• 한국미생물·생명공학회지
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• 제29권1호
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• pp.1-11
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• 2001

Lactic acid bacteria (LAB) are widely used for various food fermentation. With the recent advances in modern biotechnology, a variety of bio-products with the high economic values have been produced using microorganisms. For molecular cloning and expression studies on the gene of interest, E. coli has been widely used mainly because vector systems are fully developed. Most plasmid vectors currently used for E, coli carry antibiotic-resistant markers. As it is generally believed that the antibiotic resistance markers are potentially transferred to other bacteria, application of the plasmid vectors carrying antibiotic resistance genes as selection markers should be avoided, especially for human consump-tion. By contrast, as LAB have some desirable traits such that the they are GRAS(generally recognized as safe), able to secrete gene products out of cell, and their low protease activities, they are regarded as an ideal organism for the genetic manipulation, including cloning and expression of homologous and heterologous genes. However, the vec-tor systems established for LAB are stil insufficient to over-produce gene products, stably, limiting the use of these organisms for industrial applications. For a past decade, the two popular plasmid vectors, pAM$\beta$1 of Streptococcus faecalis and pGK12 theB. subtilis-E. coli shuttle vector derived from pWV01 of Lactococcus lactis ssp. cremoris wg 2, were most widely used to construct efficient chimeric vectors to be stably maintained in many industrial strains of LAB. Currently, non-antibiotic markers such as nisin resistance($Nis^{r}$ ) are explored for selecting recombi-nant clone. In addition, a gene encoding S-layer protein, slp/A, on bacterial cell wall was successfully recombined with the proper LAB vectors LAB vectors for excretion of the heterologous gene product from LAB Many food-grade host vec-tor systems were successfully developed, which allowed stable integration of multiple plasmid copies in the vec-mosome of LAB. More recently, an integration vector system based on the site-specific integration apparatus of temperate lactococcal bacteriophage, containing the integrase gene(int) and phage attachment site(attP), was pub-lished. In conclusion, when various vector system, which are maintain stably and expressed strongly in LAB, are developed, lost of such food products as enzymes, pharmaceuticals, bioactive food ingredients for human consump-tion would be produced at a full scale in LAB.

• 한국식품영양학회지
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• 제30권1호
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• pp.41-50
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• 2017

This study developed a vegetable preference increase program that can be applied at the Center for Children's Foodservice Management (CCFM) for children in child-care facilities and kindergarten and the training performance was evaluated by 192 5-year old children. Teachers considered 'disliked food' ($3.23{\pm}0.85/out$ of 5) as the most serious nutritional problem and typical disliked food was 'vegetables' (54.2%). Based on this finding, to increase the preference for vegetables, a five-phase education program was developed. In this program, each phase was composed of activities to increase children's interest in vegetables by using each sense out of the five senses. Center dieticians had visited and conducted the program in 12 facilities. By using an illustration assessment tool, the training performance was evaluated by comparing the children's knowledge level and their preference changes in vegetables. Consequently, as examined by 10 questions on the knowledge of vegetables, trained children's (in total 192) knowledge of vegetables had considerably increased (p<0.01, p<0.001), and their preference for 15 out of the 16 vegetables, except for carrots, also showed a considerable preference increase after the training (p<0.01, p<0.001). Therefore, this program is considered to have contributed to reducing disliked vegetable eating behaviors.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.93-93
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• 2010

Semiconductor nanocrystal quantum dots (NQDs) have recently attracted considerable interest for use in photovoltaics. Band gaps of NQDs can be tuned over a considerable range by varying the particle size thereby allowing enhance absorption of solar spectrum. NQDs, synthesized using colloidal routes, are solution processable and promise for a large-area fabrication. Recent advancements in multiple-exciton generation in NQD solutions have afforded possible efficiency improvements. Various architectures have attempted to utilize the NQDs in photovoltaics, such as NQD-sensitized solar cell, NQD-bulk-heterojuction solar cell and etc. Here we have fabricated CdSe NQDs with the band gap of 1.8 eV to 2.1 eV on thin-layers of p-type organic crystallites (1.61 eV) to realize a donor-acceptor type heterojuction solar cell. Simple structure as it was, we could control the interface of electrode-p-layer, and n-p-layer and monitor the following efficiency changes. Specifically, surface molecules adsorbed on the NQDs were critical to enhance the carrier transfer among the n-layer where we could verify by measuring the photo-response from the NQD layers only. Further modifying the annealing temperature after the deposition of NQDs on p-layers allowed higher conversion efficiencies in the device.

