. The mutant, ore was independent from 20 linkage groups P(2), Ze(3),L(4), oc(5), sn8), Ia(9), w-1(10), K(11), ch(13), U(14), bl(15), cts(16), bts(17), mln(18), nb(19), oh(20),Lan(21), or(22), tub(23) and Xan(27). The fertilization, hatchability and larval growth were not different from the those of normal eggs. The content and composition of yolk protein were similared to normal eggs. Scanning electron microscopy revealed the areal specific structure in dorsal region of egg-shell of cre mutant. Analysis of chorion protein by isoelectrofocusing(IEF), was resolved no difference in the composite of the chorion protein. We conclude that the egg mutant ere is expressed only in the egg-shape formation and region specific determination.

• Journal of Plant Biology
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• 제39권3호
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• pp.173-177
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• 1996

A novel calcium binding protein (CaBP) was purified to electrophoretic homogeneity from Dunaliella salina. In the course of purification experiment, this CaBP was identified as a monomer and its molecular weight was about 21 kDand isoelectric point (pI) value was about 4.1 using isoelectrofocusing. This CaBP was able to bind Ca2+ even in the pressence of an excess MgCl2 and KCI both in solution. In the SDS-PAGE, the Ca2+-bound form was slower than the Ca2+-free form in the nondenaturing PAGE. This means that the CaBP undergoes conformational change in the Ca2+-bound condition. Furthermore, UV absorption spectrum and fluorescence intensity of this CaBP was investigated. UV absorption peak was appeared at about 258 nm and decreased somewhat in Ca2+-bound condition. In the measurement of fluorescence, maximum intensity was appeared at 303 nm and decreased in Ca2+-bound state, similarly as UV absorption spectrum. These show distinct changes upon Ca2+-binding, which indicate of structural and/or dynamic changes largely reminiscent of other members of the EF-hand Ca2+-binding protein family.

• Applied Biological Chemistry
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• 제32권2호
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• pp.85-90
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• 1989

다수확계(통일벼) 및 일반계 각각 3가지 품종의 각 출수후 6시기의 쌀종자에서 2% sodium dodecyl sulfate/5% ${\beta}-mercaptoethanol$로 단백질을 추출하여 1차원에서 등전점에 따라 분리한 후 2차원에서 분자량에 따라 분리하는 2차원 전기영동으로 각각의 단백질 지도를 작성하였다. 종합단백질 지도를 작성하였고 pH $5.2{\sim}8.3$, 분자량 $20,000{\sim}100,000$의 범위에서 300개 이상의 spots이 관찰되었다. 품종 및 출수시기에 따라 단백질 지도가 많은 차이를 보였다.

• 약학회지
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• 제35권3호
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• pp.222-230
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• 1991

Polyphenol oxidase (PPO) was purified from an extract of Perillae Folium by ammonium sulfate fractionation and sephadex G-150 gel filtration, which molecular weight estimated 65,000$\pm$1,000 in SDS-gel electrophoresis, and pI value was 4.8. The pH and temperature optima were 6.0 and $30^{\circ}C$ respectively. $K_{m}$ values of the PPO for various phenolics derived from Lineweaver-Burk plots were 4.0$\times$10$^{-4}$, caffeic acid; 4.2$\times$10$^{-3}$M, 4-methylcatechol. The inhibition by 4-nitrocatechoi, potassium cyanide, cysteine, 2-mercaptoethanol was competitive with $K_{i}$ values of 7.6$\times$10$^{-5}$M, 7.2$\times$10$^{-5}$M, 3.6$\times$10$^{-5}$M, 2.2$\times$10$^{-5}$M, respectively. Among the divalent cations, Cu$^{2+}$ ion was strong activator on PPO and Zn$^{2+}$, Ni$^{2+}$ ions were little effect on PPO activity. In comparing the amino acid composition of Perillae Folium PPO with that of wheat isozyme, grape, spinach showed similarity. But the content of glycine phenylalanine was abundant relatively.

• 한국작물학회지
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• 제39권2호
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• pp.121-127
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• 1994

(1-3)-$\beta$-glucanase 동위효소의 발현 양상을 등 전점 전기영동 젤에서 직접 검출 확인할 수 있는 방법을 개발하였다. 개발된 방법은 시판되고 있는 (1-3)-$\beta$-glucanase활성 측정용 염료착색 기질을 이용하였다. 본 방법은 신속, 간편하며 보리 종자에서 발현되는 것으로 알려져 있는 모든 (1-3)-$\beta$glucanase 동위효소를 검출할 수 있을 정도로 민감하고 특이적이었다. 시판되고 있는 Penicillium(1-3)-$\beta$-glucanase에 대한 활성 검출 한계단위는 50 $\mu$U 정도로 추정되었다. 따라서, 본 방법은 특별한 시설이나 연구 인력을 확보하고 있지 않는 연구실에서 식물체의 (1-3)-$\beta$-glucanase발현에 대한 단백질 수준에서의 연구를 수행하는데 유용하게 이용될 수 있을 것으로 생각된다.

