• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.12
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• pp.4292-4307
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• 2021

For the research field of night infrared surveillance video, the target imaging in the video is easily affected by the light due to the characteristics of the active infrared camera and the classical ViBe algorithm has some problems for moving target extraction because of background misjudgment, noise interference, ghost shadow and so on. Therefore, an improved ViBe algorithm (I-ViBe) for moving target extraction in night infrared surveillance video is proposed in this paper. Firstly, the video frames are sampled and judged by the degree of light influence, and the video frame is divided into three situations: no light change, small light change, and severe light change. Secondly, the ViBe algorithm is extracted the moving target when there is no light change. The segmentation factor of the ViBe algorithm is adaptively changed to reduce the impact of the light on the ViBe algorithm when the light change is small. The moving target is extracted using the region growing algorithm improved by the image entropy in the differential image of the current frame and the background model when the illumination changes drastically. Based on the results of the simulation, the I-ViBe algorithm proposed has better robustness to the influence of illumination. When extracting moving targets at night the I-ViBe algorithm can make target extraction more accurate and provide more effective data for further night behavior recognition and target tracking.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.106-114
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• 2024

Fusarium head blight (FHB), predominantly caused by Fusarium graminearum and F. asiaticum, is a significant fungal disease impacting small-grain cereals. The absence of highly resistant cultivars underscores the need for vigilant FHB surveillance to mitigate its detrimental effects. In 2023, a notable FHB outbreak occurred in the southern region of Korea. We assessed FHB disease severity by quantifying infected spikelets and grains. Isolating fungal pathogens from infected samples often encounters interference from various microorganisms. We developed a cost-effective, selective medium, named BGT (Burkholderia glumae Toxoflavin) medium, utilizing B. glumae, which is primarily known for causing bacterial panicle blight in rice. This medium exhibited selective growth properties, predominantly supporting Fusarium spp., while substantially inhibiting the growth of other fungi. Using the BGT medium, we isolated F. graminearum and F. asiaticum from infected wheat and barley samples across Korea. To further streamline the process, we used a direct PCR approach to amplify the translation elongation factor 1-α (TEF-1α) region without a separate genomic DNA extraction step. Phylogenetic analysis of the TEF-1α region revealed that the majority of the isolates were identified as F. asiaticum. Our results demonstrate that BGT medium is an effective tool for FHB diagnosis and Fusarium strain isolation.

• Progress in Medical Physics
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• v.8 no.1
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• pp.53-67
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• 1997

General hospitals have being under the influence of various and specific environment of electromagnetic field. The factors are development of medical electric equipment and device with enlarged functional demands, in high power and multi-frequency. It has all of both faces, EMI(electromagnetic interference) and EMS(electromagnetic susceptibility). In additional, expansion of personal communication system(cellular phone) has many unreliable factor of using time and area, making noise of electromagnetic fields. We studied actual conditions of EMI in the medical site, where is numerous medical equipment, especially central operation room and ICU(intensive care unit), AKR(artificial kidney room : hemo-dialysis unit), etc. The influence, most of medical equipments made electromagnetic nosie has various factors in its band of frequency, harmonics and strength. In the experimental use of electro-surgical unit and cellular phone, noticeable and considerable noise of eletromagnetic fields were measured. All of that can make trouble and errors on the steadiness of bioelectrical devices. In conclusion, It is necessary to reconsiderations of reallocating EMI source vs. EMS factor, and set to definite forbiding area of using cellular phone. For maintenance of steady normal conditions, in spite of existing any other legal standards of safty level, it need considering all of alternative electromagnetic situations on a case-by-case basis.