• 한국결정성장학회지
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• 제19권3호
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• pp.107-115
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• 2009

Particular interest in the role of convection in vapor crystal growth has arisen since some single crystals under high gravity acceleration of $10g_0$ appear considerably larger than those under normal gravity acceleration ($1g_0$). For both ${\Delta}T=60\;K$ and 90 K, the mass flux increases by a factor of 3 with increasing the gravity acceleration from $1g_0$ up to $10g_0$. On the other hand, for ${\Delta}T=30\;K$, the flux is increased by a factor of 1.36 for the range of $1g_0{\leq}g{\leq}10g_0$. The maximum growth rates for $1g_0$, $4g_0$, $10g_0$ appear approximately in the neighborhood of y = 0.5, and the growth rates shows asymmetrical patterns, which indicate the occurrence of either one single or more than one convective cell. The maximum growth rate for $10g_0$ is nearly greater than that for $1g_0$ by a factor of 2.0 at $P_B=20\;Torr$. For three different gravity levels of $1g_0$, $4g_0$ and $10g_0$, the maximum growth rates are greater than the minimum rates by a factor of nearly 3.0, based on $P_B=20\;Torr$. The mass flux increases with increasing the gravity acceleration, for $1g_0{\leq}g_y{\leq}10g_0$, and decreases with increasing the partial pressure of component B, xenon (Xe), $P_B$. The $|U|_{max}$ is directly proportional to the gravity acceleration for $20\;Torr{\leq}P_B{\leq}300\;Torr$. As the partial pressure of $P_B$ (Torr) decreases from 300 Torr to 20 Torr, the slopes of the $|U|_{max}s$ versus the gravity accelerations increase from 0.1 sec to 0.17 sec. The mass flux of $Hg_2Cl_2$ is exponentially decayed with increasing the partial pressure of component B, $P_B$ (Torr) from 20 Torr up to 300 Torr.

• Annals of Clinical Neurophysiology
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• 제1권2호
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• pp.160-167
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• 1999

Although somatosensory evoked potentials(SSEPs) have been utilized as the useful diagnostic tools in evaluating the wide variety of pathological conditions, such as focal lesions affecting the somatosensory pathways, demyelinating diseases, and detecting the clinically occult abnormality, their neural generators is still considerably uncertain. To appreciate the basis for uncertainties about the origins of SSEPs, consider criteria that must be met to establish a causal relationship between activity in a neural structure and a spine/ scalp-recorded potential. Electrode locations and channel derivations for SSEPs recordings are based on two principles:(1) the waveforms are best recorded from electrode sites on the body surface closest to the presumed generator sources along the somatosensory pathways, and(2) studies of the potential-field distribution of each waveform of interest dictate the best techniques to be used. In this article, authors will describe followings focused on ;(1) the concepts of near field potentials(NFPs) and far field potentials(FFPs) - the voltage of NFPs is highly dependent upon recording electrode position, FFPs are unlike NFPs in that they are widely distributed, their latencies and amplitudes are independent of recording electrode.(2) appropriate montage settings to detect the significant potentials in the median nerve and posterior tibial nerve SSEPs(3) neural generators of various potentials(P9, N13, P14, N18, N20, P37) and their clinical significance in interpretating the results of SSEPs. Especially, Characteristics of N18(longduration, small superimposed inflection) suggested that N18 is a complex wave with multiple generators including brainstem structures and thalamic nuclei. And N18 might be used as the parameter of braindeath. Precise understanding on these facts provide an adequate basis utilizing SSEPs for numerous clinical purposes.

