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Change in the Seed Characteristics and Germination Properties of Ulmus davidiana var. Japonica According to Seed Collection Time (채취 시기에 따른 느릅나무의 종자 형질 및 발아 특성 변화)

  • Tak, Woo Sik;Choi, Chung Ho;Kim, Tae Su
    • Journal of Korean Society of Forest Science
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    • v.95 no.3
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    • pp.316-322
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    • 2006
  • This study was conducted to examine the changes of seed characteristics and germination properties and to determine the most suitable collection time in Ulmus davidiana var. japonica. Seeds were collected six times with the interval of seven days after April 21, 2005 at Jeong-seon. There were differences in seed characteristics and germination properties among seed collection times. The sizes of seed and fruit increased with the collection time, but seed moisture contents decreased with the collection time after increasing until May 5. The 1,000 seeds weight decreased with the collection time after increasing until May 12. Collection time showed high positive correlations (r=0.790, p<0.01) with seed size and negative correlations (r=-0.919, p<0.01) with moisture contents. Percent germination (PG) presented the highest value in seeds collected at May 5, and decreased with the collection time. Mean germination time(MGT), germination speed(GS), and germination performance index(GPI) also showed the highest values in seeds collected at May 5. PG, MGT, and GS had correlations with other seed characteristics except the 1,000 seed weight. The relative growth rate of height and root collar diameter of seedlings presented the highest values in seedling germinated from seed collected at May 5, and seedlings germinated from seeds collected at May 5 and 12 showed the highest values in chlorophyll contents. Consequently it will be possible to product a number of seedlings when the seeds of U. davidiana var. japonica are collected just before drying morphologically.

Passive Immunity by Splenocyte Transfer against Amebic Meningoeneephalitis in Mice (세포에 의한 아메바성 수막뇌염에 대한 피동면역의 전달)

  • 임경일;유재숙
    • Parasites, Hosts and Diseases
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    • v.26 no.3
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    • pp.169-174
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    • 1988
  • The role of passive cell-mediated transfer of immunity against primary amoebic meningoen- cephalitis(PAME) in mice was studied. Waegleria fowleri, ITMAP 359, were cultured in CGVS medium. The ICR mice used were six week-old males of average weight of 15 g. Immunization was done by three intraperitoneal injections of $1{\times}10^6$ N. fowleri trophozoites at the interval of one week. Splenocytes were obtained from normal and immune mice spleens, and Ix107 cells were administered intraperitoneally into mice 3 days before challenge infection. Mice were infected intranasally with $7{\times}10^4$ N. fowleri trophozoites in a $3{\;}{\mu}l$ suspension under secobarbiturate anesthesia. Transplants of normal or immune splenocytes seem to alter the pattern of the PAME level- opment. The splenocytcs transferred from immune mice reduced the mortality rate in the JV. fowleri infected mice, as compared with the mice transferred with the same number of normal splenocytes or without splenocyte, The blastogenic response of the splenocytes to both lipopoly- saccharide and concanavalin A was elevated on duty 7 after infection the mice transinoculated with immune splenocytes. The serum antibody titers in the mice transferred with immune spleno- cytes were increased gradually from day 7 up to day 20 after infections by mean of ELISA. It is suggested that the transfer of splenocytes from immuniged mice conferred immunity against N. fowleri infection.

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A Clinical Study on the Effects of Myocardial Protection of St. Thomas Hospital Cardioplegic Solution During Open Heart Surgery (개심술에서 St. Thomas Hospital 심정지액의 심근보호효과에 관한 임상적 연구)

