• Title/Summary/Keyword: internode

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Shoot regeneration from internode sections of Ardisia pusilla DC.

  • Lee, Su-Young;Kim, Young-Soon;Han, Bong-Hee
    • Journal of Plant Biotechnology
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    • v.35 no.3
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    • pp.209-213
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    • 2008
  • This study was conducted to regenerate shoots from internode sections(about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body(PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 $mg{\cdot}L^{-1}$ thidiazuron(TDZ) and 0.5 $mg{\cdot}L^{-1}$ indole-3-butyric acid(IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.

Dynamic Mechanical Properties of Bamboos in Korea (한국산(韓國産) 죽재(竹材)의 동력학적(動力學的) 성질(性質))

  • Hong, Byung-Wha
    • Journal of the Korean Wood Science and Technology
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    • v.16 no.1
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    • pp.45-54
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    • 1988
  • This study was carried out to investigate the fiber length, fiber width and fiber thickness on the longitudinal direction by the parts of culm and also specific gravity in air dry, dyna.mic mechanical properties and internal frictions by the internode and the node in Phyllostachys bambusoides Phyllostachys nigra var. henonis and Phllostachys edulis which were grown in Korea. The results obtained were as follows; 1. The variations of fiber length and fiber width on the longitudinal direction had a tendency to increase slightly from the butt and then to decrease toward the top. but there was not a definite variation for fiber thickness according to the parts of culm. 2. The specific gravity in air dry of internode was increased from the butt toward the top, but the specific gravity in air dry of node was not correlations with the parts of culm. 3. Dynamic Young's modulus of internode on the longitudinal direction was increased according to the heights of culms within each species, and there were in order of P. edulis P. nigra var. henonis and P. bambusoides. 4. Correation coefficients between the specific gravity and the dynamic Young's modulus were 0.837 in P. bambusoides 0.871 in P. nigra var. henonis and 0.935 in P. edulis and there was also highly significant for dynamic Young's modulus between the specific gravities in air dry. 5. There were not correlations between the internal frictions and the parts of internode.

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Differentiation of Reaction Tissues in the First Internode of Acer saccharinum L. Seedling Positioned Horizontally (수평으로 위치한 은단풍(Acer saccharinum L.) 유식물의 제1절간에 있어서 반응조직의 분화)

  • 강경덕
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.211-217
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    • 1992
  • In order to elucidate the formation of reaction tissues during the transition from primary to secondary growth, the developmental anatomy was conducted in the first internode of Acer sacchan'num seedling in horizontal position. During the transition from primary to secondary growth, tension wood(gelatinous fiber) was gradually developed on the upper side only, And the tension wood formation in the upper side of the horizontal first internode proceeds acropetally from base to apical portion. Some of the anatomical features of tension wood start to be in the primary vascular tissue and a typical tension wood show during the secondary growth, Therefore, the procambium seems to respond to the gravity as well as vascular cambium. For this reason, both procambium and vascular cambium has to regard as the same meristem, On the other hand, the upper side vessels were longer than those of the lower side in the horizontal first internode. The lateral-wall pitting of vessel elements, however, showed no differences between upper and lower sides which have alternate type. The width and height of rayon the upper side of horizontal first internode was larger as compared with the lower side.r side.

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Callus Induction and Plant Regeneration from Stolon in Zoysiagrass (한국잔디류에서 포복경 배양을 통한 캘러스 유기와 재분화에 관한 연구)

  • 김종보;박순정;김두환
    • Asian Journal of Turfgrass Science
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    • v.11 no.4
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    • pp.311-320
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    • 1997
  • This study was carried out to induce and maintain callus from 59 zoysiagrass lines, to know the effective disinfestation method for zoysiagrass stolon as explant and the difference in the response of callus induction among 59 lines, and to investigate the effect of medium, growth regulators, light, temperature, stolon part and internode position on callus induction and emhryogenic callus(E.C.) formation. The treatment of 0.lmg/L $HgCl_2$for 15 min resulted in no contamination and the highest callus induction(46.6%). Callus was induced from the 59 zoysiagrass lines. The callus growth of Z. japonica and Z. sinica was generally better than Z. matrella Ten cell lines whose callus and stolon grow fast in culture and in field, respectively were selected to he used for breeding. Callus induction was the most effective at 2.0mg /L of both 2, 4-D and picloram in MS medium. MS medium was the best for callus induction and growth while LS medium was the best for embryogenic callus and shoot formation. Callus induction and growth was better at 28, 31$^{\circ}C$. than 25$^{\circ}C$. and dark condition was better than light condition in MS me-dium containing 2mg/L 2,4-D. While callus induction was better with node part as explant than with internode part, callus growth and embryogenic callus formation was better with internode part. In 'Japonica 1', the first internode was the most effective in callus induction, but third internode was the best in '$M_2$ X $S_2$'.

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STUDIES ON THE GENETIC ANALYSIS AMONG BURLEY, FLUE-CURED AND SUN-CURED TYPE TOBACCO. III. GENETIC COMPONENTS IN $F_1$ GENERATION. (버어리종, 황색종, 양건종, 담배의 유전분석에 관한 연구 III. $F_1$의 유전분석)

  • 한철수;김용암;정기택;이종두;권구홍
    • Journal of the Korean Society of Tobacco Science
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    • v.9 no.1
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    • pp.45-55
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    • 1987
  • For some basic information in tobacco breeding, the modes of inheritance and heritabilities for the twelve agronomic and chemical characters were estimated in the study of eight varieties partial diallel set. Additive gene actions were significant for all characters except total nitrogen content and dominance gene effects were also significant for all characters. Yield, number of leaves per plant, leaf length and leaf shape index were inherited as partial dominance, and overdominance was detected for plant height, stem diameter, internode length, total alkaloid, total nitrogen and total sugar content. Dominant gene showed increasing effects in yield, plant height, stem diameter, internode length, leaf width and total sugar content, but showed decreasing effects in the other characters. The broad heritability was very high for all characters. and the narrow heritability was high in days to flower and yield, but low in internode length, total alkaloid, total nitrogen and total sugar content.