• The Plant Pathology Journal
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• 제21권3호
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• pp.275-282
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• 2005

A chitinolytic bacterium, Chromobacterium sp. strain C-61, was found strongly antagonistic to Rhizoctonia solani, a causal agent of damping-off of eggplant. In this study, the biocontrol activity and enzymatic characteristics of strain C-61 were compared with its four Tn5 insertion mutants (C61-A, -B, -C, and -D) that had lower chitinolytic ability. The chitinase activity of a 2-day old culture was about $76\%,\;49\%\;and\;6\%$ level in C61-A, C61-B and in C61-C, respectively, compared with that of strain C-61. The $\beta-N-acetylhexosaminidase$(Nahase) activity was little detected in strain C-61 but increased largely in C-61A, C61-B and C61-C. Activities of chitinase and Nahase appeared to be negatively correlated in these strains. Another mutant, C-61D, produced no detectable extracellular chitinase and Nahase. The in vitro and in vivo biocontrol activities of strain C-61 and its mutants were closely related to their ability to produce chitinase but not Nahase. No significant differences in population densities between strain C-61 and its mutants were observed in soil around eggplant roots. The results of SDS-PAGE and isoelectrofocusing showed that a major chitinase of strain C-61 is 54-kDa with pI of approximately 8.5. This study provides evidence that the biocontrol activity of Chromobacterium sp. strain C-61 against Rhizoctonia solani depends on the ability to produce chitinase with molecular weight of 54-kDa and pI of 8.5.

• Journal of Plant Biology
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• 제27권2호
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• pp.61-72
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• 1984

The concept of natural grouping of plant designated as the "Amentiferous" is no longer given serious credence, and many of the families included in this grouping have been dispersed in diverse order. Because a review of taxonomic treatments of amentiferous taxa reveals diverse classifications, it has become necessary to investigate new characteristics and attempt to determine the significance of these characteristics in terms of amentiferous taxonomy. Protein analyses by isoelectrofocusing(IEF) and rocket immunoelectrophoresis(RIE) have proved to be useful in the delicitation of Quercus taxa using pollen extracts from selected taxa. When Quercus pollen extracts were separated by electrophoresis based on their isoelectric points in a stable pH gradient and substrates for estrase activity were stained, ten bands were revealed between pH 5-14. Within Lepidobalanus grouping, a great diversity in the pollen protein zymograms was observed with some segregation corresponding to the designated taxonomic sections. Two taxa of Cyclobalanopsis produced a zymogram that is somewhat similar to taxa included within the section Prinus of Lepidobalanus, and less similar to taxa within the section Cerris of the same subgenus. Three tested taxa of the Cerris are in the similar zymogram each other, being segregable from the taxa of Prinus. Quantitative and qualitative analyses for serological relationships within and among th Quercus were also employed. To calculate the degree of protein similarity, total rocket heights obtained from RIE provided an index of serological correspondence(SC). It is reconfirmed that the Quercus is distantly separated from the Fagus according to SC. Comparative data from rocket number and SC in the tested taxa of Quercus also indicate that Lepidobalanus is separable from Cyclobalanopsis. Within the Lepidobalanus Q. acutissima and Q. acutissima x variabilis are almost homogeneors and distinguishable from the other tested taxa of same subgenus. Although the number of taxa tested has been limited, the overall serological evidence best reflects the classification proposed by Redher(1940) and Melchior (1964), having the genus Quercus subdivided into three subgunera: Erythrobalanus, Lepidobalanus, and Cyclobalanopsis.alanopsis.

• 한국작물학회지
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• 제29권1호
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• pp.39-45
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• 1984

본 실험은 수도의 유전연구에 전기영동법을 도입함에 있어 기초조사를 하기 위하여 수도 조직내의 몇가지 산소가 부위별로 차이가 있는지 또한 생장 조절제인 GA를 처이함으로 같은 산소의 동위효소 pattern이 변하는지 '아끼바레'와 '유신' 두 품종을 사용 조사하였다. 그 결과는 다음과 같았다. 1. Isoelectrofocusing에 의한 종자, Shoot, Root의 Esterase, Phosphatase, Amylase 동위효소 pattern은 두 품종 모두 조직부위별로 다른 pattern을 보였다. 2. 7％ polyacrglamide slab gel electrophoresis에 의한 Peroxidase 동위효소 pattern도 두 품종 모두 조직부위별로 다른 Pattern을 보였다. 3. GA(10/sup -5/)을 처리했을 경우 Esterase 동위효소 pattern은 '아끼바레'의 root와 '유신'의 shoot, root에서 대조구와 차이가 있었으며 Phosphatase의 동위효소 pattern은 '아끼바레'의 root에서 차이가 있었다. Amylase와 Peroxidase의 동위효소 pattern은 대조구와 GA 처리간에 차이가 없었다.