• Journal of Korea Multimedia Society
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• v.13 no.4
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• pp.575-581
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• 2010

With widespread use of the Internet and improvements in streaming media and compression technology, digital music, video, and image can be distributed instantaneously across the Internet to end-users. However, most conventional Digital Right Management are often not secure and not fast enough to process the vast amount of data generated by the multimedia applications to meet the real-time constraints. The SVC offers temporal, spatial, and SNR scalability to varying network bandwidth and different application needs. Meanwhile, for many multimedia services, security is an important component to restrict unauthorized content access and distribution. This suggests the need for new cryptography system implementations that can operate at SVC. In this paper, we propose a new scrambling encryption for reserving the characteristic of scalability in MPEG4-SVC. In the base layer, the proposed algorithm is applied and performed the selective scambling. And it encrypts various MVS and intra-mode scrambling in the enhancement layer. In the decryption, it decrypts each encrypted layers by using another encrypted keys. Throughout the experimental results, the proposed algorithms have low complexity in encryption and the robustness of communication errors.

• Bulletin of the Korean Chemical Society
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• v.32 no.4
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• pp.1158-1164
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• 2011

5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILICMS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column ($5{\mu}m$, $100{\times}2.1\;mm$). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 1-1000 ng/mL with linear correlation co-efficients ($r^2$) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.28 no.3
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• pp.32-43
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• 2014

This paper presents the intra capacitance modeling according to the winding method, section bobbin and coil structure for hybrid choke coil capable of the EMI attenuation of broad bands from lower frequency bands to higher frequency bands and high frequency type common-mode choke coil capable of the EMI attenuation of high frequency band used in the EMI Block of LED-TV SMPS. In case of high frequency type CM choke coil, it can be explained the parasitic capacitance of A type and section bobbin type winding methods among them is much smaller than the other. The first resonant frequency of the proposed CM choke coil tends to increase as the parasitic capacitance becomes small and its impedance characteristics also show improved performance as the first resonant frequency increases. In case of hybrid choke coil using rectangular copper wire, it has investigated its parasitic capacitance compared to CM choke coil of conventional toroidal type becomes small. Also it has confirmed through the experiment results that CE margin and RE margin in frequency bands 0.5MHz to 5MHz and 30MHz to 200MHz are respectively 10dB and 15dB greater than that of conventional type in case of one stage EMI filter structure adopting hybrid choke coil compared to two stage EMI Filter structure using two of each CM choke coil used in the lower and higher frequency bands or two of CM choke coil used in only the lower frequency bands. In the future, the hybrid choke coil and CM choke coil of high frequency type show it can be practically used in not only LED/LCD-TV SMPS but also several applications such as LED Lighting, Laptop Adapter, Server Power Supply and so on.

• Archives of Pharmacal Research
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• v.27 no.9
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• pp.901-905
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• 2004

A rapid, sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric(HILIC-MS/MS) method for the determination of tiapride in human plasma was developed. Tiapride and internal standard, metoclopramide were extracted from human plasma with dichloromethane at basic pH and analyzed on an Atlantis HILIC silica column with the mobile phase of acetonitrile-ammonium formate (190 mM, pH 3.0) (94：6, v/v). The ana-Iytes were detected using an electrospray ionization tandem mass spectrometry in the multi-ple-reaction-monitoring mode. The standard curve was linear (r＝0.999) over the concentration range of 1.00-200 ng/mL. The coefficient of variation and relative error for intra- and inter-assay at three QC levels were 6.4∼8.8％ and -2.0∼3.6％, respectively. The recoveries of tiapride ranged from 96.3 to 97.4％, with that of metoclopramide (internal standard) being 94.2％. The lower limit of quantification for tiapride was 1.00 ng/mL using 1 00 $\mu$L of plasma sample.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.651-654
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• 2005

Analysis of extra- and intra- cellular metabolites is very important to study cell metabolish. Intracellular amino acids in yeast are of great interest as precursors of desired products in biomass production of the feed industries and the production of glutamate. In this study, the fermentations of P. pastoris X-33 and KM71H were carried out in shake flasks. After centrifugation, the harvested cells were mechanically disrupted by using glass bead. The supernatants were used to analyze intracellular amino acid by HPLC system. For HPLC analysis, Resolve C18 column was used with Fluorescence detector. The OPA(o-phthalaldehyde) derivation reaction was employed for analysing amino acids at 30 $^{\circ}C$, 1 ml/min and gradient mode. The concentration of intracellular protein was measured by spectrophotometer.

