• The Korean Journal of Mycology
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• v.41 no.4
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• pp.261-267
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• 2013

In order to select antagonists for biological control of downy mildew of cucumber, 126 bacteria were isolated from cucumber plants collected from several locations in Korea. Among them, Five isolates were selected as potential biocontrol agents of cucumber downy mildew using a leaf disc bioassay method. In preventive and curative effect tests, the isolate CC110 was found to be effective to control downy mildew on cucumber showing diseased area by 0% whereas that of control was 15.0~18.0%. A bacterium isolate CC110 was identified as Bacillus amyloliquefaciens subsp. plantarum based on phylogenetic analysis using gyrB gene sequence. The culture liquid of isolate CC110 in TSB media were more effective for the control of the disease than those cultured in LB, NB, and KB media in leaf disk bioassay. when undiluted liquid, two-fold, five-fold diluted culture broth, and undiluted liquid, two-fold, five-fold diluted filtrate of isolate CC110 in TSB media were treated, diseased area of cucumber powdery mildew were 0%, 3.0%, 8.0%, 0%, 4.0% and 7.0%, respectively, whereas diseased area in the control was 21.0%. In the cucumber seedling tests, when the culture broth of isolate CC110 in TSB media was treated, diseased area were 35.0%, whereas that of control was 82.0%. When B. amyloliquefaciens CC110 was treated four times at five-day interval in the vinylhouse test, the control effect of cucumber downy mildew was higher than that treated three at seven-day interval.

• Korean Journal Plant Pathology
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• v.13 no.3
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• pp.167-171
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• 1997

To find optimum application methods of Epicoccosorus nematosporus for control of water chestnut, five different concentration of conidial suspensions ($10^[3}$ conidia/ml to $10^[7}$ conidia/ml) fo the fungus were applied 1 to 4 times on 10~40 days old seedlings of water chestnut in greenhouse. Inoculum levels equal to or greater than $10^[5}$ conidia/ml killed significantly more shoots (82.6%~92.1%) and suppressed significantly underground tuber formation compared to inoculum concentration less than $10^[4}$ conidia/ml. When the conidial suspension of ($6.3 {\times} 10^{5}$conidia/ml of E. nematosporus was sprayed 2 times in 7 days interval, percentage of the killed plants was up to 98.7%. Numbers of reshoots and tubers were alsosuppressed significantly compared to one time application. The percents of killed shoots were similar between 10-day-old and 20-day-old seedlings, and significantly higher than those treated on 30- or 40-day-old seedlings. The fungus treated on 20-day-old seedlings was the most effective because there was high number of reshoots from 10-day-old seedlings. Therefore, optimal application conditions for E. nematosporus is 2~3 times of application in 7 days interval with $10^[5}$ conidia/ml on 20-day-old seedling age.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.327-334
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• 2006

In tomato perlite bag culture, the available water content was analyzed for several sorts of perlite so that the irrigation safety was investigated and thus the irrigation strategy controlled by time clock was established. The reduction trends of water content in perlite bags were monitored for tomatoes, of which fruits were harvested until the fifth cluster. The amount of daily reduction of water in the bag was on the decrease as the total water in the bag was decreased. In terms of time interval from when the water content based on weight was reduced more than 50g to when it was dropped again, the longest time interval in a day was retarded gradually. It means plant activity was recovered later than the previous day. The available water content in perlite bag of 40 liters was about 30% which was 12 kg in weight, which satisfied daily water demand of 6 tomato plants. The appropriate time irrigated by time clock was recommended for the case that it was irrigated 5 or 10 times a day with the daily integrated solar radiation of 601 or $1,519W/m^2$.

