• Journal of Medicine and Life Science
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• 제20권3호
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• pp.135-138
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• 2023

• 한국환경성돌연변이발암원학회지
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• 제24권1호
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• pp.19-24
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• 2004

In order to understand the mechanism of the regulation of drug metabolizing enzyme gene expression, we have studied the induction of CYP1A1 and $GST\alpha,$ $\mu,$ $\pi$ enzymes in Japanese monkey and rhesus monkey after the treatment with 3-methylcholanthrene (3MC) and di-n-butyl phthalate (DBP) and bisphenol A (BPA). The levels of mRNA were measured by RT-PCR in brain, intestine and liver. In the case of adult monkey, treatment with 3MC induced CYP1A1 mRNA in intestine by 11-fold. The treatment with DBP induced CYP1A1 mRNA. Effects of 3MC and DBP on GST mRNA expression was not clear. But $GST\mu$ was slightly inhibited by the treatment with 3MC and DBP. $GST\alpha$ was induced in intestine by 1.5-fold. $GST\pi$ was slightly induced by the treatment with 3MC and DBP in intestine. In the case of fetus monkey, the basal levels of fetus CYP1A1 mRNA and GSTs mRNA were relatively low compared to adult monkey. As the age of monkey increased, the basal levels of CYP1A1 mRNA were also increased. 3MC induced the expression of CYP1A1 mRNA didn't significantly induce CYP1A1 mRNA in intestine. The levels of $GST\mu$ and $GST\pi$ were not changed by the treatment with 3MC and DBP. $GST\pi$ was slightly induced by the treatment with 3MC and DBP.

• Journal of Pharmaceutical Investigation
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• 제37권2호
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• pp.73-78
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• 2007

The aim of this study was to investigate the absorption properties of ketoprofen. The in-situ perfusion model has advantages over in vitro models as it provides intact lymphatic and blood flow circulation. The absorption properties of six different concentrations of ketoprofen have been studied in single pass in-situ rat intestine model. $^{14}C-PEG$ 4000 was used as a permeability marker and the possibility of an energy dependent contribution to ketoprofen absorption was also Investigated using the metabolic inhibitor sodium azide. Three different concentrations of sodium azide were studied to examine its effect on absorption of ketoprofen from the rat intestine. The findings of this study suggest that mono-carboxylic type drugs like ketoprofen cause permeability changes in the intestine. This is shown by the increase in absorption of $^{14}C-PEG$ 4000 as the concentration of ketoprofen is increased. However, the trend for ketoprofen permeability is to decrease over the concentration ranges. It was observed that the Papp values for ketoprofen with sodium azide shows a trend towards reduction in the amount of ketoprofen absorbed from the rat intestine which was significantly different (p<0.05) from that of ketoprofen with sodium azide 3.0mM. This indicates that sodium azide has an affect on the absorption of ketoprofen. The pH of all the perfusion solutions was altered to ${\sim}pH\;6.7$ by the buffering capacity of the small intestine secretions. The results suggest that mechanisms other than passive diffusion may be involved in ketoprofen absorption. This would be consistent with the involvement of active transport or saturatable processes in the absorption of drugs containing monocarboxylic acid group, as has been previously suggested from in vitro data.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.841-847
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• 2005

The objective of this experiment was to investigate the development of gastrointestinal tract and activities of pancreatic enzymes in White Roman geese. Thirty developing embryos at the 22th, 24th and 26th day of incubation and at hatching, and sixteen or eight goslings, half males and half females, at the 1, 3, 7 or 11, 14, 21 and 28 days of age were sampled, respectively. The weights of the yolk, gastrointestinal tract and intestinal length, and the activities of pancreatic enzymes were measured. Residual yolk weight decreased rapidly during late incubation and was nearly depleted at 3 days of age. The protein and energy contents in the residual yolk of goslings at 3 days of age were significantly (p<0.05) less than those at the late incubation. From 6 days before hatching to 28 days of age, the absolute weights of gizzard, proventriculus, liver, pancreas, small intestine and large intestine in goslings increased by 48, 457, 94, 2334, 89 and 76 times, respectively. The relative weights of proventriculus, gizzard, liver, pancreas, small intestine and large intestine reached peaks at 3, 3, 14, 14, 11 and 11 days of age, respectively, and then decreased gradually. However, the relative lengths of small intestine and large intestine reached peaks at 3 days of age and at hatching, respectively. The activities of pancreatic trypsin and chymotrypsin increased sharply from hatching to 14 day of age, and then decreased gradually until 21 days of age. The activity and specific activity of pancreatic amylase were increased following by age and peaked at 7 to 11 and 21 days of age, respectively. The activity and specific activity of pancreatic lipase reached a plateau from 11 to 28 days of age. These results indicate that the gastrointestinal tract and activities of pancreatic enzymes developed more rapidly than body weight through the early growing period of goslings.

• 한국어류학회지
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• 제7권2호
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• pp.140-149
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• 1995

망상어, Ditrema temmincki 소화관의 형태 조직화학적인 특징은 다음과 같이 요약할 수 있다. 체장에 대한 식도에서 항문까지의 소화관 길이의 비 (RLG)는 0.89정도이며, 소화관에는 식도와 담관 입구사이의 팽창부인 위(胃)가 없다. 소화관은 형태 조직화학적인 특징에 의하여 식도, 식도 - 장이행부, 전장, 중장, 후장, 장 - 직장 이행부, 직장, 직장 - 항문 이행부, 항문으로 나눌 수 있다. 점막주름은 직장에서 가장 복잡한 형태를 보이며, 근육층은 식도와 항문에서 발달된 상태를 나타낸다. 점막주름의 상피층은 식도 전방부에서는 입방세포로 구성되며, 나머지 부위 에서는 원주상피로 구성된다. 소화관에는 PAS 반응에 양성을 나타내는 배상세포와 다당류 흡수세포 (Polysaccharid Absorptive Cell)가 관찰되는데, 후자는 다당류 계통의 영양물질의 흡수기능을 가진다. 망상어 소화관에서 다당류 계통의 영양물질은 주로 후장부에서 흡수된다.

