• Archives of Pharmacal Research
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• 제14권3호
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• pp.271-278
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• 1991

To investigate further whether the effects of the dihydropyridine (DHP) drugs on calcium channels are related to those of these drugs on muscarinic receptors, the binding characteristics of the DHP calcium channel agonist, Bay K 8644, on muscarinic receptors and calcium channels were compared to those of the DHP calcium channel antagonists, nicardipine and nimodipine in the dog cardiac sarcolemma. Bay K 8644, nicardipine and nimodipine inhibited the specific $[^3H]$QNB binding with $K_i$ values of 16.7\mu{M}$, 3.5\mu{M}$ and 15.5\mu{M}$ respectively. Saturation data of $[^3H]$QNB binding with $K_i$ VALUES OF 16.7\mu{M}$ 3.5\mu{M}$ and 15.5\mu{M}$ respectively. Saturation data of $[^3H]$QNB binding in the presence of these DHP drugs showed this inhibition to be competitive. Bay K 8644, like nicardipine and nimodipine, blocked the binding of $[^3H]$nitrendipine to the high affinity DHP binding sites, but atropine did not, indicating that the muscarinic receptors and the DHP binding sites m but atropine did not, indicating that the muscarinic receptors and the DHP bindings sites on calcium channels are distinct. The $K_i$ value of Bay K 8644 for the DHP binding sites was 4nM. Nicardipine and nimodipine $(K_i:0.1-0.2\;nM)$ were at least 20 times more potent than Bay K 8644 in inhibiting $[^3H]$ nitrendipine binding. Thus, the muscarinic receptors were about 4000 times less sensitive than thes high afinity DHP binding sites to Bay K 8644. These results suggest that the DHP calcium agonist Bay K 8644 binds directly to the muscarinic receptors but its interaction with the muscarinic receptors is not related to its binding to the DHP binding sites on calcium channels.

• 한국콘텐츠학회논문지
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• 제9권5호
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• pp.332-336
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• 2009

유전체 분석에서 중요한 부분 중 하나는 기능이 알려지지 않은 미지 단백질에 대한 기능 예측이다. 단백질-단백질 상호작용 네트워크를 분석하는 것은 미지 단백질에 대한 기능을 보다 쉽게 예측할 수 있게 한다. 단백질-단백질 상호작용 네트워크로부터 미지 단백질의 기능을 예측하기 위한 다양한 연구들이 시도되어 왔다. 카이-제곱(Chi-square) 방식은 단백질-단백질 상호작용 네트워크를 통해 기능을 예측하고자 하는 연구 중 대표적인 방식이다. 하지만 카이-제곱 방식은 네트워크의 토폴로지를 반영하지 않아 네트워크 크기에 따라 예측의 정확성이 떨어지는 문제점이 있다. 따라서 본 논문에서는 카이-제곱 방식을 보완하여 정확성을 높인 새로운 기능 예측 방법을 제안한다 이를 위해 MIPS, DIP 그리고 SGD와 같은 공개된 단백질 상호작용 데이터베이스들로부터 데이터를 수집하여 분석하였다. 그리고 제안된 방식의 우수성을 입증하기 위해 각 데이터베이스들에 대해 카이-제곱방식과 제안하는 보완된 카이-제곱(Modified Chi-square)방식으로 예측해보고 이들의 정확성을 평가하였다.

• 한국게임학회 논문지
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• 제11권4호
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• pp.37-48
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• 2011

최근 많은 사람들이 온라인 게임을 즐김에 따라 플레이어의 온라인 게임 이용 시간을 늘리기 위해 새로운 형태의 다양한 게임을 시장에 출시하고 있다. 하지만 왜 플레이어가 온라인 게임을 지속적으로 플레이하는지에 대한 연구는 그렇게 많지 않다. 본 연구는 플레이어의 온라인 게임 이용 시간의 주요한 요인이 되는 즐거운 경험의 이유를 분석하고자 한다. 이를 위해 본 연구에서는 몰입의 경험과 고객충성도간의 관계를 기초로 몰입을 증가시키기 위한 내적, 외적 보상의 종류에 대해 분석하였다. 먼저 Personal Interaction을 통해 플레이어가 얻고자 하는 외적 보상은 문제해결에 따른 보상의 형평성이며, 이를 통해 플레이어는 현재 자신이 목표성취를 잘 하고 있다고 느끼는 인지적 성과를 높게 경험하는 것으로 분석되었다. 두 번째, Social Interaction을 통해 플레이어는 타 플레이어로부터 긍정적인 평가를 받기를 원하며, 이를 통해 자아존중감이 높아지는 것으로 분석되었다. 결국 인지적 성과와 자아존중감은 온라인 게임을 통해 플레이어에게 몰입을 경험하게 만드는 주요한 요인이며, 이를 통해 게임을 계속 이용하겠다는 고객 충성도가 높아진다는 것을 알 수 있었다.