• Journal of Korea Water Resources Association
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• v.48 no.9
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• pp.711-717
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• 2015

This paper attempts to measure the economic benefits of advanced water treatment in five cities (Goyang, Paju, Gumi, Gimcheon, Chilgok), which are supplied water from Goyang and Gumi filtration plant. We used the dichotomous choice contingent valuation method to estimate WTP. Parametric interval-data model are used to obtaining the mean WTP estimates. The results show that the mean of additional WTP for advanced water treatment services were estimated to be KRW 231.3 and KRW 231.2 per $m^3$ using model with covariates and without covariates, respectively. Given the water supplies of Goyang and Gumi filtration plants ($59.675m^3/y$ and $93.734m^3/y$), the economic benefits of those advanced water treatments can be expected to be KRW 13.8 billion and KRE 21.68 billion. And the calculated B/C ratios are 3.7 and 2.1 when a lifespan of facility is 10 years. Advanced water treatment should be introduced in terms of the economic benefits and costs. Thus, this results can be useful in water policy decision-making.

• Journal of Wetlands Research
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• v.23 no.4
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• pp.296-306
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• 2021

Elasticity is a statistical technique that interprets the changing pattern of another variable according to a change in one variable as a quantitative numerical value and provides more information than correlation analysis and is widely used in climate change research. In this study the elasticity was calculated and sensitivity analysis was performed using air temperature and water quality data of the major tributaries of the Nakdong River. In addition the confidence interval for the elasticity was calculated using the T-Test and the validity of the elasticity was examined. The strength of elasticity shows high strength in the order of summer>fall>spring>winter and the direction shows regional characteristics with both negative and positive elasticity. After performing hierarchical cluster analysis on monthly observation data they were classified into 5 clusters and the characteristics of each cluster were visually analyzed using a parallel coordinate graph. The direction and intensity of the air temperature elasticity show regional characteristics due to the relatively high population density and complex influencing factors such as sewage treatment plants, small-scale livestock houses and agricultural activities. In the case of TP it shows great regional variability according to the circulation of nutrients in the ecosystem caused by algae growth and death according to temperature changes. Since the air temperature elasticity of the major tributaries of the Nakdong River is over weak and is valid at the significance level of 5%, it was analyzed that there is a change in water quality according to the air temperature change.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.187-192
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• 1996

This study was investigated to know about the stages of flower bud development and the effects of natural and artificial cold treatment on flowering of herbaceous peony. Developing buds of Paeonja lactㅑflora Pall. var. Taebaek were observed since Jun. 17 and peony plants were forced since Nov. 27 in the green house with two weeks interval, and other plants were forced after cold treatment in $5^{\circ}C$ for 1, 2, 4, 6weeks. Differentiation of vegetable part in peony buds was started in early June, and floral part was differentiated in September and their differentiation was continued to shooting in early spring. Buds of peony were sprouted and flowered when it was forced on Dec. 4. Days to shooting were decreased with delay of forcing time from early to late of December, significantly. Two weeks for cold treatment were enough to break dormancy of peony and days to shooting of the cold treated were significantly shorter than the untreated in the same forcing times

• Korean Journal of Environmental Agriculture
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• v.18 no.1
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• pp.41-47
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• 1999

To investigate the combined effect of gamma ray irradiation and NaCl treatment on Tradescantia somatic cell pink mutations, potted plants of Tradescantia 4430 were evenly sprayed with NaCl solution(170mM) 24 hours before irradiation(NaCl+${\gamma}$) and after irradiation(${\gamma}$+NaCl). Irradiation doses were 0.3, 0.5, 1.0 and 2.0 Gy of gamma-ray. The plants irradiated only with gamma radiation were used as control group(CT). Frequency of pink mutation increased linearly with irradiation close and the peak interval of elevated mutation frequencies appeared during 6∼12 days aver irradiation in all the experimental groups. The slope of dose-response curve in CT was 5.99($r^2$=0.99), while it were 4.55($r^2$=0.98) in NaCl+${\gamma}$ and 4.33($r^2$=0.99) in ${\gamma}$+NaCl. It seemed that pre- and post-treatment of NaCl had a protective effect it against radiation-induced cell damages since it decreased the slope value by more than 24%. It is suggested that protective effect on DNA damages can be invoked in irradiated stamen hair cells by NaCl treatment.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.98-98
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• 2017