  • Kim, Yeong-Hak;Kim, Geun-Ho
    • Journal of Chest Surgery
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    • v.22 no.2
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    • pp.225-233
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    • 1989
  • Cardioplegia and myocardial protection were performed under cardiopulmonary bypass during open heart surgery with the use of St. Thomas Hospital cardioplegic solution [4 [C] for the coronary artery perfusion and normal saline solution [4[ c] for the topical cardiac cooling. To maintain the state of myocardial protection, coronary artery reperfusion was carried out using St. Thomas Hospital cardioplegic solution at the interval of 30 minutes. A total number of patients studied were 57 cases, including 37 cases of correction for congenital anomalies and 20 cases for acquired heart diseases. Cardiopulmonary bypass time during the surgery was observed to be average of 87.89*47.55 hours, aortic cross-clamping time [ACCT] to be average of 76.68*44.27 hours raging from 30 to 191 minutes. In order to evaluate the effects of myocardial protection in the surgery, serum enzyme levels were determined. To observe the relationship between ACCT and myocardial protection effects, patients studied were divided into the following 3 groups. I group: ACCT 60 minutes, II group: ACCT 90 minutes, III group: ACCT over 91 minutes [1] SGOT; The positive value [increased over 200 units] for ischemic myocardial injury during operation was observed in 11 cases [19.3% of the total] of the total patients studied, of which 4 cases [13.3%] in I group, 1 case [10.0%] in II group, and 6 cases [35.3%] in III group. [2] LDH; The positive value [increased over 900 units] for ischemic myocardial injury during operation was observed in 9 cases [15.7% of the total] of the total patients studied, of which 2 cases [6.6%] in I group, 1 case [10.0%] in II group and 6 cases [35.3%] in III group. [3] CPK; The positive value [increased over 800 units] for ischemic myocardial injury during operation was observed in 10 cases [17.5% of the total] of the total patients studied, including 4 cases [13.3%] in I group, 1 case [10.0%] in II group, and 5 cases [29.4%] in III group [4] The myocardial protection method used in the present study was demonstrated to be effective for the myocardial protection in the surgery with ACCT of up to 90 minutes. A few ischemic myocardial injury were observed in the surgery with ACCT over 91 minutes, but no significant cardiac dysfunction was noted. The surgery with ACCT of up to 191 minutes did not appear to give rise any significant interference with postoperative recovery.

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Immune Reaction of the Vaccinated Hamsters with Combined Hantaan-Puumala Vaccine (신증후출혈열의 혼합백신을 접종한 햄스터에서의 면역성 조사)

  • Lee, Ho-Wang;Chu, Yong-Kyu;Cui, Long-Zhu;Woo, Young-Dae;Ahn, Chang-Nam;Kim, Hoon;Jang, Yang-Seok
    • The Journal of Korean Society of Virology
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    • v.27 no.1
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    • pp.39-47
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    • 1997
  • A large number of viruses belonging to Genus Hantavirus in Family Bunyaviridae are etiologic agents for hemorrhagic fever with renal syndrome (HFRS), or hantavirus pulmonary syndrome (HPS). Hantaan (HTN), Seoul (SED), Belgrade (BEL), Puumala (PUU) serotype viruses are well known causative agents for HFRS in Eurasian continent. Among those viruses Hantaan and Seoul serotypes are well known to cause HFRS in Korea, but there are some sporadic incidence by other than Hantaan or Seoul viruses. Recently we have developed the combined Hantaan-Puumala virus vaccine to prevent world-wide occuring HFRS. This combined vaccine is formalin inactivated, suckling mouse and suckling hamster brain extracts for Hantaan and Puumala viruses, respectively. Protein contents of this purified candidate vaccine is $27\;{\mu}g/ml$, which contains 1,024 ELISA antigen units to each virus, but content of myelin basic protein which is causing experimental allergic encephalomyelitis is less than 0.1 ng/ml. Thirty hamsters were given twice at one month interval intra-muscularly and bled on 30 days after each vaccination from retro-orbital sinus vein. Antibody titers were tested against 5 major serotype viruses, Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses by IFA and PRNT. The mean IF antibody titers on 30 days after primary shot were 78.4, 68.8, 68.8, 37.9, and 15.6; mean neutralizing antibody titers were 65.4, 12, 6.1, 65.6 and 0.5 against Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses, respectively. The mean IF antibody titers on 30 days after booster shot were 686.9, 567.5, 550.4, 516.3, and 430.9; and neutralizing antibody titers were 710.8, 41.9, 24.3, 409.9, and 1.6 against Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses, respectively.

