• International Journal of Industrial Entomology and Biomaterials
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• v.39 no.1
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• pp.14-21
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• 2019

Genome editing by CRISPR/Cas9, a third-generation gene scissor in molecular breeding at the genome level, is attracting much attention as one of the breeding techniques of the future. In this study, genetic and phenotypic analysis was used to examine the responsiveness of the Bakokjam variety of the silkworm Bombyx mori to molecular breeding using CRISPR/Cas9 in editing the white egg 2 (w-2) gene. The nucleotide sequence of the w-2 gene was analyzed and three different guide RNAs (gRNA) were prepared. The synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the Bm-N silkworm cell line. To edit the silkworm gene, W1N and W2P gRNA and Cas9 complexes were microinjected into silkworm embryos. Based on the results of microinjection, the hatching rate was 16-24% and the incidence of mutation was 33-37%. The gene mutation was verified in the heterozygous F1 generation, but no phenotypic change was observed. In F2 homozygotes generated by F1 self-crosses, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/Cas9 system is possible and will be a very effective way to shorten the time required than the traditional breeding process.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.155-160
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• 2007

In order to develop chili pepper bacterial spot resistant cultivars and near-isogenic lines (NILs) to prompt the molecular mapping of the resistance gene, we have run backcross breeding program since 1994. Two resistance genes against Xanthomonas axonopodis pv. vesicatoria Bs2 from Fla. XVR 3-25 and Bs3 from our breeding line 25-11-3-2, were introduced into a land race, Chilseongcho (abbreviated to Chilseong hereafter) with good fruit guality. We report here the testing of $BC_4F_3\;to\;BC_4F_5$. We found that $BC_4F_5$ lines of the crosses were homozygous with respect to the respective genes of introduction. The lines, in which Bs2 gene was introduced, were hypersensitively resistant to both race 1 and race 3 of X. axonopodis pv. vesicatoria, whereas, those in which Bs3 was introduced were resistant to race 1.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.2
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• pp.171-174
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• 1994

The milk production of Anglo-Nubian, Barbari and Barbari $\times$ Black Bengal goats were compared. The respective average lactation yield of those genotypes were $171.27\;{\pm}\;16.90$, $88.22\;{\pm}\;8.67$ and $52.31\;{\pm}\;13.51$ kg; mean annual milk yield $208.41\;{\pm}\;24.57$, $117.13\;{\pm}\;12.44$ and $84.19\;{\pm}\;23.45$ kg; per day milk yield $0.82\;{\pm}\;0.07$, $0.61\;{\pm}\;0.05$ and $0.43\;{\pm}\;0.06$ kg and lactation length $207.67\;{\pm}\;7.36$, $143.89\;{\pm}\;6.67$ and $116.75\;{\pm}\;13.86$ days. All traits recorded were influenced significantly (p<0.01) by the genetic group. Introduction of Barbari genes to Black Bengal goats had a positive effect in improving milk yield.

• Journal of Forest and Environmental Science
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• v.35 no.2
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• pp.115-120
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• 2019

There are many countries having a long history of exotic tree introduction from different countries and several countries including New Zealand, Brazil and Hungary have successfully introduced exotic trees. Korea's tree breeding program for introduction of exotic tree was originally started from 1924. Records reveal that hundreds of tree species were introduced from many countries and tested their germination and viability. About 415 introductions were eliminated since they had been introduced and tested. Subsequently, seven exotic trees that proved successful in trials and plantations have planted for afforestation. Simultaneously, several promising exotic trees are still under the test. In this paper, we will succinctly review and evaluate their comparative growth performance of the exotic trees in the plantation programs of the country to coping with climate change.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.41-62
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• 1996

Since 1966 tissue culture has been used as a tool for the production of disease indexed stocks from selected plants and their rapid (clonal) mass propagation through the procedure now referred to as micropropagation. The major advantages have been the rapid introduction of new plant cultivars, created within conventional and mutation breeding programmes, as healthy stock for beneficial distribution and the expansion of the world wide horticultural industry. (omitted)

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.23-23
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• 2023

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.63-67
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• 2013

A new silkworm variety "WhangBoJam" for spring rearing season is F1 hybrid between Jam317, a japanese strain bred from introduction breeding and Jam318, a chinese strain from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2012, the hatchability rate of WhangBoJam was recorded 95% similar to Kum HwangJam. The larval period was 8hours more long than KumHwangJam. The pupation percentage was recorded 6.6% higher than KumHwangJam. Single cocoon weight was recorded 2.29 g similar to KumHwangJam and cocoon yield(21.3 kg) was higher than KumHwangJam. The concentration of DNJ was measured to 0.22% in WhangBoJam, lower than DaePoongJam(0.26%). In the Paecilomyces tenuipes production ability test, the pupal weight of Whang BoJam was 1.33 g/individual, higher than KumHwangJam (1.19 g/individual).

• Journal of Sericultural and Entomological Science
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• v.48 no.2
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• pp.37-40
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• 2006

A new silkworm variety "Daepoongjam" for spring rearing season is $F_1$ hybrid between Jam151, a Japanese race bred from introduction breeding and Jam152, a Chinese race from introduction breeding. In the local adaptatability test performed at 8local areas in spring of 2005, the heavy cocoon yield of "Daepoongjam" was recorded 6% higher than "Kumokjam". The concentration of Deokynojirimycin (DNJ) was measured 4.22 mg in "Daepoongjam", similar to "Kumokjam" (4.26 mg). In the Peacilomyces tenuipes production ability test, the pupal weight of "Daepoongjam" was 1.61 g/individual 13% heavier than "Kumokjam" (1.41 g/individual)

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.1-4
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• 2012

A new silkworm variety "DaeBakJam" for spring rearing season is F1 hybrid between Jam155, a japanese race bred from introduction breeding and Jam156, a chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2010, the hatchability rate of DaeBakJam was recorded 96% similar to DaePoongJam. The larval period was 3 hour shorter than DaePoongJam. The pupation percentage was recorded 0.2% higher than DaePoongJam. Single cocoon weight (2.72 g) and Cocoon yield (25.4 kg) was higher than DaePoongJam. The concentration of DNJ was measured 3.28 mg in DaePoongJam, lower than DaePoongJam (3.48 mg). Test of the artificial diet of DaeBakJam showed bad adaptability of the artificial diet for larval period from 1st to 3rd, but KumOkJam showed excellent adaptability of the artificial diet. In the Paecilomyces tenuipes production ability test, the pupal weight of DaeBakJam was 1.69 g/individual, higher than DaePoongJam (1.59 g/individual).

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.5-9
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• 2012

A new silkworm variety SooOkJam for spring rearing season is F1 hybrid between Jam157, a japanese race bred from introduction breeding and Jam158, a chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2011, the hatchability rate of SooOkJam was recorded 2% higher than the authorized silkworm variety, DaePoongJam. The larval period was recorded 12 hour shorter than DaePoongJam. The pupation percentage was recorded 0.8% higher than DaePoongJam. Single cocoon weight (2.40 g), Cocoon yield (22.6 kg) and best cocoon rate (95.5%) was similar to DaePoongJam. The concentration of DNJ was measured 4.12 mg in SooOkJam, lower than DaePoongJam (4.45 mg). Test of the artificial diet of SooOkJam showed excellent adaptability of the artificial diet for larval period from 1st to 3rd, similar to KumOkJam. In the Paecilomyces tenuipes production ability test, the pupal weight of SooOkJam was 1.61 g/individual, similar to DaePoongJam (1.65 g/individual).