Leaf senescence is the process of aging in plants. Chlorophyll degradation during leaf senescence has the important role translocating nutrients from leaves to storage organs. The functional stay-green with slow leaf yellowing and photosynthesis activity maintenance has been considered one of strategy for increasing crop productivity. Here, we have identified two QTLs on chromosome 9 and 10 for leaf senescence with chlorophyll content of RIL population derived from a cross between Hanareum 2, early leaf senescence Indica-type variety, and Unkwang, delayed leaf senescence Japonica variety. Among these QTLs, we chose qPLS1 QTL on chromosome 9 for further study. qPLS1 was found to explain 14.4% of the total phenotypic variation with 11.2 of LOD score. Through fine-mapping approach, qPLS1 QTL locus was narrowed down to about 25kb in the marker interval between In/del-4-7-9 and In/del-5-9-4. There are 3 genes existed within 25kb of qPLS1 locus: LOC_Os09g36200, LOC_Os09g36210, and LOC_Os09g36220. Among these genes, transcript level of LOC_Os09g36200 was increased during the leaf senescence stage and the expression level of LOC_Os09g36200 in Indica was higher than in Japonica. Finally, we chose LOC_Os09g36200 as candidate gene and renamed it as OsPLS1-In and OsPLS1-Jp from Indica- and Japonica-type rice, respectively. OsPLS1-In and OsPLS1-Jp overexpressing transgenic plants showed both early leaf senescence phenotype. These results indicate that OsPLS1 functions in chlorophyll degradation and the difference of expression level of OsPLS1 cause the difference of leaf senescence between Indica and Japonica in rice.

• Korean Journal of Environmental Biology
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• v.17 no.1
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• pp.21-26
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• 1999

To investigate the combined effect of radiation and pesticide on Tradescantia somatic cell mutations, potted plants of Tradescantia 4430 on which parathion had been sprayed evenly 24 hours before irradiation. Radiation doses were 0.3, 0.5, 1.0 and 2.0 Gy of gamma-ray. The plants irradiated only with the gamma-ray radiation were used as control groups (CT). Pink mutation frequency increased linearly proportional to the radiation dose and the peak interval of elevated mutation frequencies appeared during 7 ~ 11 days after irradiation in both CT and Pa +${\gamma}$ groups. The slope of dose -response curve in CT was 5.99 ($r^2$= 0.988), while it was 3.43 (r$x^-2$=0.981) in Pa+${\gamma}$. It seemed that parathion pretreatment had a protective effect against radiation-induced cell damages since it decreased the slope value by 43%. It is suggested that an adaptive response or radiomodification could be induced in irradiated stamen hair cells by parathion pretreatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.471-475
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• 1987

Some physiological analysis and the trend of a change in mineral nutrient contents in 2 years old ginseng plants were investigated for 10 times at 15 to 25 days interval from April 25th to October 10. The growth of leaf in size was completed by the beginning of June and that of stem in height by the late of June. The trend of a seasonal change in root weight showed a form of V letter, that is, the weight was de-creased until late of May and increased continuously thereafter up to September. However, the duration of root growth might be dependent on the defoliation. The growth of root in length begins from the beginning of June and stops in the beginning of August. Relative growth rate, net assimilation rate and crop growth rate were all negative values in just after shoot-emergence. The values showed a peak in May and decreased gradually thereafter. The leaf area ratio showed a peak of 76$\textrm{cm}^2$/g in May, and down to 30$\textrm{cm}^2$ /g in the beginning of October. The contents of nitrogen and potassium in aerial part of ginseng plants decreased at the late growing season, but increased on lime. No seasonal changes on phosphate and magnesium were observed. The contents of nitrogen, phosphate and potassium in root decreased rapidly at the maximum growing season for the aerial part and increased gradually thereafter. Whereas no changes on lime and magesium were observed during the growing season.