• 한국강구조학회 논문집
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• 제22권1호
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• pp.1-12
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• 2010

현행 강구조내진설계철학의 근거인 역량설계법(capacity design method)에 의할 때 중심가새골조의 에너지 소산요소인 가새가 인장항복하고 압축좌굴 할 때 보와 기둥은 탄성상태를 유지해야 한다. 중심가새골조의 대표적 형식인 역V형 가새골조의 경우 가새가 좌굴하면 인장가새와 압축가새 사이에 수직불균형력이 발생하여 보와 기둥에 추가적인 하중이 가해지므로 이를 반영하여 보 및 기둥 부재를 탄성설계해야 한다. 지진하중 발생시에 모든 가새가 동시에 좌굴하지 않는다는 것은 잘 알려져 있지만, 특정층의 좌굴발생 유무를 정확히 예견하는 방법은 아직 존재하지 않는다. 따라서 현행 설계기준에서는 모든 층에서의 동시 좌굴을 가정하여 보수적으로 설계하거나 시스템초과강도계수로 증폭된 특별지진하중에 대해 기둥부재를 탄성설계하는 경험적이고 우회적인 방법을 제시하고 있다. 이를 개선하기 위한 첫 번째 단계는 우선 지진 내습시에 좌굴발생이 예견되는 층을 정확히 예측하는 것이다. 본 논문에서는 1차모드 푸쉬오버해석, 고차모드 푸쉬오버해석, 선형고유치해석에 의해 좌굴층을 예측한 후 이를 토대로 가새좌굴이 기둥에 가하는 축력을 산정하는 세 가지의 새로운 방법, 즉 FMPM(First Mode Pushover Method), MMPM (Multi-Mode Pushover Method), MSBM(Mode Shape Based Method)을 제안하였다. 이 세 가지 방안의 핵심은 좌굴 포텐셜이 높은 것으로 감지된 층의 수직불균형력은 선형합산하고 그렇지 않은 층의 수직불균형력은 SRSS(square root of sum of squares)법에 의해 조합하여 기둥에 가해지는 축력을 산정하는 것이다. 3층에서 15층에 이르는 5개의 골조모델에 대해 20개 지진가속도기록을 입력으로 한 방대한 비선형동적해석을 수행하여 제시한 방안의 타탕성을 검증하였다. 세 방법에 의한 기둥설계 결과는 모두 현행 설계기준의 방법보다 기둥의 물량을 대폭 줄이면서도 기둥부재가 탄성상태를 유지하여 역량설계법의 철학을 만족시켰다. 특히 MSBM은 간단한 선형 고유치해석결과만을 이용하지만 본 연구에서 가장 정확한 축력산정법인 MMPM과 큰 차이를 보이지 않을 정도로 정확하다. 실무 여건에서도 사용 가능한 방법으로 MSBM을 추천한다.

• Journal of Periodontal and Implant Science
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• 제25권1호
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• pp.133-145
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• 1995

The migration and proliferation of periodontal ligament cells are desired goal of periodontal regeneration therapy. PDGF and $TGF-{\beta}1$ are well known to regulate the cell activity of mesenchymal origin cell. The purpose of this study was to determine the effects of these growth factors on human gingival fibroblast and periodontal ligament cell actvity, and to identify the regulatory effect of $TGF-{\beta}1$ on the response to PDGF by MIT assay. Human gingival fibroblast and periodontal ligament cells were cultured from extracted teeth for non-periodontal reason. Cultured human gingival fibroblast and periodontal ligament cells in vitro were treated with polyperpetide growth factor PDGF and $TGF-{\beta}1$ in both a dose and time - dependent manner. Cell morphology were determined by inverted microscope and cell acitivity were determined by MIT assay. The result of this study demonstrated that PDGF and $TGF-{\beta}1$ were not changed the morphology of these cell compared with control group. PDGF or $TGF-{\beta}1$ increased cell activity of periodontal ligament cell in dose and time dependent manner but gingival fibroblast were decreased to the level of control group at third day. Additionally, incubation with $TGF-{\beta}1$ addition to PDGF resulted in a enhanced cell activity of PDGF. Therefore, cell acitivty of gingival fibroblast were not changed compared with control group. This stiudy demonstrates that PDGF and $TGF-{\beta}1$ are major mitogens for human periodontal ligament cell in vitro, and $TGF-{\beta}1$ is a regulator of cell activity to PDGF in human gingival fibroblast and periodontal ligament cell.