• 센서학회지
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• 제17권1호
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• pp.1-8
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• 2008

Recently, capsule endoscope was developed to observe small intestine in human body. However, the capsule does not have any locomotive ability, which reduces the benefit of the capsule endoscope. Many researches have done to give locomotion to the capsule, still it consumes too much power to generate the motion by small battery. One of the moving method is electrical stimulus that consumes less power than many methods. The electrical stimulus method causes contraction in the small intestine, and it moves the capsule. Some of papers showed it is possible to guide the capsule by electrical stimulus, however the relationship between electrical stimulus at the mucous and contraction force in the small intestine is not reported, yet. In this paper, the mucous in the small intestine was stimulated, and measured the contraction force in the small intestine is shown. The result shows, the relationship between electrical stimulus parameters (voltage, duration) and contraction force. Also, equation between electrical stimulus parameters and contraction force is roughly induced.

• 동의생리병리학회지
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• 제26권6호
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• pp.947-952
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• 2012

This Study was conducted to investigate Muscle Test of Point Selection through CRA(Contact Reflex Analysis) Muscel Diagnosis. So this study used to compare and analyze the effects of Muscel(Deltoid Muscel of Posterior) RMS(Root Mean Squared) and MEF(Median Edge Frequency) Among Groups that do not respond to questionnaire, Tonguibogam Naegyeongpyeon Small Intestine Group, Small Intestine MeridianPathway Primary Symptom and Secondary Symptom Group and Kwanwon(CV4) meridian Principal action Group. The questionnaire is composed of 23 items. The questionnaire was intended to elicit information on assorting into 4 groups. After Survey, Subject had to Muscle test subjects. Muscle experimental methods are as follows: Holding the shoulder without contacting Kwanwon. Holding the shoulder contacting Kwanwon. The first iteration. Group 1,2,3 were increased sEMG RMS compared with First experiment and Second experiment. Group 4(Including Uterus and Intestinal Flora Problem) were decreased sEMG RMS compared with First experiment and Second experiment. This test means that it is similar to diagnosis CRA and Small intestine channel of hand taiyang muscle, not Small Intestine MeridianPathway. It is suggested that subjects with a Small Intestine problem(Uterus and Intestinal Flora Problem) shows the results of decreasing posterior Deltoid Muscular strength. It means that CRA muscle diagnosis is associated with Alarm points diagnosis. But it doesn't consider influence of fat on the surface EMG.

• 대한한의학방제학회지
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• 제27권4호
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• pp.237-244
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• 2019

Objectives : The purpose of this study was to investigate the effects of Carthami flos on pacemaker potentials of small intestinal and colonic Interstitial Cells of Cajal (ICC). Methods : To dissociate the ICC, we used enzymatic digestions from the small intestine and colon in mice. In the ICC, the electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICC. Results : 1. The ICC generated pacemaker potentials in the murine small intestine and colon. 2. Pretreatment with a Ca²⁺ free solution and thapsigargin, a Ca²⁺-ATPase inhibitor in the endoplasmic reticulum, stopped the pacemaker potentials. In the case of Ca²⁺-free solutions, Carthami flos did not induce membrane depolarizations in the murine small intestine and colon. However, when thapsigargin in a bath solution was applied, Carthami flos induced membrane depolarizations only in the murine colon. 3. Pretreatment with 2-APB (transient receptor potential melastatin (TRPM) channel inhibitor) abolished the pacemaker potentials and suppressed Carthami flos-induced effects in the murine small intestine and colon. 4. However, pretreatment with T16Ainh-AO1 (Ca²⁺ activated Cl- channel; anoctamin 1 (ANO1) inhibitor) did not affect the pacemaker potentials and induced Carthami flos-induced effects only in the murine small intestine. Conclusions : These results suggest that Carthami flos can modulate the pacemaker activity of ICC and the mechanisms underlying pacemaking in ICC might be different in the small intestine and the colon.

• 혜화의학회지
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• 제14권1호
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• pp.67-81
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• 2005

We have conclusions after the study of muscular system about small intestine channel of hand taiyang muscle. Judging from many studies of interrelation between Meridian muscle and muscle, it is considered that Meridian muscle theory has some similarities with modern anatomical muscular system. It is considered that Small intestine channel of hand taiyang muscle contains Flexor digitorum profundus muscle, Extensor digiti minimi muscle, Abductor digiti minimi muscle, Extensor carpi ulnaris muscle, Flexor carpi ulnaris muscle, Triceps brachii muscle, Infraspinatus muscle, Levator scapulae muscle, Sternocleidomastoid muscle, Masseter muscle, Temporalis muscle. The symptoms of small intestine channel of hand taiyang muscle is similar to referred pain of modern Myofascial Pain Syndrome, and the medical treatment of "I Tong Wi Su(以痛爲輸)" is also similar to that of Myofascial Pain Syndrome. Small intestine channel of hand taiyang muscle is one of the three yang channels of hand muscle, and it has unity in extension of upper limb and trunk in the movement. And it is thought that weakness of small intestine channel of hand taiyang muscle is related with muscular system causing Round Shoulder and Head Forward Position.

• Fisheries and Aquatic Sciences
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• 제27권2호
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• pp.122-135
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• 2024

Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.