• Journal of Chest Surgery
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• 제6권1호
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• pp.89-94
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• 1973

Esophagel atresia and tracheoesophageal fistula may occur as separate entities but usually occur in combination. First described by Durston in 1670, esophageal atresia was not successfully treated until 1939 when Ladd in Boston and Leven in St. Paul obtained the first survivors utilizing the methods of gastrostomy, esophagostomy and extrapleural ligation of the tracheoesophageal fistula as multiple operations which required months of hospitalization. Two years later Cameron Haight performed the first successful primary repair and afterward about 2000 cases of esophageal atresia with distal tracheoesophageal fistula reported in the world. In Korea, there appeared about 27 cases in the literature and 8 successful repaired cases noted in these year. Anther report two cases of esophageal atresia, of which one case was successfully treated with Haight`s method. Case 1.: Normal full term delivered boy with chief complaints of respiratory difficulty and persistent drooling with chocking, 3. lkg, was admitted with emergency 5 hours after delivery. Physical findings revealed no specific abnormal signs except distended abdomen and grunting respiration. Esophagograrn and bronchogram revealed proximal esophageal atresia and distal tracheoesophageal fistula proximal to the carina. Parent refused operative therapy and patient died 24 hours after discharge. Case 2. :3. lkg. normal full term delivered girl was admitted 4 days after delivery with chief complaints of regurgitation after feeding, chocking, cyanotic spell and fever since the day after delivery. Physical examination revealed persistent drooling, grunting respiration, and fever with moderate dehydration. Tracheoesophageal suction and fluid therapy with antibiotics improved her condition and subsided ]pneumonic condition. Esophagogram revealed markedly dilated proximal esophagus as blind loop and stomach distended with gas, and repairing operation as Haight`s method was performed on the 7th day after delivery. Patient tolerated all the operative procedure well and recovered uneventfully. Esophagogram on the 7th postoperative day showed passage of the lipiodol through the anastomotic side with moderate stricture,and feeding permitted. Patient tolerated all the feeding amount well and discharged on the 11th postoperative day. Followup revealed intermittent regurgitation after feeding and corrected with bougination.

• 한국환경생태학회지
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• 제28권5호
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• pp.516-522
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• 2014

본 연구에서는 두루미류의 서식밀도에 영향을 주는 요인 중 인위적 요인에 의한 영향을 평가하고자 하였다. 대상지역은 강원도 철원군의 민간인통제지역이며 2009년부터 2014년까지 매년 1월의 두루미와 재두루미의 분포를 대상으로 하였다. 인위적 요인인 도로, 거주지 및 군사시설로 부터의 거리에 따른 두루미류의 밀도를 파악하였으며, 비닐하우스의 분포밀도 차이에 따른 두루미류의 서식밀도를 평가하였다. 두루미와 재두루미는 동일지점에서 서식밀도 상관관계가 있었기 때문에 인위적 요인에 대해 유사한 민감성을 보이는 것으로 나타났다. 거주지와 가까운 곳은 두루미와 재두루미 모두 낮은 서식밀도를 보였으며, 2.5km 구간까지 거리가 멀어질수록 밀도가 증가하는 양상을 보였다. 이는 군사시설 및 통행량이 높은 도로와 유사한 양상이었으며, 군사시설로 부터는 약 0.8km까지, 통행량이 높은 도로는 2km 구간까지 요인으로부터 멀어질수록 서식밀도가 증가하였다. 이러한 경향은 인가지역과 군사시설 및 통행량이 높은 도로가 두루미류의 서식밀도에 일정범위까지 악영향을 주는 것을 의미하였다. 통행량이 적은 도로와 인접한 곳은 간헐적 간섭으로 먹이자원이 보전되어 있어 밀도가 높은 것으로 판단되었다. 비닐하우스의 밀도가 낮은 곳에서는 밀도가 증가할수록 두루미류의 서식밀도가 감소하는 경향을 보였는데, 비닐하우스의 밀도가 40개/$km^2$ 미만인 경우에서 나타났다. 하지만 비닐하우스의 밀도가 40개/$km^2$ 이상인 지역에서는 뚜렸한 밀도의 감소가 나타나지 않았다.

• Journal of Ecology and Environment
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• 제32권3호
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• pp.197-206
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• 2009

Benthic macroinvertebrate communities were collected from six different sites in the Dobong Stream in Seoul, Korea to investigate spatial and temporal changes in benthic macroinvertebrate communities in response to natural disturbances such as floods and droughts. We collected samples monthly or semimonthly with a Surber net ($30cm{\times}30cm$), and measured environmental factors, including stream temperature, discharge, width, conductivity, dissolved oxygen and pH at each sampling site. Benthic macro invertebrates were strongly affected by floods as well as droughts. In addition, benthic macroinvertebrate communities displayed different responses to the onset of the rainy season in summer 2006 and 2007, apparently due to differences in the intensity and amount of precipitation. Chironomids were particularly sensitive to heavy rain. Floods and droughts also affected the proportions of functional feeding groups during the survey period: the proportion of scrapers was high right after heavy rains, while the proportion of predators tended to increase in intermittent-type streams as the riffle zone decreased. Finally, although species richness and abundance were strongly influenced by heavy rain, they recovered to background levels for within one month, and varied consistently among stream types, indicating habitat stability.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.81-84
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• 2001