• 대한약리학회지
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• 제25권1호
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• pp.23-30
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• 1989

함께 투여되는 마약성진통제에 따라 효능제 또는 길항제(mixed agonist-antagonist)로 작용하는 buprenorphine을 사용하여 개구리 좌골신경에 존재하는 아편수용체의 성질을 검토하였다. 실험은 sucrose-gap apparatus를 사용하여 활동전압에 대한 영향을 관찰하였으며 약물의 상호작용을 검토하고자 meperidine 또는 naloxone을 사용하여 다음과 같은 결과를 얻었다. 1) Buprenorphine은 현저하게 개구리 좌골신경의 활동전압을 저하시켰다. 2) 두 개의 최고작용농도를 보였는데 저농도에서는 $10^{-8}\;M$, 또 고농도에서는 $10^{-4}\;M$에서 현저하게 작용을 나타내었다. 3) Buprenorphine은 meperidine의 활동전압 저하작용을 의의있게 억제하였다. 4) Naloxone에 의하여 Buprenorphine의 저하 작용이 억제되었다. 위의 실험결과로 Buprenorphine은 순수 마약성진통제의 작용을 억제하여 차단제로 작용하는 한편 또한 효능제로 활동전압을 의의있게 억제하였다. 이로써 개구리 좌골신경에 있는 아편수용체는 세포막에 또는 세포막 주위에서 $Na^+-Channel$이 활성화되는 데 중요한 역할을 하는 것으로 간주되며 이 수용체는 또한 Naloxone에 매우 민감한 반응을 나타낸다.

• 대한본초학회지
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• 제20권4호
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• pp.27-31
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• 2005

Objectives : The anticancer response of three different types of water extracts of Zingiberaceae Curcuma longa L. tested for sarcoma 180. Only few studies carried out to investigate the effects of other contents of Curcuma longa L. in anticancer activities, therefore, in this study we have investigated the effects of other component then curcumin in Curcuma longa L. for anticancer a activities. Methods : Three different types of water extracts of Curcuma longa L. were prepared as follows. The sarcoma cells (S180) were maintained in Dulbecco's modified Eagle's medium (DMEM) and were seeded on 24-well cell culture cluster flat bottom with lid tissue culture treated non-pyrogenic polystyrene. The growth of sarcoma 180 was monitored for 1, 2 and 5 days. The sarcoma cells were pictured using inverted microscope and cell density was counted using hemocytometry. Results : After 5 days in the culture medium the results showed high growth of sarcoma 180 for control condition and the surface of CCP plates were fully covered with the cells. In case of medium in which the 10% of filtered water extract of Curcuma longa L. was added a very limited growth of sarcoma 180 was observed. The results were showed only small difference in cell density for two different concentrations of unfiltered water extracts of Curcuma longa L. whereasin case of filtered water extracts the control of sarcoma growth shows better result. Conclusion : The filtered water extracts showed the best result relatively to the unfiltered water extracts for two different concentrations. This indicates that the water extracts of Curcuma longa L. can have anticancer activities possibly without curcumin.

• Journal of Korean Neurosurgical Society
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• 제57권1호
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• pp.1-5
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• 2015