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Multiple Period Forecasting of Motorway Traffic Volumes by Using Big Historical Data (대용량 이력자료를 활용한 다중시간대 고속도로 교통량 예측)

  • Chang, Hyun-ho;Yoon, Byoung-jo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.38 no.1
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    • pp.73-80
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    • 2018
  • In motorway traffic flow control, the conventional way based on real-time response has been changed into advanced way based on proactive response. Future traffic conditions over multiple time intervals are crucial input data for advanced motorway traffic flow control. It is necessary to overcome the uncertainty of the future state in order for forecasting multiple-period traffic volumes, as the number of uncertainty concurrently increase when the forecasting horizon expands. In this vein, multi-interval forecasting of traffic volumes requires a viable approach to conquer future uncertainties successfully. In this paper, a forecasting model is proposed which effectively addresses the uncertainties of future state based on the behaviors of temporal evolution of traffic volume states that intrinsically exits in the big past data. The model selects the past states from the big past data based on the state evolution of current traffic volumes, and then the selected past states are employed for estimating future states. The model was also designed to be suitable for data management systems in practice. Test results demonstrated that the model can effectively overcome the uncertainties over multiple time periods and can generate very reliable predictions in term of prediction accuracy. Hence, it is indicated that the model can be mounted and utilized on advanced data management systems.

Effects of Castration and Administration of Testosterone Propionate on the Thyroid Gland, Adrenal Gland and Testis in Immature Male Rats (미성숙 수흰쥐에 있어서 생식선척출 및 propionate testosterone 투여가 갑상선, 부신 및 정소조직에 미치는 영향)

  • 김종호;정영채;김창근
    • Korean Journal of Animal Reproduction
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    • v.4 no.1
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    • pp.35-45
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    • 1980
  • The purpose of this experiment was to investigate the effects of castration and administration of testosterone propionate(TP) on the development of the thyroid, adrenal glands and the testis in immature male rats, 25 days immatured, weighing 64.1${\pm}$2g, were divided into two groups of control and castrated, each sub-divided into 30 rats again, treated and untreated with TP respectivity. Each rat was given 20$m\ell$ of TP subcutaneously at two weeks interval. Six rats among each group were randomly sacrificed at 7, 21, 35, 49, and 63 days after treatment, of which their thyroid, adrenal glands and testis were collected for cytometric observation. The results obtained were as follows. 1. The size of the follicle of thyroid glands had a tendency to increase proportionally to the treatment period in every group. However, in castration group, the follicular size of the untreated with TP were significantly increased from 49 days (p<0.05) after treatment than that of the treated with TP, while in control group, the treated with TP were not increased during the treatment period. Regarding the height of the follicular epithelial cell in thyroid gland, the treated with TP had a tendency to increase than the untreated with TP in both castration and control group. 2. Regarding the size of the follicle of thyroid gland in relation with the increment period, the untreated with TP in control group were slightly increased from 49 days after treatment, but the treated with TP were not changed significantly. Castration group had a tendency to increase significantly than the control group, especially the untreated with TP in castration group were significantly increased. 3. As for the change of the relative height of thyroidal follicular epithelial cell in relation with the increment of treatment period, the untreated, A and C group, in both castration and control group were increased at 35 days 63 days after treatment while the treated, B and D group had tendency to increase from 21 days after treatment. 4. Regarding the thickness fo adrenal cortex, the castration group had a tendency to increase than the control group until 21 days after treatment. But, at 35 days, the change of the thickness was reversed; Mean while at 49 days and 63 days, especially C group in castration were significantly increased than any other groups although there were no significant differences among the every group during the whole treatment period. Regarding the thickness of adrenal cortex in relation with the increment of treatment period, A, B and C group had a tendency to increase until 21 days after treatment. After that period, there was no significant increment in all groups. Especially, in D group, there were no significant changes from 7 days to 63 days after treatment. 5. As for the tickness of adrenal medulla, there were no significant changes in every group of castration and TP treatment, except that the castration group had a tendency to increase continually than the control throughout the whole treament period. 6. In terms of the number, diameter and thickness of seminiferous tubule in testis of control group, the treated group with TP were distinctly reduced than those of the untreated group from 49 days after treatment respectively.