• 대한지역사회작업치료학회지
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• 제4권2호
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• pp.23-30
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• 2014

목적 : 본 연구는 국내 작업치료 인지도 및 관심도에 대해 알아보고, 그에 따른 결과를 통해 작업치료 활성화를 위한 기초 자료를 구축하고자 한다. 연구방법 : 2013년 10월부터 2013년 12월까지 대학생과 일반인 총 308명을 대상으로 직접 만나 설문조사 하였다. 분석방법은 SPSS 18.0을 이용하여 t검정, 분산분석, 사후검정, 빈도분석, 기술통계를 사용하였다. 결과 : 작업치료의 인식도에 관하여 연령과 직업에 따라 유의한 차이가 나타났고(p<.01), 작업치료에 관하여 알고 있는 사람들은 작업치료와 관련된 사람이 있거나(33.5%), 언론매체를 통하여 알게 되는 것으로 나타났다(37.1%). 작업치료에 관하여 알아보고 싶다는 긍정적 응답이 높게 나타났으며(53%), 활성화를 위한 방안으로는 홍보(63.5%)가 가장 효과적인 것으로 나타났다. 결론 : 작업치료에 관한 인지도는 부족하였으나 추후 본인이 장애를 입게 된다면 작업치료를 받고 싶다는 응답이 높고 작업치료를 알고 싶다는 대답이 높게 나타나는 것으로 미루어 보아 높은 관심도를 갖고 있는 것으로 조사되었다. 작업치료를 알리기 위해서 적극적인 홍보를 인터넷 등의 언론매체나 지인을 통한 홍보가 효과적일 것으로 사료된다.

• 한국방사선학회논문지
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• 제12권2호
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• pp.193-199
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• 2018

본 연구는 총 15명의 정상인 지원자를 대상으로 1.5T와 3.0T의 자기공명영상기기(Philips, Medical System, Achieva)를 이용하여 강자성 인공물을 최소화하기 위한 최적의 Tesla를 알아보고자 하였다. 평가는 신호대 잡음비 평가, 강자성 인공물이 형성된 부위의 길이와 Histogram을 평가하였다. 분석방법으로는 T1, T2 sagittal 영상의 Background의 4 부위에 관심영역을 설정하고 L3, L4, L5의 각 추체부에 관심영역을 설정한 후 신호대잡음비 값을 측정하였고, 인공물이 형성된 3 부위에 관심영역을 설정하고 길이 값을 측정하였고, Image J 프로그램을 이용해 인공물 영역의 히스토그램 분포 값을 측정하였다. 본 실험에 대한 결과로 신호대 잡음비 평가에서 L3에서는 1.5T와 3.0T 사이에 별 차이가 없는 것으로 나타났고, L4, L5에서는 큰 차이가 있는 것으로 나타났다(p<0.05). 강자성 인공물이 형성된 3 부위의 길이는 3.0T에 비해 1.5T가 더 짧아 주변조직에 대한 진단적 정보를 더 얻을 수 있는 것으로 나타났다(p<0.05). Histogram평가에서는 3.0T보다 1.5T가 Count값이 높게 나타났다(p<0.05). 결론적으로 1.5T와 3.0T의 신호대 잡음비, 강자성 인공물의 길이, Histogram을 비교 평가해 봤을 때, Spine MRI 검사 시에 PLIF 등 디스크 수술을 받은 환자에게는 Low Tesla로 검사가 최적의 영상정보를 얻을 수 있었다.

• 보건교육건강증진학회지
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• 제27권1호
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• pp.49-60
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• 2010

Objectives: The purpose was to evaluate the effectiveness of participatory road safety education program for the lower grade in elementary schools. This program was developed based on the Activated Health Education model. Methods: Study design was compromise experimental group pre-post design. 456 students in 8 schools were nonrandomly assigned to study group(n=224) or control(n=232). The students finished self-administered questionnaire before and after education. Collected data was analyzed by using the SPSS. Results: 1. The common relevant factors in road safety knowledge and attitude were 'residential state' and 'experience of traffic accident'. 2. The knowledge showed that the case and control scored at 6.48 and 6.41 points before. After this intervention, the case and control scored at 8.38 and 6.51. The difference of the case was significant(p<0.001). 3. The attitude showed that the case and control scored at 19.67 and 19.63 before. After this, the case and control scored at 19.86 and 19.63. The difference of the case was significant(p<0.05). Conclusion: In order to implement the road safety education, children's socio-demographic characteristics were considered. Because this education was effective in both improving knowledge and attitude and bringing interest, various participatory program will be applied in road safety education for children.

