• Biomolecules & Therapeutics
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• v.26 no.2
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• pp.201-209
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• 2018

G protein-coupled receptor 119 (GPR119) is expressed in the pancreas and gastrointestinal tract, and its activation promotes insulin secretion in the beta cells of the pancreatic islets as well as the secretion of glucagon-like peptide-1 (GLP-1) in intestinal L cells, consequently improving glucose-stimulated insulin secretion. Due to this dual mechanism of action, the development of small-molecule GPR119 agonists has received significant interest for the treatment of type 2 diabetes. We newly synthesized 1,2,4-triazolone derivatives of GPR119 agonists, which demonstrated excellent outcomes in a cyclic adenosine monophosphate (cAMP) assay. Among the synthesized derivatives, YH18968 showed cAMP=2.8 nM; in GLUTag cell, GLP-1secretion=2.3 fold; in the HIT-T15 cell, and insulin secretion=1.9 fold. Single oral administration of YH18968 improved glucose tolerance and combined treatment with a dipeptidyl peptidase 4 (DPP-4) inhibitor augmented the glucose lowering effect as well as the plasma level of active GLP-1 in normal mice. Single oral administration of YH18968 improved glucose tolerance in a diet induced obese mice model. This effect was maintained after repeated dosing for 4 weeks. The results indicate that YH18968 combined with a DPP-4 inhibitor may be an effective therapeutic candidate for the treatment of type 2 diabetes.

• Biomolecules & Therapeutics
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• v.26 no.5
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• pp.481-486
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• 2018

Cosmetics are primarily applied to the skin; therefore, the association of cosmetic dyes with skin diseases or inflammation is a topic of great interest. Thymic stromal lymphopoietin (TSLP) is an interleukin 7-like cytokine that activates dendritic cells to promote Th2 inflammatory immune responses. TSLP is highly expressed in keratinocytes under inflammatory conditions, which suggests that it may play a critical role in the development of skin diseases, such as atopic dermatitis. Therefore, we investigated whether cosmetic dyes influenced the production of TSLP by keratinocytes. Phloxine O, also known as D&C Red No.27, is one of the most common red synthetic pigments and is widely used in colored cosmetics. Our results showed that Phloxine O downregulated phorbol 12-myristate 13-acetate-induced production of TSLP in a murine keratinocyte cell line (PAM212). Phloxine O also suppressed TSLP expression in KCMH-1 cells, which are mouse keratinocytes that constitutively produce high levels of TSLP. To investigate the in vivo effects of Phloxine O, we induced TSLP expression in mouse ear skin by topically applying MC903, a vitamin D3 analogue that is a well-known inducer of atopic dermatitis-like symptoms. Topical application of Phloxine O prevented MC903-induced TSLP production in mouse ear skin, attenuated the acute dermatitis-like symptoms and decreased serum IgE and histamine levels in mice. Suppression of TSLP expression by Phloxine O correlated with reduced expression of OX40 ligand and Th2 cytokines in mouse ear skin. Our results showed that Phloxine O may be beneficial to prevent dermatitis by suppressing the expression of TSLP and Th2 cytokines in skin.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.307-313
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• 2003

Fluorescent differential display (FDD) is a method for identifying differentially expressed genes in eukaryotic cells. The mRNA FDD technology works by systematic amplification of the 3' terminal regions of mRNAs. This method involve the reverse transcription using anchored primers designed to bind 5'boundary of the poly A tails, followed by polymerase chain reaction (PCR) amplification with additional upstream primers of arbitrary sequences. The amplified cDNA subpopulations are separated by denaturing polyacrylamide electrophoresis. To identify the genes involved in the development of first trap leaf, we applied a FDD method using mRNAs from leaf base, first trap leaf and flower tissue, respectively. We screened several genes that expressed specifically in first trap leaf. Nucleotide sequence analysis of these genes revealed that these were protease inhibitor (PI), myo-inositol-1-phosphate synthase and lipocalin-type prostaglandin D synthase. Northern blot analysis showed that these genes were expressed specifically in first trap leaf (in vivo and in vitro). FDD could prove to be useful for simultaneous scanning of transcripts from multiple cDNA samples and faster selection of differentially expressed transcripts of interest.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.6
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• pp.543-551
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• 2017