Objective : Cerebral vessels, such as intracerebral perforating arterioles isolated from rat brain, have been widely used as an ex vivo model to study the cerebrovascular function associated with cerebrovascular disorders and the therapeutic effects of various pharmacological agents. These perforating arterioles, however, have demonstrated differences in the vascular architecture and reactivity compared with a larger leptomeningeal artery which has been commonly implicated in cerebrovascular disease. In this study, therefore, we developed the method for studying cerebrovascular function utilizing the olfactory artery isolated from the mouse brain. Methods : The olfactory artery (OA) was isolated from the C57/BL6 wild-type mouse brain. After removing connective tissues, one side of the isolated vessel segment (approximately $-500{\mu}m$ in length) was cannulated and the opposite end of the vessel was completely sealed while being viewed with an inverted microscope. After verifying the absence of pressure leakage, we examined the vascular reactivity to various vasoactive agents under the fixed intravascular pressure (60 mm Hg). Results : We found that the isolated mouse OAs were able to constrict in response to vasoconstrictors, including KCl, phenylephrine, endothelin-1, and prostaglandin $PGH_2$. Moreover, this isolated vessel demonstrated vasodilation in a dose-dependent manner when vasodilatory agents, acetylcholine and bradykinin, were applied. Conclusion : Our findings suggest that the isolated olfactory artery would provide as a useful ex vivo model to study the molecular and cellular mechanisms of vascular function underlying cerebrovascular disorders and the direct effects of such disease-modifying pathways on cerebrovascular function utilizing pharmacological agents and genetically modified mouse models.

• 한국광학회지
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• 제17권1호
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• pp.54-59
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• 2006

본 논문에서는 집적 광학 광도파로 소자 기술을 적용하여 생화학 물질의 성분을 정밀하게 측정 가능한 광소자로서 폴리머 광도파로와 브래그 격자를 이용하는 구조를 최초로 제안하였다. 유효굴절률법과 전송행렬법을 이용하여 최적의 감도를 가지는 브래그 격자 광도파로를 설계하였으며 코아와 하부 클래딩의 굴절률이 각각 1.540, 1.430인 폴리머를 이용하여 코아 두께가 $3{\mu}m$ 인 구조의 반전립 광도파로를 제작하였다. 코아 층까지 완성된 도파로 위에 레이저 빔 간섭계와 플라즈마 에칭을 이용하여 격자를 형성한 뒤 격자표면에 20 nm 두께의 Au층을 증착하고 칼릭사린(calixarene) 단분자층을 만들어 바이오센서를 제작하였다. 제작된 광센서를 이용하여 PBS(phosphate bufferedsaline) 용액에 함유된 $K^+$의 농도에 따라 브래그 반사픽이 단파장으로 이동하는 것을 관찰할 수 있었다.

• Restorative Dentistry and Endodontics
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• 제25권4호
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• pp.606-618
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• 2000

Dental pulp infection is most commonly caused by extensive dental caries, and some bacterial species invade root canals; bacterial components and products are thought to be associated with the pathogenesis of periapical periodontitis. A principle driving force behind pulpal disease response appears to lie in the host immune system's to bacteria and their products. We examined the production of interleukin $1{\beta}$ (IL-$1{\beta}$) and tumor necrosis factor ${\alpha}$(TNF-${\alpha}$) from human peripheral mononuclear cells, lymphocytes and monocytes stimulated by heat-killed Acitnobacillus actinomycetemcomitans (ATCC 29523), Porphyromonas gingivalis (ATCC 33277) and Prevotella intermedia (ATCC 25611), and also by their sonicated bacterial extracts (SBE), respectively. The effects of three strains of heat-killed bacteria and their SBEs on the morphology of cultured blood cell lines HL-60 (KCLB 10240) and J774A.1 (KCLB 40067) were observed under the inverted microscope. Ultrastructural changes of J774A.1 exposed to heat-killed P. intermedia and its SBE were investigated using transmission electron microscopy. Production of IL-$1{\beta}$ was reduced in human peripheral mononuclear cells after stimulation by sonic bacterial extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. Heat-killed and sonic extract of P. gingivalis inhibited the production of TNF-${\alpha}$ in peripheral mononuclear cells. Production of TNF-${\alpha}$ was inhibited in peripheral monocytes after stimulation by sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. HL-60 and J 774A.1 cells showed granular degeneration after treatment with heat-killed and sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia Chromatin margination and shrinkage were observed in 774A.1 treated with heat-killed P. intermedia. Cell wall structure and organelles were destroyed and vacuoles were formed in cytoplasm in J774A.1 treated with P. intermedia sonic extract. These results suggest that A actinomycetemcomitans, P gingivalis and P intermedia may have an important role in the formation and progression of pulpal diseases via both modulation of production of IL-$1{\beta}$ and TNF-${\alpha}$ from blood mononuclear cells and cytopathic effects.