• Journal of the Korean Geotechnical Society
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• v.29 no.8
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• pp.37-51
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• 2013

An energy pile encases heat exchange pipes to exchange thermal energy with the surrounding ground formation by circulating working fluid through the pipes. An energy pile has many advantages in terms of economic feasibility and constructability over conventional Ground Heat Exchangers (GHEXs). In this paper, a coil-type PHC energy pile was constructed in a test bed and its thermal performance was experimentally and numerically evaluated to make a preliminary design. An in-situ thermal response test (TRT) was performed on the coil-type PHC energy pile and its results were compared with the solid cylinder source model presented by Man et al. (2010). In addition, a CFD numerical analysis using FLUNET was carried out to back-analyze the thermal conductivity of the ground formation from the Ttype PHC energy RT result. To study effects of a coil pitch of the coil-type heat exchange pipe, a thermal interference between the heat exchange pipes in PHC energy piles was parametrically studied by performing the CFD numerical analysis, then the effect of the coil pitch on thermal performance and efficiency of heat exchange were evaluated. Finally, an equivalent heat exchange efficiency factor for the coil-type PHC energy pile in comparison with a common multiple U-type PHC energy pile was obtained to facilitate a preliminary design method for the coil-type PHC energy pile by adopting the PILESIM2 program.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.9
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• pp.1028-1036
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• 2008

In the OFDM system, IQ imbalance problem happens at the RF front-end of transceiver, which degrades the BER(bit error rate) performance because it affects the constellation in the received signal. Also, phase noise is generated in the local oscillator of transceivers and it destroys the orthogonality between the subcarriers. Conventional PNS algorithm is effective for phase noise suppression, but it is not useful anymore when there are jointly IQ(In-phase and Quadrature) imbalance and phase noise. Therefore, in this paper, we analyze the effect of IQ imbalance and phase noise generated in the down-conversion of the receiver. Then, we estimate and compensate the IQ imbalance and phase noise at the same time. Compared with the conventional method that IQ imbalance after IFFT is estimated and compensated in front of FFT via the feedback, this proposed method extracts and compensates effect of IQ imbalance after FFT stage. In case IQ imbalance and phase noise exist at the same time, we can decrease complexity because it is needless to use elimination of IQ imbalance in time domain and training sequences and preambles. Also, this method shows that it reduces the ICI and CPE component using adaptive forgetting factor of MMSE after FFT.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2045-2049
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• 2012

Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.685-689
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• 2013

Objective: To study the effects of down-regulation of HDAC6 expression on proliferation, cell cycling and migration of esophageal squamous cell carcinoma (ESCC) cells and related molecular mechanisms. Methods: ESCC cell line EC9706 cells were randomly divided into untreated (with no transfection), control siRNA (transfected with control siRNA) and HDAC6 siRNA (transfected with HDAC6 small interfering RNA) groups. Effects of HDAC6 siRNA interference on expression of HDAC6 mRNA and protein in EC9706 cells were investigated by semi-quantitative RT-PCR, Western blotting and immunocytochemistry methods. Effects of down-regulation of HDAC6 expression on cell proliferation, cell cycle, and cell migration were studied using a CCK-8 kit, flow cytometry and Boyden chambers, respectively. Changes of mRNA and protein expression levels of cell cycle related factor (p21) and cell migration related factor (E-cadherin) were investigated by semi-quantitative RT-PCR and Western blotting methods. Results: After transfection of HDAC6 siRNA, the expression of HDAC6 mRNA and protein in EC9706 cells was significantly downregulated. In the HDAC6 siRNA group, cell proliferation was markedly inhibited, the percentage of cells in G0/G1 phase evidently increased and the percentage of cells in S phase decreased, and the number of migrating cells significantly and obviously decreased. The mRNA and protein expression levels of p21 and E-cadherin in the HDAC6 siRNA group were significantly higher than those in the untreated group and the control siRNA group, respectively. Conclusions: HDAC6 siRNA can effectively downregulate the expression of HDAC6 mRNA and protein in EC9706 cells. Down-regulation of HDAC6 expression can obviously inhibit cell proliferation, arrest cell cycling in the G0/G1 phase and reduce cell migration. The latter two functions may be closely related with the elevation of mRNA and protein expression of p21 and E-cadherin.