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Establishment of Perfect-Drainage Period for Reduction of Salt Injury and Improvement of Grain Filling Ratio in the Newly Reclaimed Land (신간척지에서 염해경감 및 등숙률 향상을 위한 완전낙수시기 구명)

  • Choi, Weon-Young;Yang, Chang-Hyu;Lee, Jang-Hee;Kim, Taek-Kyum;Jeong, Jae-Hyeok;Cho, Min-Kyu;Kim, Si-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.177-181
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    • 2011
  • This research was carried out to establish perfect-drainage time in order to stabilize rice yield and improve rice quality. Treatments of perfect-drainage were conducted 5 days interval during 25 days to 50 days after heading date in the field of Saemangeum Gyehwa, newly reclaimed land. Accumulated temperature after heading date in 2010 increased about $100^{\circ}C$ and precipitation amount decreased a little compared to normal year harvesting time. Average panicle number was 16.5 and spikelet per panicle was 88. Perfect drainage time treatment after 40~50 days was 3% higher in percent ripened grain and 0.6 g heavier in 1,000 grain weight than treatment after 25~35 days. There was no difference of rice yield between perfect drainage time treatment after 25 days and 30~35 days, but rice yield was 7~8% higher in treatment after 40~50 days than 25 days. Head rice ratio in treatment after 35 days was the highest and the sooner perfect drainage time, the lower protein content. Soil moisture negatively correlated with soil hardness and EC in this result. With this results, we proposed that the time of perfect drainage in newly reclaimed land to stable rice production is 40~50 days after heading date.

Impact of Changes in Medical Aid Status on Health Care Utilization

  • Kim, Woorim;Nam, Chung Mo;Lee, Sang Gyu;Park, Sohee;Kim, Tae Hyun;Park, Eun-Cheol
    • Health Policy and Management
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    • v.29 no.4
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    • pp.513-522
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    • 2019
  • Background: South Korea operates a Medical Aid (MA) program targeting selected low-income individuals to ensure medical service delivery to the disadvantaged while enhancing self-sufficiency of work-capable beneficiaries. However, as reasons behind welfare exits are diverse and do not always infer poverty relief or the provision of appropriate levels of health care services, this study aimed to investigate the association between changes in MA status and health care utilization. Methods: This study used the 2006 to 2015 National Health Insurance claims data. The impact of changes in annual MA status on health care utilization (yearly number of outpatient visits, inpatient visits, length of stay, and emergency department [ED] visits) was investigated using the generalized estimating equation model. Results: In 117,943 adult subjects aged 20 to 64, compared to the 'MA to MA' group, the 'MA to MA exit' group showed general decreases in utilization (outpatient visits: β=-3.93, p<0.0001; hospital admissions: relative risk [RR], 0.87; 95% confidence interval [CI], 0.83-0.91; length of stay: β=-3.64, p<0.0001; ED visits: RR, 0.83; 95% CI, 0.77-0.90). Similar patterns were found in the 'MA exit to MA exit' group (outpatient visits: β=-5.72, p<0.0001; admissions: RR, 0.91; 95% CI, 0.87-0.94; length of stay: β=-5.87; p<0.0001; ED visits: RR, 0.81; 95% CI, 0.75-0.88). Likewise, in 74,747 older adult subjects aged 65 or above, the 'MA to MA exit' group showed reduced levels of utilization (outpatient visits: β=-1.51; p=0.0020), as well as the 'MA exit to MA exit' group (admissions: RR, 0.92; 95% CI, 0.89-0.95; length of stay: β, -5.45; p<0.0001; ED visits: RR, 0.90; 95% CI, 0.83-0.97). Conclusion: MA exit was associated with general decreases in health care utilization. Utilization patterns of individuals with experiences of receiving MA benefits should be monitored to promote the ideal use of health care services while preventing potential financial barriers present in accessing medical care.