A campus guide robot was developed that can autonomously guide people through a university campus. The robot is able to evaluate its location using Differential Global Positioning System (DGPS) and Dead-Reckoning using the encoders mounted on its wheels. The robot can navigate autonomously along a guide route that is set in advance. A new position-based guidance approach was suggested. Unlike the conventional method of setting the guide sequence in advance, the robot acquires guidance by judging whether there is guide information corresponding to its current position. The robot searches guide information from the guide database while it moves along the guide path autonomously. If there is any guide information available around the location of the robot, then it performs guide functions. We also suggested an effective guide scenario that can maximize the interest of people. The performance of the robot was tested through sets of experiments in a true campus environment.

• Korean Journal of Organic Agriculture
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• v.20 no.3
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• pp.267-282
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• 2012

In this study, RPS system, one of the renewable energy support systems, is utilized for economic analysis of solar generation equipment and the fuel cost savings plan for controlled horticulture farms with high fuel-cost dependency and facility applicability were evaluated. On the exterior of the upper layer of glass greenhouse (9917$m^2$) of controlled horticulture farms using bunker C oil, half of the area (4958$m^2$) was utilized for theoretical installation and operation of 450kW-level solar power generator, and as the result, first, the effect of investment cost only of solar generation system was found to be quite excellent, but it was analyzed that there were limits to saving the fuel costs of the controlled horticulture farms. Second, when geothermal system was first introduced in the farm and solar system was additionally introduced, it was analyzed that the effect of introducing solar system was excellent. In order to apply such effects to the sites of farming, partial supplementation of RPS system which is being uniformly applied regardless of the purpose of renewable energy is necessary. When the subject of use directly install facilities where it is directly connected to national added-value such as food security created by the farming industry, it is necessary to introduce appropriate system that corresponds to such. Moreover, it was studied that the quick development of demonstrative complex that can practically evaluate the applicability of renewable energy in farming industry and interest and preparation of related institutions in financial support structure for its site application would lead to success.

• Journal of Cadastre & Land InformatiX
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• v.49 no.2
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• pp.169-185
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• 2019

While images and aerial photographs using conventional satellites have the advantage of providing data in a vast area, there is a difficult aspect: the limitations of filming and processing data in a particular region at a desired time and the repetitive filming of a short cycle. With the development of many new technologies to overcome these shortcomings, methods of building cadastral information are changing rapidly. In particular, unmanned aerial vehicles that deploy cadastral information quickly and accurately using UAV have increased interest in technology that obtains cadastral information. Therefore, the purpose of this study was to suggest the application of cadastral measurement tasks in areas subject to cadastral measurement using UAV. To this end, the Commission decided to compare and analyze the accuracy of high-resolution images produced by observation area and apply them to existing cadastral work using verified images and cadastral data. In this study, we will analyze the applicability of UAVs to their cadastral survey by analyzing the current status of legislation related to cadastral survey and the technical characteristics of UAVs and propose technological, legal and institutional improvement measures for introduction based on them.

• Korean Journal of Animal Reproduction
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• v.21 no.1
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• pp.1-7
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• 1997

Recently the production of transgenic animals to express foreign proteins in mammary glands has been a routine procedure. However, it still takes a considerable time and effort, and is faced with various technical challenges until the protein of interest is successfully made. Thus, a development of an a vitro model for mamm a ary-specific gene expression for recombinant genes was carried out in this study. To this end, bovine $\alpha$$_S1$ casein cDNA was inserted at the multiple cloning site of pMSG vector under the control of MMTV promoter. MCF$_7$ cells were tran sfected with pMSG $\alpha$$_S1$ CN by CaP0$_4$ precipitation. Transfectants were selected in HAT medium and induced with dexamethasone. The cells were analyzed with chicken anti-casein and FITC-labeled rabbit anti-chicken antibodies. The results showed that dexamethasone induced 30-40 fold increase in the MMTV- $\alpha$$_S1$ casein e expression. Therefore MCF$_7$ cells, which have multiple steroid receptors, along with pMSG vector can be used as an in vitro model for the study of mammary-specific gene expression.

• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.263-271
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• 2009

Cardiovascular diseases (CVDs) are one of the most cause of death around the world and fields of interest for cardiac stem cells. Also, current use of terminally differentiated adult cardiomyocytes for CVDs has limited regenerative capacity therefore any significant cell loss may result in the development of progressive heart failure. Human embryonic stem cells (hESCs) derived from blastocyst-stage embryos spontaneously have ability to differentiate via embryo-like aggregates (endoderm, ectoderm and mesoderm) in vitro into various cell types including cardiomyocyte. However, most effective molecule or optimized condition which can induce cardiac differentiation of hESCs is rarely studied. In this study, we developed both spontaneous and inductive cardiomyocyte-like cells differentiation from hESCs by treatment of induced-factors, 5-azacytidine, BMP-4 and cardiogenol C. On the one hand, spontaneous and inductive cardiomyocyte-like cells showed that cardiac markers are expressed for further analysis by RT-PCR and immunocytochemistry. Interestingly, BMP-4 greatly improved homogeneous population of the cardiomyocyte-like cells from hESCs CHA15 and H09. In conclusion, we verified that spontaneously differentiated cells showed cardiac specific markers which characterize cardiac cells, treated extrinsic factors can manage cellular signals and found that hESCs can undergo differentiation into cardiomyocytes better than spontaneous group. This finding offers an insight into the inductive factor of differentiated cardiomyocytes and provides some helpful information that may offer the potential of cardiomyocytes derived from hESCs using extrinsic factors.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.7 no.4
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• pp.12-18
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• 2011

The renewed global interest in nuclear power has arisen from the need to reduce greenhouse gas emissions and to provide sufficient electricity for a growing global population. Many countries with nuclear power plants (NPPs) are still implementing license extensions of 10~20 years, and even consideration is being given to the concept of life-beyond-60, a further period of license extension from 60 to 80 years. To solve the materials aging problem is integral to its success. A foundation for effective aging management of nuclear power plants is that aging is properly taken into account at each stage of a plant's lifetime, i.e. in design, manufacture, construction and operation including long term operation and decommissioning. To evaluate the plant aging phenomena, a lot of background information such as materials and environment of the parts of the reactor and plant systems is needed by the experts. Information on degradation mechanisms is also used. In this paper, a regulation on the integrated materials aging management for nuclear facilities is proposed. The proposed regulation identifies key elements of effective aging management for nuclear power plants and provides the requirements on aging management for nuclear facilities throughout all stages of the lifetime of the plant.

• Korean Journal of Community Nutrition
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• v.22 no.2
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• pp.115-126
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• 2017

Objectives: The purpose of the study was to develop communication strategies for effective nutrition education targeting pregnant women and to create nutrition education contents. Methods: The format and the contents of online resources on nutrition information for pregnant women provided by reliable institutions were analyzed. Possible solutions to overcome barriers of nutrition education as well as communication strategies for effective nutrition education were identified by a brainstorming process. Based on the communication strategies, contents for nutrition education were created. Understandability, level of interest, applicability to daily life, harmony of text and illustration, and overall satisfaction of the contents were evaluated by dietitians and pregnant women. Results: The four communication strategies were developed; (1) to focus on a few important messages, (2) to provide evidence-based information, (3) to create illustrations or infographics with a minimum amount of text, and (4) to provide tips on how to improve the current diet options. Based on these strategies, the contents were focused on three important nutrients for pregnant women, folate, iron, and calcium. The percentages of the recommended nutrient intakes of the three nutrients on selected menu and its improved version by adding a dish or changing a dish into another dish were calculated and provided. Finally, the contents were delivered as illustrations with a minimum amount of text. Overall, dietitians and pregnant women were satisfied with the contents. Conclusions: The contents developed in this study can be used in a pamphlet or a pregnancy diary, or can be shared in social networking services. Further contents on other nutrients and various menu are expected to be developed using these communication strategies.