• 복식문화연구
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• 제25권1호
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• pp.88-103
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• 2017

The rapid growth in the popularity of social media sites has meant that social media has become an important communication channel for brands. Brands create brand pages on social media to cultivate positive and strong relationships with consumers. This study seeks to enhance our understanding of how fashion brand pages in social media foster consumer-brand relationships by exploring the factors that affect the development of consumer-brand relationships. Drawing on the parasocial interaction theory, this study proposes parasocial interaction as a key factor of this process. Specifically, this study proposes four antecedents (vividness, interactivity, social presence, and focused attention) of consumers' parasocial interaction with brand pages, which further affects consumer responses in terms of affective engagement and brand loyalty. An online survey was administered with consumers who have followed and visited at least one fashion brand page via a social networking site (SNS). Structural equation modeling revealed that consumers' perceptions of vividness, interactivity, social presence, and focused attention on a fashion brand page positively affected their feelings towards parasocial interaction with the brand page, which in turn led to their affective engagement with the brand page and consequent brand loyalty. These findings suggest that consumers build relationships of varying degrees with brand pages in a similar manner to that with people, which leads to their development of a positive relationship with the brand. This study concludes with discussions and practical implications.

• 인터넷정보학회논문지
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• 제16권4호
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• pp.101-110
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• 2015

$21^{st}$ century has seen a tremendous advancement in the field of Information and Communication Technology. The commercial utilization of Internet tools and technologies has brought change in the traditional working environment. The functioning in an electronic environment is far more complicated than working in traditional environment and this brings many challenges, threats and potential problems. In this paper we analyze the challenges, threats and potential problems in the working environment of Pokhara University due to application of Internet tools and technology. We have chosen four basic areas on the basis of use that include web resources, electronic mails, social sites and P2P technologies. The main focus is to analyze the impact on work place productivity, social interaction, use and abuse of technology and legal issues associated with them. We, finally summarize the findings and offer solutions and recommendations from the observed data based on direct survey conducted in Pokhara University, Nepal.

• Mass Spectrometry Letters
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• 제9권3호
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• pp.67-72
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• 2018

Alkaline phosphatase (AP) is a membrane-bound glycoprotein that is widely distributed in the plasma membrane of cells of various organs and also found in many organisms from bacteria to humans. The complete amino acid sequence and three-dimensional structure of human placental alkaline phosphatase have been reported. Based on the literature data, AP consists of two presumptive glycosylation sites, at Asn-144 and Asn-271. However, it only contains a single occupied N-linked glycosylation site and no occupied O-linked glycosylation sites. Hydrophilic interaction chromatography (HILIC) has been primarily employed for the characterization of the glycan structures derived from glycoproteins. N-glycan structures from human placental alkaline phosphatase (PLAP) were investigated using HILIC-Orbitrap MS, and subsequent data processing and glycan assignment software. 16 structures including 10 sialylated N-glycans were identified from PLAP.

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2409-2413
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• 2007

Time resolved phosphorescence of Dibromobenzophenone (DBBP) choleic acid crystal was observed at 4.2 K as functions of excitation energy and delay time. The experimental results reveal that the energy transfer efficiency is dependent on the excitation energy, i.e. the density of acceptors sites. As the excitation energy or delay time increases, the resonance phosphorescence does not broaden and shift gradually, rather a broad luminescence band develops about 290 cm?1 to lower energy of the resonance phosphorescence. The observation implies that energy transfer from high to low energy sites in this system is controlled by emission of phonons or vibrons. The data of time resolved experiments were analyzed in terms of a mechanism involving direct donor-acceptor excitation transport by exchange coupling. It was concluded that an isotropic twodimensional exchange interaction topology is consistent with energy transfer in this system.

• Journal of Information Science Theory and Practice
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• 제3권4호
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• pp.19-34
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• 2015

Evaluation of retrieval systems is mostly limited to laboratory settings and rarely considers changes of performance over time. This article presents an evaluation of retrieval systems for internal Web site search systems between the years 2006 and 2011. A holistic evaluation methodology for real Web sites was developed which includes tests for functionality, search quality, and user interaction. Among other sites, one set of 20 Web site search systems was evaluated three times in different years and no substantial improvement could be shown. It is surprising that the communication between site and user still leads to very poor results in many cases. Overall, the quality of these search systems could be improved, and several areas for improvement are apparent from our evaluation. For a comparison, Google’s site search function was also tested with the same tasks.

• The Korean Journal of Ecology
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• 제23권4호
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• pp.309-313
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• 2000

Since specific types and physical characteristics of anuran calls can represent reproductive qualities of a calling male, anuran calls have been studied in context of mate choice. We in here report call types of Rana dybowskii and their variations from two different recording sites. Calls of R. dybowskii are classified to three types based on their physical characteristics and behavioral observations; 'A-call'functions to advertise locations of a calling male and to attract potential mates. A territorial male emits 'B-call'only during male-male interaction. 'C -call'is released when a male frog was clasped by another male. The A-call from two recording sites, Miwon and Wonju, shows high variations in their call characteristics.

• Plant Resources
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• 제8권3호
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• pp.175-180
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• 2005

Thin sections of cellulose fibers were incubated with an endo- and an exoglucanase labeled with gold particles of differing sizes. The hydrolytic sites were then visualized under transmission electron microscopy (TEM). The potential interaction between the ${\beta}$-1, 4-glucan substrates and the endo- and the exoglucanases was investigated using cellulosic and lignocellulosic substrates. The simultaneous visualization was very successful in distinguishing preferred substrates for each cellulase in lignocellulosic substrates. When plant lignocellulose was preincubated with endocellulase, density of the gold labeling greatly increased suggesting that preliminary exposure of lignocellulosic material to endocellulase may have enhanced the accessibility of the substrate to endocellulase and exocellulase. This result provided a plausible explanation for the observed endo/exo cellulase co-hydrolysis.