• 반도체디스플레이기술학회지
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• 제22권3호
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• pp.101-105
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• 2023

Organic photo Diodes (OPDi) give multiple advantages in the growing interest of the flexible optoelectronic devices. Organic semiconductors are freeform as they can deposit on any substrate, so it could be flexible. But the inorganic material photodiodes (PDs) are usually fabricated on silicon wafers which are solid. So, normally PDs are inflexible. By those reasons, we decided to make the vacuum deposited small molecule OPDi. We have investigated the OPDi's J-V characteristic by changing the thickness of p-type layer of OPDi. This device consists of indium-tin-oxide (ITO) / 2,3:6,7-dibenzanthracene (pentacene) / buckminsterfullerene (C60) / aluminum (Al). Its J-V characteristics were measured in the probe station(4156C) that can give dark condition while measuring. And for the luminance characteristics, the photocurrent was measured with the bright halogen lamp and a probe station.

• 원예과학기술지
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• 제30권5호
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• pp.586-595
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• 2012

본 연구는 실제 재배농가에서 실시되는 엽채류 수경재배를 이용한 원예치료 프로그램을 통해 지적 장애학생의 직업적응력을 향상시키고자 실시하였다. 직업적응훈련모델과 제 7차 특수학교 직업교과과정 농업교과의 경제작물영역에서 수경재배의 특성과 기초적인 이론을 바탕으로 엽채류(상추, Lettuce (Lactuca sativa L. 'Asia Heuk Romaine')) 수경재배를 이용한 원예치료 프로그램(총 22회기)을 개발하였다. 인천광역시 K구에 위치한 특수학교 전공과에 재학중인 1-2학년 지적 장애학생 14명(남 10명, 여 4명)을 대상으로, 학교와 가까이 위치하고 있는 인천시 K구에 위치한 채소류 전문생산농가에서 실시되었다. 본 프로그램은 2011년 9월부터 12월까지 주 2회(회기 당 평균 60분) 전공과 교육과정 내 실습교육의 일부로 진행되었다. 수경재배를 이용한 원예치료 프로그램의 효과를 검증하기 위해서 담임교사 또는 원예치료사가 원예치료 프로그램 실시 전과 후에 실험에 참여한 장애학생들을 대상으로 신경근육발달능력(McCarron assessment neuromuscular development), 정서적 행동능력(emotional behavioral checklist), 대인관계전략(interpersonal negotiation strategies), 직업 흥미도 검사(KEPAD pictoral vocational interest test)를 실시하였다. 그 결과, 수경재배를 이용한 원예치료 프로그램 실시 후에 지적 장애학생들의 운동능력(P= 0.002), 정서적 행동능력(P= 0.00), 대인관계전략(P = 0.05)이 유의하게 향상된 것으로 나타났다. 그러나 직업 흥미도에 대한 통계적 유의성은 나타나지 않았다. 결론적으로, 본 수경재배를 이용한 원예치료 프로그램은 지적 장애학생들의 직업적응력 증진에 영향을 미치는 운동능력과 정서적 행동능력 및 대인관계전략에 긍정적인 효과가 있는 것으로 조사되었으며, 수경재배는 대체로 단순하고 쉬운 작업과정으로, 지적 장애인의 직업영역으로써 적합하며, 직무수행에 있어서도 비교적 어려움이 적은 직종이라 판단된다. 추가적으로 직업적응력 증진을 위해 실제 현장에서 다양한 종류의 엽채류를 재배하는 수경재배 프로그램의 개발 및 적용이 필요할 것이다.