• Wind and Structures
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• 제15권4호
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• pp.331-343
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• 2012

Thunderstorm downbursts are responsible for numerous structural failures around the world. The wind characteristics in thunderstorm downbursts containing vortex rings differ with those in 'traditional' boundary layer winds (BLW). This paper initially performs an unsteady-state simulation of the flow structure in a downburst (modelled as a impinging jet with its diameter being $D_{jet}$) using a computational fluid dynamics (CFD) method, and then analyses the pressure distribution on a solar updraft tower (SUT) in the downburst. The pressure field shows agreement with other previous studies. An additional pair of low-pressure region and high-pressure region is observed due to a second vortex ring, besides a foregoing pair caused by a primary vortex ring. The evolutions of pressure coefficients at five orientations of two representative heights of the SUT in the downburst with time are investigated. Results show that pressure distribution changes over a wide range when the vortices are close to the SUT. Furthermore, the fluctuations of external static pressure distribution for the SUT case 1 (i.e., radial distance from a location to jet center x=$D_{jet}$) with height are more intense due to the down striking of the vortex flow compared to those for the SUT case 2 (x=$2D_{jet}$). The static wind loads at heights z/H higher than 0.3 will be negligible when the vortex ring is far away from the SUT. The inverted wind load cases will occur when vortex is passing through the SUT except on the side faces. This can induce complex dynamic response of the SUT.

• 한국정보처리학회논문지
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• 제6권7호
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• pp.1832-1840
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• 1999

기존의 한국어 웹 정보 검색 시스템은 대부분이 불리언 검색 시스템이므로 사용자가 원하는 정보를 한 번의 질의에 의해 얻기가 매우 어렵다. 또한 생략이 빈번하고 링크가 많은 웹 문서의 특성상 기존의 역문헌 빈도에 의한 키워드 선정은 중의성의 문제를 가중시켜 부적절한 키워드가 추출된다. 따라서 원하는 정보를 얻을 때까지 사용자는 질의어의 수정을 반복한다. 본 논문에서는 이러한 문제를 해결하기 위해 연관 피드백(Relevace Feedback) 에이전트 시스템을 설계하고 구현하였다. 연관 피드백 에이전트 시스템은 사용자의 선호 키워드에 대한 적합 문서를 추출하여 선호 키워드를 선호 DB 테이블로 저장하였다가 사용자가 추후에 검색할 때 사용자 질의에 연관 키워드를 추가하여 검색한다. 이 결과로 사용자의 질의 수정의 횟수를 줄이고 검색 효율을 향상시킬 수 있었다.

• Earthquakes and Structures
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• 제17권2호
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• pp.175-189
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• 2019

The aim of the study is to show the results of complex shaking table experimental investigation focused on the response of two models of cylindrical steel tanks under mining tremors and moderate earthquakes, including the aspects of diagnosis of structural damage. Firstly, the impact and the sweep-sine tests have been carried out, so as to determine the dynamic properties of models filled with different levels of liquid. Then, the models have been subjected to seismic and paraseismic excitations. Finally, one fully filled structure has been tested after introducing two different types of damages, so as to verify the method of damage diagnosis. The results of the impact and the sweep-sine tests show that filling the models with liquid leads to substantial reduction in natural frequencies, due to gradually increasing overall mass. Moreover, the results of sweep-sine tests clearly indicate that the increase in the liquid level results in significant increase in the damping structural ratio, which is the effect of damping properties of liquid due to its sloshing. The results of seismic and paraseismic tests indicate that filling the tank with liquid leads initially to considerable reduction in values of acceleration (damping effect of liquid sloshing); however, beyond a certain level of water filling, this regularity is inverted and acceleration values increase (effect of increasing total mass of the structure). Moreover, comparison of the responses under mining tremors and moderate earthquakes indicate that the power amplification factor of the mining tremors may be larger than the seismic power amplification factor. Finally, the results of damage diagnosis of fully filled steel tank model indicate that the forms of the Fourier spectra, together with the frequency and power spectral density values, can be directly related to the specific type of structural damage. They show a decrease in the natural frequencies for the model with unscrewed support bolts (global type of damage), while cutting the welds (local type of damage) has resulted in significant increase in values of the power spectral density for higher vibration modes.