Relationship between Physical Function Factors and Discrimination Experiences of the Elderly in Korea: The 2014 and 2017 National Survey on Living Conditions and Welfare Needs of Korean Older Persons (우리나라 노인의 신체기능 요인과 차별경험의 관련성 연구: 노인실태조사(2014, 2017) 자료 분석)

  • Lee, Kyuhee;Yong, Wangshik;You, Changhoon;Lee, Yongjae;Chung, Woojin
    • Health Policy and Management
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    • v.29 no.4
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    • pp.454-468
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    • 2019
  • Background: Supporting the elderly population is presented as a social issue, and it affects age discrimination, which forms a negative perception and avoids the elderly. Since age discrimination lowers the quality of life of the elderly and hinders social unity, it may be important to research related factors. This study examined the physical function as a factor that influences discrimination experiences and aimed to identify the relationship between physical function factors and discrimination experiences. Methods: In this study, we analyzed 20,225 elderly from the 2014 and 2017 survey of living conditions and welfare needs of Korean older persons, conducted nationwide among older than 65 years. Physical function factors are activities of daily living, instrumental activities of daily living, sight discomfort, hearing discomfort, chewing discomfort, and leg strength as factors of interest. We performed multivariable logistic regression that reflected survey characteristics, adjusting for socio-demographic factors, health related factors, and social-support factors. Results: The number of elderly who faced discrimination experiences was 1,175 (5.8%). The results showed that the odds ratio of facing a discrimination experience in the dependent group was significantly higher compared to the independent group when being transferring out of the room (4.05; 95% confidence interval [CI], 1.50-10.88) and difficulty in hearing (1.25; 95% CI, 1.05-1.49). Even with respect to chewing ability, which was significant in models 1 and 2, they face more discrimination experiences (1.30; 95% CI, 1.11-1.53). Conclusion: These research results indicate that an important strategy for preventing age discrimination is to understand the physical function conditions of the elderly and promote the physical functions of the elderly related to transferring, hearing, and chewing; it is necessary to develop a practical intervention plan that considers these aspects.

Fishing Mechanism of Pots and their Modification 3. Behavior of Conger Eel, Astroconger myriaster, to Net Pots (통발어구의 어획기구 및 개량에 관한 연구 3. 그물통발유에 대한 붕장어의 행동)

  • KIM Dae-An;KO Kwan-Soh
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.3
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    • pp.238-244
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    • 1990
  • The behavior of conger eel, Astroconger myriaste(Brevoort), to the net pots with baits was investigated alternately in two experimental water tanks. One of the pots being dropped on the tank bottom, the eels increased rapidly in number of touching the pot to show a maximum whithin 30 minutes and then decreased gradually. The enterings of eels into the pots were also made mainly within 30 minutes and easier in pots with lower entrances. But the complete enterings were decided by the passage of eel tails through the pot entrances and so the eels retreated frequently after attemping to enter the pots, especially in pots small in the interval between the entrances and the baits or in pots with flappers. However, most of enterings encouraged the touches and earlier enterings ascribed earlier production of maximum touch. The eels, if entered the pots without flappers, got out of the pots relatively easily and the getting-out was easier in pots with lower entrances. On the other hand, the eels entered the pot with flappers could not get out of the pots. A comparison between the circular and square pots gave that the guidance of eels to the entrances was easier in the former and the passing of eels through the entrances in the latter, not only at the inside of pots but also at the outside. The general behavior of eels in touching or entering the net pots in the above results was almost equal to that on the bamboo and plastic pots in the previous paper.

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