• Archives of Plastic Surgery
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• v.33 no.4
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• pp.445-448
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• 2006

Purpose: The nose is the most prominent skeletal feature of the face and is thus prone to frequent injury. Closed reduction of nasal bone fractures can be performed under general or local anesthesia. However, the benefits and the drawbacks in either form of anesthesia chosen are seldom perceived by the surgeon. A retrospective study was performed to assess the differences in the outcome among the two groups subjected to surgery under different type of anesthesia and to introduce our method of local anesthesia and its adequacy. Methods: Two hundred and fifteen patients during a 2-year period were included in the study. 2% Lidocaine mixed with 1:100,000 epinephrine was injected on the anterior ethmoid nerve and the periosteum. Assessment factors included intra-operative adequacy of analgesia, post-operative analgesic requirement and functional and aesthetic outcome of surgery. Results: 19 patients were manipulated under general anesthesia and 196 patients were manipulated under local anesthesia on the anterior ethmoidal nerve and dorsal periosteum. No statistically signigicant variable in performance of surgery could be attributed to the mode of anesthesia employed(p > 0.05). Four patients experienced complications after reduction. One developed septal deviation and three nasal obstruction. But, no secondary operations were needed. Conclusion: Anterior ethmoidal nerve block and dorsal periosteal injection of 2% Xylocaine, combined with topical intranasal 4% lidocaine and epinephrine provided sufficient analgesia comparable to that of general anesthesia.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.15 no.3
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• pp.138-146
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• 2015

Purpose: If early diagnosis is not made, patients with metabolic disorders as homocystinemia rapidly progress to physical defect or mental retardation resulted in storage of the toxic material into the brain. Therefore, it is necessary to develop an analytical method for a rapid screening and/or correct confirmation diagnosis. Methods: The standard solution of sulfur amino acids spiked plasma was subjected to protein precipitation with methanol, and then consecutively derivatized with trimethylsilyl (TMS) and trifluoroacyl (TFA) and determined by GC-MS. The formation of TMS derivative of the hydroxyl and TFA derivative of amino functional group was performed by BSTFA and MBTFA, respectively. Selective ion monitoring (SIM) mode was used for quantification with selected specific ions. Results: A calibration curve on standard spiked pooled plasma showed a linear relationship with correlation coefficient of 0.9936-0.9992 for all compounds over the range of 0.1-300 ng. The precision and accuracy were within S.D. of 1-15% and RSD of 1-15% for intra-day assay at 2 ng/mL, 15 ng/mL and 30 ng/mL. LOD and LOQ was 0.4 ng/mL and 4 ng/mL respectively. Conclusion: A rapid analytical method was developed to quantify sulfur amino acids and methyl malonic acid, after two-step derivatization procedure with good sensitivity and specificity on human plasma. Advantages of a new method are simplicity and rapidity. The method could be useful for routine analysis, diagnosis of homocysteinemia.

• Natural Product Sciences
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• v.17 no.2
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• pp.147-159
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• 2011

A high-performance liquid chromatography-electrospray ionization-tandem mass spectrometric method was developed to determine simultaneously eight marker constituents of Forsythiae fructus, and subsequently applied it to classify its two botanical origins. The marker compounds of Forsythia suspensa were phillyrin, pinoresinol, phillygenin, lariciresinol and forsythiaside; those of F.viridissima were arctiin, arctigenin and matairesinol. Separation of the eight analytes was achieved on a phenyl-hexyl column (150${\times}$2.0 mm i.d., 3 ${\mu}M$) using gradient elution with the mobile phase: (A) 10% acetonitrile in 0.5% acetic acid, (B) 40% aqueous acetonitrile. A few fragment ions specific to the types of lignans, among the product ions generated by collisonally induced dissociation (CID) of molecular ion clusters, such as [M-H]$^-$ or [M+OAc]$^-$ were used not only for fingerprinting analysis but for the quantification of each epimer by using multiple-reaction monitoring mode. It was shown good linearity ($r^2{\geq}$ 0.9998) over the wide range of all analytes; intra- and inter-day precisions (RSD, %) were within 9.14% and the accuracy ranged from 84.3 to 115.1%. The analytical results of 40 drug samples, combined with multivariate statistical analyses - principal component analysis (PCA) and hierarchical cluster analysis (HCA) - clearly demonstrated the classification of the test samples according to their botanical origins. This method would provide a practical strategy for assessing the authenticity or quality of the herbal drug.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.11a
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• pp.172-175
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• 2011