A real time optimization algorithm for fed-batch cultures of recombinant yeast to determine the optimal substrate feed rate profile has been developed. Its development involved four key steps: (1) development of reliable adaptive model. (2) development of optimization algorithm. (3) design of on-line model update algorithm to be incorporated into the optimization algorithm and (4) experimental validation. A recombinant Saccharomyces cerevisiae producing human parathyroid hormone (hPTH) was chosen as the model strain. It was found to be very successful in maintaining cell growth and galactose consumption at leigh levels, thus resulting in significant improvements in the productivity (up to 2.1 times) and intact hPTH concentration (up to 1.5 times) compared with the case of an intermittent glucose and galactose, or galactose feeding.

• Journal of Chest Surgery
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• 제16권1호
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• pp.127-130
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• 1983

Esophageal atresia and Tracheoesophageal fistula may occur as separate entities but usually occur in combination. First described by Durston in 1970, esophageal atresia was not successfully treated until 1939 when the first two survivors of staged correction were described by Ladd and Leven. In 1941, Haight and Towsley performed the first successful primary repair. Authors report four cases of esophageal atresia of which two cases were treated surgically in success with Haight`s method. The type of four cases were all the same as upper blind pouch and lower tracheoesphageal fistula. Two of them were associated with verterbral defect, imperforate anus and/or rib fusion. Two cases died within seven days due to parent`s refusal for operative therapy, others were treated surgically with Haight`s method. Operative patients tolerated all the operative procedure and recovered uneventfully, permitted feeding on 7th postoperative day. On follow up study, one patient revealed intermittent regurgitation and corrected with bougienation another with good health without complication.

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.220-228
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• 1995

For polyhydroxyalkanoic acid (PHA) production, several microorganisms were isolated from sewage sludge. One of them, GB-77 strain, was chosen from its PHB/HV copolymer production on only fructose without cosubstrate. The isolated strain GB-77 was identified as the genus Alcaligenes. Optimal temperature and pH for cell growth were 36C and 6.8. Optimal medium composition was 10 g/l of fructose and 5 g/l of polypeptone, 1 $\times$ 10$^{-2}$M Na$^{2}$HP0$^{4}$, 1.3 $\times$ 10$^{-2}$M KH$^{2}$PO$^{4}$. To investigate the optimal condition for polyhydroxyalkanoic acid production two-stage culture technique was used; first stage for cell growth and second stage for PHA production on unbalanced growth conditions. Optimal conditions for high PHA production were C/N ratio 50, temperature 36$\circ$C and pH 6.8. To overcome fructose inhibition on cell growth, intermittent feeding fed-batch culture technique was used. Total cell concentration was 17.4 g/l with 9.1 g/l of PHA. The purified PHA was identified PHB/HV copolymer by NMR analysis.

• KSBB Journal
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• 제22권2호
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• pp.102-108
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• 2007

제올라이트로 충진된 수직 흐름 갈대 여과상에 인공하수를 주입하면서 처리효율을 조사하였다. 여과상 표면적 $m^2$당 1일 314 L의 인공하수가 6시간마다 10분 동안 간헐적으로 주입되었다. 처리수의 pH는 원수보다 감소하였으며, 용존산소 농도는 원수보다 증가하였다. 수직 흐름 여과상에서 각 항목별 연중 평균 처리효율은 SS 89.9%, $COD_{Cr}$ 86.1%, $COD_{Mn}$ 81.2%, BOD 93.3%, T-N 34.0%, $NH_4^+$-N, 97.3%,T-P 34.6%이었다. 처리수 중 T-N의 대부분은 $NO_3^-$-N이었으며 $NO_2^-$-N의 농도는 0.10 mg/L 이하이었다. 처리효율의 계절적 차이는 없었다. 수직 흐름 칼럼실험에서 여재인 제올라이트에 흡착된 인의 형태별 함량은 모든 깊이에서 Ca-P > Fe-P > 환원가용성 Fe-P > Occluded p > Saloid P > Al-P 순이었다. 여과상 깊이별 Al-P를 제외한 모든 종류의 인화합물의 함량은 위층일수록 높았으며, 함량 비율은 깊이가 깊을수록 높아지는 경향이었다. 유기물 함량도 상층 (0$\sim$5 cm 깊이)에 가장 높았으며, 그 이하의 깊이에서는 별 변화가 없었다. 가동기간이 경과할수록 모든 깊이에서 유기물 함량이 증가하였다.