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Effects of Gibberellin and Phytochrome B on Internode Elongation in Sorghum (수수의 절간 신장에 미치는 지베렐린과 파이토크롬 B의 영향)

  • 이인중;김길웅;모간페이지
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.5
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    • pp.548-555
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    • 1997
  • Sorghum seedlings lacking one of the phytochromes, phyB, have elongated internode, suggesting that they may have an alteration in gibberellin physiology. To test the possibility that phyB mutations affect seedling gibberellin perception and metabolism, the responsiveness of wild-type and phyB-1 seedlings to exogenous $GA_3$ was investigated. The phyB-1 showed higher internode elongation rate than the wild type in response to lower concentrations of exogenous $GA_3$ application, showing that the mutation causes an increase in responsiveness to GA. However, at the higher concentrations of $GA_3$ application, phyB-l and wild-type showed similar elongation rate, impling that responsiveness to higher concentrations of GA is not controlled by phytochrome. These results suggest that, although GAs are required for internode elongation, phyB does not act primarily by changing absolute levels of GAs but rather by decreasing seedling responsiveness to GAs at lower concentrations.

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Ultrastructure and Histochemisty of Deteminate Vascular Cambium in Rhizome of Botrycnium tematum (고사리삼 지하경에서 유한 유관속 형성층의 미세구조 및 조직화학)

  • 소웅영
    • Journal of Plant Biology
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    • v.38 no.1
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    • pp.115-119
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    • 1995
  • In order to clarify the relationship between the activity and age of vacular cambium in the rhizome of Botrychium ternatum, the cambia of the 6th and 10th internodes from its shoot apex were compared ultrastructurally and histochemically. The cambium in the 6th internode had two to four radially arranged cells in each row, and the cells are radially flattened and had thiner tangential walls than radial. The cambial cells were ultrastructurally characterlized by the presence of a large vacuole, few storage materials such as starch grains and lipid droplets, and various cell organelles such as mitochondria, endoplasmic reticulums and dictyosomes in cytoplasm. On the other hand, the cells of cambium in the 10th internode showed irregular arangement and somewhat enlargement in radial plane. The cells were filled with numerous storage materials and small vacuoles and had few cell organelles except for mitochondria. In addition, the activity of acid phosphatase appeared in the cambium of the 6th internode but of the 10th. From the above results, it is clear that the vascular cambium of the 6th internode was active and that of the 10th was dormant. Thus we reached a conclusion that the rhizome of '||'&'||'trychium ternatum has determinate cambium.

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Relationship among Elongation Periods of Young Spikes, Internodes and Leaf-sheaths in Wheat (Triticum aestivum L.) (소맥의 유수, 절간 및 엽초의 신장시기와의 관계)

  • 김석동;하용웅
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.3
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    • pp.238-242
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    • 1982
  • The growth of spike, internode and leaf sheath in winter wheat(Tritican vulgale Vill.) was investigated by measuring them 10 times from April 15 to May 30, with 5 days intervals, in 1980. The variety used was Glumil(Suweon #215) which was newly developed at Wheat and Barley Research Institute in Suweon, Korea. During the periods investigated, the spike elongated from 0.6cm to 8.3cm. The spike began to elongate rapidly from 20 days before heading (April 25) and finished nearly off the elongation at 5 days before heading (May 10). The length of the spike showed highly significant correlation with that of 3rd internode ($r=0.974^{***}$), and. with that of first leaf sheath($r=0.954^{***}$) from the top of wheat plant. Correlation between the length of the 3rd internode and that of the first leaf sheath was also very high($r=0.995^{***}$). A major elongation of the spike occurred concurrently with the elongation of the third internode and with that of the first leaf sheath.

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Plant Regeneration from Leaf and Internode Segment Cultures of Boxthorn (Lycium chinense Mill.) (구기자나무의 잎과 마디절편체 배양에 의한 식물체 재생)

  • 김동찬;정해준;민병훈;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.329-333
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    • 2001
  • Callus and shoot formation from medicinal crop, Lycium chinense Mill. cv. 'Cheongyang', as influenced by various media, explant sources and plant growth regulators were investigated. The rate of shoots formation, number of shoots, and fresh weight of shoots were the best on MS medium followed by B$_{5}$, WPH, and SH. Callus induction was more effective in leaf than internode segments, and was the best on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA. Effects of plant growth regulators in shoot formation were more effective in BA than TDA combined with NAA. Shoot formation from callus induced in leaf and internode segments was the best on MS medium containing 0.01 mg/L NAA with 0.2 mg/L BA.

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Effect of Daylength on the Panicle Exsertion of Panicle Enclosing 'Gamadi' Rice Cultivar (일장조건이 수도 GAMADI 품종의 이삭 추출에 미치는 영향)

  • G. L, Shrestha;M. H, Heu;S. Z, Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.2
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    • pp.121-124
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    • 1981
  • The panicle enclosing Gamadi rice cultivar from western Tarai of Nepal was treated with different daylengths; 8 hours, natural daylength i.e. 14 hours 30 minutes to 12 hours 30 minutes, and 24 hours throughout its life cycle. The extremely long daylength of 24 hours induced the panicle exsertion, and elongated the first internode from the top, but did not affect the second internode significantly, suggesting that the panicle exsertion was strongly correlated with the first internode elongation in the Gamadi rice cultivar.

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