• Biomolecules & Therapeutics
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• v.21 no.1
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• pp.29-34
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• 2013

The tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor family of cytokines. TRAIL selectively induces apoptotic cell death in various tumors and cancer cells, but it has little or no toxicity in normal cells. Agonism of TRAIL receptors has been considered to be a valuable cancer-therapeutic strategy. However, more than 85% of primary tumors are resistant to TRAIL, emphasizing the importance of investigating how to overcome TRAIL resistance. In this report, we have found that nemadipine-A, a cell-permeable L-type calcium channel inhibitor, sensitizes TRAIL-resistant cancer cells to this ligand. Combination treatments using TRAIL with nemadipine-A synergistically induced both the caspase cascade and apoptotic cell death, which were blocked by a pan caspase inhibitor (zVAD) but not by autophagy or a necrosis inhibitor. We further found that nemadipine-A, either alone or in combination with TRAIL, notably reduced the expression of survivin, an inhibitor of the apoptosis protein (IAP) family of proteins. Depletion of survivin by small RNA interference (siRNA) resulted in increased cell death and caspase activation by TRAIL treatment. These results suggest that nemadipine-A potentiates TRAIL-induced apoptosis by down-regulation of survivin expression in TRAIL resistant cells. Thus, combination of TRAIL with nemadipine-A may serve a new therapeutic scheme for the treatment of TRAIL resistant cancer cells, suggesting that a detailed study of this combination would be useful.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.406-414
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• 2005

The genetic instability of cancer cells may be related to inappropriately activated DNA repair pathways. In present study, the modulated expression of DNA-dependent protein kinase (DNA-PK), a major DNA repair protein, in human cancer metastatic cells was tested. The expressions of Ku70/80, regulatory subunit of DNA-PK, and the Ku DNA-binding activity in various highly metastatic cell lines were higher than those in each parental cell line. Also, the expression of DNA-PKcs, catalytic subunit of DNA-PK, and the kinase activity of the whole DNA-PK complex in highly metastatic cells were significantly increased as compared to those of parental cells, suggesting that the enhanced DNA repair capacity of metastatic cells could be associated with aberrant use of DNA repair, which may mediate tumor progression and metastatic potential. Increased EGFR (epidermal growth factor receptor) signaling has been associated with tumor invasion and metastasis, and the linkage between EGFR-mediated signaling and DNA-PK has been suggested. This study showed that PKI166, the new EGFR tyrosine kinase inhibitor, modulated the expressions of Ku70/80 and DNA-PKcs and also revealed the chemosensitization effect of PKI166 against metastatic cells may be in part due to inhibition of Ku70/80. These results suggest that interference in EGFR signaling by EGFR inhibitor resulted in the impairment of DNA repair activity, and thus DNA-PK could be possible molecular targets for therapy against metastatic cancer cells.

• Journal of Korean Society of Forest Science
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• v.89 no.2
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• pp.145-160
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• 2000

This study is focused to the national park of Korean typical Sea Hally$\check{o}$ Haesang, and its visual resources and practiced inspect course by the way of suppositions and tests, to show the visual resource management objectively, and that of qualitative basic data. Accordingly by measuring the physical amount spatial structure with the visual amount originated from the Mesh Analyzing Method and the Visual Preference from the Scenic Beauty Estimation(S.B.E.) method and analyzed the valuation of the visual resource by Iverson method. Spatial image structure measured by Semantic Differential(S.D.) Scale was shown through the factor analysis algorithm for the analyzing psychological amount and examined the flowing out of decisive factor and the objective importance related to the mutual factors by appling the measurement of the visual quality. As a national Park, the visual factors that have natural landscape harmonized with forest, sky, surface of the water, curious stones and rocks, and temples should be escalated their values affirmatively so as to be the scenery of pointed direction and enjoyable, and it is of more needed for visual resource and its' controlling technique to make artificial structures more intentional planning and systemical setting. When we are viewing the improvement for the national park along with the visual resource management, reasonable level of development is needed, because when men interference surpass plantations and leasts will be damaged and the quality of natural landscape can be lowered, so it is needed to set up a management end, tangibly or clearly; and it is permitted limit coming and going ablably by accounting the suitable number for availing. But the controling end should be set in every level, positive management, very actively within the permissive varcability. It is the main business for the national park to prevent the damage from human for their gay life or to prevent the damage of a land carpet, and to restorate for the visual resource management.