• 한국미생물·생명공학회지
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• 제29권4호
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• pp.234-239
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• 2001

천연풍미소재로 정미성 nucleotide 함량이 높은 효모추출물을 만들기 위해서 세포내 RNA 함량이 높은 효모균주 S , cerevisat MTY 62를 선별하여TRh, 당밀과 CSL배지로 발효조 회분배양과 유가배양을 수행하였다. 여러가지 단양한 간헐적 유가배양 중에서 40% 당밀과 20% CSL의농축기질액을 50ml 다섯번 공급하는 간헐적 유가배양 (IFB-IV) 에서 최대 세포농도는 33.8g-DCW/1 RNA 농도는 5221 mg/l RNA 함량은 153 mg-RNA/g DCW 값을 나타내었다. 일정속도의 유가배양에서는 기질 요액(40% molasses, 20% CSL)을 배양 9~13시간에서는 49 ml/h 13~21 이시간에서는 24 ml/h 그 이후로는 18 ml/h 로 기질공급속도를 단계적으로 감소시키는 유가배양 (CFB-III) 에서 42.7g-DCW-1 최대 세포농도를 5545 mg RNA/1의 RNA농도를 보여, 간헐적 및 일정 속도 유가배양 중에서 가장 높은 세포농도와 RNA 농도 값을 나타내었다. 그러나 RNA 함량 면에서는 가장 낮은 130 mg-RNA/g-DCW 값을 보여다. 즉 세포농도가 높을수록 RNA 함량은 감소하는 경향을 나타내었다. 이 상의 두 가지 유가배양에 대해 비증식속도 ($\mu$)에 따른 RNA 함량을 조사한 결과, $\mu$값이 증가할수록 RNA 함량도 증가하였다. 그러나 일정한 $\mu$에서는 일정속도의 기질공급 방식보다 간헐적 기질공급 방식이 더 높은 RNA 함량을 나타내었다.

• 대한유전성대사질환학회지
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• 제11권1호
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• pp.88-98
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• 2011

Lesch-Nyhan syndrome is a X-linked recessive disorder caused by a deficiency of the enzyme hypoxanthine-guanidine phosphoribosyltransferase (HPRT), enzyme to recycle purines. Case history: born induced vaginal delivery at 40 weeks complicated by premature membrane ruputure, body weight 2.820 gm. He showed failure to thrive showing severe protein aversion like milk products and pink daper. Developmental delay revealing rolling over at 10.5 month, followed by regression. Seizure at 2 months, His poor oral feeding was lifelong problem. Weak crying, spastic, choreoathetoid movement. Self mutilating behavior noted and diagnosed at age 3 years. No family history of consanguinity and neurological disorders. Method: Laboratory test, physical exam, imaging study and molecular. Clinical follow up Treat ment with allopurinol. Result: uric acid 10.5 mg/dL (N 3.5-7.9), APRT 151.1uM/ min/ml pro(25.7-101), HPRT 7.6 (N 233.5-701) and c.151C>T hemizygote (p,Arg51X). Abdominal sonogram showed staghorn calculi in both kidneys, brain MRI brain atrophy. Clinical follow up showed, seizure at 2 mo, developmental delay (head control and, rolling over at at 11mo, pointing body part at 2 yr 7 mo, eye hand coordination at 2 y 11mo,creeping at 3 y 7 mo, speaking words at 6 y 6 mo ),and developmental regression at 3 yr of age. Sleeping problem including insomnia and severe constipation. Self mutilating behavior (lip bite) started at 2.5 yr, neurologic sx including intermittent upward gaze accompanied by swallowing difficulty at 3 y 7 mo grand mal seizure at 4.5 yr and spastic extremity and trunchal hypotonia and choleoathetoid movement and ataxia at 6.5 yr. Scoliosis with severe spasticity at 9 yr 9 mo. Acute life threatening episode with irregular breathing at 9 yr and 9 mo, Emaciation and nephrolithiasis and recurrent pneumonia. Died suddenly at 10 yr 3 mo. Conclusion: life long feeding problem, chronic gut motility dysfunction, sleeping difficulty and progressing neurologic deterioration and nephrolithiasis despite normal serum uric acid maintence by allopurinol treatment.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권3호
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• pp.237-245
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• 2004

왜소악, 설하수, 구개열의 세 가지 특징적 증상과 더불어 간헐적인 청색증, 흉곽의 함몰, 기도 폐색에 의한 호흡 곤란과 수유곤란 등의 임상소견을 나타낸 3례의 Pierre Robin sequence 환아에서, 혀의 위치를 교정하기 위한 구강저골막하박리술과 혀를 전방으로 위치시켜 호흡 곤란과 기도 폐색을 예방하기 위한 설구순접합술과 동시에 시행하고 약 1년 경과 후 설구순분리술을 시행한 결과 호흡 곤란과 수유장애의 해소, 혀의 전방 재위치, 체중 증가 및 하악골의 성장 증진 등 만족할 만한 결과를 얻었다. 구강저골막하박리술과 구순접합술 동시 시술은 Pierre Robin sequence 환아에서 기도 폐색의 외과적 치료를 위한 간단하고도 신뢰할만한 방법이라고 사료된다.