높은 효율을 가진 비디오 압축 코덱인 H.264/AVC는 예전보다 압축 성능을 향상 시키기 위한 방법 중 하나로 율-왜곡 최적화 기법이라는 것을 사용한다. 이 기법은 압축된 결과를 가지고 손실과 압축률 두 가지를 모두 고려하여 어떤 경우가 더 최적의 압축인가 하는 것을 판별하는데, 여기서 모든 경우에 대해 압축을 수행해야 함으로 이전 보다 몇 배나 높은 복잡도를 가질 수 밖에 없다. 이러한 문제를 해결하고자 하는 노력으로 본 논문에서는 SAHTD(Sum of Absolute Hadamard Transform Difference)라는 기준을 사용하여 이를 이용해 율-왜곡 최적화 기법을 사용하지 않거나, 사용을 최소화 하면서 압축 효율을 유지하는 방법을 제시하였다. 본 논문에서는 휘도 신호 $8{\times}$8과 $4{\times}4$블록을 위한 방법을 제시하고 있다. 이 두 가지 크기의 블록에 대해서 SAHTD값을 구해 SAHTD가 낮은 순으로 모드들을 정렬하고, 이것이 가장 낮은 3개의 모드를 선택해 이것 중에 MPM이 포함되지 않았을 경우에 대해서는 MPM을 포함해 4개의 모드를 선택해 압축을 수행하도록 한다. 여기서 얻은 모드들 중에 SAHTD값이 일정 값 이상 더 낮은 모드가 존재할 경우, 그 모드들에 대해서만 율-왜곡 최적화 기법을 수행한다. 이를 통해 최적의 모드일 가능성이 낮은 모드들에 대한 부가적인 연산 수행을 방지하게 된다. 휘도신호 16x16이나 색차 신호 압축의 경우에는 SAHTD를 사용하여 가장 낮은 SAHTD값을 갖는 모드를 최적의 모드로 결정한다. JM 참조 소프트웨어를 통한 실험결과 제안된 기법은 기존 JM의 방식에 비해 화면 내 프레임의 부호화 시간의 82.2% 감소와 0.042dB의 PSNR 감소율, 0.527%의 비트 증가율을 보여주었다.

• Bulletin of the Korean Chemical Society
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• v.33 no.1
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• pp.177-182
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• 2012

A new composition of standardized Scutellariae Radix extract (HPO12) was developed for treatment of Alzheimer's disease. For the preclinical pharmacokinetic study of HPO12, a rapid, sensitive, and selective LCMS/MS method was developed and validated for the simultaneous determination of 4 bioactive compounds, baicalein, baicalin, wogonin, and wogonoside. After extraction with ethylacetate, chromatographic analysis was performed on a Thermo $C_{18}$ column ($150mm{\times}2.1mm$, $3{\mu}m$) with a mobile phase consisting of 0.1% formic acid (A) and 0.1% formic acid in 95% acetonitrile (B) by using gradient elution at a flow rate of $250{\mu}L/min$. Analytes introduced to a mass spectrometer were monitored by multiple reaction monitoring (MRM) in positive ion mode. Using $25{\mu}L$ of plasma sample, the method was validated over the following concentration ranges: 25-5000 ng/mL for baicalein, 20-40000 ng/mL for baicalin, 1-1000 ng/mL for wogonin, and 5-10000 ng/mL for wogonoside. The intra- and inter-day precision and accuracy of the quality control samples at the 4 concentrations showed $\leq$ 13.7% relative standard deviation (RSD) and 86.6-105.5% accuracy. The method was successfully applied to determine the concentrations of baicalein, baicalin, wogonin, and wogonoside in rat plasma after intraperitoneal and oral administrations of HPO12.